Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets
Identifieur interne : 000A59 ( Pmc/Curation ); précédent : 000A58; suivant : 000A60Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets
Auteurs : S. K. Rosendahl Huber [Pays-Bas] ; M. G. M. Camps [Pays-Bas] ; R. H. J. Jacobi [Pays-Bas] ; J. Mouthaan [Pays-Bas] ; H. Van Dijken [Pays-Bas] ; J. Van Beek [Pays-Bas] ; F. Ossendorp [Pays-Bas] ; J. De Jonge [Pays-Bas]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Currently licensed influenza vaccines mainly induce antibodies against highly variable epitopes. Due to antigenic drift, protection is subtype or strain-specific and regular vaccine updates are required. In case of antigenic shifts, which have caused several pandemics in the past, completely new vaccines need to be developed. We set out to develop a vaccine that provides protection against a broad range of influenza viruses. Therefore, highly conserved parts of the influenza A virus (IAV) were selected of which we constructed antibody and T cell inducing peptide-based vaccines. The B epitope vaccine consists of the highly conserved HA2 fusion peptide and M2e peptide coupled to a CD4 helper epitope. The T epitope vaccine comprises 25 overlapping synthetic long peptides of 26-34 amino acids, thereby avoiding restriction for a certain MHC haplotype. These peptides are derived from nucleoprotein (NP), polymerase basic protein 1 (PB1) and matrix protein 1 (M1). C57BL/6 mice, BALB/c mice, and ferrets were vaccinated with the B epitopes, 25 SLP or a combination of both. Vaccine-specific antibodies were detected in sera of mice and ferrets and vaccine-specific cellular responses were measured in mice. Following challenge, both mice and ferrets showed a reduction of virus titers in the lungs in response to vaccination. Summarizing, a peptide-based vaccine directed against conserved parts of influenza virus containing B and T cell epitopes shows promising results for further development. Such a vaccine may reduce disease burden and virus transmission during pandemic outbreaks.
Url:
DOI: 10.1371/journal.pone.0127969
PubMed: 26046664
PubMed Central: 4457525
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A59
Links to Exploration step
PMC:4457525Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets</title><author><name sortKey="Rosendahl Huber, S K" sort="Rosendahl Huber, S K" uniqKey="Rosendahl Huber S" first="S. K." last="Rosendahl Huber">S. K. Rosendahl Huber</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Camps, M G M" sort="Camps, M G M" uniqKey="Camps M" first="M. G. M." last="Camps">M. G. M. Camps</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden</wicri:regionArea></affiliation></author><author><name sortKey="Jacobi, R H J" sort="Jacobi, R H J" uniqKey="Jacobi R" first="R. H. J." last="Jacobi">R. H. J. Jacobi</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Mouthaan, J" sort="Mouthaan, J" uniqKey="Mouthaan J" first="J." last="Mouthaan">J. Mouthaan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Dijken, H" sort="Van Dijken, H" uniqKey="Van Dijken H" first="H." last="Van Dijken">H. Van Dijken</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Beek, J" sort="Van Beek, J" uniqKey="Van Beek J" first="J." last="Van Beek">J. Van Beek</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Ossendorp, F" sort="Ossendorp, F" uniqKey="Ossendorp F" first="F." last="Ossendorp">F. Ossendorp</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden</wicri:regionArea></affiliation></author><author><name sortKey="De Jonge, J" sort="De Jonge, J" uniqKey="De Jonge J" first="J." last="De Jonge">J. De Jonge</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26046664</idno><idno type="pmc">4457525</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457525</idno><idno type="RBID">PMC:4457525</idno><idno type="doi">10.1371/journal.pone.0127969</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000A59</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A59</idno><idno type="wicri:Area/Pmc/Curation">000A59</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A59</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets</title><author><name sortKey="Rosendahl Huber, S K" sort="Rosendahl Huber, S K" uniqKey="Rosendahl Huber S" first="S. K." last="Rosendahl Huber">S. K. Rosendahl Huber</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Camps, M G M" sort="Camps, M G M" uniqKey="Camps M" first="M. G. M." last="Camps">M. G. M. Camps</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden</wicri:regionArea></affiliation></author><author><name sortKey="Jacobi, R H J" sort="Jacobi, R H J" uniqKey="Jacobi R" first="R. H. J." last="Jacobi">R. H. J. Jacobi</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Mouthaan, J" sort="Mouthaan, J" uniqKey="Mouthaan J" first="J." last="Mouthaan">J. Mouthaan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Dijken, H" sort="Van Dijken, H" uniqKey="Van Dijken H" first="H." last="Van Dijken">H. Van Dijken</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Beek, J" sort="Van Beek, J" uniqKey="Van Beek J" first="J." last="Van Beek">J. Van Beek</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Ossendorp, F" sort="Ossendorp, F" uniqKey="Ossendorp F" first="F." last="Ossendorp">F. Ossendorp</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden</wicri:regionArea></affiliation></author><author><name sortKey="De Jonge, J" sort="De Jonge, J" uniqKey="De Jonge J" first="J." last="De Jonge">J. De Jonge</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Currently licensed influenza vaccines mainly induce antibodies against highly variable epitopes. Due to antigenic drift, protection is subtype or strain-specific and regular vaccine updates are required. In case of antigenic shifts, which have caused several pandemics in the past, completely new vaccines need to be developed. We set out to develop a vaccine that provides protection against a broad range of influenza viruses. Therefore, highly conserved parts of the influenza A virus (IAV) were selected of which we constructed antibody and T cell inducing peptide-based vaccines. The B epitope vaccine consists of the highly conserved HA2 fusion peptide and M2e peptide coupled to a CD4 helper epitope. The T epitope vaccine comprises 25 overlapping synthetic long peptides of 26-34 amino acids, thereby avoiding restriction for a certain MHC haplotype. These peptides are derived from nucleoprotein (NP), polymerase basic protein 1 (PB1) and matrix protein 1 (M1). C57BL/6 mice, BALB/c mice, and ferrets were vaccinated with the B epitopes, 25 SLP or a combination of both. Vaccine-specific antibodies were detected in sera of mice and ferrets and vaccine-specific cellular responses were measured in mice. Following challenge, both mice and ferrets showed a reduction of virus titers in the lungs in response to vaccination. Summarizing, a peptide-based vaccine directed against conserved parts of influenza virus containing B and T cell epitopes shows promising results for further development. Such a vaccine may reduce disease burden and virus transmission during pandemic outbreaks.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Plotkin, Sa" uniqKey="Plotkin S">SA Plotkin</name></author><author><name sortKey="Orenstein, Wa" uniqKey="Orenstein W">WA Orenstein</name></author><author><name sortKey="Offit, Pa" uniqKey="Offit P">PA Offit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quinones Parra, S" uniqKey="Quinones Parra S">S Quinones-Parra</name></author><author><name sortKey="Loh, L" uniqKey="Loh L">L Loh</name></author><author><name sortKey="Brown, Le" uniqKey="Brown L">LE Brown</name></author><author><name sortKey="Kedzierska, K" uniqKey="Kedzierska K">K Kedzierska</name></author><author><name sortKey="Valkenburg, Sa" uniqKey="Valkenburg S">SA Valkenburg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chun, S" uniqKey="Chun S">S Chun</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author><author><name sortKey="Van Domselaar, G" uniqKey="Van Domselaar G">G Van Domselaar</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Farnsworth, A" uniqKey="Farnsworth A">A Farnsworth</name></author><author><name sortKey="Cui, X" uniqKey="Cui X">X Cui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cross, Kj" uniqKey="Cross K">KJ Cross</name></author><author><name sortKey="Langley, Wa" uniqKey="Langley W">WA Langley</name></author><author><name sortKey="Russell, Rj" uniqKey="Russell R">RJ Russell</name></author><author><name sortKey="Skehel, Jj" uniqKey="Skehel J">JJ Skehel</name></author><author><name sortKey="Steinhauer, Da" uniqKey="Steinhauer D">DA Steinhauer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fiers, W" uniqKey="Fiers W">W Fiers</name></author><author><name sortKey="De Filette, M" uniqKey="De Filette M">M De Filette</name></author><author><name sortKey="Birkett, A" uniqKey="Birkett A">A Birkett</name></author><author><name sortKey="Neirynck, S" uniqKey="Neirynck S">S Neirynck</name></author><author><name sortKey="Min, Jou W" uniqKey="Min J">Jou W Min</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Joseph, T" uniqKey="Joseph T">T Joseph</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jegerlehner, A" uniqKey="Jegerlehner A">A Jegerlehner</name></author><author><name sortKey="Schmitz, N" uniqKey="Schmitz N">N Schmitz</name></author><author><name sortKey="Storni, T" uniqKey="Storni T">T Storni</name></author><author><name sortKey="Bachmann, Mf" uniqKey="Bachmann M">MF Bachmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sukeno, N" uniqKey="Sukeno N">N Sukeno</name></author><author><name sortKey="Otsuki, Y" uniqKey="Otsuki Y">Y Otsuki</name></author><author><name sortKey="Konno, J" uniqKey="Konno J">J Konno</name></author><author><name sortKey="Yamane, N" uniqKey="Yamane N">N Yamane</name></author><author><name sortKey="Odagiri, T" uniqKey="Odagiri T">T Odagiri</name></author><author><name sortKey="Arikawa, J" uniqKey="Arikawa J">J Arikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stanekova, Z" uniqKey="Stanekova Z">Z Stanekova</name></author><author><name sortKey="Vareckova, E" uniqKey="Vareckova E">E Vareckova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grant, E" uniqKey="Grant E">E Grant</name></author><author><name sortKey="Wu, C" uniqKey="Wu C">C Wu</name></author><author><name sortKey="Chan, Kf" uniqKey="Chan K">KF Chan</name></author><author><name sortKey="Eckle, S" uniqKey="Eckle S">S Eckle</name></author><author><name sortKey="Bharadwaj, M" uniqKey="Bharadwaj M">M Bharadwaj</name></author><author><name sortKey="Zou, Qm" uniqKey="Zou Q">QM Zou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Assarsson, E" uniqKey="Assarsson E">E Assarsson</name></author><author><name sortKey="Bui, Hh" uniqKey="Bui H">HH Bui</name></author><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Glenn, J" uniqKey="Glenn J">J Glenn</name></author><author><name sortKey="Oseroff, C" uniqKey="Oseroff C">C Oseroff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sridhar, S" uniqKey="Sridhar S">S Sridhar</name></author><author><name sortKey="Begom, S" uniqKey="Begom S">S Begom</name></author><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name></author><author><name sortKey="Hoschler, K" uniqKey="Hoschler K">K Hoschler</name></author><author><name sortKey="Adamson, W" uniqKey="Adamson W">W Adamson</name></author><author><name sortKey="Carman, W" uniqKey="Carman W">W Carman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilkinson, Tm" uniqKey="Wilkinson T">TM Wilkinson</name></author><author><name sortKey="Li, Ck" uniqKey="Li C">CK Li</name></author><author><name sortKey="Chui, Cs" uniqKey="Chui C">CS Chui</name></author><author><name sortKey="Huang, Ak" uniqKey="Huang A">AK Huang</name></author><author><name sortKey="Perkins, M" uniqKey="Perkins M">M Perkins</name></author><author><name sortKey="Liebner, Jc" uniqKey="Liebner J">JC Liebner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kenter, Gg" uniqKey="Kenter G">GG Kenter</name></author><author><name sortKey="Welters, Mj" uniqKey="Welters M">MJ Welters</name></author><author><name sortKey="Valentijn, Ar" uniqKey="Valentijn A">AR Valentijn</name></author><author><name sortKey="Lowik, Mj" uniqKey="Lowik M">MJ Lowik</name></author><author><name sortKey="Berends Van Der Meer, Dm" uniqKey="Berends Van Der Meer D">DM Berends-van der Meer</name></author><author><name sortKey="Vloon, Ap" uniqKey="Vloon A">AP Vloon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bijker, Ms" uniqKey="Bijker M">MS Bijker</name></author><author><name sortKey="Van Den Eeden, Sj" uniqKey="Van Den Eeden S">SJ van den Eeden</name></author><author><name sortKey="Franken, Kl" uniqKey="Franken K">KL Franken</name></author><author><name sortKey="Melief, Cj" uniqKey="Melief C">CJ Melief</name></author><author><name sortKey="Van Der Burg, Sh" uniqKey="Van Der Burg S">SH van der Burg</name></author><author><name sortKey="Offringa, R" uniqKey="Offringa R">R Offringa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bijker, Ms" uniqKey="Bijker M">MS Bijker</name></author><author><name sortKey="Van Den Eeden, Sj" uniqKey="Van Den Eeden S">SJ van den Eeden</name></author><author><name sortKey="Franken, Kl" uniqKey="Franken K">KL Franken</name></author><author><name sortKey="Melief, Cj" uniqKey="Melief C">CJ Melief</name></author><author><name sortKey="Offringa, R" uniqKey="Offringa R">R Offringa</name></author><author><name sortKey="Van Der Burg, Sh" uniqKey="Van Der Burg S">SH van der Burg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, Ma" uniqKey="Williams M">MA Williams</name></author><author><name sortKey="Bevan, Mj" uniqKey="Bevan M">MJ Bevan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakanishi, Y" uniqKey="Nakanishi Y">Y Nakanishi</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name></author><author><name sortKey="Gerard, C" uniqKey="Gerard C">C Gerard</name></author><author><name sortKey="Iwasaki, A" uniqKey="Iwasaki A">A Iwasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schuurhuis, Dh" uniqKey="Schuurhuis D">DH Schuurhuis</name></author><author><name sortKey="Laban, S" uniqKey="Laban S">S Laban</name></author><author><name sortKey="Toes, Re" uniqKey="Toes R">RE Toes</name></author><author><name sortKey="Ricciardi Castagnoli, P" uniqKey="Ricciardi Castagnoli P">P Ricciardi-Castagnoli</name></author><author><name sortKey="Kleijmeer, Mj" uniqKey="Kleijmeer M">MJ Kleijmeer</name></author><author><name sortKey="Van Der Voort, Ei" uniqKey="Van Der Voort E">EI van der Voort</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ossendorp, F" uniqKey="Ossendorp F">F Ossendorp</name></author><author><name sortKey="Mengede, E" uniqKey="Mengede E">E Mengede</name></author><author><name sortKey="Camps, M" uniqKey="Camps M">M Camps</name></author><author><name sortKey="Filius, R" uniqKey="Filius R">R Filius</name></author><author><name sortKey="Melief, Cj" uniqKey="Melief C">CJ Melief</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alexander, J" uniqKey="Alexander J">J Alexander</name></author><author><name sortKey="Bilsel, P" uniqKey="Bilsel P">P Bilsel</name></author><author><name sortKey="Del Guercio, Mf" uniqKey="Del Guercio M">MF del Guercio</name></author><author><name sortKey="Marinkovic Petrovic, A" uniqKey="Marinkovic Petrovic A">A Marinkovic-Petrovic</name></author><author><name sortKey="Southwood, S" uniqKey="Southwood S">S Southwood</name></author><author><name sortKey="Stewart, S" uniqKey="Stewart S">S Stewart</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lamberth, K" uniqKey="Lamberth K">K Lamberth</name></author><author><name sortKey="Harndahl, M" uniqKey="Harndahl M">M Harndahl</name></author><author><name sortKey="Roder, G" uniqKey="Roder G">G Roder</name></author><author><name sortKey="Stryhn, A" uniqKey="Stryhn A">A Stryhn</name></author><author><name sortKey="Larsen, Mv" uniqKey="Larsen M">MV Larsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Ly" uniqKey="Lee L">LY Lee</name></author><author><name sortKey="Ha Do, La" uniqKey="Ha Do L">LA Ha do</name></author><author><name sortKey="Simmons, C" uniqKey="Simmons C">C Simmons</name></author><author><name sortKey="De Jong, Md" uniqKey="De Jong M">MD de Jong</name></author><author><name sortKey="Chau, Nv" uniqKey="Chau N">NV Chau</name></author><author><name sortKey="Schumacher, R" uniqKey="Schumacher R">R Schumacher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hiemstra, Hs" uniqKey="Hiemstra H">HS Hiemstra</name></author><author><name sortKey="Duinkerken, G" uniqKey="Duinkerken G">G Duinkerken</name></author><author><name sortKey="Benckhuijsen, We" uniqKey="Benckhuijsen W">WE Benckhuijsen</name></author><author><name sortKey="Amons, R" uniqKey="Amons R">R Amons</name></author><author><name sortKey="De Vries, Rr" uniqKey="De Vries R">RR de Vries</name></author><author><name sortKey="Roep, Bo" uniqKey="Roep B">BO Roep</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alexander, J" uniqKey="Alexander J">J Alexander</name></author><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author><author><name sortKey="Southwood, S" uniqKey="Southwood S">S Southwood</name></author><author><name sortKey="Ruppert, J" uniqKey="Ruppert J">J Ruppert</name></author><author><name sortKey="Oseroff, C" uniqKey="Oseroff C">C Oseroff</name></author><author><name sortKey="Maewal, A" uniqKey="Maewal A">A Maewal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Den Brand, Jm" uniqKey="Van Den Brand J">JM van den Brand</name></author><author><name sortKey="Stittelaar, Kj" uniqKey="Stittelaar K">KJ Stittelaar</name></author><author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G van Amerongen</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author><author><name sortKey="Simon, J" uniqKey="Simon J">J Simon</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdel Ghafar, An" uniqKey="Abdel Ghafar A">AN Abdel-Ghafar</name></author><author><name sortKey="Chotpitayasunondh, T" uniqKey="Chotpitayasunondh T">T Chotpitayasunondh</name></author><author><name sortKey="Gao, Z" uniqKey="Gao Z">Z Gao</name></author><author><name sortKey="Hayden, Fg" uniqKey="Hayden F">FG Hayden</name></author><author><name sortKey="Nguyen, Dh" uniqKey="Nguyen D">DH Nguyen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Ljm H" uniqKey="Reed L">LJM, H. Reed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Finkelman, Fd" uniqKey="Finkelman F">FD Finkelman</name></author><author><name sortKey="Katona, Im" uniqKey="Katona I">IM Katona</name></author><author><name sortKey="Mosmann, Tr" uniqKey="Mosmann T">TR Mosmann</name></author><author><name sortKey="Coffman, Rl" uniqKey="Coffman R">RL Coffman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snapper, Cm" uniqKey="Snapper C">CM Snapper</name></author><author><name sortKey="Finkelman, Fd" uniqKey="Finkelman F">FD Finkelman</name></author><author><name sortKey="Paul, We" uniqKey="Paul W">WE Paul</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sigal, Lj" uniqKey="Sigal L">LJ Sigal</name></author><author><name sortKey="Goebel, P" uniqKey="Goebel P">P Goebel</name></author><author><name sortKey="Wylie, De" uniqKey="Wylie D">DE Wylie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nielsen, M" uniqKey="Nielsen M">M Nielsen</name></author><author><name sortKey="Lundegaard, C" uniqKey="Lundegaard C">C Lundegaard</name></author><author><name sortKey="Worning, P" uniqKey="Worning P">P Worning</name></author><author><name sortKey="Lauemoller, Sl" uniqKey="Lauemoller S">SL Lauemoller</name></author><author><name sortKey="Lamberth, K" uniqKey="Lamberth K">K Lamberth</name></author><author><name sortKey="Buus, S" uniqKey="Buus S">S Buus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bodewes, R" uniqKey="Bodewes R">R Bodewes</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steel, J" uniqKey="Steel J">J Steel</name></author><author><name sortKey="Lowen, Ac" uniqKey="Lowen A">AC Lowen</name></author><author><name sortKey="Wang, Tt" uniqKey="Wang T">TT Wang</name></author><author><name sortKey="Yondola, M" uniqKey="Yondola M">M Yondola</name></author><author><name sortKey="Gao, Q" uniqKey="Gao Q">Q Gao</name></author><author><name sortKey="Haye, K" uniqKey="Haye K">K Haye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hillaire, Ml" uniqKey="Hillaire M">ML Hillaire</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Atassi, Mz" uniqKey="Atassi M">MZ Atassi</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stanekova, Z" uniqKey="Stanekova Z">Z Stanekova</name></author><author><name sortKey="Kiraly, J" uniqKey="Kiraly J">J Kiraly</name></author><author><name sortKey="Stropkovska, A" uniqKey="Stropkovska A">A Stropkovska</name></author><author><name sortKey="Mikuskova, T" uniqKey="Mikuskova T">T Mikuskova</name></author><author><name sortKey="Mucha, V" uniqKey="Mucha V">V Mucha</name></author><author><name sortKey="Kostolansky, F" uniqKey="Kostolansky F">F Kostolansky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stanekova, Z" uniqKey="Stanekova Z">Z Stanekova</name></author><author><name sortKey="Mucha, V" uniqKey="Mucha V">V Mucha</name></author><author><name sortKey="Sladkova, T" uniqKey="Sladkova T">T Sladkova</name></author><author><name sortKey="Blaskovicova, H" uniqKey="Blaskovicova H">H Blaskovicova</name></author><author><name sortKey="Kostolansky, F" uniqKey="Kostolansky F">F Kostolansky</name></author><author><name sortKey="Vareckova, E" uniqKey="Vareckova E">E Vareckova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stoloff, Ga" uniqKey="Stoloff G">GA Stoloff</name></author><author><name sortKey="Caparros Wanderley, W" uniqKey="Caparros Wanderley W">W Caparros-Wanderley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ichihashi, T" uniqKey="Ichihashi T">T Ichihashi</name></author><author><name sortKey="Yoshida, R" uniqKey="Yoshida R">R Yoshida</name></author><author><name sortKey="Sugimoto, C" uniqKey="Sugimoto C">C Sugimoto</name></author><author><name sortKey="Takada, A" uniqKey="Takada A">A Takada</name></author><author><name sortKey="Kajino, K" uniqKey="Kajino K">K Kajino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsui, M" uniqKey="Matsui M">M Matsui</name></author><author><name sortKey="Kohyama, S" uniqKey="Kohyama S">S Kohyama</name></author><author><name sortKey="Suda, T" uniqKey="Suda T">T Suda</name></author><author><name sortKey="Yokoyama, S" uniqKey="Yokoyama S">S Yokoyama</name></author><author><name sortKey="Mori, M" uniqKey="Mori M">M Mori</name></author><author><name sortKey="Kobayashi, A" uniqKey="Kobayashi A">A Kobayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Ac" uniqKey="Tan A">AC Tan</name></author><author><name sortKey="Deliyannis, G" uniqKey="Deliyannis G">G Deliyannis</name></author><author><name sortKey="Bharadwaj, M" uniqKey="Bharadwaj M">M Bharadwaj</name></author><author><name sortKey="Brown, Le" uniqKey="Brown L">LE Brown</name></author><author><name sortKey="Zeng, W" uniqKey="Zeng W">W Zeng</name></author><author><name sortKey="Jackson, Dc" uniqKey="Jackson D">DC Jackson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Plotnicky, H" uniqKey="Plotnicky H">H Plotnicky</name></author><author><name sortKey="Cyblat Chanal, D" uniqKey="Cyblat Chanal D">D Cyblat-Chanal</name></author><author><name sortKey="Aubry, Jp" uniqKey="Aubry J">JP Aubry</name></author><author><name sortKey="Derouet, F" uniqKey="Derouet F">F Derouet</name></author><author><name sortKey="Klinguer Hamour, C" uniqKey="Klinguer Hamour C">C Klinguer-Hamour</name></author><author><name sortKey="Beck, A" uniqKey="Beck A">A Beck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pica, N" uniqKey="Pica N">N Pica</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pleguezuelos, O" uniqKey="Pleguezuelos O">O Pleguezuelos</name></author><author><name sortKey="Robinson, S" uniqKey="Robinson S">S Robinson</name></author><author><name sortKey="Stoloff, Ga" uniqKey="Stoloff G">GA Stoloff</name></author><author><name sortKey="Caparros Wanderley, W" uniqKey="Caparros Wanderley W">W Caparros-Wanderley</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ebrahimi, Sm" uniqKey="Ebrahimi S">SM Ebrahimi</name></author><author><name sortKey="Tebianian, M" uniqKey="Tebianian M">M Tebianian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jegaskanda, S" uniqKey="Jegaskanda S">S Jegaskanda</name></author><author><name sortKey="Job, Er" uniqKey="Job E">ER Job</name></author><author><name sortKey="Kramski, M" uniqKey="Kramski M">M Kramski</name></author><author><name sortKey="Laurie, K" uniqKey="Laurie K">K Laurie</name></author><author><name sortKey="Isitman, G" uniqKey="Isitman G">G Isitman</name></author><author><name sortKey="De Rose, R" uniqKey="De Rose R">R de Rose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laidlaw, Bj" uniqKey="Laidlaw B">BJ Laidlaw</name></author><author><name sortKey="Decman, V" uniqKey="Decman V">V Decman</name></author><author><name sortKey="Ali, Ma" uniqKey="Ali M">MA Ali</name></author><author><name sortKey="Abt, Mc" uniqKey="Abt M">MC Abt</name></author><author><name sortKey="Wolf, Ai" uniqKey="Wolf A">AI Wolf</name></author><author><name sortKey="Monticelli, La" uniqKey="Monticelli L">LA Monticelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suda, T" uniqKey="Suda T">T Suda</name></author><author><name sortKey="Kawano, M" uniqKey="Kawano M">M Kawano</name></author><author><name sortKey="Nogi, Y" uniqKey="Nogi Y">Y Nogi</name></author><author><name sortKey="Ohno, N" uniqKey="Ohno N">N Ohno</name></author><author><name sortKey="Akatsuka, T" uniqKey="Akatsuka T">T Akatsuka</name></author><author><name sortKey="Matsui, M" uniqKey="Matsui M">M Matsui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolf, Ai" uniqKey="Wolf A">AI Wolf</name></author><author><name sortKey="Mozdzanowska, K" uniqKey="Mozdzanowska K">K Mozdzanowska</name></author><author><name sortKey="Williams, Kl" uniqKey="Williams K">KL Williams</name></author><author><name sortKey="Singer, D" uniqKey="Singer D">D Singer</name></author><author><name sortKey="Richter, M" uniqKey="Richter M">M Richter</name></author><author><name sortKey="Hoffmann, R" uniqKey="Hoffmann R">R Hoffmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Purcell, Aw" uniqKey="Purcell A">AW Purcell</name></author><author><name sortKey="Mccluskey, J" uniqKey="Mccluskey J">J McCluskey</name></author><author><name sortKey="Rossjohn, J" uniqKey="Rossjohn J">J Rossjohn</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26046664</article-id><article-id pub-id-type="pmc">4457525</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0127969</article-id><article-id pub-id-type="publisher-id">PONE-D-15-07531</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets</article-title><alt-title alt-title-type="running-head">Synthetic Long Peptide-Based Influenza Vaccine</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Rosendahl Huber</surname><given-names>S. K.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Camps</surname><given-names>M. G. M.</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jacobi</surname><given-names>R. H. J.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mouthaan</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>van Dijken</surname><given-names>H.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>van Beek</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ossendorp</surname><given-names>F.</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="author-notes" rid="econtrib001"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>de Jonge</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="econtrib001"><sup>‡</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, the Netherlands</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Kang</surname><given-names>Sang-Moo</given-names></name><role>Academic Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Georgia State University, UNITED STATES</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: SRH MC FO JvB JdJ. Performed the experiments: SRH MC RJ JM HvD JdJ. Analyzed the data: SRH JdJ. Contributed reagents/materials/analysis tools: MC FO. Wrote the paper: SRH FO JvB JdJ.</p></fn><fn fn-type="other" id="econtrib001"><p>‡ These authors are shared senior authors on this work.</p></fn><corresp id="cor001">* E-mail: <email>Jorgen.de.Jonge@rivm.nl</email></corresp></author-notes><pub-date pub-type="epub"><day>5</day><month>6</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>10</volume><issue>6</issue><elocation-id>e0127969</elocation-id><history><date date-type="received"><day>19</day><month>2</month><year>2015</year></date><date date-type="accepted"><day>22</day><month>4</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 Rosendahl Huber et al</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>Rosendahl Huber et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0127969.pdf"></self-uri><abstract><p>Currently licensed influenza vaccines mainly induce antibodies against highly variable epitopes. Due to antigenic drift, protection is subtype or strain-specific and regular vaccine updates are required. In case of antigenic shifts, which have caused several pandemics in the past, completely new vaccines need to be developed. We set out to develop a vaccine that provides protection against a broad range of influenza viruses. Therefore, highly conserved parts of the influenza A virus (IAV) were selected of which we constructed antibody and T cell inducing peptide-based vaccines. The B epitope vaccine consists of the highly conserved HA2 fusion peptide and M2e peptide coupled to a CD4 helper epitope. The T epitope vaccine comprises 25 overlapping synthetic long peptides of 26-34 amino acids, thereby avoiding restriction for a certain MHC haplotype. These peptides are derived from nucleoprotein (NP), polymerase basic protein 1 (PB1) and matrix protein 1 (M1). C57BL/6 mice, BALB/c mice, and ferrets were vaccinated with the B epitopes, 25 SLP or a combination of both. Vaccine-specific antibodies were detected in sera of mice and ferrets and vaccine-specific cellular responses were measured in mice. Following challenge, both mice and ferrets showed a reduction of virus titers in the lungs in response to vaccination. Summarizing, a peptide-based vaccine directed against conserved parts of influenza virus containing B and T cell epitopes shows promising results for further development. Such a vaccine may reduce disease burden and virus transmission during pandemic outbreaks.</p></abstract><funding-group><funding-statement>Funding provided to FO, JvB, JdJ by the European Union Seventh Framework Programme under grant number 201169, Identification of Mechanisms Correlating with Susceptibility for Avian Influenza (IMECS), <ext-link ext-link-type="uri" xlink:href="http://ec.europa.eu/research/health/infectious-diseases/emerging-epidemics/projects/175_en.html">http://ec.europa.eu/research/health/infectious-diseases/emerging-epidemics/projects/175_en.html</ext-link>. Funding provided to JdJ, HvD, JM by the Health Program of the European Union under grant number 2009 1106, Fast Vaccination (FASTVAC), <ext-link ext-link-type="uri" xlink:href="http://www.fastvac.eu/work-packages/liaison-for-vaccine-deployment">http://www.fastvac.eu/work-packages/liaison-for-vaccine-deployment</ext-link>. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="6"></fig-count><table-count count="2"></table-count><page-count count="19"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A59 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000A59 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4457525
|texte= Synthetic Long Peptide Influenza Vaccine Containing Conserved T and B Cell Epitopes Reduces Viral Load in Lungs of Mice and Ferrets
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:26046664" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |