Comparative Studies of Wild-Type and “Cold-Mutant” (Temperature-Sensitive) Influenza Viruses: Polypeptide Synthesis by an Asian (H2N2) Strain and Its Cold-Adapted Variant
Identifieur interne : 000406 ( Pmc/Curation ); précédent : 000405; suivant : 000407Comparative Studies of Wild-Type and “Cold-Mutant” (Temperature-Sensitive) Influenza Viruses: Polypeptide Synthesis by an Asian (H2N2) Strain and Its Cold-Adapted Variant
Auteurs : Alan P. Kendal ; Michael P. Kiley ; H. F. MaassabSource :
- Journal of Virology [ 0022-538X ] ; 1973.
Abstract
The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,000-fold less than at 35 C, in contrast to WT virus. Major structural polypeptides of each virus grown at 35 C were similar, except that the hemagglutinin glycopolypeptide (HA) of the TS virions was slightly more heterogenous than that of WT virions. Synthesis of viral polypeptides was examined by sodium dodecyl sulfate acrylamide gel electrophoresis of pulse-labeled infected cells. This revealed a defect in the synthesis of TS viral hemagglutinin that was most pronounced at the nonpermissive temperature. Other TS viral polypeptides appeared to be synthesized normally at 40 C. A defect in the TS virus hemagglutinin was also indicated by serological studies that demonstrated that TS virus hemagglutinin had lost antigenic sites present on the WT virus. Thus, it is concluded that the virus mutant examined contains lesions in the hemagglutinin gene, although the possibility of additional unrecognized lesions is not excluded.
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PubMed: 4796900
PubMed Central: 356793
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative Studies of Wild-Type and “Cold-Mutant” (Temperature-Sensitive) Influenza Viruses: Polypeptide Synthesis by an Asian (H2N2) Strain and Its Cold-Adapted Variant</title><author><name sortKey="Kendal, Alan P" sort="Kendal, Alan P" uniqKey="Kendal A" first="Alan P." last="Kendal">Alan P. Kendal</name></author><author><name sortKey="Kiley, Michael P" sort="Kiley, Michael P" uniqKey="Kiley M" first="Michael P." last="Kiley">Michael P. Kiley</name></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H. F." last="Maassab">H. F. Maassab</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">4796900</idno><idno type="pmc">356793</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC356793</idno><idno type="RBID">PMC:356793</idno><date when="1973">1973</date><idno type="wicri:Area/Pmc/Corpus">000406</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000406</idno><idno type="wicri:Area/Pmc/Curation">000406</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000406</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparative Studies of Wild-Type and “Cold-Mutant” (Temperature-Sensitive) Influenza Viruses: Polypeptide Synthesis by an Asian (H2N2) Strain and Its Cold-Adapted Variant</title><author><name sortKey="Kendal, Alan P" sort="Kendal, Alan P" uniqKey="Kendal A" first="Alan P." last="Kendal">Alan P. Kendal</name></author><author><name sortKey="Kiley, Michael P" sort="Kiley, Michael P" uniqKey="Kiley M" first="Michael P." last="Kiley">Michael P. Kiley</name></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H. F." last="Maassab">H. F. Maassab</name></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="1973">1973</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,000-fold less than at 35 C, in contrast to WT virus. Major structural polypeptides of each virus grown at 35 C were similar, except that the hemagglutinin glycopolypeptide (HA) of the TS virions was slightly more heterogenous than that of WT virions. Synthesis of viral polypeptides was examined by sodium dodecyl sulfate acrylamide gel electrophoresis of pulse-labeled infected cells. This revealed a defect in the synthesis of TS viral hemagglutinin that was most pronounced at the nonpermissive temperature. Other TS viral polypeptides appeared to be synthesized normally at 40 C. A defect in the TS virus hemagglutinin was also indicated by serological studies that demonstrated that TS virus hemagglutinin had lost antigenic sites present on the WT virus. Thus, it is concluded that the virus mutant examined contains lesions in the hemagglutinin gene, although the possibility of additional unrecognized lesions is not excluded.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn></journal-meta><article-meta><article-id pub-id-type="pmid">4796900</article-id><article-id pub-id-type="pmc">356793</article-id><article-categories><subj-group subj-group-type="heading"><subject>Animal Viruses</subject></subj-group></article-categories><title-group><article-title>Comparative Studies of Wild-Type and “Cold-Mutant” (Temperature-Sensitive) Influenza Viruses: Polypeptide Synthesis by an Asian (H2N2) Strain and Its Cold-Adapted Variant</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kendal</surname><given-names>Alan P.</given-names></name><xref ref-type="author-notes" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kiley</surname><given-names>Michael P.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Maassab</surname><given-names>H. F.</given-names></name></contrib></contrib-group><aff id="af1"><label>a</label>Department of Epidemiology, School of Public Health, Ann Arbor, Michigan 48104</aff><author-notes><fn id="au1"><label>1</label><p> Present address: Department of Biological Sciences, University of Maryland Baltimore County, 5401 Wilkens Ave., Catonsville, Md. 21228.</p></fn></author-notes><pub-date pub-type="ppub"><month>12</month><year>1973</year></pub-date><volume>12</volume><issue>6</issue><fpage>1503</fpage><lpage>1511</lpage><copyright-statement>Copyright © 1973 American Society for Microbiology</copyright-statement><abstract><p>The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,000-fold less than at 35 C, in contrast to WT virus. Major structural polypeptides of each virus grown at 35 C were similar, except that the hemagglutinin glycopolypeptide (HA) of the TS virions was slightly more heterogenous than that of WT virions. Synthesis of viral polypeptides was examined by sodium dodecyl sulfate acrylamide gel electrophoresis of pulse-labeled infected cells. This revealed a defect in the synthesis of TS viral hemagglutinin that was most pronounced at the nonpermissive temperature. Other TS viral polypeptides appeared to be synthesized normally at 40 C. A defect in the TS virus hemagglutinin was also indicated by serological studies that demonstrated that TS virus hemagglutinin had lost antigenic sites present on the WT virus. Thus, it is concluded that the virus mutant examined contains lesions in the hemagglutinin gene, although the possibility of additional unrecognized lesions is not excluded.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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