Full Factorial Analysis of Mammalian and Avian Influenza Polymerase Subunits Suggests a Role of an Efficient Polymerase for Virus Adaptation
Identifieur interne : 000A89 ( Pmc/Curation ); précédent : 000A88; suivant : 000A90Full Factorial Analysis of Mammalian and Avian Influenza Polymerase Subunits Suggests a Role of an Efficient Polymerase for Virus Adaptation
Auteurs : Olive T. W. Li [République populaire de Chine] ; Michael C. W. Chan [République populaire de Chine] ; Cynthia S. W. Leung [République populaire de Chine] ; Renee W. Y. Chan [République populaire de Chine] ; Yi Guan [République populaire de Chine] ; John M. Nicholls [République populaire de Chine] ; Leo L. M. Poon [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2009.
Abstract
Amongst all the internal gene segments (PB2. PB1, PA, NP, M and NS), the avian PB1 segment is the only one which was reassorted into the human H2N2 and H3N2 pandemic strains. This suggests that the reassortment of polymerase subunit genes between mammalian and avian influenza viruses might play roles for interspecies transmission. To test this hypothesis, we tested the compatibility between PB2, PB1, PA and NP derived from a H5N1 virus and a mammalian H1N1 virus. All 16 possible combinations of avian-mammalian chimeric viral ribonucleoproteins (vRNPs) were characterized. We showed that recombinant vRNPs with a mammalian PB2 and an avian PB1 had the strongest polymerase activities in human cells at all studied temperature. In addition, viruses with this specific PB2-PB1 combination could grow efficiently in cell cultures, especially at a high incubation temperature. These viruses were potent inducers of proinflammatory cytokines and chemokines in primary human macrophages and pneumocytes. Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure. These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern.
Url:
DOI: 10.1371/journal.pone.0005658
PubMed: 19462010
PubMed Central: 2680953
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W." last="Li">Olive T. W. Li</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Michael C W" sort="Chan, Michael C W" uniqKey="Chan M" first="Michael C. W." last="Chan">Michael C. W. Chan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Leung, Cynthia S W" sort="Leung, Cynthia S W" uniqKey="Leung C" first="Cynthia S. W." last="Leung">Cynthia S. W. Leung</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Pathology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Amongst all the internal gene segments (PB2. PB1, PA, NP, M and NS), the avian PB1 segment is the only one which was reassorted into the human H2N2 and H3N2 pandemic strains. This suggests that the reassortment of polymerase subunit genes between mammalian and avian influenza viruses might play roles for interspecies transmission. To test this hypothesis, we tested the compatibility between PB2, PB1, PA and NP derived from a H5N1 virus and a mammalian H1N1 virus. All 16 possible combinations of avian-mammalian chimeric viral ribonucleoproteins (vRNPs) were characterized. We showed that recombinant vRNPs with a mammalian PB2 and an avian PB1 had the strongest polymerase activities in human cells at all studied temperature. In addition, viruses with this specific PB2-PB1 combination could grow efficiently in cell cultures, especially at a high incubation temperature. These viruses were potent inducers of proinflammatory cytokines and chemokines in primary human macrophages and pneumocytes. Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure. These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author><author><name sortKey="Munster, V" uniqKey="Munster V">V Munster</name></author><author><name sortKey="Wallensten, A" uniqKey="Wallensten A">A Wallensten</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Herfst, S" uniqKey="Herfst S">S Herfst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author><author><name 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Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Castrucci, Mr" uniqKey="Castrucci M">MR Castrucci</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yano, T" uniqKey="Yano T">T Yano</name></author><author><name sortKey="Nobusawa, E" uniqKey="Nobusawa E">E Nobusawa</name></author><author><name sortKey="Nagy, A" uniqKey="Nagy A">A Nagy</name></author><author><name sortKey="Nakajima, S" uniqKey="Nakajima S">S Nakajima</name></author><author><name sortKey="Nakajima, K" uniqKey="Nakajima K">K Nakajima</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19462010</article-id><article-id pub-id-type="pmc">2680953</article-id><article-id pub-id-type="publisher-id">09-PONE-RA-08545R1</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0005658</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline"><subject>Virology</subject><subject>Virology/Effects of Virus Infection on Host Gene Expression</subject><subject>Virology/Viral Replication and Gene Regulation</subject><subject>Virology/Virulence Factors and Mechanisms</subject></subj-group></article-categories><title-group><article-title>Full Factorial Analysis of Mammalian and Avian Influenza Polymerase Subunits Suggests a Role of an Efficient Polymerase for Virus Adaptation</article-title><alt-title alt-title-type="running-head">Chimeric Influenza vRNPs</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Li</surname><given-names>Olive T. W.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Michael C. W.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Leung</surname><given-names>Cynthia S. W.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Renee W. Y.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Guan</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nicholls</surname><given-names>John M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Poon</surname><given-names>Leo L. M.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Pathology, The University of Hong Kong, Hong Kong SAR, China</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Liu</surname><given-names>Ding Xiang</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1">Institute of Molecular and Cell Biology, Singapore</aff><author-notes><corresp id="cor1">* E-mail: <email>llmpoon@hkucc.hku.hk</email></corresp><fn fn-type="con"><p>Conceived and designed the experiments: OTWL MCWC CSWL YG LLMP. Performed the experiments: OTWL MCWC CSWL RWYC JMN. Analyzed the data: OTWL MCWC CSWL RWYC YG JMN LLMP. Contributed reagents/materials/analysis tools: OTWL MCWC JMN LLMP. Wrote the paper: OTWL LLMP.</p></fn></author-notes><pub-date pub-type="collection"><year>2009</year></pub-date><pub-date pub-type="epub"><day>21</day><month>5</month><year>2009</year></pub-date><volume>4</volume><issue>5</issue><elocation-id>e5658</elocation-id><history><date date-type="received"><day>5</day><month>2</month><year>2009</year></date><date date-type="accepted"><day>16</day><month>4</month><year>2009</year></date></history><permissions><copyright-statement>Li et al.</copyright-statement><copyright-year>2009</copyright-year><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>Amongst all the internal gene segments (PB2. PB1, PA, NP, M and NS), the avian PB1 segment is the only one which was reassorted into the human H2N2 and H3N2 pandemic strains. This suggests that the reassortment of polymerase subunit genes between mammalian and avian influenza viruses might play roles for interspecies transmission. To test this hypothesis, we tested the compatibility between PB2, PB1, PA and NP derived from a H5N1 virus and a mammalian H1N1 virus. All 16 possible combinations of avian-mammalian chimeric viral ribonucleoproteins (vRNPs) were characterized. We showed that recombinant vRNPs with a mammalian PB2 and an avian PB1 had the strongest polymerase activities in human cells at all studied temperature. In addition, viruses with this specific PB2-PB1 combination could grow efficiently in cell cultures, especially at a high incubation temperature. These viruses were potent inducers of proinflammatory cytokines and chemokines in primary human macrophages and pneumocytes. Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure. These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern.</p></abstract><counts><page-count count="13"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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