Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.
Identifieur interne : 000439 ( PubMed/Corpus ); précédent : 000438; suivant : 000440Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.
Auteurs : S Q Li ; J L Schulman ; T. Moran ; C. Bona ; P. PaleseSource :
- Journal of virology [ 0022-538X ] ; 1992.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (immunology), Base Sequence, Cell Line, Chimera, DNA, Viral, Epitopes (genetics), Epitopes (immunology), Hemagglutinin Glycoproteins, Influenza Virus, Hemagglutinins, Viral (genetics), Hemagglutinins, Viral (immunology), Influenza A virus (genetics), Influenza A virus (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutagenesis, Site-Directed, Transfection.
- MESH :
- chemical , genetics : Epitopes, Hemagglutinins, Viral.
- chemical , immunology : Antibodies, Viral, Epitopes, Hemagglutinins, Viral.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chimera, DNA, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutagenesis, Site-Directed, Transfection.
Abstract
Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.
PubMed: 1370088
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Palese</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1370088</idno><idno type="pmid">1370088</idno><idno type="wicri:Area/PubMed/Corpus">000439</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000439</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.</title><author><name sortKey="Li, S Q" sort="Li, S Q" uniqKey="Li S" first="S Q" last="Li">S Q Li</name><affiliation><nlm:affiliation>Department of Microbiology, Mt. Sinai School of Medicine, New York, New York 10029.</nlm:affiliation></affiliation></author><author><name sortKey="Schulman, J L" sort="Schulman, J L" uniqKey="Schulman J" first="J L" last="Schulman">J L Schulman</name></author><author><name sortKey="Moran, T" sort="Moran, T" uniqKey="Moran T" first="T" last="Moran">T. Moran</name></author><author><name sortKey="Bona, C" sort="Bona, C" uniqKey="Bona C" first="C" last="Bona">C. Bona</name></author><author><name sortKey="Palese, P" sort="Palese, P" uniqKey="Palese P" first="P" last="Palese">P. Palese</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Base Sequence</term><term>Cell Line</term><term>Chimera</term><term>DNA, Viral</term><term>Epitopes (genetics)</term><term>Epitopes (immunology)</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Hemagglutinins, Viral (genetics)</term><term>Hemagglutinins, Viral (immunology)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Transfection</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Epitopes</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Chimera</term><term>DNA, Viral</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1370088</PMID><DateCompleted><Year>1992</Year><Month>01</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>66</Volume><Issue>1</Issue><PubDate><Year>1992</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.</ArticleTitle><Pagination><MedlinePgn>399-404</MedlinePgn></Pagination><Abstract><AbstractText>Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>S Q</ForeName><Initials>SQ</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Mt. Sinai School of Medicine, New York, New York 10029.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schulman</LastName><ForeName>J L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Moran</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Bona</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Palese</LastName><ForeName>P</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>AI 24460</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J 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MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002678" MajorTopicYN="N">Chimera</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006389" MajorTopicYN="N">Hemagglutinins, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName></MeshHeading><MeshHeading><DescriptorName 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