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Blood Screening for Influenza

Identifieur interne : 000493 ( Pmc/Curation ); précédent : 000492; suivant : 000494

Blood Screening for Influenza

Auteurs : Michael Kai Hourfar [Allemagne] ; Anna Themann [Allemagne] ; Markus Eickmann [Allemagne] ; Pilaipan Puthavathana [Thaïlande] ; Thomas Laue [Allemagne] ; Erhard Seifried [Allemagne] ; Michael Schmidt [Allemagne]

Source :

RBID : PMC:2878216

Abstract

Influenza viruses, including highly pathogenic avian influenza virus (H5N1), could threaten blood safety. We analyzed 10,272 blood donor samples with a minipool nucleic acid amplication technique. Analytical sensitivity of the method was 804 geq/mL and 444 geq/mL for generic influenza primers and influenza (H5N1) subtype–specific primers. This study demonstrates that such screening for influenza viruses is feasible.


Url:
DOI: 10.3201/eid1307.060861
PubMed: 18214186
PubMed Central: 2878216

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PMC:2878216

Le document en format XML

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<nlm:aff id="aff1">German Red Cross, Frankfurt, Germany</nlm:aff>
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<p>Influenza viruses, including highly pathogenic avian influenza virus (H5N1), could threaten blood safety. We analyzed 10,272 blood donor samples with a minipool nucleic acid amplication technique. Analytical sensitivity of the method was 804 geq/mL and 444 geq/mL for generic influenza primers and influenza (H5N1) subtype–specific primers. This study demonstrates that such screening for influenza viruses is feasible.</p>
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<name>
<surname>Hourfar</surname>
<given-names>Michael Kai</given-names>
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<xref ref-type="aff" rid="aff1">*</xref>
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<name>
<surname>Themann</surname>
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<surname>Puthavathana</surname>
<given-names>Pilaipan</given-names>
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<xref ref-type="aff" rid="aff3"></xref>
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<name>
<surname>Laue</surname>
<given-names>Thomas</given-names>
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<xref ref-type="aff" rid="aff4">§</xref>
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<surname>Seifried</surname>
<given-names>Erhard</given-names>
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German Red Cross, Frankfurt, Germany</aff>
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<label></label>
Phillips University, Marburg, Germany</aff>
<aff id="aff3">
<label></label>
Mahidol University, Bangkok, Thailand</aff>
<aff id="aff4">
<label>§</label>
QIAGEN, Hamburg, Germany</aff>
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<author-notes>
<corresp id="cor1">Address for correspondence: Michael Schmidt, Institute of Transfusion Medicine and Immunohematology, Johann Wolfgang Goethe University, German Red Cross, Sandhofstr. 1, 60528 Frankfurt am Main; Frankfurt, Germany; email:
<email xlink:href="mschmidt@blutspende.de">mschmidt@blutspende.de</email>
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<pub-date pub-type="ppub">
<month>7</month>
<year>2007</year>
</pub-date>
<volume>13</volume>
<issue>7</issue>
<fpage>1081</fpage>
<lpage>1083</lpage>
<abstract>
<p>Influenza viruses, including highly pathogenic avian influenza virus (H5N1), could threaten blood safety. We analyzed 10,272 blood donor samples with a minipool nucleic acid amplication technique. Analytical sensitivity of the method was 804 geq/mL and 444 geq/mL for generic influenza primers and influenza (H5N1) subtype–specific primers. This study demonstrates that such screening for influenza viruses is feasible.</p>
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<title>Keywords: </title>
<kwd>avian influenza</kwd>
<kwd>influenza A (H5N1)</kwd>
<kwd>blood screening</kwd>
<kwd>real-time PCR</kwd>
<kwd>dispatch</kwd>
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