Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs
Identifieur interne : 000034 ( Pmc/Curation ); précédent : 000033; suivant : 000035Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs
Auteurs : Callie Ridenour [États-Unis] ; Adam Johnson [États-Unis] ; Emily Winne [États-Unis] ; Jaber Hossain [États-Unis] ; Guaniri Mateu-Petit [États-Unis] ; Amanda Balish [États-Unis] ; Wanda Santana [États-Unis] ; Taejoong Kim [États-Unis] ; Charles Davis [États-Unis] ; Nancy J. Cox [États-Unis] ; John R. Barr [États-Unis] ; Ruben O. Donis [États-Unis] ; Julie Villanueva [États-Unis] ; Tracie L. Williams [États-Unis] ; Li-Mei Chen [États-Unis]Source :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2015.
Abstract
The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.
Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.
Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.
If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.
Url:
DOI: 10.1111/irv.12322
PubMed: 25962412
PubMed Central: 4548996
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Cox</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Barr, John R" sort="Barr, John R" uniqKey="Barr J" first="John R" last="Barr">John R. Barr</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Williams, Tracie L" sort="Williams, Tracie L" uniqKey="Williams T" first="Tracie L" last="Williams">Tracie L. 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type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548996</idno><idno type="RBID">PMC:4548996</idno><idno type="doi">10.1111/irv.12322</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000034</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000034</idno><idno type="wicri:Area/Pmc/Curation">000034</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000034</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs</title><author><name sortKey="Ridenour, Callie" sort="Ridenour, Callie" uniqKey="Ridenour C" first="Callie" last="Ridenour">Callie Ridenour</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Johnson, Adam" sort="Johnson, Adam" uniqKey="Johnson A" first="Adam" last="Johnson">Adam Johnson</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Winne, Emily" sort="Winne, Emily" uniqKey="Winne E" first="Emily" last="Winne">Emily Winne</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au3"><institution>Battelle Memorial Institute</institution><addr-line>Atlanta, Georgia, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, Georgia</wicri:regionArea></affiliation></author><author><name sortKey="Hossain, Jaber" sort="Hossain, Jaber" uniqKey="Hossain J" first="Jaber" last="Hossain">Jaber Hossain</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Mateu Petit, Guaniri" sort="Mateu Petit, Guaniri" uniqKey="Mateu Petit G" first="Guaniri" last="Mateu-Petit">Guaniri Mateu-Petit</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Balish, Amanda" sort="Balish, Amanda" uniqKey="Balish A" first="Amanda" last="Balish">Amanda Balish</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Santana, Wanda" sort="Santana, Wanda" uniqKey="Santana W" first="Wanda" last="Santana">Wanda Santana</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Taejoong" sort="Kim, Taejoong" uniqKey="Kim T" first="Taejoong" last="Kim">Taejoong Kim</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Davis, Charles" sort="Davis, Charles" uniqKey="Davis C" first="Charles" last="Davis">Charles Davis</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Barr, John R" sort="Barr, John R" uniqKey="Barr J" first="John R" last="Barr">John R. Barr</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Williams, Tracie L" sort="Williams, Tracie L" uniqKey="Williams T" first="Tracie L" last="Williams">Tracie L. Williams</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Atlanta, GA</wicri:regionArea></affiliation></author></analytic><series><title level="j">Influenza and Other Respiratory Viruses</title><idno type="ISSN">1750-2640</idno><idno type="eISSN">1750-2659</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.</p></sec><sec><title>Methods</title><p>Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.</p></sec><sec><title>Results</title><p>Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.</p></sec><sec><title>Conclusions</title><p>If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Liu, D" uniqKey="Liu D">D Liu</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name></author><author><name sortKey="Schulman, Jl" uniqKey="Schulman J">JL Schulman</name></author><author><name sortKey="Schild, Gc" uniqKey="Schild G">GC Schild</name></author><author><name sortKey="Schloer, G" uniqKey="Schloer G">G 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="iso-abbrev">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="publisher-id">irv</journal-id><journal-title-group><journal-title>Influenza and Other Respiratory Viruses</journal-title></journal-title-group><issn pub-type="ppub">1750-2640</issn><issn pub-type="epub">1750-2659</issn><publisher><publisher-name>John Wiley & Sons, Ltd</publisher-name><publisher-loc>Chichester, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25962412</article-id><article-id pub-id-type="pmc">4548996</article-id><article-id pub-id-type="doi">10.1111/irv.12322</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ridenour</surname><given-names>Callie</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Johnson</surname><given-names>Adam</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Winne</surname><given-names>Emily</given-names></name><xref ref-type="aff" rid="au2">b</xref><xref ref-type="aff" rid="au3">c</xref></contrib><contrib contrib-type="author"><name><surname>Hossain</surname><given-names>Jaber</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Mateu-Petit</surname><given-names>Guaniri</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Balish</surname><given-names>Amanda</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Santana</surname><given-names>Wanda</given-names></name><xref ref-type="aff" rid="au2">b</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Taejoong</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Davis</surname><given-names>Charles</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Cox</surname><given-names>Nancy J</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Barr</surname><given-names>John R</given-names></name><xref ref-type="aff" rid="au2">b</xref></contrib><contrib contrib-type="author"><name><surname>Donis</surname><given-names>Ruben O</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Villanueva</surname><given-names>Julie</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Williams</surname><given-names>Tracie L</given-names></name><xref ref-type="aff" rid="au2">b</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Li-Mei</given-names></name><xref ref-type="aff" rid="au1">a</xref><xref ref-type="corresp" rid="cor1"></xref></contrib><aff id="au1"><label>a</label><institution>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></aff><aff id="au2"><label>b</label><institution>Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention</institution><addr-line>Atlanta, GA, USA</addr-line></aff><aff id="au3"><label>c</label><institution>Battelle Memorial Institute</institution><addr-line>Atlanta, Georgia, USA</addr-line></aff></contrib-group><author-notes><corresp id="cor1"><italic>Correspondence:</italic> Li-Mei Chen, Influenza Division, MS-G16, Centers for Disease Control and Prevention, 1600 Atlanta, GA 30333, USA., E-mail: <email>Lchen1@cdc.gov</email></corresp></author-notes><pub-date pub-type="ppub"><month>9</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>04</day><month>8</month><year>2015</year></pub-date><volume>9</volume><issue>5</issue><fpage>263</fpage><lpage>270</lpage><history><date date-type="accepted"><day>30</day><month>4</month><year>2015</year></date></history><permissions><copyright-statement>Published 2015. This article is a U.S. Government work and is in the public domain in the USA.</copyright-statement><copyright-year>2015</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec><title>Background</title><p>The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.</p></sec><sec><title>Methods</title><p>Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.</p></sec><sec><title>Results</title><p>Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.</p></sec><sec><title>Conclusions</title><p>If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.</p></sec></abstract><kwd-group><kwd>Antigen yield</kwd><kwd>H7N9</kwd><kwd>hemagglutinin</kwd><kwd>influenza</kwd><kwd>serial passage</kwd><kwd>vaccine</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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