Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses
Identifieur interne : 000454 ( Pmc/Curation ); précédent : 000453; suivant : 000455Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses
Auteurs : Gerard Kian-Meng Goh [États-Unis] ; A Keith Dunker [États-Unis] ; Vladimir N. Uversky [États-Unis, Russie]Source :
- Virology Journal [ 1743-422X ] ; 2009.
Abstract
The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.
Intrinsic disorder predictor PONDR® VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.
The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA2. This region represents the tip of the stalk that is in contact with the receptor chain, HA1, and therefore likely to provide the greatest effect on the motions of the exposed portion of HA. Comparison of this region between virulent strains (1918 H1N1 and H5N1) and less virulent ones (H3N2 and 1930 H1N1) reveals that predicted disorder can be seen at this region among the more virulent strains and subtypes but is remarkably absent among the distinctly less virulent ones.
The motions created by disorder at crucial regions are likely to impair recognition by immunological molecules and increase the virulence of both the H5N1 and the 1918 H1N1 viruses. The results help explain many puzzling features of the H5N1 and the 1918 H1N1 viruses. Summarizing, HA (and especially its intrinsically disordered regions) can serve as a predictor of the influenza A virulence, even though there may be other proteins that contribute to or exacerbate the virulence.
Url:
DOI: 10.1186/1743-422X-6-69
PubMed: 19493338
PubMed Central: 2701943
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000454
Links to Exploration step
PMC:2701943Curation
No country items
Gerard Kian-Meng Goh<affiliation><nlm:aff id="I4">Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore</nlm:aff>
<wicri:noCountry code="subfield">Republic of Singapore</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses</title>
<author><name sortKey="Goh, Gerard Kian Meng" sort="Goh, Gerard Kian Meng" uniqKey="Goh G" first="Gerard Kian-Meng" last="Goh">Gerard Kian-Meng Goh</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="I4">Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore</nlm:aff>
<wicri:noCountry code="subfield">Republic of Singapore</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Dunker, A Keith" sort="Dunker, A Keith" uniqKey="Dunker A" first="A Keith" last="Dunker">A Keith Dunker</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Uversky, Vladimir N" sort="Uversky, Vladimir N" uniqKey="Uversky V" first="Vladimir N" last="Uversky">Vladimir N. Uversky</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Institute for Intrinsically Disordered Protein Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Intrinsically Disordered Protein Research, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Moscow Region</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19493338</idno>
<idno type="pmc">2701943</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701943</idno>
<idno type="RBID">PMC:2701943</idno>
<idno type="doi">10.1186/1743-422X-6-69</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">000454</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000454</idno>
<idno type="wicri:Area/Pmc/Curation">000454</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000454</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses</title>
<author><name sortKey="Goh, Gerard Kian Meng" sort="Goh, Gerard Kian Meng" uniqKey="Goh G" first="Gerard Kian-Meng" last="Goh">Gerard Kian-Meng Goh</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="I4">Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore</nlm:aff>
<wicri:noCountry code="subfield">Republic of Singapore</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Dunker, A Keith" sort="Dunker, A Keith" uniqKey="Dunker A" first="A Keith" last="Dunker">A Keith Dunker</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Uversky, Vladimir N" sort="Uversky, Vladimir N" uniqKey="Uversky V" first="Vladimir N" last="Uversky">Vladimir N. Uversky</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Institute for Intrinsically Disordered Protein Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Intrinsically Disordered Protein Research, Indiana University School of Medicine, Indianapolis, Indiana 46202</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Moscow Region</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Virology Journal</title>
<idno type="eISSN">1743-422X</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>Intrinsic disorder predictor PONDR<sup>® </sup>
VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.</p>
</sec>
<sec><title>Results</title>
<p>The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA<sub>2</sub>
. This region represents the tip of the stalk that is in contact with the receptor chain, HA<sub>1</sub>
, and therefore likely to provide the greatest effect on the motions of the exposed portion of HA. Comparison of this region between virulent strains (1918 H1N1 and H5N1) and less virulent ones (H3N2 and 1930 H1N1) reveals that predicted disorder can be seen at this region among the more virulent strains and subtypes but is remarkably absent among the distinctly less virulent ones.</p>
</sec>
<sec><title>Conclusion</title>
<p>The motions created by disorder at crucial regions are likely to impair recognition by immunological molecules and increase the virulence of both the H5N1 and the 1918 H1N1 viruses. The results help explain many puzzling features of the H5N1 and the 1918 H1N1 viruses. Summarizing, HA (and especially its intrinsically disordered regions) can serve as a predictor of the influenza A virulence, even though there may be other proteins that contribute to or exacerbate the virulence.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virol J</journal-id>
<journal-title>Virology Journal</journal-title>
<issn pub-type="epub">1743-422X</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19493338</article-id>
<article-id pub-id-type="pmc">2701943</article-id>
<article-id pub-id-type="publisher-id">1743-422X-6-69</article-id>
<article-id pub-id-type="doi">10.1186/1743-422X-6-69</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses</article-title>
</title-group>
<contrib-group><contrib id="A1" corresp="yes" contrib-type="author"><name><surname>Goh</surname>
<given-names>Gerard Kian-Meng</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<xref ref-type="aff" rid="I4">4</xref>
<email>gerard@compbio.iupui.edu</email>
</contrib>
<contrib id="A2" contrib-type="author"><name><surname>Dunker</surname>
<given-names>A Keith</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>kedunker@iupui.edu</email>
</contrib>
<contrib id="A3" corresp="yes" contrib-type="author"><name><surname>Uversky</surname>
<given-names>Vladimir N</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<xref ref-type="aff" rid="I2">2</xref>
<xref ref-type="aff" rid="I3">3</xref>
<email>vuversky@iupui.edu</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</aff>
<aff id="I2"><label>2</label>
Institute for Intrinsically Disordered Protein Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA</aff>
<aff id="I3"><label>3</label>
Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia</aff>
<aff id="I4"><label>4</label>
Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore</aff>
<pub-date pub-type="collection"><year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>3</day>
<month>6</month>
<year>2009</year>
</pub-date>
<volume>6</volume>
<fpage>69</fpage>
<lpage>69</lpage>
<ext-link ext-link-type="uri" xlink:href="http://www.virologyj.com/content/6/1/69"></ext-link>
<history><date date-type="received"><day>25</day>
<month>3</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>3</day>
<month>6</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2009 Goh et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2009</copyright-year>
<copyright-holder>Goh et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
<pmc-comment>
Goh
Kian-Meng
Gerard
gerard@compbio.iupui.edu
Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses
2009 Virology Journal 6(1): 69-. (2009) 1743-422X(2009)6:1<69> urn:ISSN:1743-422X </pmc-comment>
</license>
</permissions>
<abstract><sec><title>Background</title>
<p>The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>Intrinsic disorder predictor PONDR<sup>® </sup>
VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.</p>
</sec>
<sec><title>Results</title>
<p>The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA<sub>2</sub>
. This region represents the tip of the stalk that is in contact with the receptor chain, HA<sub>1</sub>
, and therefore likely to provide the greatest effect on the motions of the exposed portion of HA. Comparison of this region between virulent strains (1918 H1N1 and H5N1) and less virulent ones (H3N2 and 1930 H1N1) reveals that predicted disorder can be seen at this region among the more virulent strains and subtypes but is remarkably absent among the distinctly less virulent ones.</p>
</sec>
<sec><title>Conclusion</title>
<p>The motions created by disorder at crucial regions are likely to impair recognition by immunological molecules and increase the virulence of both the H5N1 and the 1918 H1N1 viruses. The results help explain many puzzling features of the H5N1 and the 1918 H1N1 viruses. Summarizing, HA (and especially its intrinsically disordered regions) can serve as a predictor of the influenza A virulence, even though there may be other proteins that contribute to or exacerbate the virulence.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000454 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000454 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:2701943 |texte= Protein intrinsic disorder and influenza virulence: the 1918 H1N1 and H5N1 viruses }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:19493338" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |