Infection of human retinal pigment epithelial cells with influenza A viruses.
Identifieur interne : 000228 ( PubMed/Corpus ); précédent : 000227; suivant : 000229Infection of human retinal pigment epithelial cells with influenza A viruses.
Auteurs : Martin Michaelis ; Janina Geiler ; Denise Klassert ; Hans Wilhelm Doerr ; Jindrich CinatlSource :
- Investigative ophthalmology & visual science [ 1552-5783 ] ; 2009.
English descriptors
- KwdEn :
- Antiviral Agents (pharmacology), Apoptosis, Caspase 3 (metabolism), Cells, Cultured, Chemokines (metabolism), Cytopathogenic Effect, Viral, Dose-Response Relationship, Drug, Humans, Influenza A Virus, H1N1 Subtype (physiology), Influenza A Virus, H2N2 Subtype (physiology), Influenza A Virus, H5N1 Subtype (physiology), Interferon Type I (pharmacology), Interferon-gamma (pharmacology), RNA, Messenger (metabolism), Retinal Pigment Epithelium (pathology), Retinal Pigment Epithelium (virology), Reverse Transcriptase Polymerase Chain Reaction, Ribavirin (pharmacology), Virus Replication (drug effects), Virus Replication (physiology).
- MESH :
- chemical , metabolism : Caspase 3, Chemokines, RNA, Messenger.
- chemical , pharmacology : Antiviral Agents, Interferon Type I, Interferon-gamma, Ribavirin.
- drug effects : Virus Replication.
- pathology : Retinal Pigment Epithelium.
- physiology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H5N1 Subtype, Virus Replication.
- virology : Retinal Pigment Epithelium.
- Apoptosis, Cells, Cultured, Cytopathogenic Effect, Viral, Dose-Response Relationship, Drug, Humans, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Ocular involvement in influenza A virus diseases is common but usually limited to mild conjunctivitis. Rarely, inflammation of the choriocapillaris may result in atrophia of the retinal pigment epithelium (RPE). Primary human retinal pigment epithelial (RPE) cells were infected with seasonal (H1N1 A/New Caledonia/20/99, H3N2 A/California/7/2004) or highly pathogenic avian H5N1 (A/Thailand/1(Kan-1)/04, A/Vietnam/1203/04, A/Vietnam/1194/04) influenza strains.
DOI: 10.1167/iovs.09-3752
PubMed: 19553611
Links to Exploration step
pubmed:19553611Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Infection of human retinal pigment epithelial cells with influenza A viruses.</title>
<author><name sortKey="Michaelis, Martin" sort="Michaelis, Martin" uniqKey="Michaelis M" first="Martin" last="Michaelis">Martin Michaelis</name>
<affiliation><nlm:affiliation>Institut für Medizinische Virologie, Klinikum der J. W. Goethe-Universität, Frankfurt-am-Main, Germany.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Geiler, Janina" sort="Geiler, Janina" uniqKey="Geiler J" first="Janina" last="Geiler">Janina Geiler</name>
</author>
<author><name sortKey="Klassert, Denise" sort="Klassert, Denise" uniqKey="Klassert D" first="Denise" last="Klassert">Denise Klassert</name>
</author>
<author><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name>
</author>
<author><name sortKey="Cinatl, Jindrich" sort="Cinatl, Jindrich" uniqKey="Cinatl J" first="Jindrich" last="Cinatl">Jindrich Cinatl</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Infection of human retinal pigment epithelial cells with influenza A viruses.</title>
<author><name sortKey="Michaelis, Martin" sort="Michaelis, Martin" uniqKey="Michaelis M" first="Martin" last="Michaelis">Martin Michaelis</name>
<affiliation><nlm:affiliation>Institut für Medizinische Virologie, Klinikum der J. W. Goethe-Universität, Frankfurt-am-Main, Germany.</nlm:affiliation>
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<author><name sortKey="Geiler, Janina" sort="Geiler, Janina" uniqKey="Geiler J" first="Janina" last="Geiler">Janina Geiler</name>
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<author><name sortKey="Klassert, Denise" sort="Klassert, Denise" uniqKey="Klassert D" first="Denise" last="Klassert">Denise Klassert</name>
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<author><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name>
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<author><name sortKey="Cinatl, Jindrich" sort="Cinatl, Jindrich" uniqKey="Cinatl J" first="Jindrich" last="Cinatl">Jindrich Cinatl</name>
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<series><title level="j">Investigative ophthalmology & visual science</title>
<idno type="eISSN">1552-5783</idno>
<imprint><date when="2009" type="published">2009</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (pharmacology)</term>
<term>Apoptosis</term>
<term>Caspase 3 (metabolism)</term>
<term>Cells, Cultured</term>
<term>Chemokines (metabolism)</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype (physiology)</term>
<term>Influenza A Virus, H2N2 Subtype (physiology)</term>
<term>Influenza A Virus, H5N1 Subtype (physiology)</term>
<term>Interferon Type I (pharmacology)</term>
<term>Interferon-gamma (pharmacology)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Retinal Pigment Epithelium (pathology)</term>
<term>Retinal Pigment Epithelium (virology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Ribavirin (pharmacology)</term>
<term>Virus Replication (drug effects)</term>
<term>Virus Replication (physiology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspase 3</term>
<term>Chemokines</term>
<term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Interferon Type I</term>
<term>Interferon-gamma</term>
<term>Ribavirin</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Retinal Pigment Epithelium</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Retinal Pigment Epithelium</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Apoptosis</term>
<term>Cells, Cultured</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<front><div type="abstract" xml:lang="en">Ocular involvement in influenza A virus diseases is common but usually limited to mild conjunctivitis. Rarely, inflammation of the choriocapillaris may result in atrophia of the retinal pigment epithelium (RPE). Primary human retinal pigment epithelial (RPE) cells were infected with seasonal (H1N1 A/New Caledonia/20/99, H3N2 A/California/7/2004) or highly pathogenic avian H5N1 (A/Thailand/1(Kan-1)/04, A/Vietnam/1203/04, A/Vietnam/1194/04) influenza strains.</div>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19553611</PMID>
<DateCompleted><Year>2009</Year>
<Month>12</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1552-5783</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>50</Volume>
<Issue>11</Issue>
<PubDate><Year>2009</Year>
<Month>Nov</Month>
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<Title>Investigative ophthalmology & visual science</Title>
<ISOAbbreviation>Invest. Ophthalmol. Vis. Sci.</ISOAbbreviation>
</Journal>
<ArticleTitle>Infection of human retinal pigment epithelial cells with influenza A viruses.</ArticleTitle>
<Pagination><MedlinePgn>5419-25</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1167/iovs.09-3752</ELocationID>
<Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">Ocular involvement in influenza A virus diseases is common but usually limited to mild conjunctivitis. Rarely, inflammation of the choriocapillaris may result in atrophia of the retinal pigment epithelium (RPE). Primary human retinal pigment epithelial (RPE) cells were infected with seasonal (H1N1 A/New Caledonia/20/99, H3N2 A/California/7/2004) or highly pathogenic avian H5N1 (A/Thailand/1(Kan-1)/04, A/Vietnam/1203/04, A/Vietnam/1194/04) influenza strains.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Influenza A virus replication was studied by investigation of cytopathogenic effects, immune staining for influenza A virus nucleoprotein, determination of virus titers, and electron microscopy. Apoptosis induction was examined by immune staining for activated caspase 3 and cleaved PARP. Proinflammatory gene expression was investigated by quantitative PCR.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">H5N1 but not seasonal influenza strains replicated to high titers (>10(8) TCID(50)/mL; 50% tissue culture infectious dose/milliliter) in RPE cells. H5N1 infection resulted in RPE cell apoptosis that was abolished by the antiviral drug ribavirin. Pretreatment with type I interferons (interferon-alpha and -beta) or the type II interferon, (interferon-gamma), inhibited H5N1 replication. Moreover, H5N1 infection induced expression of proinflammatory genes (tumor necrosis factor-alpha, CXCL8, CXCL10, CXCL11, and interleukin-6), which was inhibited by ribavirin in a concentration-dependent manner.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">A novel cell type derived from the central nervous system was permissive to H5N1 influenza virus replication. This findings supports those suggesting H5N1 influenza strains to own a greater potential to spread to nonrespiratory tissues than seasonal human influenza viruses. Moreover, the data warrant the further study of the role of influenza A virus replication in retinal diseases associated with influenza A virus infections.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Michaelis</LastName>
<ForeName>Martin</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Institut für Medizinische Virologie, Klinikum der J. W. Goethe-Universität, Frankfurt-am-Main, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Geiler</LastName>
<ForeName>Janina</ForeName>
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</Author>
<Author ValidYN="Y"><LastName>Klassert</LastName>
<ForeName>Denise</ForeName>
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<Author ValidYN="Y"><LastName>Doerr</LastName>
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<Author ValidYN="Y"><LastName>Cinatl</LastName>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Invest Ophthalmol Vis Sci</MedlineTA>
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<MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName>
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