One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence
Identifieur interne : 000F80 ( Istex/Corpus ); précédent : 000F79; suivant : 000F81One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence
Auteurs : Jean C. Bés ; Jean Tkaczuk ; Kimberly A. Czech ; Mathieu Tafani ; Raymond Bastide ; Claude Caratero ; George D. Pappas ; Yves LazorthesSource :
- Cell Transplantation [ 0963-6897 ] ; 1998.
English descriptors
Abstract
The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord [14, 19, 36]. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.
Url:
DOI: 10.1016/S0963-6897(97)00161-9
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Bés</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Tkaczuk, Jean" sort="Tkaczuk, Jean" uniqKey="Tkaczuk J" first="Jean" last="Tkaczuk">Jean Tkaczuk</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Czech, Kimberly A" sort="Czech, Kimberly A" uniqKey="Czech K" first="Kimberly A." last="Czech">Kimberly A. Czech</name><affiliation><mods:affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</mods:affiliation></affiliation></author><author><name sortKey="Tafani, Mathieu" sort="Tafani, Mathieu" uniqKey="Tafani M" first="Mathieu" last="Tafani">Mathieu Tafani</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Bastide, Raymond" sort="Bastide, Raymond" uniqKey="Bastide R" first="Raymond" last="Bastide">Raymond Bastide</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Caratero, Claude" sort="Caratero, Claude" uniqKey="Caratero C" first="Claude" last="Caratero">Claude Caratero</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Pappas, George D" sort="Pappas, George D" uniqKey="Pappas G" first="George D." last="Pappas">George D. 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Bés</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Tkaczuk, Jean" sort="Tkaczuk, Jean" uniqKey="Tkaczuk J" first="Jean" last="Tkaczuk">Jean Tkaczuk</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Czech, Kimberly A" sort="Czech, Kimberly A" uniqKey="Czech K" first="Kimberly A." last="Czech">Kimberly A. Czech</name><affiliation><mods:affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</mods:affiliation></affiliation></author><author><name sortKey="Tafani, Mathieu" sort="Tafani, Mathieu" uniqKey="Tafani M" first="Mathieu" last="Tafani">Mathieu Tafani</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Bastide, Raymond" sort="Bastide, Raymond" uniqKey="Bastide R" first="Raymond" last="Bastide">Raymond Bastide</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Caratero, Claude" sort="Caratero, Claude" uniqKey="Caratero C" first="Claude" last="Caratero">Claude Caratero</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation></author><author><name sortKey="Pappas, George D" sort="Pappas, George D" uniqKey="Pappas G" first="George D." last="Pappas">George D. Pappas</name><affiliation><mods:affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</mods:affiliation></affiliation></author><author><name sortKey="Lazorthes, Yves" sort="Lazorthes, Yves" uniqKey="Lazorthes Y" first="Yves" last="Lazorthes">Yves Lazorthes</name><affiliation><mods:affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Yves Lazorthes, M.D., Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse 31062, France.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Transplantation</title><title level="j" type="abbrev">CTR</title><idno type="ISSN">0963-6897</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="227">227</biblScope><biblScope unit="page" to="238">238</biblScope></imprint><idno type="ISSN">0963-6897</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0963-6897</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adrenal medulla</term><term>Cancer pain</term><term>Chromaffin cells</term><term>Neural transplants</term><term>Opioid peptides</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord [14, 19, 36]. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>chromaffin</json:string><json:string>medullary</json:string><json:string>transplantation</json:string><json:string>chromaffin cells</json:string><json:string>morphine</json:string><json:string>intrathecal</json:string><json:string>sagen</json:string><json:string>graft</json:string><json:string>spinal</json:string><json:string>allograft</json:string><json:string>pain scores</json:string><json:string>catecholamine</json:string><json:string>adrenal</json:string><json:string>subarachnoid</json:string><json:string>opioid</json:string><json:string>spinal cord</json:string><json:string>pain relief</json:string><json:string>adrenal medullary tissue</json:string><json:string>chronic pain</json:string><json:string>pappa</json:string><json:string>grafted</json:string><json:string>cancer pain</json:string><json:string>transplant</json:string><json:string>morphine intake</json:string><json:string>implant</json:string><json:string>cell transplantation</json:string><json:string>cyclosporin</json:string><json:string>subarachnoid space</json:string><json:string>xenogeneic</json:string><json:string>cancer patients</json:string><json:string>adrenal medullary transplants</json:string><json:string>second graft</json:string><json:string>opioid peptides</json:string><json:string>second transplant</json:string><json:string>peptide</json:string><json:string>clinical trials</json:string><json:string>lumbar cistern</json:string><json:string>intrathecal morphine</json:string><json:string>karnofsky index</json:string><json:string>adrenal medullary implants</json:string><json:string>chromaffin cell allograft</json:string><json:string>general condition</json:string><json:string>pain sensitivity</json:string><json:string>chromaffin cell implants</json:string><json:string>grafted tissue</json:string><json:string>adrenal medullary tissue pieces</json:string><json:string>first transplant</json:string><json:string>medullary tissue</json:string><json:string>cauda equina</json:string><json:string>lumbar</json:string><json:string>grafted tissue fragments</json:string><json:string>cell transplant</json:string><json:string>analgesic effect</json:string><json:string>spinal subarachnoid space</json:string><json:string>nodular formations</json:string><json:string>tyrosine hydroxylase</json:string><json:string>graft function</json:string><json:string>dura mater</json:string><json:string>oral morphine</json:string><json:string>intrathecal administration</json:string><json:string>growth factors</json:string><json:string>cortical tissue</json:string><json:string>autopsy specimens</json:string><json:string>adrenal chromaffin cells</json:string><json:string>access port</json:string><json:string>tissue fragments</json:string><json:string>multilobulated nodules</json:string><json:string>first week</json:string><json:string>fibroblast growth factor</json:string><json:string>balanced salt solution</json:string><json:string>graft tissue</json:string><json:string>immunosuppressive treatment</json:string><json:string>first patient</json:string><json:string>severe pain</json:string><json:string>standard conditions</json:string><json:string>adrenal medullary transplantation</json:string><json:string>tissue sections</json:string><json:string>alcohol solutions</json:string><json:string>toulouse university hospital</json:string><json:string>xenogeneic chromaffin cells</json:string><json:string>side effects</json:string><json:string>pretransplant levels</json:string><json:string>xenogeneic sources</json:string><json:string>chronic cancer pain</json:string><json:string>adrenal medulla</json:string><json:string>terminal period</json:string><json:string>morphine tolerance</json:string><json:string>morphine doses</json:string><json:string>culture media</json:string><json:string>dorsal horn</json:string><json:string>access ports</json:string><json:string>terminal cancer patients</json:string><json:string>irregular size</json:string><json:string>radicular sheath</json:string><json:string>nodular location</json:string><json:string>conus medullaris</json:string><json:string>animal studies</json:string><json:string>adrenal chromaffin cell implants</json:string><json:string>neuroactive substances</json:string><json:string>recent studies</json:string><json:string>transplant function</json:string><json:string>high correlation</json:string><json:string>patient pain scores</json:string><json:string>neurotrophic factors</json:string><json:string>peripheral neuropathy</json:string><json:string>university paul sabatier</json:string><json:string>cell therapy</json:string><json:string>elsevier science</json:string><json:string>intractable cancer pain</json:string></teeft></keywords><author><json:item><name>Jean C. Bés</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string></affiliations></json:item><json:item><name>Jean Tkaczuk</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string></affiliations></json:item><json:item><name>Kimberly A. Czech</name><affiliations><json:string>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</json:string></affiliations></json:item><json:item><name>Mathieu Tafani</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string></affiliations></json:item><json:item><name>Raymond Bastide</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string></affiliations></json:item><json:item><name>Claude Caratero</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string></affiliations></json:item><json:item><name>George D. Pappas</name><affiliations><json:string>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</json:string></affiliations></json:item><json:item><name>Yves Lazorthes</name><affiliations><json:string>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</json:string><json:string>Yves Lazorthes, M.D., Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse 31062, France.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Neural transplants</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Adrenal medulla</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chromaffin cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cancer pain</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Opioid peptides</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord [14, 19, 36]. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.</abstract><qualityIndicators><score>6.956</score><pdfVersion>1.2</pdfVersion><pdfPageSize>596 x 795 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1126</abstractCharCount><pdfWordCount>6264</pdfWordCount><pdfCharCount>40502</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>163</abstractWordCount></qualityIndicators><title>One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</title><pii><json:string>S0963-6897(97)00161-9</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Advances and technical standards in neurosurgery</title><language><json:string>unknown</json:string></language><volume>vol. 18</volume><pages><first>143</first><last>192</last></pages></serie><host><title>Cell Transplantation</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><issn><json:string>0963-6897</json:string></issn><pii><json:string>S0963-6897(00)X0023-1</json:string></pii><volume>7</volume><issue>3</issue><pages><first>227</first><last>238</last></pages><genre><json:string>journal</json:string></genre></host><categories><wos><json:string>science</json:string><json:string>transplantation</json:string><json:string>medicine, research & experimental</json:string><json:string>cell & tissue engineering</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string></inist></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0963-6897(97)00161-9</json:string></doi><id>2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1998 Elsevier Science Inc.</p></availability><date>1998</date></publicationStmt><notesStmt><note type="content">Section title: Original Contributions</note><note type="content">Fig. 1: (A) Patient-reported pain score (from 0 = no pain to 10 = the most intense pain). P1: patient 1; P2: patient 2; Single arrow = initial chromaffin cell graft; double arrows = second transplant. (B) The complementary daily intrathecal morphine intake (mg/24 h). (C) The Met-enkephalin release into the CSF (pg/mL). The mean basal level of CSF Met-enkephalin was done for 10 nongrafted patients (hatched areas). This was the reference value for the CSF Met-enkephalin rate of grafted patients.</note><note type="content">Fig. 2: Autopsy specimens from P1 show the dispersion of grafted tissue fragments (A, whole spinal cord, reduced, ruler length 16 cm) from the injections site (L4/L5). Note (arrows), multilobulated nodules of irregular size (B), clusters of adrenal medullary tissue between the roots of the cauda equina (C), and one nodular location in the inner aspect of the dura mater (D) (mag: B, 3×; C, 4×; D, 4×).</note><note type="content">Fig. 3: Low-power photomicrograph (A) of a section through two fragments of adrenal medullary tissue isolated from the lumbar-sacral roots and immunostained for chromagranin A (CGA) reactivity. At higher magnification (B), CGA-positive remnants revealed the rounded morphology of chromaffin cells and cellular and collagenous elements (arrowheads) between these nodular formations (mag: A, 320×; B, 2200×).</note><note type="content">Fig. 4: Low-power photomicrograph (A) of dopamine-β-hydroxylase (DβH)-stained section through the graft site. (B) Chromaffin cells immunohistochemically stained with tyrosine hydroxylase (TH) antibody with a rhodamine-linked secondary antibody marker. Note the intensity of TH labeling. (mag: A, 160×: B, 1200×).</note><note type="content">Fig. 5: The electron microscopy study (×30,000) show an important tissue alteration with degenerative chromaffin cells in necrotic zone but also the evidence of some secretory granuli.</note><note type="content">Table 1: The complementary intrathecal morphine intake (mg/24 h) and the CSF Met-enkephalin release (pg/mL) data</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</title><author xml:id="author-0000"><persName><forename type="first">Jean C.</forename><surname>Bés</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jean</forename><surname>Tkaczuk</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Kimberly A.</forename><surname>Czech</surname></persName><affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Mathieu</forename><surname>Tafani</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Raymond</forename><surname>Bastide</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Claude</forename><surname>Caratero</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation></author><author xml:id="author-0006"><persName><forename type="first">George D.</forename><surname>Pappas</surname></persName><affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Yves</forename><surname>Lazorthes</surname></persName><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><affiliation>Yves Lazorthes, M.D., Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse 31062, France.</affiliation></author><idno type="istex">2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A</idno><idno type="DOI">10.1016/S0963-6897(97)00161-9</idno><idno type="PII">S0963-6897(97)00161-9</idno></analytic><monogr><title level="j">Cell Transplantation</title><title level="j" type="abbrev">CTR</title><idno type="pISSN">0963-6897</idno><idno type="PII">S0963-6897(00)X0023-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">7</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="227">227</biblScope><biblScope unit="page" to="238">238</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord [14, 19, 36]. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Neural transplants</term></item><item><term>Adrenal medulla</term></item><item><term>Chromaffin cells</term></item><item><term>Cancer pain</term></item><item><term>Opioid peptides</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>CTR</jid><aid>5124</aid><ce:pii>S0963-6897(97)00161-9</ce:pii><ce:doi>10.1016/S0963-6897(97)00161-9</ce:doi><ce:copyright year="1998" type="full-transfer">Elsevier Science Inc.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Original Contributions</ce:textfn></ce:dochead><ce:title>One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</ce:title><ce:author-group><ce:author><ce:indexed-name>Bes</ce:indexed-name><ce:given-name>Jean C.</ce:given-name><ce:surname>Bés</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Tkaczuk</ce:indexed-name><ce:given-name>Jean</ce:given-name><ce:surname>Tkaczuk</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Czech</ce:indexed-name><ce:given-name>Kimberly A.</ce:given-name><ce:surname>Czech</ce:surname><ce:cross-ref refid="AFF2">B</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Tafani</ce:indexed-name><ce:given-name>Mathieu</ce:given-name><ce:surname>Tafani</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Bastide</ce:indexed-name><ce:given-name>Raymond</ce:given-name><ce:surname>Bastide</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Caratero</ce:indexed-name><ce:given-name>Claude</ce:given-name><ce:surname>Caratero</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Pappas</ce:indexed-name><ce:given-name>George D.</ce:given-name><ce:surname>Pappas</ce:surname><ce:cross-ref refid="AFF2">B</ce:cross-ref></ce:author><ce:author><ce:indexed-name>Lazorthes</ce:indexed-name><ce:given-name>Yves</ce:given-name><ce:surname>Lazorthes</ce:surname><ce:cross-ref refid="AFF1">A</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>A</ce:label><ce:textfn>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>B</ce:label><ce:textfn>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Yves Lazorthes, M.D., Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse 31062, France.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="26" month="11" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord <ce:cross-refs refid="BIB14 BIB19 BIB36">[14, 19, 36]</ce:cross-refs>. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Neural transplants</ce:text></ce:keyword><ce:keyword><ce:text>Adrenal medulla</ce:text></ce:keyword><ce:keyword><ce:text>Chromaffin cells</ce:text></ce:keyword><ce:keyword><ce:text>Cancer pain</ce:text></ce:keyword><ce:keyword><ce:text>Opioid peptides</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>One-Year Chromaffin Cell Allograft Survival in Cancer Patients With Chronic Pain: Morphological and Functional Evidence</title></titleInfo><name type="personal"><namePart type="given">Jean C.</namePart><namePart type="family">Bés</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Tkaczuk</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kimberly A.</namePart><namePart type="family">Czech</namePart><affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mathieu</namePart><namePart type="family">Tafani</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Raymond</namePart><namePart type="family">Bastide</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Claude</namePart><namePart type="family">Caratero</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George D.</namePart><namePart type="family">Pappas</namePart><affiliation>Department of Anatomy & Cell Biology, University of Illinois at Chicago, Chicago, IL, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yves</namePart><namePart type="family">Lazorthes</namePart><affiliation>Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse, 31062, France</affiliation><affiliation>Yves Lazorthes, M.D., Laboratory of Pain and Cell Therapy, Faculty of Medicine, University Paul Sabatier, 133 route de Narbonne, Toulouse 31062, France.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The control of chronic pain through transplantation of chromaffin cells has been reported over the past few years. Analgesic effects are principally due to the production of opioid peptides and catecholamines by chromaffin cells. Clinical trials have been reported with allografts consisting of whole-tissue fragments implanted into the subarachnoid space of the lumbar spinal cord [14, 19, 36]. In the present study, allogeneic grafts were successfully used to control chronic pain in two patients over a period of 1 yr based on patient reported pain scores, morphine intake, and CSF levels of Met-enkephalin. Macroscopic examination at autopsy located the transplanted tissue fragments in the form of multilobulated nodules at the level of the spinal axis and cauda equina. Immunocytochemical microscopy showed neuroendocrine cells are positive for chromagranin A (CGA), and enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DβH). The results suggest that there is a relationship between analgesic effect, Met-enkephalin levels in CSF, and the presence of chromaffin cells surviving in spinal subarachnoid space.</abstract><note type="content">Section title: Original Contributions</note><note type="content">Fig. 1: (A) Patient-reported pain score (from 0 = no pain to 10 = the most intense pain). P1: patient 1; P2: patient 2; Single arrow = initial chromaffin cell graft; double arrows = second transplant. (B) The complementary daily intrathecal morphine intake (mg/24 h). (C) The Met-enkephalin release into the CSF (pg/mL). The mean basal level of CSF Met-enkephalin was done for 10 nongrafted patients (hatched areas). This was the reference value for the CSF Met-enkephalin rate of grafted patients.</note><note type="content">Fig. 2: Autopsy specimens from P1 show the dispersion of grafted tissue fragments (A, whole spinal cord, reduced, ruler length 16 cm) from the injections site (L4/L5). Note (arrows), multilobulated nodules of irregular size (B), clusters of adrenal medullary tissue between the roots of the cauda equina (C), and one nodular location in the inner aspect of the dura mater (D) (mag: B, 3×; C, 4×; D, 4×).</note><note type="content">Fig. 3: Low-power photomicrograph (A) of a section through two fragments of adrenal medullary tissue isolated from the lumbar-sacral roots and immunostained for chromagranin A (CGA) reactivity. At higher magnification (B), CGA-positive remnants revealed the rounded morphology of chromaffin cells and cellular and collagenous elements (arrowheads) between these nodular formations (mag: A, 320×; B, 2200×).</note><note type="content">Fig. 4: Low-power photomicrograph (A) of dopamine-β-hydroxylase (DβH)-stained section through the graft site. (B) Chromaffin cells immunohistochemically stained with tyrosine hydroxylase (TH) antibody with a rhodamine-linked secondary antibody marker. Note the intensity of TH labeling. (mag: A, 160×: B, 1200×).</note><note type="content">Fig. 5: The electron microscopy study (×30,000) show an important tissue alteration with degenerative chromaffin cells in necrotic zone but also the evidence of some secretory granuli.</note><note type="content">Table 1: The complementary intrathecal morphine intake (mg/24 h) and the CSF Met-enkephalin release (pg/mL) data</note><subject lang="en"><genre>Keywords</genre><topic>Neural transplants</topic><topic>Adrenal medulla</topic><topic>Chromaffin cells</topic><topic>Cancer pain</topic><topic>Opioid peptides</topic></subject><relatedItem type="host"><titleInfo><title>Cell Transplantation</title></titleInfo><titleInfo type="abbreviated"><title>CTR</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199805</dateIssued></originInfo><identifier type="ISSN">0963-6897</identifier><identifier type="PII">S0963-6897(00)X0023-1</identifier><part><date>199805</date><detail type="volume"><number>7</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue pages"><start>227</start><end>338</end></extent><extent unit="pages"><start>227</start><end>238</end></extent></part></relatedItem><identifier type="istex">2205CC8D5803B03DB7C4E16B9CF7D1CAE66F387A</identifier><identifier type="DOI">10.1016/S0963-6897(97)00161-9</identifier><identifier type="PII">S0963-6897(97)00161-9</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©1998</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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