Methods for Lymphatic Vessel Culture and Gene Transfection
Identifieur interne : 000D40 ( Istex/Checkpoint ); précédent : 000D39; suivant : 000D41Methods for Lymphatic Vessel Culture and Gene Transfection
Auteurs : Anatoliy A. Gashev [États-Unis] ; Michael J. Davis [États-Unis] ; Olga Y. Gasheva [États-Unis] ; Zhanna V. Nepiushchikh [États-Unis] ; Wei Wang [États-Unis] ; Patrick Dougherty [États-Unis] ; Katherine A. Kelly [États-Unis] ; Shijie Cai [Royaume-Uni] ; Pierre-Yves Von Der Weid [Canada] ; Mariappan Muthuchamy [États-Unis] ; Cynthia J. Meininger [États-Unis] ; David C. Zawieja [États-Unis]Source :
- Microcirculation [ 1073-9688 ] ; 2009-10.
Abstract
Objective: To develop the techniques needed for the specific gene/protein targeting transfection experiments in isolated lymphatic vessels, we completed two major tasks: 1) optimize the experimental conditions to maintain the viability of isolated rat lymphatic vessels in culture for sufficiently long periods of time to permit knockdown or overexpression of selected proteins/genes and 2) develop effective transfection protocols for lymphatic muscle and endothelial cells in intact lymphatic vessels without nonspecific impairment of lymphatic contractile function due to the transfection protocol itself.
Url:
DOI: 10.1080/10739680903120778
Affiliations:
- Canada, Royaume-Uni, États-Unis
- Alberta, Angleterre, Missouri (État), Oxfordshire, Texas
- Calgary, Oxford
- Université d'Oxford, Université de Calgary
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:BC921AAC6920745A79CA8C190366EBDA830393EALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Methods for Lymphatic Vessel Culture and Gene Transfection</title>
<author><name sortKey="Gashev, Anatoliy A" sort="Gashev, Anatoliy A" uniqKey="Gashev A" first="Anatoliy A." last="Gashev">Anatoliy A. Gashev</name>
</author>
<author><name sortKey="Davis, Michael J" sort="Davis, Michael J" uniqKey="Davis M" first="Michael J." last="Davis">Michael J. Davis</name>
</author>
<author><name sortKey="Gasheva, Olga Y" sort="Gasheva, Olga Y" uniqKey="Gasheva O" first="Olga Y." last="Gasheva">Olga Y. Gasheva</name>
</author>
<author><name sortKey="Nepiushchikh, Zhanna V" sort="Nepiushchikh, Zhanna V" uniqKey="Nepiushchikh Z" first="Zhanna V." last="Nepiushchikh">Zhanna V. Nepiushchikh</name>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
</author>
<author><name sortKey="Dougherty, Patrick" sort="Dougherty, Patrick" uniqKey="Dougherty P" first="Patrick" last="Dougherty">Patrick Dougherty</name>
</author>
<author><name sortKey="Kelly, Katherine A" sort="Kelly, Katherine A" uniqKey="Kelly K" first="Katherine A." last="Kelly">Katherine A. Kelly</name>
</author>
<author><name sortKey="Cai, Shijie" sort="Cai, Shijie" uniqKey="Cai S" first="Shijie" last="Cai">Shijie Cai</name>
</author>
<author><name sortKey="Von Der Weid, Pierre Ves" sort="Von Der Weid, Pierre Ves" uniqKey="Von Der Weid P" first="Pierre-Yves" last="Von Der Weid">Pierre-Yves Von Der Weid</name>
</author>
<author><name sortKey="Muthuchamy, Mariappan" sort="Muthuchamy, Mariappan" uniqKey="Muthuchamy M" first="Mariappan" last="Muthuchamy">Mariappan Muthuchamy</name>
</author>
<author><name sortKey="Meininger, Cynthia J" sort="Meininger, Cynthia J" uniqKey="Meininger C" first="Cynthia J." last="Meininger">Cynthia J. Meininger</name>
</author>
<author><name sortKey="Zawieja, David C" sort="Zawieja, David C" uniqKey="Zawieja D" first="David C." last="Zawieja">David C. Zawieja</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:BC921AAC6920745A79CA8C190366EBDA830393EA</idno>
<date when="2009" year="2009">2009</date>
<idno type="doi">10.1080/10739680903120778</idno>
<idno type="url">https://api.istex.fr/document/BC921AAC6920745A79CA8C190366EBDA830393EA/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">005874</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">005874</idno>
<idno type="wicri:Area/Istex/Curation">005874</idno>
<idno type="wicri:Area/Istex/Checkpoint">000D40</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000D40</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Methods for Lymphatic Vessel Culture and Gene Transfection</title>
<author><name sortKey="Gashev, Anatoliy A" sort="Gashev, Anatoliy A" uniqKey="Gashev A" first="Anatoliy A." last="Gashev">Anatoliy A. Gashev</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Davis, Michael J" sort="Davis, Michael J" uniqKey="Davis M" first="Michael J." last="Davis">Michael J. Davis</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Medical Pharmacology & Physiology, University of Missouri, Columbia, Missouri</wicri:regionArea>
<placeName><region type="state">Missouri (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gasheva, Olga Y" sort="Gasheva, Olga Y" uniqKey="Gasheva O" first="Olga Y." last="Gasheva">Olga Y. Gasheva</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nepiushchikh, Zhanna V" sort="Nepiushchikh, Zhanna V" uniqKey="Nepiushchikh Z" first="Zhanna V." last="Nepiushchikh">Zhanna V. Nepiushchikh</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dougherty, Patrick" sort="Dougherty, Patrick" uniqKey="Dougherty P" first="Patrick" last="Dougherty">Patrick Dougherty</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kelly, Katherine A" sort="Kelly, Katherine A" uniqKey="Kelly K" first="Katherine A." last="Kelly">Katherine A. Kelly</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cai, Shijie" sort="Cai, Shijie" uniqKey="Cai S" first="Shijie" last="Cai">Shijie Cai</name>
<affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Clinical Pharmacology, University of Oxford, Oxford</wicri:regionArea>
<placeName><settlement type="city">Oxford</settlement>
<region type="country">Angleterre</region>
<region type="comté" nuts="2">Oxfordshire</region>
<settlement type="city">Oxford</settlement>
</placeName>
<orgName type="university">Université d'Oxford</orgName>
</affiliation>
</author>
<author><name sortKey="Von Der Weid, Pierre Ves" sort="Von Der Weid, Pierre Ves" uniqKey="Von Der Weid P" first="Pierre-Yves" last="Von Der Weid">Pierre-Yves Von Der Weid</name>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta</wicri:regionArea>
<orgName type="university">Université de Calgary</orgName>
<placeName><settlement type="city">Calgary</settlement>
<region type="state">Alberta</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Muthuchamy, Mariappan" sort="Muthuchamy, Mariappan" uniqKey="Muthuchamy M" first="Mariappan" last="Muthuchamy">Mariappan Muthuchamy</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Meininger, Cynthia J" sort="Meininger, Cynthia J" uniqKey="Meininger C" first="Cynthia J." last="Meininger">Cynthia J. Meininger</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zawieja, David C" sort="Zawieja, David C" uniqKey="Zawieja D" first="David C." last="Zawieja">David C. Zawieja</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Division of Lymphatic Biology, Texas A&M Health Science Center, Temple, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Microcirculation</title>
<title level="j" type="alt">MICROCIRCULATION</title>
<idno type="ISSN">1073-9688</idno>
<idno type="eISSN">1549-8719</idno>
<imprint><biblScope unit="vol">16</biblScope>
<biblScope unit="issue">7</biblScope>
<biblScope unit="page" from="615">615</biblScope>
<biblScope unit="page" to="628">628</biblScope>
<biblScope unit="page-count">14</biblScope>
<publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="2009-10">2009-10</date>
</imprint>
<idno type="ISSN">1073-9688</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1073-9688</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Objective: To develop the techniques needed for the specific gene/protein targeting transfection experiments in isolated lymphatic vessels, we completed two major tasks: 1) optimize the experimental conditions to maintain the viability of isolated rat lymphatic vessels in culture for sufficiently long periods of time to permit knockdown or overexpression of selected proteins/genes and 2) develop effective transfection protocols for lymphatic muscle and endothelial cells in intact lymphatic vessels without nonspecific impairment of lymphatic contractile function due to the transfection protocol itself.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
<region><li>Alberta</li>
<li>Angleterre</li>
<li>Missouri (État)</li>
<li>Oxfordshire</li>
<li>Texas</li>
</region>
<settlement><li>Calgary</li>
<li>Oxford</li>
</settlement>
<orgName><li>Université d'Oxford</li>
<li>Université de Calgary</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Texas"><name sortKey="Gashev, Anatoliy A" sort="Gashev, Anatoliy A" uniqKey="Gashev A" first="Anatoliy A." last="Gashev">Anatoliy A. Gashev</name>
</region>
<name sortKey="Davis, Michael J" sort="Davis, Michael J" uniqKey="Davis M" first="Michael J." last="Davis">Michael J. Davis</name>
<name sortKey="Dougherty, Patrick" sort="Dougherty, Patrick" uniqKey="Dougherty P" first="Patrick" last="Dougherty">Patrick Dougherty</name>
<name sortKey="Gashev, Anatoliy A" sort="Gashev, Anatoliy A" uniqKey="Gashev A" first="Anatoliy A." last="Gashev">Anatoliy A. Gashev</name>
<name sortKey="Gasheva, Olga Y" sort="Gasheva, Olga Y" uniqKey="Gasheva O" first="Olga Y." last="Gasheva">Olga Y. Gasheva</name>
<name sortKey="Kelly, Katherine A" sort="Kelly, Katherine A" uniqKey="Kelly K" first="Katherine A." last="Kelly">Katherine A. Kelly</name>
<name sortKey="Meininger, Cynthia J" sort="Meininger, Cynthia J" uniqKey="Meininger C" first="Cynthia J." last="Meininger">Cynthia J. Meininger</name>
<name sortKey="Muthuchamy, Mariappan" sort="Muthuchamy, Mariappan" uniqKey="Muthuchamy M" first="Mariappan" last="Muthuchamy">Mariappan Muthuchamy</name>
<name sortKey="Nepiushchikh, Zhanna V" sort="Nepiushchikh, Zhanna V" uniqKey="Nepiushchikh Z" first="Zhanna V." last="Nepiushchikh">Zhanna V. Nepiushchikh</name>
<name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<name sortKey="Zawieja, David C" sort="Zawieja, David C" uniqKey="Zawieja D" first="David C." last="Zawieja">David C. Zawieja</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Cai, Shijie" sort="Cai, Shijie" uniqKey="Cai S" first="Shijie" last="Cai">Shijie Cai</name>
</region>
</country>
<country name="Canada"><region name="Alberta"><name sortKey="Von Der Weid, Pierre Ves" sort="Von Der Weid, Pierre Ves" uniqKey="Von Der Weid P" first="Pierre-Yves" last="Von Der Weid">Pierre-Yves Von Der Weid</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000D40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Istex |étape= Checkpoint |type= RBID |clé= ISTEX:BC921AAC6920745A79CA8C190366EBDA830393EA |texte= Methods for Lymphatic Vessel Culture and Gene Transfection }}
This area was generated with Dilib version V0.6.31. |