Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor
Identifieur interne : 000B74 ( Pmc/Curation ); précédent : 000B73; suivant : 000B75Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor
Auteurs : Subash C. B. Gopinath [Japon] ; Koichi Awazu [Japon] ; Makoto Fujimaki [Japon] ; Kazufumi Shimizu [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among infectious diseases, influenza viral infections are a worldwide cause of pandemic disease and mortality. With the emergence of different influenza strains, it is vital to develop a method using antibodies that can differentiate between viral types and subtypes. Ideally, such a system would also be user friendly. In this study, a polyclonal antibody generated against A/Udorn/307/1972 (H3N2) was used as a probe to distinguish between influenza H3N2 viruses based on the interaction between the antibody and hemagglutinin, demonstrating its applicability for viral discrimination. Clear discrimination was demonstrated using an evanescent-field-coupled waveguide-mode sensor, which has appealing characteristics over other methods in the viewpoint of improving the sensitivity, measurement time, portability and usability. Further supporting evidence was obtained using enzyme-linked immunosorbent assays, hemagglutination-inhibition assays, and infectivity neutralization assays. The results obtained indicate that the polyclonal antibody used here is a potential probe for distinguishing influenza viruses and, with the aid of a handheld sensor it could be used for influenza surveillance.
Url:
DOI: 10.1371/journal.pone.0081396
PubMed: 24339924
PubMed Central: 3858306
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor</title><author><name sortKey="Gopinath, Subash C B" sort="Gopinath, Subash C B" uniqKey="Gopinath S" first="Subash C. B." last="Gopinath">Subash C. B. Gopinath</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Awazu, Koichi" sort="Awazu, Koichi" uniqKey="Awazu K" first="Koichi" last="Awazu">Koichi Awazu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Fujimaki, Makoto" sort="Fujimaki, Makoto" uniqKey="Fujimaki M" first="Makoto" last="Fujimaki">Makoto Fujimaki</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Shimizu, Kazufumi" sort="Shimizu, Kazufumi" uniqKey="Shimizu K" first="Kazufumi" last="Shimizu">Kazufumi Shimizu</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Open Research Center for Genome and Infectious Disease Control, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Open Research Center for Genome and Infectious Disease Control, Nihon University School of Medicine, Itabashi-ku, Tokyo</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24339924</idno><idno type="pmc">3858306</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858306</idno><idno type="RBID">PMC:3858306</idno><idno type="doi">10.1371/journal.pone.0081396</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000B74</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B74</idno><idno type="wicri:Area/Pmc/Curation">000B74</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B74</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor</title><author><name sortKey="Gopinath, Subash C B" sort="Gopinath, Subash C B" uniqKey="Gopinath S" first="Subash C. B." last="Gopinath">Subash C. B. Gopinath</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Awazu, Koichi" sort="Awazu, Koichi" uniqKey="Awazu K" first="Koichi" last="Awazu">Koichi Awazu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Fujimaki, Makoto" sort="Fujimaki, Makoto" uniqKey="Fujimaki M" first="Makoto" last="Fujimaki">Makoto Fujimaki</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki</wicri:regionArea></affiliation></author><author><name sortKey="Shimizu, Kazufumi" sort="Shimizu, Kazufumi" uniqKey="Shimizu K" first="Kazufumi" last="Shimizu">Kazufumi Shimizu</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Open Research Center for Genome and Infectious Disease Control, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Open Research Center for Genome and Infectious Disease Control, Nihon University School of Medicine, Itabashi-ku, Tokyo</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among infectious diseases, influenza viral infections are a worldwide cause of pandemic disease and mortality. With the emergence of different influenza strains, it is vital to develop a method using antibodies that can differentiate between viral types and subtypes. Ideally, such a system would also be user friendly. In this study, a polyclonal antibody generated against A/Udorn/307/1972 (H3N2) was used as a probe to distinguish between influenza H3N2 viruses based on the interaction between the antibody and hemagglutinin, demonstrating its applicability for viral discrimination. Clear discrimination was demonstrated using an evanescent-field-coupled waveguide-mode sensor, which has appealing characteristics over other methods in the viewpoint of improving the sensitivity, measurement time, portability and usability. Further supporting evidence was obtained using enzyme-linked immunosorbent assays, hemagglutination-inhibition assays, and infectivity neutralization assays. The results obtained indicate that the polyclonal antibody used here is a potential probe for distinguishing influenza viruses and, with the aid of a handheld sensor it could be used for influenza surveillance.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Noda, T" uniqKey="Noda T">T Noda</name></author><author><name sortKey="Sagara, H" uniqKey="Sagara H">H Sagara</name></author><author><name sortKey="Yen, A" uniqKey="Yen A">A Yen</name></author><author><name sortKey="Takada, A" uniqKey="Takada A">A Takada</name></author><author><name sortKey="Kida, H" uniqKey="Kida H">H Kida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Rivailler, P" uniqKey="Rivailler P">P Rivailler</name></author><author><name sortKey="Conrardy, C" uniqKey="Conrardy C">C Conrardy</name></author><author><name sortKey="Castillo, Da" uniqKey="Castillo D">DA Castillo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, X" uniqKey="Sun X">X Sun</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author><author><name sortKey="He, J" uniqKey="He J">J He</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Biere, B" uniqKey="Biere B">B Biere</name></author><author><name sortKey="Bauer, B" uniqKey="Bauer B">B Bauer</name></author><author><name sortKey="Schweiger, B" uniqKey="Schweiger B">B Schweiger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Sugimoto, K" uniqKey="Sugimoto K">K Sugimoto</name></author><author><name sortKey="Ohki, Y" uniqKey="Ohki Y">Y Ohki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watcharatanyatip, K" uniqKey="Watcharatanyatip K">K Watcharatanyatip</name></author><author><name sortKey="Boonmoh, S" uniqKey="Boonmoh S">S Boonmoh</name></author><author><name sortKey="Chaichoun, K" uniqKey="Chaichoun K">K Chaichoun</name></author><author><name sortKey="Songserm, T" uniqKey="Songserm T">T Songserm</name></author><author><name sortKey="Woratanti, M" uniqKey="Woratanti M">M Woratanti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bahgat, Mm" uniqKey="Bahgat M">MM Bahgat</name></author><author><name sortKey="Kutkat, Ma" uniqKey="Kutkat M">MA Kutkat</name></author><author><name sortKey="Nasraa, Mh" uniqKey="Nasraa M">MH Nasraa</name></author><author><name sortKey="Mostafa, A" uniqKey="Mostafa A">A Mostafa</name></author><author><name sortKey="Webby, R" uniqKey="Webby R">R Webby</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, R" uniqKey="Wang R">R Wang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Lassiter, K" uniqKey="Lassiter K">K Lassiter</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Hargis, B" uniqKey="Hargis B">B Hargis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Sheng, Z" uniqKey="Sheng Z">Z Sheng</name></author><author><name sortKey="Zhang, A" uniqKey="Zhang A">A Zhang</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, M" uniqKey="Watanabe M">M Watanabe</name></author><author><name sortKey="Nakagawa, N" uniqKey="Nakagawa N">N Nakagawa</name></author><author><name sortKey="Ito, M" uniqKey="Ito M">M Ito</name></author><author><name sortKey="Ihara, T" uniqKey="Ihara T">T Ihara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holford, Trj" uniqKey="Holford T">TRJ Holford</name></author><author><name sortKey="Davis, F" uniqKey="Davis F">F Davis</name></author><author><name sortKey="Higson, Spj" uniqKey="Higson S">SPJ Higson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Byrne, B" uniqKey="Byrne B">B Byrne</name></author><author><name sortKey="Stack, E" uniqKey="Stack E">E Stack</name></author><author><name sortKey="Gilmartin, N" uniqKey="Gilmartin N">N Gilmartin</name></author><author><name sortKey="O Ennedy, R" uniqKey="O Ennedy R">R O’Kennedy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kretschmann, E" uniqKey="Kretschmann E">E Kretschmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lukosz, W" uniqKey="Lukosz W">W Lukosz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bolduc, Or" uniqKey="Bolduc O">OR Bolduc</name></author><author><name sortKey="Live, Ls" uniqKey="Live L">LS Live</name></author><author><name sortKey="Masson, J" uniqKey="Masson J">J Masson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Rockstuhl, C" uniqKey="Rockstuhl C">C Rockstuhl</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Fukuda, N" uniqKey="Fukuda N">N Fukuda</name></author><author><name sortKey="Tominaga, J" uniqKey="Tominaga J">J Tominaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Rockstuhl, C" uniqKey="Rockstuhl C">C Rockstuhl</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Tominaga, J" uniqKey="Tominaga J">J Tominaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Nomura, K" uniqKey="Nomura K">K Nomura</name></author><author><name sortKey="Sato, K" uniqKey="Sato K">K Sato</name></author><author><name sortKey="Kato, T" uniqKey="Kato T">T Kato</name></author><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Mizutani, W" uniqKey="Mizutani W">W Mizutani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Fujimaki, M" uniqKey="Fujimaki M">M Fujimaki</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Shima, T" uniqKey="Shima T">T Shima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jenison, Rd" uniqKey="Jenison R">RD Jenison</name></author><author><name sortKey="Gill, Sc" uniqKey="Gill S">SC Gill</name></author><author><name sortKey="Pardi, A" uniqKey="Pardi A">A Pardi</name></author><author><name sortKey="Polisky, B" uniqKey="Polisky B">B Polisky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Geiger, A" uniqKey="Geiger A">A Geiger</name></author><author><name sortKey="Burgstaller, P" uniqKey="Burgstaller P">P Burgstaller</name></author><author><name sortKey="Eltz, Hv" uniqKey="Eltz H">HV Eltz</name></author><author><name sortKey="Roeder, A" uniqKey="Roeder A">A Roeder</name></author><author><name sortKey="Famulok, M" uniqKey="Famulok M">M Famulok</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Misono, T" uniqKey="Misono T">T Misono</name></author><author><name sortKey="Mizuno, T" uniqKey="Mizuno T">T Mizuno</name></author><author><name sortKey="Kawasaki, K" uniqKey="Kawasaki K">K Kawasaki</name></author><author><name sortKey="Kumar, Pkr" uniqKey="Kumar P">PKR Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suenaga, E" uniqKey="Suenaga E">E Suenaga</name></author><author><name sortKey="Mizuno, H" uniqKey="Mizuno H">H Mizuno</name></author><author><name sortKey="Kumar, Pkr" uniqKey="Kumar P">PKR Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gopinath, Scb" uniqKey="Gopinath S">SCB Gopinath</name></author><author><name sortKey="Awazu, K" uniqKey="Awazu K">K Awazu</name></author><author><name sortKey="Kumar, Pkr" uniqKey="Kumar P">PKR Kumar</name></author><author><name sortKey="Tominaga, J" uniqKey="Tominaga J">J Tominaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, M" uniqKey="Imai M">M Imai</name></author><author><name sortKey="Mizuno, T" uniqKey="Mizuno T">T Mizuno</name></author><author><name sortKey="Kawasaki, K" uniqKey="Kawasaki K">K Kawasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De La Rica, R" uniqKey="De La Rica R">R de la Rica</name></author><author><name sortKey="Stevens, Mm" uniqKey="Stevens M">MM Stevens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmeisser, F" uniqKey="Schmeisser F">F Schmeisser</name></author><author><name sortKey="Friedman, R" uniqKey="Friedman R">R Friedman</name></author><author><name sortKey="Besho, J" uniqKey="Besho J">J Besho</name></author><author><name sortKey="Lugovtsev, V" uniqKey="Lugovtsev V">V Lugovtsev</name></author><author><name sortKey="Soto, J" uniqKey="Soto J">J Soto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Appleyard, G" uniqKey="Appleyard G">G Appleyard</name></author><author><name sortKey="Maber, Hb" uniqKey="Maber H">HB Maber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuroda, K" uniqKey="Kuroda K">K Kuroda</name></author><author><name sortKey="Hauser, C" uniqKey="Hauser C">C Hauser</name></author><author><name sortKey="Rott, R" uniqKey="Rott R">R Rott</name></author><author><name sortKey="Klenk, H" uniqKey="Klenk H">H Klenk</name></author><author><name sortKey="Doerfler, W" uniqKey="Doerfler W">W Doerfler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Mukaigawa, J" uniqKey="Mukaigawa J">J Mukaigawa</name></author><author><name sortKey="Oguro, M" uniqKey="Oguro M">M Oguro</name></author><author><name sortKey="Ono, Y" uniqKey="Ono Y">Y Ono</name></author><author><name sortKey="Nakajima, K" uniqKey="Nakajima K">K Nakajima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nishikawa, T" uniqKey="Nishikawa T">T Nishikawa</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T Tanaka</name></author><author><name sortKey="Kuroda, K" uniqKey="Kuroda K">K Kuroda</name></author><author><name sortKey="Takayama, T" uniqKey="Takayama T">T Takayama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, C" uniqKey="Sun C">C Sun</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Chen, K" uniqKey="Chen K">K Chen</name></author><author><name sortKey="He, W" uniqKey="He W">W He</name></author><author><name sortKey="Su, G" uniqKey="Su G">G Su</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24339924</article-id><article-id pub-id-type="pmc">3858306</article-id><article-id pub-id-type="publisher-id">PONE-D-13-22412</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0081396</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor</article-title><alt-title alt-title-type="running-head">Discrimination of Influenza Viruses</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Gopinath</surname><given-names>Subash C. B.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Awazu</surname><given-names>Koichi</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fujimaki</surname><given-names>Makoto</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Shimizu</surname><given-names>Kazufumi</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Open Research Center for Genome and Infectious Disease Control, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Makishima</surname><given-names>Makoto</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Nihon University School of Medicine, Japan</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>gopi-subashchandrabose@aist.go.jp</email> (SCBG); <email>k.awazu@aist.go.jp</email> (KA)</corresp><fn fn-type="COI-statement"><p><bold>Competing Interests: </bold>The authors would also like to thank the Advanced Functional Materials Research Center of Shin-Etsu Chemical Co. Ltd. for supplying the monolithic sensing plate. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: SCBG KA MF KS. Performed the experiments: SCBG KA KS. Analyzed the data: SCBG KS. Contributed reagents/materials/analysis tools: KA MF KS. Wrote the paper: SCBG KA MF KS.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>10</day><month>12</month><year>2013</year></pub-date><volume>8</volume><issue>12</issue><elocation-id>e81396</elocation-id><history><date date-type="received"><day>30</day><month>5</month><year>2013</year></date><date date-type="accepted"><day>11</day><month>10</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 Gopinath et al</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Gopinath et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among infectious diseases, influenza viral infections are a worldwide cause of pandemic disease and mortality. With the emergence of different influenza strains, it is vital to develop a method using antibodies that can differentiate between viral types and subtypes. Ideally, such a system would also be user friendly. In this study, a polyclonal antibody generated against A/Udorn/307/1972 (H3N2) was used as a probe to distinguish between influenza H3N2 viruses based on the interaction between the antibody and hemagglutinin, demonstrating its applicability for viral discrimination. Clear discrimination was demonstrated using an evanescent-field-coupled waveguide-mode sensor, which has appealing characteristics over other methods in the viewpoint of improving the sensitivity, measurement time, portability and usability. Further supporting evidence was obtained using enzyme-linked immunosorbent assays, hemagglutination-inhibition assays, and infectivity neutralization assays. The results obtained indicate that the polyclonal antibody used here is a potential probe for distinguishing influenza viruses and, with the aid of a handheld sensor it could be used for influenza surveillance.</p></abstract><funding-group><funding-statement>This study was supported by the Industrial Technology Research Grant Program 2009 from the New Energy and Industrial Technology Development Organization (NEDO) of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="9"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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