[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].
Identifieur interne : 000408 ( PubMed/Checkpoint ); précédent : 000407; suivant : 000409[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].
Auteurs : N P Arbatski ; A O Zheltova ; D V Iurtov ; V A Derevitskaia ; N K KochetkovSource :
- Bioorganicheskaia khimiia [ 0132-3423 ] ; 1989.
Descripteurs français
- KwdFr :
- Acides aminés (analyse), Chromatographie en phase liquide à haute performance, Glycoprotéines (analyse), Hémagglutinines virales (analyse), Oligosaccharides (analyse), Sialidase (analyse), Sous-type H2N2 du virus de la grippe A, Virus de la grippe A (analyse), Virus de la grippe A (enzymologie), Virus de la grippe A (immunologie).
- MESH :
- analyse : Acides aminés, Glycoprotéines, Hémagglutinines virales, Oligosaccharides, Sialidase, Virus de la grippe A.
- enzymologie : Virus de la grippe A.
- immunologie : Virus de la grippe A.
- Chromatographie en phase liquide à haute performance, Sous-type H2N2 du virus de la grippe A.
English descriptors
- KwdEn :
- Amino Acids (analysis), Chromatography, High Pressure Liquid, Glycoproteins (analysis), Hemagglutinins, Viral (analysis), Influenza A Virus, H2N2 Subtype, Influenza A virus (analysis), Influenza A virus (enzymology), Influenza A virus (immunology), Neuraminidase (analysis), Oligosaccharides (analysis).
- MESH :
- chemical , analysis : Amino Acids, Glycoproteins, Hemagglutinins, Viral, Neuraminidase, Oligosaccharides.
- analysis : Influenza A virus.
- enzymology : Influenza A virus.
- immunology : Influenza A virus.
- Chromatography, High Pressure Liquid, Influenza A Virus, H2N2 Subtype.
Abstract
The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.
PubMed: 2634965
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:2634965Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].</title>
<author><name sortKey="Arbatski, N P" sort="Arbatski, N P" uniqKey="Arbatski N" first="N P" last="Arbatski">N P Arbatski</name>
</author>
<author><name sortKey="Zheltova, A O" sort="Zheltova, A O" uniqKey="Zheltova A" first="A O" last="Zheltova">A O Zheltova</name>
</author>
<author><name sortKey="Iurtov, D V" sort="Iurtov, D V" uniqKey="Iurtov D" first="D V" last="Iurtov">D V Iurtov</name>
</author>
<author><name sortKey="Derevitskaia, V A" sort="Derevitskaia, V A" uniqKey="Derevitskaia V" first="V A" last="Derevitskaia">V A Derevitskaia</name>
</author>
<author><name sortKey="Kochetkov, N K" sort="Kochetkov, N K" uniqKey="Kochetkov N" first="N K" last="Kochetkov">N K Kochetkov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1989">1989</date>
<idno type="RBID">pubmed:2634965</idno>
<idno type="pmid">2634965</idno>
<idno type="wicri:Area/PubMed/Corpus">000457</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000457</idno>
<idno type="wicri:Area/PubMed/Curation">000457</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000457</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000408</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000408</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].</title>
<author><name sortKey="Arbatski, N P" sort="Arbatski, N P" uniqKey="Arbatski N" first="N P" last="Arbatski">N P Arbatski</name>
</author>
<author><name sortKey="Zheltova, A O" sort="Zheltova, A O" uniqKey="Zheltova A" first="A O" last="Zheltova">A O Zheltova</name>
</author>
<author><name sortKey="Iurtov, D V" sort="Iurtov, D V" uniqKey="Iurtov D" first="D V" last="Iurtov">D V Iurtov</name>
</author>
<author><name sortKey="Derevitskaia, V A" sort="Derevitskaia, V A" uniqKey="Derevitskaia V" first="V A" last="Derevitskaia">V A Derevitskaia</name>
</author>
<author><name sortKey="Kochetkov, N K" sort="Kochetkov, N K" uniqKey="Kochetkov N" first="N K" last="Kochetkov">N K Kochetkov</name>
</author>
</analytic>
<series><title level="j">Bioorganicheskaia khimiia</title>
<idno type="ISSN">0132-3423</idno>
<imprint><date when="1989" type="published">1989</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (analysis)</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Glycoproteins (analysis)</term>
<term>Hemagglutinins, Viral (analysis)</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A virus (analysis)</term>
<term>Influenza A virus (enzymology)</term>
<term>Influenza A virus (immunology)</term>
<term>Neuraminidase (analysis)</term>
<term>Oligosaccharides (analysis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (analyse)</term>
<term>Chromatographie en phase liquide à haute performance</term>
<term>Glycoprotéines (analyse)</term>
<term>Hémagglutinines virales (analyse)</term>
<term>Oligosaccharides (analyse)</term>
<term>Sialidase (analyse)</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Virus de la grippe A (analyse)</term>
<term>Virus de la grippe A (enzymologie)</term>
<term>Virus de la grippe A (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Amino Acids</term>
<term>Glycoproteins</term>
<term>Hemagglutinins, Viral</term>
<term>Neuraminidase</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Acides aminés</term>
<term>Glycoprotéines</term>
<term>Hémagglutinines virales</term>
<term>Oligosaccharides</term>
<term>Sialidase</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term>
<term>Influenza A Virus, H2N2 Subtype</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2634965</PMID>
<DateCompleted><Year>1990</Year>
<Month>06</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0132-3423</ISSN>
<JournalIssue CitedMedium="Print"><Volume>15</Volume>
<Issue>12</Issue>
<PubDate><Year>1989</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Bioorganicheskaia khimiia</Title>
<ISOAbbreviation>Bioorg. Khim.</ISOAbbreviation>
</Journal>
<ArticleTitle>[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].</ArticleTitle>
<Pagination><MedlinePgn>1641-8</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arbatskiĭ</LastName>
<ForeName>N P</ForeName>
<Initials>NP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zheltova</LastName>
<ForeName>A O</ForeName>
<Initials>AO</Initials>
</Author>
<Author ValidYN="Y"><LastName>Iurtov</LastName>
<ForeName>D V</ForeName>
<Initials>DV</Initials>
</Author>
<Author ValidYN="Y"><LastName>Derevitskaia</LastName>
<ForeName>V A</ForeName>
<Initials>VA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kochetkov</LastName>
<ForeName>N K</ForeName>
<Initials>NK</Initials>
</Author>
</AuthorList>
<Language>rus</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<VernacularTitle>Struktura oligosakharidnykh fragmentov glikoproteinov virusa grippa A/Krasnodar/101/59(H2N2)--tiazheloĭ i legkoĭ tsepeĭ gemaggliutinina i neĭraminidazy.</VernacularTitle>
</Article>
<MedlineJournalInfo><Country>Russia (Federation)</Country>
<MedlineTA>Bioorg Khim</MedlineTA>
<NlmUniqueID>7804941</NlmUniqueID>
<ISSNLinking>0132-3423</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006389">Hemagglutinins, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009844">Oligosaccharides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber>
<NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006389" MajorTopicYN="N">Hemagglutinins, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053121" MajorTopicYN="Y">Influenza A Virus, H2N2 Subtype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009844" MajorTopicYN="N">Oligosaccharides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1989</Year>
<Month>12</Month>
<Day>1</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1989</Year>
<Month>12</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1989</Year>
<Month>12</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">2634965</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list></list>
<tree><noCountry><name sortKey="Arbatski, N P" sort="Arbatski, N P" uniqKey="Arbatski N" first="N P" last="Arbatski">N P Arbatski</name>
<name sortKey="Derevitskaia, V A" sort="Derevitskaia, V A" uniqKey="Derevitskaia V" first="V A" last="Derevitskaia">V A Derevitskaia</name>
<name sortKey="Iurtov, D V" sort="Iurtov, D V" uniqKey="Iurtov D" first="D V" last="Iurtov">D V Iurtov</name>
<name sortKey="Kochetkov, N K" sort="Kochetkov, N K" uniqKey="Kochetkov N" first="N K" last="Kochetkov">N K Kochetkov</name>
<name sortKey="Zheltova, A O" sort="Zheltova, A O" uniqKey="Zheltova A" first="A O" last="Zheltova">A O Zheltova</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000408 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000408 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:2634965 |texte= [The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase]. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:2634965" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |