Reverse Genetics Approaches for the Development of Influenza Vaccines
Identifieur interne : 000D76 ( Pmc/Corpus ); précédent : 000D75; suivant : 000D77Reverse Genetics Approaches for the Development of Influenza Vaccines
Auteurs : Aitor Nogales ; Luis Martínez-SobridoSource :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2016.
Abstract
Influenza viruses cause annual seasonal epidemics and occasional pandemics of human respiratory disease. Influenza virus infections represent a serious public health and economic problem, which are most effectively prevented through vaccination. However, influenza viruses undergo continual antigenic variation, which requires either the annual reformulation of seasonal influenza vaccines or the rapid generation of vaccines against potential pandemic virus strains. The segmented nature of influenza virus allows for the reassortment between two or more viruses within a co-infected cell, and this characteristic has also been harnessed in the laboratory to generate reassortant viruses for their use as either inactivated or live-attenuated influenza vaccines. With the implementation of plasmid-based reverse genetics techniques, it is now possible to engineer recombinant influenza viruses entirely from full-length complementary DNA copies of the viral genome by transfection of susceptible cells. These reverse genetics systems have provided investigators with novel and powerful approaches to answer important questions about the biology of influenza viruses, including the function of viral proteins, their interaction with cellular host factors and the mechanisms of influenza virus transmission and pathogenesis. In addition, reverse genetics techniques have allowed the generation of recombinant influenza viruses, providing a powerful technology to develop both inactivated and live-attenuated influenza vaccines. In this review, we will summarize the current knowledge of state-of-the-art, plasmid-based, influenza reverse genetics approaches and their implementation to provide rapid, convenient, safe and more effective influenza inactivated or live-attenuated vaccines.
Url:
DOI: 10.3390/ijms18010020
PubMed: 28025504
PubMed Central: 5297655
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PMC:5297655Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Reverse Genetics Approaches for the Development of Influenza Vaccines</title><author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name></author><author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28025504</idno><idno type="pmc">5297655</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297655</idno><idno type="RBID">PMC:5297655</idno><idno type="doi">10.3390/ijms18010020</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000D76</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D76</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Reverse Genetics Approaches for the Development of Influenza Vaccines</title><author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name></author><author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Influenza viruses cause annual seasonal epidemics and occasional pandemics of human respiratory disease. Influenza virus infections represent a serious public health and economic problem, which are most effectively prevented through vaccination. However, influenza viruses undergo continual antigenic variation, which requires either the annual reformulation of seasonal influenza vaccines or the rapid generation of vaccines against potential pandemic virus strains. The segmented nature of influenza virus allows for the reassortment between two or more viruses within a co-infected cell, and this characteristic has also been harnessed in the laboratory to generate reassortant viruses for their use as either inactivated or live-attenuated influenza vaccines. With the implementation of plasmid-based reverse genetics techniques, it is now possible to engineer recombinant influenza viruses entirely from full-length complementary DNA copies of the viral genome by transfection of susceptible cells. These reverse genetics systems have provided investigators with novel and powerful approaches to answer important questions about the biology of influenza viruses, including the function of viral proteins, their interaction with cellular host factors and the mechanisms of influenza virus transmission and pathogenesis. In addition, reverse genetics techniques have allowed the generation of recombinant influenza viruses, providing a powerful technology to develop both inactivated and live-attenuated influenza vaccines. In this review, we will summarize the current knowledge of state-of-the-art, plasmid-based, influenza reverse genetics approaches and their implementation to provide rapid, convenient, safe and more effective influenza inactivated or live-attenuated vaccines.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Palese, P S" uniqKey="Palese P">P.S. Palese</name></author><author><name sortKey="Shaw, M L" uniqKey="Shaw M">M.L. Shaw</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steel, J" uniqKey="Steel J">J. Steel</name></author><author><name sortKey="Lowen, A C" uniqKey="Lowen A">A.C. Lowen</name></author><author><name sortKey="Mubareka, S" uniqKey="Mubareka S">S. Mubareka</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parrish, C R" uniqKey="Parrish C">C.R. Parrish</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bean, W J" uniqKey="Bean W">W.J. Bean</name></author><author><name sortKey="Schell, M" uniqKey="Schell M">M. Schell</name></author><author><name sortKey="Katz, J" uniqKey="Katz J">J. Katz</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author><author><name sortKey="Naeve, C" uniqKey="Naeve C">C. Naeve</name></author><author><name sortKey="Gorman, O" uniqKey="Gorman O">O. Gorman</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, R" uniqKey="Chen R">R. Chen</name></author><author><name sortKey="Holmes, E C" uniqKey="Holmes E">E.C. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Rimmelzwaan, G F" uniqKey="Rimmelzwaan G">G.F. Rimmelzwaan</name></author><author><name sortKey="Martina, B E" uniqKey="Martina B">B.E. Martina</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Resa Infante, P" uniqKey="Resa Infante P">P. Resa-Infante</name></author><author><name sortKey="Jorba, N" uniqKey="Jorba N">N. Jorba</name></author><author><name sortKey="Coloma, R" uniqKey="Coloma R">R. Coloma</name></author><author><name sortKey="Ortin, J" uniqKey="Ortin J">J. Ortin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Brownlee, G G" uniqKey="Brownlee G">G.G. Brownlee</name></author><author><name sortKey="Fodor, E" uniqKey="Fodor E">E. Fodor</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, T S" uniqKey="Huang T">T.S. Huang</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author><author><name sortKey="Krystal, M" uniqKey="Krystal M">M. Krystal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wright, P" uniqKey="Wright P">P. Wright</name></author><author><name sortKey="Nuemann, G" uniqKey="Nuemann G">G. Nuemann</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stoeckle, M Y" uniqKey="Stoeckle M">M.Y. Stoeckle</name></author><author><name sortKey="Shaw, M W" uniqKey="Shaw M">M.W. Shaw</name></author><author><name sortKey="Choppin, P W" uniqKey="Choppin P">P.W. Choppin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Finch, C" uniqKey="Finch C">C. Finch</name></author><author><name sortKey="Tuffy, K M" uniqKey="Tuffy K">K.M. Tuffy</name></author><author><name sortKey="Domm, W" uniqKey="Domm W">W. Domm</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Topham, D J" uniqKey="Topham D">D.J. Topham</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackson, D" uniqKey="Jackson D">D. Jackson</name></author><author><name sortKey="Elderfield, R A" uniqKey="Elderfield R">R.A. Elderfield</name></author><author><name sortKey="Barclay, W S" uniqKey="Barclay W">W.S. Barclay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wise, H M" uniqKey="Wise H">H.M. Wise</name></author><author><name sortKey="Hutchinson, E C" uniqKey="Hutchinson E">E.C. Hutchinson</name></author><author><name sortKey="Jagger, B W" uniqKey="Jagger B">B.W. Jagger</name></author><author><name sortKey="Stuart, A D" uniqKey="Stuart A">A.D. Stuart</name></author><author><name sortKey="Kang, Z H" uniqKey="Kang Z">Z.H. Kang</name></author><author><name sortKey="Robb, N" uniqKey="Robb N">N. Robb</name></author><author><name sortKey="Schwartzman, L M" uniqKey="Schwartzman L">L.M. Schwartzman</name></author><author><name sortKey="Kash, J C" uniqKey="Kash J">J.C. Kash</name></author><author><name sortKey="Fodor, E" uniqKey="Fodor E">E. Fodor</name></author><author><name sortKey="Firth, A E" uniqKey="Firth A">A.E. Firth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horvath, C M" uniqKey="Horvath C">C.M. Horvath</name></author><author><name sortKey="Williams, M A" uniqKey="Williams M">M.A. Williams</name></author><author><name sortKey="Lamb, R A" uniqKey="Lamb R">R.A. Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Skehel, J J" uniqKey="Skehel J">J.J. Skehel</name></author><author><name sortKey="Wiley, D C" uniqKey="Wiley D">D.C. Wiley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varghese, J N" uniqKey="Varghese J">J.N. Varghese</name></author><author><name sortKey="Mckimm Breschkin, J L" uniqKey="Mckimm Breschkin J">J.L. McKimm-Breschkin</name></author><author><name sortKey="Caldwell, J B" uniqKey="Caldwell J">J.B. Caldwell</name></author><author><name sortKey="Kortt, A A" uniqKey="Kortt A">A.A. Kortt</name></author><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tulip, W R" uniqKey="Tulip W">W.R. Tulip</name></author><author><name sortKey="Varghese, J N" uniqKey="Varghese J">J.N. Varghese</name></author><author><name sortKey="Laver, W G" uniqKey="Laver W">W.G. Laver</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoon, S W" uniqKey="Yoon S">S.W. Yoon</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tong, S" uniqKey="Tong S">S. Tong</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X. Zhu</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Shi, M" uniqKey="Shi M">M. Shi</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J. Zhang</name></author><author><name sortKey="Bourgeois, M" uniqKey="Bourgeois M">M. Bourgeois</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H. Yang</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X. Chen</name></author><author><name sortKey="Recuenco, S" uniqKey="Recuenco S">S. Recuenco</name></author><author><name sortKey="Gomez, J" uniqKey="Gomez J">J. Gomez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccullers, J A" uniqKey="Mccullers J">J.A. McCullers</name></author><author><name sortKey="Saito, T" uniqKey="Saito T">T. Saito</name></author><author><name sortKey="Iverson, A R" uniqKey="Iverson A">A.R. Iverson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kanegae, Y" uniqKey="Kanegae Y">Y. Kanegae</name></author><author><name sortKey="Sugita, S" uniqKey="Sugita S">S. Sugita</name></author><author><name sortKey="Endo, A" uniqKey="Endo A">A. Endo</name></author><author><name sortKey="Ishida, M" uniqKey="Ishida M">M. Ishida</name></author><author><name sortKey="Senya, S" uniqKey="Senya S">S. Senya</name></author><author><name sortKey="Osako, K" uniqKey="Osako K">K. Osako</name></author><author><name sortKey="Nerome, K" uniqKey="Nerome K">K. Nerome</name></author><author><name sortKey="Oya, A" uniqKey="Oya A">A. Oya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, W W" uniqKey="Thompson W">W.W. Thompson</name></author><author><name sortKey="Shay, D K" uniqKey="Shay D">D.K. Shay</name></author><author><name sortKey="Weintraub, E" uniqKey="Weintraub E">E. Weintraub</name></author><author><name sortKey="Brammer, L" uniqKey="Brammer L">L. Brammer</name></author><author><name sortKey="Cox, N" uniqKey="Cox N">N. Cox</name></author><author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name></author><author><name sortKey="Fukuda, K" uniqKey="Fukuda K">K. Fukuda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W C" uniqKey="Li W">W.C. Li</name></author><author><name sortKey="Shih, S R" uniqKey="Shih S">S.R. Shih</name></author><author><name sortKey="Huang, Y C" uniqKey="Huang Y">Y.C. Huang</name></author><author><name sortKey="Chen, G W" uniqKey="Chen G">G.W. Chen</name></author><author><name sortKey="Chang, S C" uniqKey="Chang S">S.C. Chang</name></author><author><name sortKey="Hsiao, M J" uniqKey="Hsiao M">M.J. Hsiao</name></author><author><name sortKey="Tsao, K C" uniqKey="Tsao K">K.C. Tsao</name></author><author><name sortKey="Lin, T Y" uniqKey="Lin T">T.Y. Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hite, L K" uniqKey="Hite L">L.K. Hite</name></author><author><name sortKey="Glezen, W P" uniqKey="Glezen W">W.P. Glezen</name></author><author><name sortKey="Demmler, G J" uniqKey="Demmler G">G.J. Demmler</name></author><author><name sortKey="Munoz, F M" uniqKey="Munoz F">F.M. Munoz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinto, L H" uniqKey="Pinto L">L.H. Pinto</name></author><author><name sortKey="Holsinger, L J" uniqKey="Holsinger L">L.J. Holsinger</name></author><author><name sortKey="Lamb, R A" uniqKey="Lamb R">R.A. Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lamb, R A" uniqKey="Lamb R">R.A. Lamb</name></author><author><name sortKey="Choppin, P W" uniqKey="Choppin P">P.W. Choppin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hutchinson, E C" uniqKey="Hutchinson E">E.C. Hutchinson</name></author><author><name sortKey="Von Kirchbach, J C" uniqKey="Von Kirchbach J">J.C. von Kirchbach</name></author><author><name sortKey="Gog, J R" uniqKey="Gog J">J.R. Gog</name></author><author><name sortKey="Digard, P" uniqKey="Digard P">P. Digard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rossman, J S" uniqKey="Rossman J">J.S. Rossman</name></author><author><name sortKey="Lamb, R A" uniqKey="Lamb R">R.A. Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gerber, M" uniqKey="Gerber M">M. Gerber</name></author><author><name sortKey="Isel, C" uniqKey="Isel C">C. Isel</name></author><author><name sortKey="Moules, V" uniqKey="Moules V">V. Moules</name></author><author><name sortKey="Marquet, R" uniqKey="Marquet R">R. Marquet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hale, B G" uniqKey="Hale B">B.G. Hale</name></author><author><name sortKey="Randall, R E" uniqKey="Randall R">R.E. Randall</name></author><author><name sortKey="Ortin, J" uniqKey="Ortin J">J. Ortin</name></author><author><name sortKey="Jackson, D" uniqKey="Jackson D">D. Jackson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paterson, D" uniqKey="Paterson D">D. Paterson</name></author><author><name sortKey="Fodor, E" uniqKey="Fodor E">E. Fodor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ampofo, W K" uniqKey="Ampofo W">W.K. Ampofo</name></author><author><name sortKey="Baylor, N" uniqKey="Baylor N">N. Baylor</name></author><author><name sortKey="Cobey, S" uniqKey="Cobey S">S. Cobey</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Daves, S" uniqKey="Daves S">S. Daves</name></author><author><name sortKey="Edwards, S" uniqKey="Edwards S">S. Edwards</name></author><author><name sortKey="Ferguson, N" uniqKey="Ferguson N">N. Ferguson</name></author><author><name sortKey="Grohmann, G" uniqKey="Grohmann G">G. Grohmann</name></author><author><name sortKey="Hay, A" uniqKey="Hay A">A. Hay</name></author><author><name sortKey="Katz, J" uniqKey="Katz J">J. Katz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barr, I G" uniqKey="Barr I">I.G. Barr</name></author><author><name sortKey="Mccauley, J" uniqKey="Mccauley J">J. McCauley</name></author><author><name sortKey="Cox, N" uniqKey="Cox N">N. Cox</name></author><author><name sortKey="Daniels, R" uniqKey="Daniels R">R. Daniels</name></author><author><name sortKey="Engelhardt, O G" uniqKey="Engelhardt O">O.G. Engelhardt</name></author><author><name sortKey="Fukuda, K" uniqKey="Fukuda K">K. Fukuda</name></author><author><name sortKey="Grohmann, G" uniqKey="Grohmann G">G. Grohmann</name></author><author><name sortKey="Hay, A" uniqKey="Hay A">A. Hay</name></author><author><name sortKey="Kelso, A" uniqKey="Kelso A">A. Kelso</name></author><author><name sortKey="Klimov, A" uniqKey="Klimov A">A. Klimov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Brammer, T L" uniqKey="Brammer T">T.L. Brammer</name></author><author><name sortKey="Regnery, H L" uniqKey="Regnery H">H.L. Regnery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kang, S M" uniqKey="Kang S">S.M. Kang</name></author><author><name sortKey="Song, J M" uniqKey="Song J">J.M. Song</name></author><author><name sortKey="Compans, R W" uniqKey="Compans R">R.W. Compans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lynch, J P" uniqKey="Lynch J">J.P. Lynch</name></author><author><name sortKey="Walsh, E E" uniqKey="Walsh E">E.E. Walsh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Molinari, N A" uniqKey="Molinari N">N.A. Molinari</name></author><author><name sortKey="Ortega Sanchez, I R" uniqKey="Ortega Sanchez I">I.R. Ortega-Sanchez</name></author><author><name sortKey="Messonnier, M L" uniqKey="Messonnier M">M.L. Messonnier</name></author><author><name sortKey="Thompson, W W" uniqKey="Thompson W">W.W. Thompson</name></author><author><name sortKey="Wortley, P M" uniqKey="Wortley P">P.M. Wortley</name></author><author><name sortKey="Weintraub, E" uniqKey="Weintraub E">E. Weintraub</name></author><author><name sortKey="Bridges, C B" uniqKey="Bridges C">C.B. Bridges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Girard, M P" uniqKey="Girard M">M.P. Girard</name></author><author><name sortKey="Cherian, T" uniqKey="Cherian T">T. Cherian</name></author><author><name sortKey="Pervikov, Y" uniqKey="Pervikov Y">Y. Pervikov</name></author><author><name sortKey="Kieny, M P" uniqKey="Kieny M">M.P. Kieny</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keech, M" uniqKey="Keech M">M. Keech</name></author><author><name sortKey="Beardsworth, P" uniqKey="Beardsworth P">P. Beardsworth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paul Glezen, W" uniqKey="Paul Glezen W">W. Paul Glezen</name></author><author><name sortKey="Schmier, J K" uniqKey="Schmier J">J.K. Schmier</name></author><author><name sortKey="Kuehn, C M" uniqKey="Kuehn C">C.M. Kuehn</name></author><author><name sortKey="Ryan, K J" uniqKey="Ryan K">K.J. Ryan</name></author><author><name sortKey="Oxford, J" uniqKey="Oxford J">J. Oxford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gasparini, R" uniqKey="Gasparini R">R. Gasparini</name></author><author><name sortKey="Amicizia, D" uniqKey="Amicizia D">D. Amicizia</name></author><author><name sortKey="Lai, P L" uniqKey="Lai P">P.L. Lai</name></author><author><name sortKey="Panatto, D" uniqKey="Panatto D">D. Panatto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, G J" uniqKey="Smith G">G.J. Smith</name></author><author><name sortKey="Vijaykrishna, D" uniqKey="Vijaykrishna D">D. Vijaykrishna</name></author><author><name sortKey="Bahl, J" uniqKey="Bahl J">J. Bahl</name></author><author><name sortKey="Lycett, S J" uniqKey="Lycett S">S.J. Lycett</name></author><author><name sortKey="Worobey, M" uniqKey="Worobey M">M. Worobey</name></author><author><name sortKey="Pybus, O G" uniqKey="Pybus O">O.G. Pybus</name></author><author><name sortKey="Ma, S K" uniqKey="Ma S">S.K. Ma</name></author><author><name sortKey="Cheung, C L" uniqKey="Cheung C">C.L. Cheung</name></author><author><name sortKey="Raghwani, J" uniqKey="Raghwani J">J. Raghwani</name></author><author><name sortKey="Bhatt, S" uniqKey="Bhatt S">S. Bhatt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kilbourne, E D" uniqKey="Kilbourne E">E.D. Kilbourne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carrat, F" uniqKey="Carrat F">F. Carrat</name></author><author><name sortKey="Flahault, A" uniqKey="Flahault A">A. Flahault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X" uniqKey="Xu X">X. Xu</name></author><author><name sortKey="Lindstrom, S E" uniqKey="Lindstrom S">S.E. Lindstrom</name></author><author><name sortKey="Shaw, M W" uniqKey="Shaw M">M.W. Shaw</name></author><author><name sortKey="Smith, C B" uniqKey="Smith C">C.B. Smith</name></author><author><name sortKey="Hall, H E" uniqKey="Hall H">H.E. Hall</name></author><author><name sortKey="Mungall, B A" uniqKey="Mungall B">B.A. Mungall</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. Subbarao</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Klimov, A" uniqKey="Klimov A">A. Klimov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sandbulte, M R" uniqKey="Sandbulte M">M.R. Sandbulte</name></author><author><name sortKey="Westgeest, K B" uniqKey="Westgeest K">K.B. Westgeest</name></author><author><name sortKey="Gao, J" uniqKey="Gao J">J. Gao</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X. Xu</name></author><author><name sortKey="Klimov, A I" uniqKey="Klimov A">A.I. Klimov</name></author><author><name sortKey="Russell, C A" uniqKey="Russell C">C.A. Russell</name></author><author><name sortKey="Burke, D F" uniqKey="Burke D">D.F. Burke</name></author><author><name sortKey="Smith, D J" uniqKey="Smith D">D.J. Smith</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Eichelberger, M C" uniqKey="Eichelberger M">M.C. Eichelberger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shaw, M L" uniqKey="Shaw M">M.L. Shaw</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mikheeva, A" uniqKey="Mikheeva A">A. Mikheeva</name></author><author><name sortKey="Ghendon, Y Z" uniqKey="Ghendon Y">Y.Z. Ghendon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knipe, D M" uniqKey="Knipe D">D.M. Knipe</name></author><author><name sortKey="Howley, P M" uniqKey="Howley P">P.M. Howley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taubenberger, J K" uniqKey="Taubenberger J">J.K. Taubenberger</name></author><author><name sortKey="Morens, D M" uniqKey="Morens D">D.M. Morens</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Uyeki, T M" uniqKey="Uyeki T">T.M. Uyeki</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yen, H L" uniqKey="Yen H">H.L. Yen</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brotherton, J M" uniqKey="Brotherton J">J.M. Brotherton</name></author><author><name sortKey="Delpech, V C" uniqKey="Delpech V">V.C. Delpech</name></author><author><name sortKey="Gilbert, G L" uniqKey="Gilbert G">G.L. Gilbert</name></author><author><name sortKey="Hatzi, S" uniqKey="Hatzi S">S. Hatzi</name></author><author><name sortKey="Paraskevopoulos, P D" uniqKey="Paraskevopoulos P">P.D. Paraskevopoulos</name></author><author><name sortKey="Mcanulty, J M" uniqKey="Mcanulty J">J.M. McAnulty</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Destefano, F" uniqKey="Destefano F">F. DeStefano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fayinka, O A" uniqKey="Fayinka O">O.A. Fayinka</name></author><author><name sortKey="Balayan, M S" uniqKey="Balayan M">M.S. Balayan</name></author><author><name sortKey="Kirya, G B" uniqKey="Kirya G">G.B. Kirya</name></author><author><name sortKey="Rugyendo, W" uniqKey="Rugyendo W">W. Rugyendo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gordon Smith, C E" uniqKey="Gordon Smith C">C.E. Gordon Smith</name></author><author><name sortKey="Thomson, W G" uniqKey="Thomson W">W.G. Thomson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Osterholm, M T" uniqKey="Osterholm M">M.T. Osterholm</name></author><author><name sortKey="Kelley, N S" uniqKey="Kelley N">N.S. Kelley</name></author><author><name sortKey="Sommer, A" uniqKey="Sommer A">A. Sommer</name></author><author><name sortKey="Belongia, E A" uniqKey="Belongia E">E.A. Belongia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Jong, M D" uniqKey="De Jong M">M.D. De Jong</name></author><author><name sortKey="Tran, T T" uniqKey="Tran T">T.T. Tran</name></author><author><name sortKey="Truong, H K" uniqKey="Truong H">H.K. Truong</name></author><author><name sortKey="Vo, M H" uniqKey="Vo M">M.H. Vo</name></author><author><name sortKey="Smith, G J" uniqKey="Smith G">G.J. Smith</name></author><author><name sortKey="Nguyen, V C" uniqKey="Nguyen V">V.C. Nguyen</name></author><author><name sortKey="Bach, V C" uniqKey="Bach V">V.C. Bach</name></author><author><name sortKey="Phan, T Q" uniqKey="Phan T">T.Q. Phan</name></author><author><name sortKey="Do, Q H" uniqKey="Do Q">Q.H. Do</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hurt, A C" uniqKey="Hurt A">A.C. Hurt</name></author><author><name sortKey="Holien, J K" uniqKey="Holien J">J.K. Holien</name></author><author><name sortKey="Parker, M" uniqKey="Parker M">M. Parker</name></author><author><name sortKey="Kelso, A" uniqKey="Kelso A">A. Kelso</name></author><author><name sortKey="Barr, I G" uniqKey="Barr I">I.G. Barr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monto, A S" uniqKey="Monto A">A.S. Monto</name></author><author><name sortKey="Mckimm Breschkin, J L" uniqKey="Mckimm Breschkin J">J.L. McKimm-Breschkin</name></author><author><name sortKey="Macken, C" uniqKey="Macken C">C. Macken</name></author><author><name sortKey="Hampson, A W" uniqKey="Hampson A">A.W. Hampson</name></author><author><name sortKey="Hay, A" uniqKey="Hay A">A. Hay</name></author><author><name sortKey="Klimov, A" uniqKey="Klimov A">A. Klimov</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M. Tashiro</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author><author><name sortKey="Aymard, M" uniqKey="Aymard M">M. Aymard</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belshe, R B" uniqKey="Belshe R">R.B. Belshe</name></author><author><name sortKey="Edwards, K M" uniqKey="Edwards K">K.M. Edwards</name></author><author><name sortKey="Vesikari, T" uniqKey="Vesikari T">T. Vesikari</name></author><author><name sortKey="Black, S V" uniqKey="Black S">S.V. Black</name></author><author><name sortKey="Walker, R E" uniqKey="Walker R">R.E. Walker</name></author><author><name sortKey="Hultquist, M" uniqKey="Hultquist M">M. Hultquist</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author><author><name sortKey="Connor, E M" uniqKey="Connor E">E.M. Connor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belshe, R B" uniqKey="Belshe R">R.B. Belshe</name></author><author><name sortKey="Newman, F K" uniqKey="Newman F">F.K. Newman</name></author><author><name sortKey="Wilkins, K" uniqKey="Wilkins K">K. Wilkins</name></author><author><name sortKey="Graham, I L" uniqKey="Graham I">I.L. Graham</name></author><author><name sortKey="Babusis, E" uniqKey="Babusis E">E. Babusis</name></author><author><name sortKey="Ewell, M" uniqKey="Ewell M">M. Ewell</name></author><author><name sortKey="Frey, S E" uniqKey="Frey S">S.E. Frey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pronker, E S" uniqKey="Pronker E">E.S. Pronker</name></author><author><name sortKey="Claassen, E" uniqKey="Claassen E">E. Claassen</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cox, M M" uniqKey="Cox M">M.M. Cox</name></author><author><name sortKey="Patriarca, P A" uniqKey="Patriarca P">P.A. Patriarca</name></author><author><name sortKey="Treanor, J" uniqKey="Treanor J">J. Treanor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Guo, H" uniqKey="Guo H">H. Guo</name></author><author><name sortKey="Albrecht, R A" uniqKey="Albrecht R">R.A. Albrecht</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Topham, D J" uniqKey="Topham D">D.J. Topham</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, W" uniqKey="Sun W">W. Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belongia, E A" uniqKey="Belongia E">E.A. Belongia</name></author><author><name sortKey="Kieke, B A" uniqKey="Kieke B">B.A. Kieke</name></author><author><name sortKey="Donahue, J G" uniqKey="Donahue J">J.G. Donahue</name></author><author><name sortKey="Greenlee, R T" uniqKey="Greenlee R">R.T. Greenlee</name></author><author><name sortKey="Balish, A" uniqKey="Balish A">A. Balish</name></author><author><name sortKey="Foust, A" uniqKey="Foust A">A. Foust</name></author><author><name sortKey="Lindstrom, S" uniqKey="Lindstrom S">S. Lindstrom</name></author><author><name sortKey="Shay, D K" uniqKey="Shay D">D.K. Shay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pica, N" uniqKey="Pica N">N. Pica</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorse, G J" uniqKey="Gorse G">G.J. Gorse</name></author><author><name sortKey="Belshe, R B" uniqKey="Belshe R">R.B. Belshe</name></author><author><name sortKey="Munn, N J" uniqKey="Munn N">N.J. Munn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oxford, J S" uniqKey="Oxford J">J.S. Oxford</name></author><author><name sortKey="Bossuyt, S" uniqKey="Bossuyt S">S. Bossuyt</name></author><author><name sortKey="Balasingam, S" uniqKey="Balasingam S">S. Balasingam</name></author><author><name sortKey="Mann, A" uniqKey="Mann A">A. Mann</name></author><author><name sortKey="Novelli, P" uniqKey="Novelli P">P. Novelli</name></author><author><name sortKey="Lambkin, R" uniqKey="Lambkin R">R. Lambkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seibert, C W" uniqKey="Seibert C">C.W. Seibert</name></author><author><name sortKey="Kaminski, M" uniqKey="Kaminski M">M. Kaminski</name></author><author><name sortKey="Philipp, J" uniqKey="Philipp J">J. Philipp</name></author><author><name sortKey="Rubbenstroth, D" uniqKey="Rubbenstroth D">D. Rubbenstroth</name></author><author><name sortKey="Albrecht, R A" uniqKey="Albrecht R">R.A. Albrecht</name></author><author><name sortKey="Schwalm, F" uniqKey="Schwalm F">F. Schwalm</name></author><author><name sortKey="Stertz, S" uniqKey="Stertz S">S. Stertz</name></author><author><name sortKey="Medina, R A" uniqKey="Medina R">R.A. Medina</name></author><author><name sortKey="Kochs, G" uniqKey="Kochs G">G. Kochs</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackson, R J" uniqKey="Jackson R">R.J. Jackson</name></author><author><name sortKey="Cooper, K L" uniqKey="Cooper K">K.L. Cooper</name></author><author><name sortKey="Tappenden, P" uniqKey="Tappenden P">P. Tappenden</name></author><author><name sortKey="Rees, A" uniqKey="Rees A">A. Rees</name></author><author><name sortKey="Simpson, E L" uniqKey="Simpson E">E.L. Simpson</name></author><author><name sortKey="Read, R C" uniqKey="Read R">R.C. Read</name></author><author><name sortKey="Nicholson, K G" uniqKey="Nicholson K">K.G. Nicholson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nguyen, J T" uniqKey="Nguyen J">J.T. Nguyen</name></author><author><name sortKey="Hoopes, J D" uniqKey="Hoopes J">J.D. Hoopes</name></author><author><name sortKey="Le, M H" uniqKey="Le M">M.H. Le</name></author><author><name sortKey="Smee, D F" uniqKey="Smee D">D.F. Smee</name></author><author><name sortKey="Patick, A K" uniqKey="Patick A">A.K. Patick</name></author><author><name sortKey="Faix, D J" uniqKey="Faix D">D.J. Faix</name></author><author><name sortKey="Blair, P J" uniqKey="Blair P">P.J. Blair</name></author><author><name sortKey="De Jong, M D" uniqKey="De Jong M">M.D. de Jong</name></author><author><name sortKey="Prichard, M N" uniqKey="Prichard M">M.N. Prichard</name></author><author><name sortKey="Went, G T" uniqKey="Went G">G.T. Went</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hay, A J" uniqKey="Hay A">A.J. Hay</name></author><author><name sortKey="Wolstenholme, A J" uniqKey="Wolstenholme A">A.J. Wolstenholme</name></author><author><name sortKey="Skehel, J J" uniqKey="Skehel J">J.J. Skehel</name></author><author><name sortKey="Smith, M H" uniqKey="Smith M">M.H. Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubareva, L V" uniqKey="Gubareva L">L.V. Gubareva</name></author><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L. Kaiser</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burnham, A J" uniqKey="Burnham A">A.J. Burnham</name></author><author><name sortKey="Baranovich, T" uniqKey="Baranovich T">T. Baranovich</name></author><author><name sortKey="Govorkova, E A" uniqKey="Govorkova E">E.A. Govorkova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farrukee, R" uniqKey="Farrukee R">R. Farrukee</name></author><author><name sortKey="Mosse, J" uniqKey="Mosse J">J. Mosse</name></author><author><name sortKey="Hurt, A C" uniqKey="Hurt A">A.C. Hurt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pizzorno, A" uniqKey="Pizzorno A">A. Pizzorno</name></author><author><name sortKey="Abed, Y" uniqKey="Abed Y">Y. Abed</name></author><author><name sortKey="Boivin, G" uniqKey="Boivin G">G. Boivin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nguyen, H T" uniqKey="Nguyen H">H.T. Nguyen</name></author><author><name sortKey="Sheu, T G" uniqKey="Sheu T">T.G. Sheu</name></author><author><name sortKey="Mishin, V P" uniqKey="Mishin V">V.P. Mishin</name></author><author><name sortKey="Klimov, A I" uniqKey="Klimov A">A.I. Klimov</name></author><author><name sortKey="Gubareva, L V" uniqKey="Gubareva L">L.V. Gubareva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ampofo, W K" uniqKey="Ampofo W">W.K. Ampofo</name></author><author><name sortKey="Al Busaidy, S" uniqKey="Al Busaidy S">S. Al Busaidy</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Giovanni, M" uniqKey="Giovanni M">M. Giovanni</name></author><author><name sortKey="Hay, A" uniqKey="Hay A">A. Hay</name></author><author><name sortKey="Huang, S" uniqKey="Huang S">S. Huang</name></author><author><name sortKey="Inglis, S" uniqKey="Inglis S">S. Inglis</name></author><author><name sortKey="Katz, J" uniqKey="Katz J">J. Katz</name></author><author><name sortKey="Mokhtari Azad, T" uniqKey="Mokhtari Azad T">T. Mokhtari-Azad</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russell, C A" uniqKey="Russell C">C.A. Russell</name></author><author><name sortKey="Jones, T C" uniqKey="Jones T">T.C. Jones</name></author><author><name sortKey="Barr, I G" uniqKey="Barr I">I.G. Barr</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Garten, R J" uniqKey="Garten R">R.J. Garten</name></author><author><name sortKey="Gregory, V" uniqKey="Gregory V">V. Gregory</name></author><author><name sortKey="Gust, I D" uniqKey="Gust I">I.D. Gust</name></author><author><name sortKey="Hampson, A W" uniqKey="Hampson A">A.W. Hampson</name></author><author><name sortKey="Hay, A J" uniqKey="Hay A">A.J. Hay</name></author><author><name sortKey="Hurt, A C" uniqKey="Hurt A">A.C. Hurt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liang, S" uniqKey="Liang S">S. Liang</name></author><author><name sortKey="Mozdzanowska, K" uniqKey="Mozdzanowska K">K. Mozdzanowska</name></author><author><name sortKey="Palladino, G" uniqKey="Palladino G">G. Palladino</name></author><author><name sortKey="Gerhard, W" uniqKey="Gerhard W">W. Gerhard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Medina, R A" uniqKey="Medina R">R.A. Medina</name></author><author><name sortKey="Manicassamy, B" uniqKey="Manicassamy B">B. Manicassamy</name></author><author><name sortKey="Stertz, S" uniqKey="Stertz S">S. Stertz</name></author><author><name sortKey="Seibert, C W" uniqKey="Seibert C">C.W. Seibert</name></author><author><name sortKey="Hai, R" uniqKey="Hai R">R. Hai</name></author><author><name sortKey="Belshe, R B" uniqKey="Belshe R">R.B. Belshe</name></author><author><name sortKey="Frey, S E" uniqKey="Frey S">S.E. Frey</name></author><author><name sortKey="Basler, C F" uniqKey="Basler C">C.F. Basler</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krammer, F" uniqKey="Krammer F">F. Krammer</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author><author><name sortKey="Steel, J" uniqKey="Steel J">J. Steel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Milian, E" uniqKey="Milian E">E. Milian</name></author><author><name sortKey="Kamen, A A" uniqKey="Kamen A">A.A. Kamen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katz, J M" uniqKey="Katz J">J.M. Katz</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, W J" uniqKey="Meyer W">W.J. Meyer</name></author><author><name sortKey="Wood, J M" uniqKey="Wood J">J.M. Wood</name></author><author><name sortKey="Major, D" uniqKey="Major D">D. Major</name></author><author><name sortKey="Robertson, J S" uniqKey="Robertson J">J.S. Robertson</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author><author><name sortKey="Katz, J M" uniqKey="Katz J">J.M. Katz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ampofo, W K" uniqKey="Ampofo W">W.K. Ampofo</name></author><author><name sortKey="Azziz Baumgartner, E" uniqKey="Azziz Baumgartner E">E. Azziz-Baumgartner</name></author><author><name sortKey="Bashir, U" uniqKey="Bashir U">U. Bashir</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Fasce, R" uniqKey="Fasce R">R. Fasce</name></author><author><name sortKey="Giovanni, M" uniqKey="Giovanni M">M. Giovanni</name></author><author><name sortKey="Grohmann, G" uniqKey="Grohmann G">G. Grohmann</name></author><author><name sortKey="Huang, S" uniqKey="Huang S">S. Huang</name></author><author><name sortKey="Katz, J" uniqKey="Katz J">J. Katz</name></author><author><name sortKey="Mironenko, A" uniqKey="Mironenko A">A. Mironenko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salk, J" uniqKey="Salk J">J. Salk</name></author><author><name sortKey="Salk, D" uniqKey="Salk D">D. Salk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Villiers, P J" uniqKey="De Villiers P">P.J. De Villiers</name></author><author><name sortKey="Steele, A D" uniqKey="Steele A">A.D. Steele</name></author><author><name sortKey="Hiemstra, L A" uniqKey="Hiemstra L">L.A. Hiemstra</name></author><author><name sortKey="Rappaport, R" uniqKey="Rappaport R">R. Rappaport</name></author><author><name sortKey="Dunning, A J" uniqKey="Dunning A">A.J. Dunning</name></author><author><name sortKey="Gruber, W C" uniqKey="Gruber W">W.C. Gruber</name></author><author><name sortKey="Forrest, B D" uniqKey="Forrest B">B.D. Forrest</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tlaxca, J L" uniqKey="Tlaxca J">J.L. Tlaxca</name></author><author><name sortKey="Ellis, S" uniqKey="Ellis S">S. Ellis</name></author><author><name sortKey="Remmele, R L" uniqKey="Remmele R">R.L. Remmele</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laver, W G" uniqKey="Laver W">W.G. Laver</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, S S" uniqKey="Wong S">S.S. Wong</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duxbury, A E" uniqKey="Duxbury A">A.E. Duxbury</name></author><author><name sortKey="Hampson, A W" uniqKey="Hampson A">A.W. Hampson</name></author><author><name sortKey="Sievers, J G" uniqKey="Sievers J">J.G. Sievers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baxter, R" uniqKey="Baxter R">R. Baxter</name></author><author><name sortKey="Patriarca, P A" uniqKey="Patriarca P">P.A. Patriarca</name></author><author><name sortKey="Ensor, K" uniqKey="Ensor K">K. Ensor</name></author><author><name sortKey="Izikson, R" uniqKey="Izikson R">R. Izikson</name></author><author><name sortKey="Goldenthal, K L" uniqKey="Goldenthal K">K.L. Goldenthal</name></author><author><name sortKey="Cox, M M" uniqKey="Cox M">M.M. Cox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, G L" uniqKey="Miller G">G.L. Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author><author><name sortKey="Bryant, M L" uniqKey="Bryant M">M.L. Bryant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snyder, M H" uniqKey="Snyder M">M.H. Snyder</name></author><author><name sortKey="Betts, R F" uniqKey="Betts R">R.F. Betts</name></author><author><name sortKey="Deborde, D" uniqKey="Deborde D">D. DeBorde</name></author><author><name sortKey="Tierney, E L" uniqKey="Tierney E">E.L. Tierney</name></author><author><name sortKey="Clements, M L" uniqKey="Clements M">M.L. Clements</name></author><author><name sortKey="Herrington, D" uniqKey="Herrington D">D. Herrington</name></author><author><name sortKey="Sears, S D" uniqKey="Sears S">S.D. Sears</name></author><author><name sortKey="Dolin, R" uniqKey="Dolin R">R. Dolin</name></author><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author><author><name sortKey="Murphy, B R" uniqKey="Murphy B">B.R. Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Kitame, F" uniqKey="Kitame F">F. Kitame</name></author><author><name sortKey="Kendal, A P" uniqKey="Kendal A">A.P. Kendal</name></author><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author><author><name sortKey="Naeve, C" uniqKey="Naeve C">C. Naeve</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Couch, R B" uniqKey="Couch R">R.B. Couch</name></author><author><name sortKey="Keitel, W A" uniqKey="Keitel W">W.A. Keitel</name></author><author><name sortKey="Cate, T R" uniqKey="Cate T">T.R. Cate</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Aspelund, A" uniqKey="Aspelund A">A. Aspelund</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E. Hoffmann</name></author><author><name sortKey="Mahmood, K" uniqKey="Mahmood K">K. Mahmood</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Yang, C F" uniqKey="Yang C">C.F. Yang</name></author><author><name sortKey="Spaete, J" uniqKey="Spaete J">J. Spaete</name></author><author><name sortKey="Greenberg, H B" uniqKey="Greenberg H">H.B. Greenberg</name></author><author><name sortKey="Herlocher, M L" uniqKey="Herlocher M">M.L. Herlocher</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deborde, D C" uniqKey="Deborde D">D.C. DeBorde</name></author><author><name sortKey="Donabedian, A M" uniqKey="Donabedian A">A.M. Donabedian</name></author><author><name sortKey="Herlocher, M L" uniqKey="Herlocher M">M.L. Herlocher</name></author><author><name sortKey="Naeve, C W" uniqKey="Naeve C">C.W. Naeve</name></author><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donabedian, A M" uniqKey="Donabedian A">A.M. Donabedian</name></author><author><name sortKey="Deborde, D C" uniqKey="Deborde D">D.C. DeBorde</name></author><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, W" uniqKey="Chan W">W. Chan</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B. Lu</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B. Lu</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Ma, C" uniqKey="Ma C">C. Ma</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Yang, C F" uniqKey="Yang C">C.F. Yang</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author><author><name sortKey="Greenberg, H" uniqKey="Greenberg H">H. Greenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maassab, H F" uniqKey="Maassab H">H.F. Maassab</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cox, A" uniqKey="Cox A">A. Cox</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Dewhurst, S" uniqKey="Dewhurst S">S. Dewhurst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenhawt, M J" uniqKey="Greenhawt M">M.J. Greenhawt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belshe, R B" uniqKey="Belshe R">R.B. Belshe</name></author><author><name sortKey="Gruber, W C" uniqKey="Gruber W">W.C. Gruber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghendon, Y Z" uniqKey="Ghendon Y">Y.Z. Ghendon</name></author><author><name sortKey="Polezhaev, F I" uniqKey="Polezhaev F">F.I. Polezhaev</name></author><author><name sortKey="Lisovskaya, K V" uniqKey="Lisovskaya K">K.V. Lisovskaya</name></author><author><name sortKey="Medvedeva, T E" uniqKey="Medvedeva T">T.E. Medvedeva</name></author><author><name sortKey="Alexandrova, G I" uniqKey="Alexandrova G">G.I. Alexandrova</name></author><author><name sortKey="Klimov, A I" uniqKey="Klimov A">A.I. Klimov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghendon, Y Z" uniqKey="Ghendon Y">Y.Z. Ghendon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Isakova Sivak, I" uniqKey="Isakova Sivak I">I. Isakova-Sivak</name></author><author><name sortKey="Chen, L M" uniqKey="Chen L">L.M. Chen</name></author><author><name sortKey="Matsuoka, Y" uniqKey="Matsuoka Y">Y. Matsuoka</name></author><author><name sortKey="Voeten, J T" uniqKey="Voeten J">J.T. Voeten</name></author><author><name sortKey="Kiseleva, I" uniqKey="Kiseleva I">I. Kiseleva</name></author><author><name sortKey="Heldens, J G" uniqKey="Heldens J">J.G. Heldens</name></author><author><name sortKey="Den Bosch, H" uniqKey="Den Bosch H">H. den Bosch</name></author><author><name sortKey="Klimov, A" uniqKey="Klimov A">A. Klimov</name></author><author><name sortKey="Rudenko, L" uniqKey="Rudenko L">L. Rudenko</name></author><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kendal, A P" uniqKey="Kendal A">A.P. Kendal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hussain, A I" uniqKey="Hussain A">A.I. Hussain</name></author><author><name sortKey="Cordeiro, M" uniqKey="Cordeiro M">M. Cordeiro</name></author><author><name sortKey="Sevilla, E" uniqKey="Sevilla E">E. Sevilla</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Suguitan, A L" uniqKey="Suguitan A">A.L. Suguitan</name></author><author><name sortKey="Shambaugh, C" uniqKey="Shambaugh C">C. Shambaugh</name></author><author><name sortKey="Kim, L" uniqKey="Kim L">L. Kim</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pearson, H E" uniqKey="Pearson H">H.E. Pearson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Voyles, C" uniqKey="Voyles C">C. Voyles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stulc, J P" uniqKey="Stulc J">J.P. Stulc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Genzel, Y" uniqKey="Genzel Y">Y. Genzel</name></author><author><name sortKey="Reichl, U" uniqKey="Reichl U">U. Reichl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le Ru, A" uniqKey="Le Ru A">A. Le Ru</name></author><author><name sortKey="Jacob, D" uniqKey="Jacob D">D. Jacob</name></author><author><name sortKey="Transfiguracion, J" uniqKey="Transfiguracion J">J. Transfiguracion</name></author><author><name sortKey="Ansorge, S" uniqKey="Ansorge S">S. Ansorge</name></author><author><name sortKey="Henry, O" uniqKey="Henry O">O. Henry</name></author><author><name sortKey="Kamen, A A" uniqKey="Kamen A">A.A. Kamen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Merten, O W" uniqKey="Merten O">O.W. Merten</name></author><author><name sortKey="Manuguerra, J C" uniqKey="Manuguerra J">J.C. Manuguerra</name></author><author><name sortKey="Hannoun, C" uniqKey="Hannoun C">C. Hannoun</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Merten, O W" uniqKey="Merten O">O.W. Merten</name></author><author><name sortKey="Hannoun, C" uniqKey="Hannoun C">C. Hannoun</name></author><author><name sortKey="Manuguerra, J C" uniqKey="Manuguerra J">J.C. Manuguerra</name></author><author><name sortKey="Ventre, F" uniqKey="Ventre F">F. Ventre</name></author><author><name sortKey="Petres, S" uniqKey="Petres S">S. Petres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Del Giudice, G" uniqKey="Del Giudice G">G. Del Giudice</name></author><author><name sortKey="Rappuoli, R" uniqKey="Rappuoli R">R. Rappuoli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, J" uniqKey="Wu J">J. Wu</name></author><author><name sortKey="Liu, S Z" uniqKey="Liu S">S.Z. Liu</name></author><author><name sortKey="Dong, S S" uniqKey="Dong S">S.S. Dong</name></author><author><name sortKey="Dong, X P" uniqKey="Dong X">X.P. Dong</name></author><author><name sortKey="Zhang, W L" uniqKey="Zhang W">W.L. Zhang</name></author><author><name sortKey="Lu, M" uniqKey="Lu M">M. Lu</name></author><author><name sortKey="Li, C G" uniqKey="Li C">C.G. Li</name></author><author><name sortKey="Zhou, J C" uniqKey="Zhou J">J.C. Zhou</name></author><author><name sortKey="Fang, H H" uniqKey="Fang H">H.H. Fang</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Black, S" uniqKey="Black S">S. Black</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, W H" uniqKey="Chen W">W.H. Chen</name></author><author><name sortKey="Jackson, L A" uniqKey="Jackson L">L.A. Jackson</name></author><author><name sortKey="Edwards, K M" uniqKey="Edwards K">K.M. Edwards</name></author><author><name sortKey="Keitel, W A" uniqKey="Keitel W">W.A. Keitel</name></author><author><name sortKey="Hill, H" uniqKey="Hill H">H. Hill</name></author><author><name sortKey="Noah, D L" uniqKey="Noah D">D.L. Noah</name></author><author><name sortKey="Creech, C B" uniqKey="Creech C">C.B. Creech</name></author><author><name sortKey="Patel, S M" uniqKey="Patel S">S.M. Patel</name></author><author><name sortKey="Mangal, B" uniqKey="Mangal B">B. Mangal</name></author><author><name sortKey="Kotloff, K L" uniqKey="Kotloff K">K.L. Kotloff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stobart, C C" uniqKey="Stobart C">C.C. Stobart</name></author><author><name sortKey="Moore, M L" uniqKey="Moore M">M.L. Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F. Almazan</name></author><author><name sortKey="Marquez Jurado, S" uniqKey="Marquez Jurado S">S. Marquez-Jurado</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F. Almazan</name></author><author><name sortKey="Dediego, M L" uniqKey="Dediego M">M.L. Dediego</name></author><author><name sortKey="Galan, C" uniqKey="Galan C">C. Galan</name></author><author><name sortKey="Escors, D" uniqKey="Escors D">D. Escors</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E. Alvarez</name></author><author><name sortKey="Ortego, J" uniqKey="Ortego J">J. Ortego</name></author><author><name sortKey="Sola, I" uniqKey="Sola I">I. Sola</name></author><author><name sortKey="Zuniga, S" uniqKey="Zuniga S">S. Zuniga</name></author><author><name sortKey="Alonso, S" uniqKey="Alonso S">S. Alonso</name></author><author><name sortKey="Moreno, J L" uniqKey="Moreno J">J.L. Moreno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Racaniello, V R" uniqKey="Racaniello V">V.R. Racaniello</name></author><author><name sortKey="Baltimore, D" uniqKey="Baltimore D">D. Baltimore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engelhardt, O G" uniqKey="Engelhardt O">O.G. Engelhardt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Ortiz Riano, E" uniqKey="Ortiz Riano E">E. Ortiz-Riano</name></author><author><name sortKey="Dewhurst, S" uniqKey="Dewhurst S">S. Dewhurst</name></author><author><name sortKey="Topham, D J" uniqKey="Topham D">D.J. Topham</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Cadagan, R" uniqKey="Cadagan R">R. Cadagan</name></author><author><name sortKey="Steel, J" uniqKey="Steel J">J. Steel</name></author><author><name sortKey="Basler, C F" uniqKey="Basler C">C.F. Basler</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author><author><name sortKey="Moran, T M" uniqKey="Moran T">T.M. Moran</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Domm, W" uniqKey="Domm W">W. Domm</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breen, M" uniqKey="Breen M">M. Breen</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Rodriguez Sanchez, I" uniqKey="Rodriguez Sanchez I">I. Rodriguez-Sanchez</name></author><author><name sortKey="Monte, K" uniqKey="Monte K">K. Monte</name></author><author><name sortKey="Lenschow, D J" uniqKey="Lenschow D">D.J. Lenschow</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breen, M" uniqKey="Breen M">M. Breen</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zobel, A" uniqKey="Zobel A">A. Zobel</name></author><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Hobom, G" uniqKey="Hobom G">G. Hobom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Zobel, A" uniqKey="Zobel A">A. Zobel</name></author><author><name sortKey="Hobom, G" uniqKey="Hobom G">G. Hobom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E. Hoffmann</name></author><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author><author><name sortKey="Hobom, G" uniqKey="Hobom G">G. Hobom</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E. Hoffmann</name></author><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Hobom, G" uniqKey="Hobom G">G. Hobom</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, B Y" uniqKey="Cheng B">B.Y. Cheng</name></author><author><name sortKey="Ortiz Riano, E" uniqKey="Ortiz Riano E">E. Ortiz-Riano</name></author><author><name sortKey="De La Torre, J C" uniqKey="De La Torre J">J.C. de la Torre</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ortiz Riano, E" uniqKey="Ortiz Riano E">E. Ortiz-Riano</name></author><author><name sortKey="Cheng, B Y" uniqKey="Cheng B">B.Y. Cheng</name></author><author><name sortKey="Carlos De La Torre, J" uniqKey="Carlos De La Torre J">J. Carlos de la Torre</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name></author><author><name sortKey="Watanabe, T" uniqKey="Watanabe T">T. Watanabe</name></author><author><name sortKey="Ito, H" uniqKey="Ito H">H. Ito</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S. Watanabe</name></author><author><name sortKey="Goto, H" uniqKey="Goto H">H. Goto</name></author><author><name sortKey="Gao, P" uniqKey="Gao P">P. Gao</name></author><author><name sortKey="Hughes, M" uniqKey="Hughes M">M. Hughes</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Donis, R" uniqKey="Donis R">R. Donis</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E. Hoffmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author><author><name sortKey="Duke, G M" uniqKey="Duke G">G.M. Duke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Massin, P" uniqKey="Massin P">P. Massin</name></author><author><name sortKey="Rodrigues, P" uniqKey="Rodrigues P">P. Rodrigues</name></author><author><name sortKey="Marasescu, M" uniqKey="Marasescu M">M. Marasescu</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name></author><author><name sortKey="Naffakh, N" uniqKey="Naffakh N">N. Naffakh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Curtiss, R" uniqKey="Curtiss R">R. Curtiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="He, D" uniqKey="He D">D. He</name></author><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author><author><name sortKey="Ou, S" uniqKey="Ou S">S. Ou</name></author><author><name sortKey="Yuan, R" uniqKey="Yuan R">R. Yuan</name></author><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fodor, E" uniqKey="Fodor E">E. Fodor</name></author><author><name sortKey="Devenish, L" uniqKey="Devenish L">L. Devenish</name></author><author><name sortKey="Engelhardt, O G" uniqKey="Engelhardt O">O.G. Engelhardt</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author><author><name sortKey="Brownlee, G G" uniqKey="Brownlee G">G.G. Brownlee</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, B" uniqKey="Zhou B">B. Zhou</name></author><author><name sortKey="Lin, X" uniqKey="Lin X">X. Lin</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Halpin, R A" uniqKey="Halpin R">R.A. Halpin</name></author><author><name sortKey="Bera, J" uniqKey="Bera J">J. Bera</name></author><author><name sortKey="Stockwell, T B" uniqKey="Stockwell T">T.B. Stockwell</name></author><author><name sortKey="Barr, I G" uniqKey="Barr I">I.G. Barr</name></author><author><name sortKey="Wentworth, D E" uniqKey="Wentworth D">D.E. Wentworth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackson, D" uniqKey="Jackson D">D. Jackson</name></author><author><name sortKey="Cadman, A" uniqKey="Cadman A">A. Cadman</name></author><author><name sortKey="Zurcher, T" uniqKey="Zurcher T">T. Zurcher</name></author><author><name sortKey="Barclay, W S" uniqKey="Barclay W">W.S. Barclay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E. Hoffmann</name></author><author><name sortKey="Mahmood, K" uniqKey="Mahmood K">K. Mahmood</name></author><author><name sortKey="Yang, C F" uniqKey="Yang C">C.F. Yang</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author><author><name sortKey="Greenberg, H B" uniqKey="Greenberg H">H.B. Greenberg</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ozawa, M" uniqKey="Ozawa M">M. Ozawa</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marsh, G A" uniqKey="Marsh G">G.A. Marsh</name></author><author><name sortKey="Hatami, R" uniqKey="Hatami R">R. Hatami</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Isel, C" uniqKey="Isel C">C. Isel</name></author><author><name sortKey="Munier, S" uniqKey="Munier S">S. Munier</name></author><author><name sortKey="Naffakh, N" uniqKey="Naffakh N">N. Naffakh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schickli, J H" uniqKey="Schickli J">J.H. Schickli</name></author><author><name sortKey="Flandorfer, A" uniqKey="Flandorfer A">A. Flandorfer</name></author><author><name sortKey="Nakaya, T" uniqKey="Nakaya T">T. Nakaya</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steel, J" uniqKey="Steel J">J. Steel</name></author><author><name sortKey="Lowen, A C" uniqKey="Lowen A">A.C. Lowen</name></author><author><name sortKey="Pena, L" uniqKey="Pena L">L. Pena</name></author><author><name sortKey="Angel, M" uniqKey="Angel M">M. Angel</name></author><author><name sortKey="Solorzano, A" uniqKey="Solorzano A">A. Solorzano</name></author><author><name sortKey="Albrecht, R" uniqKey="Albrecht R">R. Albrecht</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Richt, J A" uniqKey="Richt J">J.A. Richt</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vincent, A L" uniqKey="Vincent A">A.L. Vincent</name></author><author><name sortKey="Ma, W" uniqKey="Ma W">W. Ma</name></author><author><name sortKey="Lager, K M" uniqKey="Lager K">K.M. Lager</name></author><author><name sortKey="Janke, B H" uniqKey="Janke B">B.H. Janke</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Richt, J A" uniqKey="Richt J">J.A. Richt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quinlivan, M" uniqKey="Quinlivan M">M. Quinlivan</name></author><author><name sortKey="Zamarin, D" uniqKey="Zamarin D">D. Zamarin</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Cullinane, A" uniqKey="Cullinane A">A. Cullinane</name></author><author><name sortKey="Chambers, T" uniqKey="Chambers T">T. Chambers</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Falcon, A M" uniqKey="Falcon A">A.M. Falcon</name></author><author><name sortKey="Fernandez Sesma, A" uniqKey="Fernandez Sesma A">A. Fernandez-Sesma</name></author><author><name sortKey="Nakaya, Y" uniqKey="Nakaya Y">Y. Nakaya</name></author><author><name sortKey="Moran, T M" uniqKey="Moran T">T.M. Moran</name></author><author><name sortKey="Ortin, J" uniqKey="Ortin J">J. Ortin</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ferko, B" uniqKey="Ferko B">B. Ferko</name></author><author><name sortKey="Stasakova, J" uniqKey="Stasakova J">J. Stasakova</name></author><author><name sortKey="Romanova, J" uniqKey="Romanova J">J. Romanova</name></author><author><name sortKey="Kittel, C" uniqKey="Kittel C">C. Kittel</name></author><author><name sortKey="Sereinig, S" uniqKey="Sereinig S">S. Sereinig</name></author><author><name sortKey="Katinger, H" uniqKey="Katinger H">H. Katinger</name></author><author><name sortKey="Egorov, A" uniqKey="Egorov A">A. Egorov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Richt, J A" uniqKey="Richt J">J.A. Richt</name></author><author><name sortKey="Lekcharoensuk, P" uniqKey="Lekcharoensuk P">P. Lekcharoensuk</name></author><author><name sortKey="Lager, K M" uniqKey="Lager K">K.M. Lager</name></author><author><name sortKey="Vincent, A L" uniqKey="Vincent A">A.L. Vincent</name></author><author><name sortKey="Loiacono, C M" uniqKey="Loiacono C">C.M. Loiacono</name></author><author><name sortKey="Janke, B H" uniqKey="Janke B">B.H. Janke</name></author><author><name sortKey="Wu, W H" uniqKey="Wu W">W.H. Wu</name></author><author><name sortKey="Yoon, K J" uniqKey="Yoon K">K.J. Yoon</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author><author><name sortKey="Solorzano, A" uniqKey="Solorzano A">A. Solorzano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Solorzano, A" uniqKey="Solorzano A">A. Solorzano</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author><author><name sortKey="Lager, K M" uniqKey="Lager K">K.M. Lager</name></author><author><name sortKey="Janke, B H" uniqKey="Janke B">B.H. Janke</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Richt, J A" uniqKey="Richt J">J.A. Richt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Suarez, D L" uniqKey="Suarez D">D.L. Suarez</name></author><author><name sortKey="Pantin Jackwood, M" uniqKey="Pantin Jackwood M">M. Pantin-Jackwood</name></author><author><name sortKey="Mibayashi, M" uniqKey="Mibayashi M">M. Mibayashi</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Saif, Y M" uniqKey="Saif Y">Y.M. Saif</name></author><author><name sortKey="Lee, C W" uniqKey="Lee C">C.W. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Choi, E H" uniqKey="Choi E">E.H. Choi</name></author><author><name sortKey="Song, M S" uniqKey="Song M">M.S. Song</name></author><author><name sortKey="Park, S J" uniqKey="Park S">S.J. Park</name></author><author><name sortKey="Pascua, P N" uniqKey="Pascua P">P.N. Pascua</name></author><author><name sortKey="Baek, Y H" uniqKey="Baek Y">Y.H. Baek</name></author><author><name sortKey="Kwon, H I" uniqKey="Kwon H">H.I. Kwon</name></author><author><name sortKey="Kim, E H" uniqKey="Kim E">E.H. Kim</name></author><author><name sortKey="Kim, S" uniqKey="Kim S">S. Kim</name></author><author><name sortKey="Jang, H K" uniqKey="Jang H">H.K. Jang</name></author><author><name sortKey="Poo, H" uniqKey="Poo H">H. Poo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Huang, K" uniqKey="Huang K">K. Huang</name></author><author><name sortKey="Chauche, C" uniqKey="Chauche C">C. Chauche</name></author><author><name sortKey="Dediego, M L" uniqKey="Dediego M">M.L. DeDiego</name></author><author><name sortKey="Murcia, P R" uniqKey="Murcia P">P.R. Murcia</name></author><author><name sortKey="Parrish, C R" uniqKey="Parrish C">C.R. Parrish</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baskin, C R" uniqKey="Baskin C">C.R. Baskin</name></author><author><name sortKey="Bielefeldt Ohmann, H" uniqKey="Bielefeldt Ohmann H">H. Bielefeldt-Ohmann</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Tumpey, T M" uniqKey="Tumpey T">T.M. Tumpey</name></author><author><name sortKey="Van Hoeven, N" uniqKey="Van Hoeven N">N. van Hoeven</name></author><author><name sortKey="Carter, V S" uniqKey="Carter V">V.S. Carter</name></author><author><name sortKey="Thomas, M J" uniqKey="Thomas M">M.J. Thomas</name></author><author><name sortKey="Proll, S" uniqKey="Proll S">S. Proll</name></author><author><name sortKey="Solorzano, A" uniqKey="Solorzano A">A. Solorzano</name></author><author><name sortKey="Billharz, R" uniqKey="Billharz R">R. Billharz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pica, N" uniqKey="Pica N">N. Pica</name></author><author><name sortKey="Langlois, R A" uniqKey="Langlois R">R.A. Langlois</name></author><author><name sortKey="Krammer, F" uniqKey="Krammer F">F. Krammer</name></author><author><name sortKey="Margine, I" uniqKey="Margine I">I. Margine</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hai, R" uniqKey="Hai R">R. Hai</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Fraser, K A" uniqKey="Fraser K">K.A. Fraser</name></author><author><name sortKey="Ayllon, J" uniqKey="Ayllon J">J. Ayllon</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Talon, J" uniqKey="Talon J">J. Talon</name></author><author><name sortKey="Salvatore, M" uniqKey="Salvatore M">M. Salvatore</name></author><author><name sortKey="O Neill, R E" uniqKey="O Neill R">R.E. O'Neill</name></author><author><name sortKey="Nakaya, Y" uniqKey="Nakaya Y">Y. Nakaya</name></author><author><name sortKey="Zheng, H" uniqKey="Zheng H">H. Zheng</name></author><author><name sortKey="Muster, T" uniqKey="Muster T">T. Muster</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, B Y" uniqKey="Cheng B">B.Y. Cheng</name></author><author><name sortKey="Ortiz Riano, E" uniqKey="Ortiz Riano E">E. Ortiz-Riano</name></author><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="De La Torre, J C" uniqKey="De La Torre J">J.C. de la Torre</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mueller, S" uniqKey="Mueller S">S. Mueller</name></author><author><name sortKey="Coleman, J R" uniqKey="Coleman J">J.R. Coleman</name></author><author><name sortKey="Papamichail, D" uniqKey="Papamichail D">D. Papamichail</name></author><author><name sortKey="Ward, C B" uniqKey="Ward C">C.B. Ward</name></author><author><name sortKey="Nimnual, A" uniqKey="Nimnual A">A. Nimnual</name></author><author><name sortKey="Futcher, B" uniqKey="Futcher B">B. Futcher</name></author><author><name sortKey="Skiena, S" uniqKey="Skiena S">S. Skiena</name></author><author><name sortKey="Wimmer, E" uniqKey="Wimmer E">E. Wimmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, C" uniqKey="Yang C">C. Yang</name></author><author><name sortKey="Skiena, S" uniqKey="Skiena S">S. Skiena</name></author><author><name sortKey="Futcher, B" uniqKey="Futcher B">B. Futcher</name></author><author><name sortKey="Mueller, S" uniqKey="Mueller S">S. Mueller</name></author><author><name sortKey="Wimmer, E" uniqKey="Wimmer E">E. Wimmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katsura, H" uniqKey="Katsura H">H. Katsura</name></author><author><name sortKey="Iwatsuki Horimoto, K" uniqKey="Iwatsuki Horimoto K">K. Iwatsuki-Horimoto</name></author><author><name sortKey="Fukuyama, S" uniqKey="Fukuyama S">S. Fukuyama</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S. Watanabe</name></author><author><name sortKey="Sakabe, S" uniqKey="Sakabe S">S. Sakabe</name></author><author><name sortKey="Hatta, Y" uniqKey="Hatta Y">Y. Hatta</name></author><author><name sortKey="Murakami, S" uniqKey="Murakami S">S. Murakami</name></author><author><name sortKey="Shimojima, M" uniqKey="Shimojima M">M. Shimojima</name></author><author><name sortKey="Horimoto, T" uniqKey="Horimoto T">T. Horimoto</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Powell, T J" uniqKey="Powell T">T.J. Powell</name></author><author><name sortKey="Silk, J D" uniqKey="Silk J">J.D. Silk</name></author><author><name sortKey="Sharps, J" uniqKey="Sharps J">J. Sharps</name></author><author><name sortKey="Fodor, E" uniqKey="Fodor E">E. Fodor</name></author><author><name sortKey="Townsend, A R" uniqKey="Townsend A">A.R. Townsend</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shinya, K" uniqKey="Shinya K">K. Shinya</name></author><author><name sortKey="Fujii, Y" uniqKey="Fujii Y">Y. Fujii</name></author><author><name sortKey="Ito, H" uniqKey="Ito H">H. Ito</name></author><author><name sortKey="Ito, T" uniqKey="Ito T">T. Ito</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uraki, R" uniqKey="Uraki R">R. Uraki</name></author><author><name sortKey="Kiso, M" uniqKey="Kiso M">M. Kiso</name></author><author><name sortKey="Iwatsuki Horimoto, K" uniqKey="Iwatsuki Horimoto K">K. Iwatsuki-Horimoto</name></author><author><name sortKey="Fukuyama, S" uniqKey="Fukuyama S">S. Fukuyama</name></author><author><name sortKey="Takashita, E" uniqKey="Takashita E">E. Takashita</name></author><author><name sortKey="Ozawa, M" uniqKey="Ozawa M">M. Ozawa</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Victor, S T" uniqKey="Victor S">S.T. Victor</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S. Watanabe</name></author><author><name sortKey="Katsura, H" uniqKey="Katsura H">H. Katsura</name></author><author><name sortKey="Ozawa, M" uniqKey="Ozawa M">M. Ozawa</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, H" uniqKey="Guo H">H. Guo</name></author><author><name sortKey="Baker, S F" uniqKey="Baker S">S.F. Baker</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author><author><name sortKey="Topham, D J" uniqKey="Topham D">D.J. Topham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masic, A" uniqKey="Masic A">A. Masic</name></author><author><name sortKey="Pyo, H M" uniqKey="Pyo H">H.M. Pyo</name></author><author><name sortKey="Babiuk, S" uniqKey="Babiuk S">S. Babiuk</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fonseca, W" uniqKey="Fonseca W">W. Fonseca</name></author><author><name sortKey="Ozawa, M" uniqKey="Ozawa M">M. Ozawa</name></author><author><name sortKey="Hatta, M" uniqKey="Hatta M">M. Hatta</name></author><author><name sortKey="Orozco, E" uniqKey="Orozco E">E. Orozco</name></author><author><name sortKey="Martinez, M B" uniqKey="Martinez M">M.B. Martinez</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobayashi, H" uniqKey="Kobayashi H">H. Kobayashi</name></author><author><name sortKey="Iwatsuki Horimoto, K" uniqKey="Iwatsuki Horimoto K">K. Iwatsuki-Horimoto</name></author><author><name sortKey="Kiso, M" uniqKey="Kiso M">M. Kiso</name></author><author><name sortKey="Uraki, R" uniqKey="Uraki R">R. Uraki</name></author><author><name sortKey="Ichiko, Y" uniqKey="Ichiko Y">Y. Ichiko</name></author><author><name sortKey="Takimoto, T" uniqKey="Takimoto T">T. Takimoto</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katsura, H" uniqKey="Katsura H">H. Katsura</name></author><author><name sortKey="Piao, Z" uniqKey="Piao Z">Z. Piao</name></author><author><name sortKey="Iwatsuki Horimoto, K" uniqKey="Iwatsuki Horimoto K">K. Iwatsuki-Horimoto</name></author><author><name sortKey="Akeda, Y" uniqKey="Akeda Y">Y. Akeda</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S. Watanabe</name></author><author><name sortKey="Horimoto, T" uniqKey="Horimoto T">T. Horimoto</name></author><author><name sortKey="Oishi, K" uniqKey="Oishi K">K. Oishi</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pena, L" uniqKey="Pena L">L. Pena</name></author><author><name sortKey="Sutton, T" uniqKey="Sutton T">T. Sutton</name></author><author><name sortKey="Chockalingam, A" uniqKey="Chockalingam A">A. Chockalingam</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author><author><name sortKey="Angel, M" uniqKey="Angel M">M. Angel</name></author><author><name sortKey="Shao, H" uniqKey="Shao H">H. Shao</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, A" uniqKey="Nogales A">A. Nogales</name></author><author><name sortKey="Dediego, M L" uniqKey="Dediego M">M.L. DeDiego</name></author><author><name sortKey="Topham, D J" uniqKey="Topham D">D.J. Topham</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L. Martinez-Sobrido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A. Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perez, D R" uniqKey="Perez D">D.R. Perez</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W. Lim</name></author><author><name sortKey="Seiler, J P" uniqKey="Seiler J">J.P. Seiler</name></author><author><name sortKey="Yi, G" uniqKey="Yi G">G. Yi</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name></author><author><name sortKey="Shortridge, K F" uniqKey="Shortridge K">K.F. Shortridge</name></author><author><name sortKey="Webster, R G" uniqKey="Webster R">R.G. Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deng, L" uniqKey="Deng L">L. Deng</name></author><author><name sortKey="Cho, K J" uniqKey="Cho K">K.J. Cho</name></author><author><name sortKey="Fiers, W" uniqKey="Fiers W">W. Fiers</name></author><author><name sortKey="Saelens, X" uniqKey="Saelens X">X. Saelens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilson, I A" uniqKey="Wilson I">I.A. Wilson</name></author><author><name sortKey="Skehel, J J" uniqKey="Skehel J">J.J. Skehel</name></author><author><name sortKey="Wiley, D C" uniqKey="Wiley D">D.C. Wiley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Compans, R W" uniqKey="Compans R">R.W. Compans</name></author><author><name sortKey="Wang, B Z" uniqKey="Wang B">B.Z. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, H" uniqKey="Song H">H. Song</name></author><author><name sortKey="Wittman, V" uniqKey="Wittman V">V. Wittman</name></author><author><name sortKey="Byers, A" uniqKey="Byers A">A. Byers</name></author><author><name sortKey="Tapia, T" uniqKey="Tapia T">T. Tapia</name></author><author><name sortKey="Zhou, B" uniqKey="Zhou B">B. Zhou</name></author><author><name sortKey="Warren, W" uniqKey="Warren W">W. Warren</name></author><author><name sortKey="Heaton, P" uniqKey="Heaton P">P. Heaton</name></author><author><name sortKey="Connolly, K" uniqKey="Connolly K">K. Connolly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Latham, T" uniqKey="Latham T">T. Latham</name></author><author><name sortKey="Galarza, J M" uniqKey="Galarza J">J.M. Galarza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pushko, P" uniqKey="Pushko P">P. Pushko</name></author><author><name sortKey="Tumpey, T M" uniqKey="Tumpey T">T.M. Tumpey</name></author><author><name sortKey="Bu, F" uniqKey="Bu F">F. Bu</name></author><author><name sortKey="Knell, J" uniqKey="Knell J">J. Knell</name></author><author><name sortKey="Robinson, R" uniqKey="Robinson R">R. Robinson</name></author><author><name sortKey="Smith, G" uniqKey="Smith G">G. Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, B J" uniqKey="Chen B">B.J. Chen</name></author><author><name sortKey="Leser, G P" uniqKey="Leser G">G.P. Leser</name></author><author><name sortKey="Morita, E" uniqKey="Morita E">E. Morita</name></author><author><name sortKey="Lamb, R A" uniqKey="Lamb R">R.A. Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pushko, P" uniqKey="Pushko P">P. Pushko</name></author><author><name sortKey="Pearce, M B" uniqKey="Pearce M">M.B. Pearce</name></author><author><name sortKey="Ahmad, A" uniqKey="Ahmad A">A. Ahmad</name></author><author><name sortKey="Tretyakova, I" uniqKey="Tretyakova I">I. Tretyakova</name></author><author><name sortKey="Smith, G" uniqKey="Smith G">G. Smith</name></author><author><name sortKey="Belser, J A" uniqKey="Belser J">J.A. Belser</name></author><author><name sortKey="Tumpey, T M" uniqKey="Tumpey T">T.M. Tumpey</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="review-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Mol Sci</journal-id><journal-id journal-id-type="iso-abbrev">Int J Mol Sci</journal-id><journal-id journal-id-type="publisher-id">ijms</journal-id><journal-title-group><journal-title>International Journal of Molecular Sciences</journal-title></journal-title-group><issn pub-type="epub">1422-0067</issn><publisher><publisher-name>MDPI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28025504</article-id><article-id pub-id-type="pmc">5297655</article-id><article-id pub-id-type="doi">10.3390/ijms18010020</article-id><article-id pub-id-type="publisher-id">ijms-18-00020</article-id><article-categories><subj-group subj-group-type="heading"><subject>Review</subject></subj-group></article-categories><title-group><article-title>Reverse Genetics Approaches for the Development of Influenza Vaccines</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nogales</surname><given-names>Aitor</given-names></name><xref rid="c1-ijms-18-00020" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Martínez-Sobrido</surname><given-names>Luis</given-names></name><xref rid="c1-ijms-18-00020" ref-type="corresp">*</xref></contrib></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Woelk</surname><given-names>Christopher</given-names></name><role>Academic Editor</role></contrib></contrib-group><aff id="af1-ijms-18-00020">Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA</aff><author-notes><corresp id="c1-ijms-18-00020"><label>*</label>Correspondence: <email>aitor_nogales@urmc.rochester.edu</email> (A.N.); <email>luis_martinez@urmc.rochester.edu</email> (L.M.-S.); Tel.: +1-585-276-4847 (A.N.); +1-585-276-4733 (L.M.-S.)</corresp></author-notes><pub-date pub-type="epub"><day>22</day><month>12</month><year>2016</year></pub-date><pub-date pub-type="collection"><month>1</month><year>2017</year></pub-date><volume>18</volume><issue>1</issue><elocation-id>20</elocation-id><history><date date-type="received"><day>22</day><month>11</month><year>2016</year></date><date date-type="accepted"><day>19</day><month>12</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 by the authors; licensee MDPI, Basel, Switzerland.</copyright-statement><copyright-year>2016</copyright-year><license><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>).</license-p></license></permissions><abstract><p>Influenza viruses cause annual seasonal epidemics and occasional pandemics of human respiratory disease. Influenza virus infections represent a serious public health and economic problem, which are most effectively prevented through vaccination. However, influenza viruses undergo continual antigenic variation, which requires either the annual reformulation of seasonal influenza vaccines or the rapid generation of vaccines against potential pandemic virus strains. The segmented nature of influenza virus allows for the reassortment between two or more viruses within a co-infected cell, and this characteristic has also been harnessed in the laboratory to generate reassortant viruses for their use as either inactivated or live-attenuated influenza vaccines. With the implementation of plasmid-based reverse genetics techniques, it is now possible to engineer recombinant influenza viruses entirely from full-length complementary DNA copies of the viral genome by transfection of susceptible cells. These reverse genetics systems have provided investigators with novel and powerful approaches to answer important questions about the biology of influenza viruses, including the function of viral proteins, their interaction with cellular host factors and the mechanisms of influenza virus transmission and pathogenesis. In addition, reverse genetics techniques have allowed the generation of recombinant influenza viruses, providing a powerful technology to develop both inactivated and live-attenuated influenza vaccines. In this review, we will summarize the current knowledge of state-of-the-art, plasmid-based, influenza reverse genetics approaches and their implementation to provide rapid, convenient, safe and more effective influenza inactivated or live-attenuated vaccines.</p></abstract><kwd-group><kwd>influenza vaccines</kwd><kwd>influenza virus</kwd><kwd>reverse genetics</kwd><kwd>live-attenuated influenza virus</kwd><kwd>influenza inactivated virus</kwd><kwd>recombinant influenza virus</kwd><kwd>vaccination</kwd><kwd>antivirals</kwd><kwd>universal vaccines</kwd></kwd-group></article-meta></front><body><sec id="sec1-ijms-18-00020"><title>1. Influenza Virus</title><sec id="sec1dot1-ijms-18-00020"><title>1.1. Influenza Virus Structure and Genome Organization</title><p>Influenza A (IAV) and B (IBV) viruses belong to the <italic>Orthomyxoviridae</italic> family of enveloped viruses [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>]. IAV is able to infect several species and mostly exists in the wild aquatic fowl reservoir [<xref rid="B2-ijms-18-00020" ref-type="bibr">2</xref>,<xref rid="B3-ijms-18-00020" ref-type="bibr">3</xref>,<xref rid="B4-ijms-18-00020" ref-type="bibr">4</xref>]. On the other hand, IBV is mainly restricted and adapted to humans, although sporadic infections of seals have been documented [<xref rid="B5-ijms-18-00020" ref-type="bibr">5</xref>,<xref rid="B6-ijms-18-00020" ref-type="bibr">6</xref>].</p><p>IAV and IBV genomes contain eight negative sense, single-stranded viral (v)RNA segments [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f001">Figure 1</xref>). IAV and IBV vRNAs contain a central coding region that is flanked at both terminal ends by non-coding regions (NCRs), which serve as promoters to initiate genome replication and gene transcription by the viral polymerase complex [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>,<xref rid="B7-ijms-18-00020" ref-type="bibr">7</xref>]. Influenza vRNAs in the virion are found as viral ribonucleoprotein (vRNP) complexes encapsidated by the viral nucleoprotein (NP) and a single copy of the viral polymerase complex. Influenza virus-encoded RNA-dependent RNA polymerase (RdRp) [<xref rid="B8-ijms-18-00020" ref-type="bibr">8</xref>] is a trimeric complex consisting of the polymerase basic 1 (PB1) and 2 (PB2) and acidic (PA) proteins and, together with the viral NP, are the minimal components involved in viral replication and transcription [<xref rid="B9-ijms-18-00020" ref-type="bibr">9</xref>].</p><p>IAV and IBV share many features, but they differ in their host range, virion structure, genomic organization and glycan binding specificities [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>,<xref rid="B10-ijms-18-00020" ref-type="bibr">10</xref>]. Despite having similar genomes encoding homologous proteins, IAV and IBV are distinguished by the different lengths of proteins and non-coding regions (NCRs) that serve as promoters for genome replication and gene transcription [<xref rid="B5-ijms-18-00020" ref-type="bibr">5</xref>,<xref rid="B11-ijms-18-00020" ref-type="bibr">11</xref>,<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f001">Figure 1</xref>). Likewise, they can also be distinguished by the presence of accessory proteins encoded from overlapping open reading frames (ORFs) and by the antigenic differences of internal proteins [<xref rid="B13-ijms-18-00020" ref-type="bibr">13</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f001">Figure 1</xref>A,B). For instance, IAV and IBV both encode ion channel proteins from the gene M segment 7, M2 and BM2, respectively. The M2 and BM2 proteins of IAV or IBV are encoded together with the matrix protein 1 (M1) and both are incorporated into virions and expressed on the surface of virus-infected cells [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>]. However, the M2 protein of IAV is translated from a spliced mRNA [<xref rid="B14-ijms-18-00020" ref-type="bibr">14</xref>], while the IBV BM2 protein is translated using a different strategy, where the initiation codon of BM2 protein overlaps the termination codon of M1 protein (UAAUG, a stop-start pentanucleotide) [<xref rid="B15-ijms-18-00020" ref-type="bibr">15</xref>]. In addition, IBV expresses the NB ion channel, which is absent in type A influenza virus [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f001">Figure 1</xref>B). However, both influenza viruses encode two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA) (<xref ref-type="fig" rid="ijms-18-00020-f001">Figure 1</xref>A,B). IAV and IBV HA proteins are involved in binding to cellular receptors and responsible for the fusion of the viral and endosomal membranes [<xref rid="B16-ijms-18-00020" ref-type="bibr">16</xref>]. Infection with IAV or IBV induces a protective immunity mediated, at least partially, by antibodies directed against the viral HA, which is the main immunogenic target in both natural infections and vaccine approaches. Influenza NA glycoprotein is responsible for the cleavage of sialic acid moieties from sialyloligosaccharides and facilitates the release of newly produced virions from infected cells [<xref rid="B17-ijms-18-00020" ref-type="bibr">17</xref>,<xref rid="B18-ijms-18-00020" ref-type="bibr">18</xref>]. IAVs are classified on the basis of the antigenic properties of HA and NA into 18 HA (H1–H18) and 11 NA (N1–N11) subtypes [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>,<xref rid="B19-ijms-18-00020" ref-type="bibr">19</xref>,<xref rid="B20-ijms-18-00020" ref-type="bibr">20</xref>]. However, only IAV H1N1 and H3N2 subtypes are currently circulating in humans. On the other hand, two major lineages of IBV are circulating in humans, the Victoria-like and Yamagata-like subtypes that are divergent from the ancestral IBV (B/Lee/1940) and have been co-circulating in humans since the 1980s [<xref rid="B5-ijms-18-00020" ref-type="bibr">5</xref>,<xref rid="B21-ijms-18-00020" ref-type="bibr">21</xref>,<xref rid="B22-ijms-18-00020" ref-type="bibr">22</xref>]. These two subtypes are the predominant circulating virus strains about once every three years [<xref rid="B23-ijms-18-00020" ref-type="bibr">23</xref>,<xref rid="B24-ijms-18-00020" ref-type="bibr">24</xref>,<xref rid="B25-ijms-18-00020" ref-type="bibr">25</xref>].</p></sec><sec id="sec1dot2-ijms-18-00020"><title>1.2. Influenza Life Cycle</title><p>Infection with influenza viruses begins when the viral HA protein binds to its cellular receptor, a sialylated glycoprotein containing α-2,3 or α-2,6 linkages [<xref rid="B16-ijms-18-00020" ref-type="bibr">16</xref>]. Upon the binding to the receptor, the uptake of the virus by receptor-mediated endocytosis is initiated, and the cell membrane engulfs the virus particles in an endosome. After endocytosis and upon acidification of the endosome, influenza viral HA undergoes a conformational change responsible for the fusion of the viral and the endosome membrane [<xref rid="B16-ijms-18-00020" ref-type="bibr">16</xref>]. Then, the M2 (IAV) or BM2 (IBV) ion channel proteins promote the release of the vRNP complexes from the virion core into the cytoplasm of infected cells [<xref rid="B14-ijms-18-00020" ref-type="bibr">14</xref>,<xref rid="B26-ijms-18-00020" ref-type="bibr">26</xref>]. The vRNPs are translocated from the cytoplasm to the nucleus of infected cells to initiate viral genome replication and gene transcription [<xref rid="B27-ijms-18-00020" ref-type="bibr">27</xref>]. The nuclear export (NEP) and matrix 1 (M1) proteins are responsible for the nuclear export of newly-synthesized vRNPs into the cytoplasm of infected cells. Notably, a single copy of each of the eight vRNAs is packaged into new virions [<xref rid="B28-ijms-18-00020" ref-type="bibr">28</xref>,<xref rid="B29-ijms-18-00020" ref-type="bibr">29</xref>]. Selective package of influenza vRNAs into nascent virions is mediated by RNA-RNA interactions of vRNA packaging signals present at the terminal ends of each of the vRNA segments [<xref rid="B30-ijms-18-00020" ref-type="bibr">30</xref>]. Finally, the receptor-destroying enzymatic activity of NA is responsible for the release of newly-synthesized viral particles from the surface of infected cells [<xref rid="B17-ijms-18-00020" ref-type="bibr">17</xref>].</p><p>Among the first battles between the host and the virus, cellular type I interferon (IFN-I) plays an important role in controlling viral infection [<xref rid="B31-ijms-18-00020" ref-type="bibr">31</xref>]. Therefore, viruses have developed multiple strategies to hijack the host cellular immune response. Influenza vRNA segment 8, or the nonstructural (NS) gene, encodes two distinct proteins through a direct or alternative splicing mechanism [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>]. Influenza virus segment 8 produces the nonstructural protein 1 (NS1) as a primary transcript, whereas NEP is produced by alternative splicing of the NS mRNA [<xref rid="B32-ijms-18-00020" ref-type="bibr">32</xref>]. NS1 has multiple functions during the replication cycle of influenza virus, but most notably inhibits induction of IFN-I response and innate immune activation [<xref rid="B31-ijms-18-00020" ref-type="bibr">31</xref>].</p></sec><sec id="sec1dot3-ijms-18-00020"><title>1.3. Influenza Viruses and Their Impact on Human Health</title><p>Influenza viruses pose a threat to human health and are responsible for global epidemics every year [<xref rid="B33-ijms-18-00020" ref-type="bibr">33</xref>,<xref rid="B34-ijms-18-00020" ref-type="bibr">34</xref>,<xref rid="B35-ijms-18-00020" ref-type="bibr">35</xref>,<xref rid="B36-ijms-18-00020" ref-type="bibr">36</xref>,<xref rid="B37-ijms-18-00020" ref-type="bibr">37</xref>]. In fact, influenza virus is one of the most significant causes of morbidity and mortality yearly, leading to a significant economic impact [<xref rid="B38-ijms-18-00020" ref-type="bibr">38</xref>]. Despite the implementation of effective and comprehensive vaccination programs, the World Health Organization (WHO) estimates that seasonal influenza virus infections results in about one billion infections, 3–5 million cases of severe disease and between 300,000 and 500,000 deaths around the world annually [<xref rid="B39-ijms-18-00020" ref-type="bibr">39</xref>]. Moreover, just in the United States (U.S.), influenza viral infections result in an average of 87 billion dollars of cost due to prophylactic, therapeutic and hospitalization costs, as well as missed school or work days [<xref rid="B38-ijms-18-00020" ref-type="bibr">38</xref>,<xref rid="B40-ijms-18-00020" ref-type="bibr">40</xref>,<xref rid="B41-ijms-18-00020" ref-type="bibr">41</xref>,<xref rid="B42-ijms-18-00020" ref-type="bibr">42</xref>]. In addition to seasonal influenza, IAV can cause sporadic pandemics of great consequences when novel viruses are introduced into humans [<xref rid="B43-ijms-18-00020" ref-type="bibr">43</xref>]. The mechanisms responsible for the emergence of seasonal and pandemic IAVs are antigenic drift and antigenic shift, respectively [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>,<xref rid="B44-ijms-18-00020" ref-type="bibr">44</xref>,<xref rid="B45-ijms-18-00020" ref-type="bibr">45</xref>,<xref rid="B46-ijms-18-00020" ref-type="bibr">46</xref>]. In the case of seasonal influenza, mutations in the viral genome result in the selection of antigenic variants with changes in viral tropism, increased levels of viral fitness or in the ability to escape neutralizing antibody (NAb) responses induced upon previous natural infections or vaccinations [<xref rid="B44-ijms-18-00020" ref-type="bibr">44</xref>,<xref rid="B45-ijms-18-00020" ref-type="bibr">45</xref>]. Moreover, antigenic drift can lead to variant viruses resistant to antivirals [<xref rid="B47-ijms-18-00020" ref-type="bibr">47</xref>]. On the contrary, antigenic shift results from co-infection of a host cell or organism with two or more IAVs where vRNAs in viral progenies are reassorted [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>,<xref rid="B46-ijms-18-00020" ref-type="bibr">46</xref>]. By obtaining viral segment constellations that confer influenza virus optimal replication, transmissibility and immunologic escape, reassortant IAVs can lead to a pandemic in immunologically-naive populations. IAVs and IBVs can reassort intratypically between subtypes or lineages, but intertypic reassortment, or genetic swapping of segments between IAV and IBV, has not been reported [<xref rid="B21-ijms-18-00020" ref-type="bibr">21</xref>,<xref rid="B48-ijms-18-00020" ref-type="bibr">48</xref>,<xref rid="B49-ijms-18-00020" ref-type="bibr">49</xref>]. The absence of intertypic reassortment is mediated by the lack of compatible packaging signals between IAV and IBV [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>]. In the last century, three IAV pandemics have occurred: the H1N1 Spanish flu of 1918, the H2N2 Asian flu of 1957 and the H3N2 Hong Kong flu of 1968 [<xref rid="B44-ijms-18-00020" ref-type="bibr">44</xref>,<xref rid="B50-ijms-18-00020" ref-type="bibr">50</xref>]. Of these three, the 1918 H1N1 Spanish flu was particularly fatal and responsible for approximately 50 million deaths around the world [<xref rid="B51-ijms-18-00020" ref-type="bibr">51</xref>]. Although not classified as true pandemics, three epidemics of influenza H1N1 viruses in 1947, 1976 and 1977 were feared to have pandemic potential. In 2009, a swine-origin H1N1 IAV was responsible for the first influenza pandemic of the 21st century and infected, in less than one year, more than 600,000 individuals around the world [<xref rid="B52-ijms-18-00020" ref-type="bibr">52</xref>,<xref rid="B53-ijms-18-00020" ref-type="bibr">53</xref>]. Although influenza virus infections are primarily spread by person-to-person transmission via aerosolized droplets, infection with new avian- or swine-origin IAV can take place and may have great risk for pandemic potential if the virus acquires the ability to be transmitted between humans [<xref rid="B54-ijms-18-00020" ref-type="bibr">54</xref>,<xref rid="B55-ijms-18-00020" ref-type="bibr">55</xref>]. IBVs usually contribute less to seasonal epidemics than IAVs of the H3N2 subtype, but they contribute more than type A H1N1 influenza strains and are the predominant circulating virus strains once every three years [<xref rid="B23-ijms-18-00020" ref-type="bibr">23</xref>,<xref rid="B24-ijms-18-00020" ref-type="bibr">24</xref>,<xref rid="B25-ijms-18-00020" ref-type="bibr">25</xref>]. Moreover, during the last decade, IBV has been the cause of several acute respiratory illness outbreaks [<xref rid="B56-ijms-18-00020" ref-type="bibr">56</xref>,<xref rid="B57-ijms-18-00020" ref-type="bibr">57</xref>,<xref rid="B58-ijms-18-00020" ref-type="bibr">58</xref>,<xref rid="B59-ijms-18-00020" ref-type="bibr">59</xref>,<xref rid="B60-ijms-18-00020" ref-type="bibr">60</xref>]. To date, no influenza pandemics have been linked to IBVs.</p></sec><sec id="sec1dot4-ijms-18-00020"><title>1.4. Current Strategies to Combat Human Influenza Infections</title><p>Public health concerns posed by influenza virus infections are aggravated by the ability of influenza viruses to efficiently transmit and the limited therapeutic options to treat viral infections [<xref rid="B37-ijms-18-00020" ref-type="bibr">37</xref>]. Thus, vaccination remains our best medical intervention to protect humans against seasonal influenza virus. However, the efficiency of current influenza vaccines is suboptimal [<xref rid="B61-ijms-18-00020" ref-type="bibr">61</xref>]. The segmented genome of influenza viruses provides an evolutionary advantage of reassortment, or the exchange of viral genome segments between different viral strains within the same type. In addition to this exchange of genome material (or antigenic shift), influenza viruses can introduce mutations in their genomes (or antigenic drift), leading to viral mutants with resistance against current antivirals or NAbs [<xref rid="B62-ijms-18-00020" ref-type="bibr">62</xref>,<xref rid="B63-ijms-18-00020" ref-type="bibr">63</xref>,<xref rid="B64-ijms-18-00020" ref-type="bibr">64</xref>].</p><sec id="sec1dot4dot1-ijms-18-00020"><title>1.4.1. Influenza Vaccines</title><p>Because of the antigenic drift, influenza vaccines need to be reformulated yearly to ensure that the HA and NA present in the vaccine match those present in circulating seasonal viruses. To date, three types of influenza virus vaccines are approved by the Food and Drug Administration (FDA) for human use: recombinant viral HA, inactivated virus and live-attenuated viruses [<xref rid="B65-ijms-18-00020" ref-type="bibr">65</xref>,<xref rid="B66-ijms-18-00020" ref-type="bibr">66</xref>,<xref rid="B67-ijms-18-00020" ref-type="bibr">67</xref>,<xref rid="B68-ijms-18-00020" ref-type="bibr">68</xref>,<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>]. Regardless of the type of vaccine, seasonal influenza vaccines contain antigens from the three circulating influenza virus strains: IAV subtypes H1N1 and H3N2; and IBV (Victoria-like or Yamagata-like lineage) [<xref rid="B67-ijms-18-00020" ref-type="bibr">67</xref>,<xref rid="B70-ijms-18-00020" ref-type="bibr">70</xref>]. Recently, to improve the efficacy of seasonal influenza vaccines, a quadrivalent influenza vaccine formulation that includes both IBV lineages components (Victoria-like and Yamagata-like lineage) has been approved by the FDA [<xref rid="B14-ijms-18-00020" ref-type="bibr">14</xref>].</p><p>The most common influenza vaccine is the inactivated influenza vaccine (IIV). IIV, which is administered intramuscularly, has been shown to elicit protective humoral immunity by producing NAbs that target epitopes on HA [<xref rid="B61-ijms-18-00020" ref-type="bibr">61</xref>,<xref rid="B71-ijms-18-00020" ref-type="bibr">71</xref>]. In contrast to IIV, the live-attenuated influenza vaccine (LAIV) and its administration mimic the natural route of virus infection, which consequently having risks and benefits [<xref rid="B72-ijms-18-00020" ref-type="bibr">72</xref>]. An advantage is that LAIV elicits both more rapid and efficient innate and adaptive immune responses [<xref rid="B65-ijms-18-00020" ref-type="bibr">65</xref>] and can provide more efficient cross-reactive T-cell-mediated protection against heterologous influenza viruses [<xref rid="B72-ijms-18-00020" ref-type="bibr">72</xref>,<xref rid="B73-ijms-18-00020" ref-type="bibr">73</xref>]. Although both IIV and LAIV have been shown to be efficient for the treatment of influenza viral infections, there is an unmet need to increase the effectiveness of seasonal influenza vaccines. Likewise, there is an urgent need to develop effective vaccines for the treatment of potential pandemic influenza viruses.</p></sec><sec id="sec1dot4dot2-ijms-18-00020"><title>1.4.2. Influenza Antivirals</title><p>Although vaccination is the main method to prevent influenza infections in humans, antivirals offer an additional countermeasure against new rapidly-spreading and/or potentially pandemic influenza viruses [<xref rid="B74-ijms-18-00020" ref-type="bibr">74</xref>,<xref rid="B75-ijms-18-00020" ref-type="bibr">75</xref>,<xref rid="B76-ijms-18-00020" ref-type="bibr">76</xref>]. Therapeutic choices to control influenza infection are currently limited to two classes of FDA-approved antivirals targeting either the viral M2 ion channel (amantadine, rimantadine) [<xref rid="B77-ijms-18-00020" ref-type="bibr">77</xref>,<xref rid="B78-ijms-18-00020" ref-type="bibr">78</xref>,<xref rid="B79-ijms-18-00020" ref-type="bibr">79</xref>] or the sialidase active site of NA (oseltamivir, zanamivir) [<xref rid="B80-ijms-18-00020" ref-type="bibr">80</xref>]. The first inhibits the initial steps of the virus life cycle, while the second inhibits virus release. However, NA inhibitors are the only type of antivirals approved for the prophylaxis and treatment of IBV infections [<xref rid="B81-ijms-18-00020" ref-type="bibr">81</xref>], although data from clinical trials have shown that oseltamivir is less effective against IBVs than against IAVs [<xref rid="B82-ijms-18-00020" ref-type="bibr">82</xref>]. Importantly, mutations in the viral genome can lead to influenza antivirals being ineffective, like in the case of M2 blockers, which are no longer recommended against circulating seasonal influenza viral strains [<xref rid="B83-ijms-18-00020" ref-type="bibr">83</xref>]. Therefore, the emergence of drug-resistant influenza variants is an increasing concern for controlling influenza infections [<xref rid="B76-ijms-18-00020" ref-type="bibr">76</xref>,<xref rid="B84-ijms-18-00020" ref-type="bibr">84</xref>], and there is a significant need for the identification of novel compounds with antivirals properties.</p></sec></sec></sec><sec id="sec2-ijms-18-00020"><title>2. Influenza Vaccine Production</title><sec id="sec2dot1-ijms-18-00020"><title>2.1. Vaccine Strain Selection</title><p>Influenza viruses evade human pre-existing immunity by accumulating mutations (antigenic drift), allowing reinfection of individuals previously exposed to natural infections or vaccinated. Thus, vaccine companies have to reformulate the composition of influenza vaccines yearly to ensure a good match between the viruses present in the vaccine and those seasonally circulating in humans [<xref rid="B33-ijms-18-00020" ref-type="bibr">33</xref>,<xref rid="B34-ijms-18-00020" ref-type="bibr">34</xref>,<xref rid="B85-ijms-18-00020" ref-type="bibr">85</xref>]. The WHO Global Influenza Surveillance Network (GISN) [<xref rid="B35-ijms-18-00020" ref-type="bibr">35</xref>], which includes more than 120 National Influenza Centers in over 90 countries [<xref rid="B86-ijms-18-00020" ref-type="bibr">86</xref>], tracks the evolution and epidemiology of influenza viruses and uses the collected data for the vaccine strain selection process. These studies also help to understand virus evolution and epidemiology in different geographic areas [<xref rid="B19-ijms-18-00020" ref-type="bibr">19</xref>]. Each center collects and analyzes samples of seasonal influenza viruses for communication to the WHO Collaborating Centers for Reference and Research on Influenza: (1) The Centers for Disease Control and Prevention (CDC) in Atlanta, USA; (2) The National Institute for Medical Research in Mill Hill, U.K.; (3) The WHO Collaborating Centre for Reference and Research on Influenza in Melbourne, Australia; and (4) The National Institute of Infectious Disease in Tokyo, Japan. Basically, the degree of immunity induced by one influenza strain that is effective against another strain is mainly dependent on the antigenic difference between both viral strains [<xref rid="B87-ijms-18-00020" ref-type="bibr">87</xref>,<xref rid="B88-ijms-18-00020" ref-type="bibr">88</xref>]. The influenza viral glycoprotein HA, the primary target of the protective neutralizing immune responses [<xref rid="B88-ijms-18-00020" ref-type="bibr">88</xref>,<xref rid="B89-ijms-18-00020" ref-type="bibr">89</xref>], is the focus of influenza virus surveillance and the primary component targeted by currently licensed influenza vaccines [<xref rid="B72-ijms-18-00020" ref-type="bibr">72</xref>,<xref rid="B89-ijms-18-00020" ref-type="bibr">89</xref>].</p><p>Influenza vaccine production is challenging because of the wait time that is needed to identify the predominant circulating virus and the production time that is needed to manufacture the vaccine. The National Influenza Centers perform virus isolation on certain samples obtained from patients to identify circulating viruses and to determine if the viruses grow efficiently in culture. Normally, influenza viruses are isolated using Madin Darby canine kidney (MDCK) cells instead of chicken embryonated eggs because higher isolation rates, especially for H3N2 IAV strains, have been reported using MDCK cells [<xref rid="B90-ijms-18-00020" ref-type="bibr">90</xref>,<xref rid="B91-ijms-18-00020" ref-type="bibr">91</xref>,<xref rid="B92-ijms-18-00020" ref-type="bibr">92</xref>]. Although most influenza viruses are isolated from mammalian cells, viruses must be generated in embryonated eggs for vaccine manufacturing due to regulations [<xref rid="B93-ijms-18-00020" ref-type="bibr">93</xref>]. Nowadays, the vaccine strains must be selected almost 7–9 months ahead of the influenza season in which they will be used. The recommendation for the strains included in the vaccine composition for the Northern Hemisphere is made in February to allow time for the ~300 million doses of vaccine to be produced in time for vaccinating people in October/November. This allows for influenza season preparation, which typically peaks sometime between December and March [<xref rid="B86-ijms-18-00020" ref-type="bibr">86</xref>]. On the other hand, for the Southern Hemisphere, recommendations are provided in September, and vaccination takes place in March/April of the following year [<xref rid="B86-ijms-18-00020" ref-type="bibr">86</xref>].</p></sec><sec id="sec2dot2-ijms-18-00020"><title>2.2. Types of Influenza Seasonal Vaccines</title><p>Seasonal influenza vaccines must protect against H1N1, H3N2 and B viral strains currently circulating in humans globally [<xref rid="B34-ijms-18-00020" ref-type="bibr">34</xref>,<xref rid="B86-ijms-18-00020" ref-type="bibr">86</xref>]. The main goals of influenza vaccines are the protection against infection and disease caused by influenza infections and to restrict virus transmission within the population [<xref rid="B67-ijms-18-00020" ref-type="bibr">67</xref>,<xref rid="B94-ijms-18-00020" ref-type="bibr">94</xref>]. NAb responses, commonly assessed by measuring hemagglutination inhibition (HAI) titers, are currently used as a serological marker of the efficient immunological response to the vaccine. The effectiveness of influenza vaccines is variable and usually higher in children and in healthy adults under the age of 65. In individuals above 65 years of age, lower effectiveness has been observed [<xref rid="B61-ijms-18-00020" ref-type="bibr">61</xref>,<xref rid="B71-ijms-18-00020" ref-type="bibr">71</xref>,<xref rid="B95-ijms-18-00020" ref-type="bibr">95</xref>]. Recommendations for influenza vaccination differ between countries, but since influenza vaccines do not induce long-lasting antibody protection, annual influenza vaccinations are recommended. Nowadays, the most used influenza vaccines can be divided into IIV and LAIV [<xref rid="B65-ijms-18-00020" ref-type="bibr">65</xref>,<xref rid="B66-ijms-18-00020" ref-type="bibr">66</xref>,<xref rid="B72-ijms-18-00020" ref-type="bibr">72</xref>,<xref rid="B73-ijms-18-00020" ref-type="bibr">73</xref>,<xref rid="B96-ijms-18-00020" ref-type="bibr">96</xref>].</p><sec id="sec2dot2dot1-ijms-18-00020"><title>2.2.1. Influenza Inactivated Vaccine (IIV)</title><p>Killed virus vaccines or IIV are generally administered intramuscularly and can be classified as whole virus vaccines or split vaccines [<xref rid="B66-ijms-18-00020" ref-type="bibr">66</xref>,<xref rid="B71-ijms-18-00020" ref-type="bibr">71</xref>,<xref rid="B97-ijms-18-00020" ref-type="bibr">97</xref>,<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>]. Whole virus vaccines were the first to be developed. The influenza virus is grown in embryonated chicken eggs, subsequently purified, concentrated and chemically inactivated with formaldehyde [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>). Whole virus vaccines are safe and well tolerated, with an efficacy of 60%–90% in children and adults. On the other hand, the split-virus vaccine exposes all viral proteins and subviral elements upon dissociation of the virions by a nonionic detergent treatment step [<xref rid="B97-ijms-18-00020" ref-type="bibr">97</xref>,<xref rid="B99-ijms-18-00020" ref-type="bibr">99</xref>]. Most influenza vaccines in the U.S. and Europe are egg-produced, formaldehyde-inactivated, then chemically disrupted with nonionic detergents after purification.</p><p>Unlike virus-based vaccines, subunit influenza vaccines consist of purified viral HA or HA/NA proteins without the other viral components [<xref rid="B68-ijms-18-00020" ref-type="bibr">68</xref>]. These subunit vaccines can be produced in eggs if the viral proteins are prepared from viruses where HA/NA have been purified by removal of other viral component. In addition, the subunit vaccines can be generated using novel manufacturing technologies [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>,<xref rid="B100-ijms-18-00020" ref-type="bibr">100</xref>], which allows for the production of large quantities of the viral HA using baculovirus expression systems and recombinant DNA technologies [<xref rid="B100-ijms-18-00020" ref-type="bibr">100</xref>].</p><p>The IAV strains that are used in vaccine manufacture are high-growth 6 + 2 reassortants containing the HA and NA gene segments from the target strains in the backbone of influenza A/Puerto Rico/8/1934 H1N1 (PR8) or other high growth virus. Influenza PR8 replicates at high titers in eggs and cells and also has a favorable safety profile in humans [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>,<xref rid="B101-ijms-18-00020" ref-type="bibr">101</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>A). To generate the reassortant viruses, eggs are co-infected with PR8 and seasonal strains (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>A). Selection of appropriated seed vaccine viruses is made by amplification in the presence of PR8 HA and NA NAbs [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>A). Then, selected viruses are cloned and sequenced for confirmation. The IBV vaccines are typically wild-type (WT) viruses. However, IBV reassortants are used if WT IBV growth properties are not optimal for efficient growth and vaccine production [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>].</p></sec><sec id="sec2dot2dot2-ijms-18-00020"><title>2.2.2. Live-Attenuated Influenza Vaccine</title><p>The remaining class of vaccines consists of live-attenuated influenza viruses. Attenuated human LAIVs were developed in the 1960s by serial passage of the virus in eggs using suboptimal conditions of temperature. The resulting attenuated viruses displayed a temperature-sensitive (ts) cold-adapted (ca) attenuated (att) phenotype that grew at 25 °C, but not at temperatures found in the lower respiratory tract (>35 °C) [<xref rid="B102-ijms-18-00020" ref-type="bibr">102</xref>,<xref rid="B103-ijms-18-00020" ref-type="bibr">103</xref>,<xref rid="B104-ijms-18-00020" ref-type="bibr">104</xref>,<xref rid="B105-ijms-18-00020" ref-type="bibr">105</xref>]. Because this ts, ca, att phenotype restricts virus replication to the upper respiratory track, these viruses could induce local protective immunological responses [<xref rid="B65-ijms-18-00020" ref-type="bibr">65</xref>,<xref rid="B95-ijms-18-00020" ref-type="bibr">95</xref>,<xref rid="B96-ijms-18-00020" ref-type="bibr">96</xref>,<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>].</p><p>LAIV have been available in the U.S. since 2003 and are administrated intranasally. The advantages of a live virus vaccine, as compared to the inactivated virus vaccine, is that it is applied to the nasal mucosa where the vaccine can induce local immunity (including NAbs), generate a cell-mediated immune response and provide a cross-reactive and longer lasting immune response [<xref rid="B106-ijms-18-00020" ref-type="bibr">106</xref>]. The current LAIVs consist of the internal viral segments (PB2, PB1, PA, NP, M and NS) of an attenuated master donor virus (MDV) and the HA and NA viral segments from the selected seasonal virus strain (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>B). The MDVs used are A/Ann Arbor/6/60 (H2N2) and B/Ann Arbor/1/66 for IAV and IBV, respectively [<xref rid="B103-ijms-18-00020" ref-type="bibr">103</xref>,<xref rid="B104-ijms-18-00020" ref-type="bibr">104</xref>,<xref rid="B107-ijms-18-00020" ref-type="bibr">107</xref>,<xref rid="B108-ijms-18-00020" ref-type="bibr">108</xref>,<xref rid="B109-ijms-18-00020" ref-type="bibr">109</xref>,<xref rid="B110-ijms-18-00020" ref-type="bibr">110</xref>,<xref rid="B111-ijms-18-00020" ref-type="bibr">111</xref>,<xref rid="B112-ijms-18-00020" ref-type="bibr">112</xref>,<xref rid="B113-ijms-18-00020" ref-type="bibr">113</xref>]. Similar to MDV IAV, MDV IBV was originally derived by serial passage of the parental WT virus and isolated at successively reduced temperatures in primary chicken kidney (PCK) cells [<xref rid="B114-ijms-18-00020" ref-type="bibr">114</xref>]. The resulting IBV MDV grows efficiently at 25 °C (ca), but its growth is restricted at 37 °C (ts). The genetic changes in the MDV strains have been recently characterized. The MDV IAV includes five mutations in two of the viral polymerases (PB2 N265S; and PB1 K391E, D581G and A661T) and NP (D34G) [<xref rid="B103-ijms-18-00020" ref-type="bibr">103</xref>,<xref rid="B104-ijms-18-00020" ref-type="bibr">104</xref>,<xref rid="B115-ijms-18-00020" ref-type="bibr">115</xref>]. The MDV IBV has been reported to contain two mutant amino acids in NP (A114 and H410) and one in PA (M431) that are responsible for the ts, ca signature [<xref rid="B108-ijms-18-00020" ref-type="bibr">108</xref>]. Two additional residues in M1 (Q159 and V183) provide the MDV IBV an attenuated (att) phenotype [<xref rid="B108-ijms-18-00020" ref-type="bibr">108</xref>]. Although LAIVs have been approved for clinical use, to date, their mechanism of attenuation has not been completely understood. However, the tolerability of LAIVs in specific populations is an important concern because of the inherent risk of immunizing with live viruses. Thus, LAIVs are not recommended for immunocompromised patients or asthmatics [<xref rid="B116-ijms-18-00020" ref-type="bibr">116</xref>] and are not approved for use in children under two years of age [<xref rid="B72-ijms-18-00020" ref-type="bibr">72</xref>,<xref rid="B117-ijms-18-00020" ref-type="bibr">117</xref>]. Moreover, LAIVs harboring different HA and NA viral segments can be unequally safe or immunogenic from year to year as the viral HA and NA are different. Similar to U.S. LAIVs, in Russia, two ts, ca MDV IAVs have been obtained by using a similar temperature adaptation approach. The two Russian MDVs were originated from the same parental A/Leningrad/134/57 H2N2 (Len/57) influenza strain [<xref rid="B118-ijms-18-00020" ref-type="bibr">118</xref>,<xref rid="B119-ijms-18-00020" ref-type="bibr">119</xref>]. Similarly to the U.S. MDV, the Russian MDVs have been selected by growing Len/57 in embryonated chicken eggs at lower (25 °C) temperatures. The MDV Len/17 was obtained after 17 passages of Len/57 at 25 °C and has been used in the preparation of the Russian LAIV for use in adults. The genetic changes in the MDVs Len/17 have been identified, and these include four mutations in three viral proteins (PB2 V478L; PB1 K265N and V591I; NEP M100I) [<xref rid="B120-ijms-18-00020" ref-type="bibr">120</xref>]. The second donor strain (Len/47) was obtained after a total of 47 passages and has been used for vaccinating children less than 16 years of age, although the genetic changes responsible for the further ts, ca phenotype have not been well characterized [<xref rid="B121-ijms-18-00020" ref-type="bibr">121</xref>].</p><p>In all cases, like for IIV, the MDVs contain six internal genes to generate vaccine strains in combination with the seasonal recommended HA and NA genes from the circulating strains (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>B). LAIVs are generated either by classical reassortment in eggs (as previously described for the IIV) (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>B) or by reverse genetics, as indicated below [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>].</p></sec></sec><sec id="sec2dot3-ijms-18-00020"><title>2.3. Substrates for the Production of Influenza Vaccines</title><p>Most of the influenza virus vaccines have been traditionally produced in eggs, but with the progress made in mammalian cell culture technologies, influenza vaccine manufacturers have invested in these novel cell culture systems for the mass production of influenza vaccines without the need of eggs.</p><sec id="sec2dot3dot1-ijms-18-00020"><title>2.3.1. Chicken Embryonated Eggs</title><p>The majority of the currently licensed influenza vaccines that are made by biotechnology companies use fertilized chicken egg-based production technology, but this process has multiple drawbacks. This form of manufacturing depends on the access to embryonated eggs, relies on the ability of influenza viruses to efficiently grow in eggs and is a resource- and time-intensive process [<xref rid="B91-ijms-18-00020" ref-type="bibr">91</xref>,<xref rid="B122-ijms-18-00020" ref-type="bibr">122</xref>,<xref rid="B123-ijms-18-00020" ref-type="bibr">123</xref>,<xref rid="B124-ijms-18-00020" ref-type="bibr">124</xref>]. Moreover, the risk of egg contamination by avian pathogens or microbes represents a risk for the production of influenza vaccines [<xref rid="B125-ijms-18-00020" ref-type="bibr">125</xref>,<xref rid="B126-ijms-18-00020" ref-type="bibr">126</xref>]. Importantly, in the case of an IAV pandemic, the egg supplies can be compromised. For IIV, one dose for adults contains approximately 45 µg of HA (15 µg of viral HA for each of the three antigenic H1N1, H3N2 and IBV components), meaning one egg = 7–10 dose of vaccine. LAIVs share similar egg-based production process steps. The LAIV is recovered from infected eggs and then purified and concentrated [<xref rid="B90-ijms-18-00020" ref-type="bibr">90</xref>]. Importantly, new vaccine production approaches that do not depend on the propagation of influenza viruses in eggs (e.g., cell cultures) represent an excellent option to increase influenza vaccine production.</p></sec><sec id="sec2dot3dot2-ijms-18-00020"><title>2.3.2. Cell Cultures</title><p>Mammalian cell cultures have been used in the biopharmaceutical industry for the production of therapeutic proteins and/or vaccines [<xref rid="B127-ijms-18-00020" ref-type="bibr">127</xref>,<xref rid="B128-ijms-18-00020" ref-type="bibr">128</xref>]. In 2012, the FDA approved a cell-based production process for influenza vaccines, but the manufacturing process begins with egg-grown vaccine viruses per FDA regulations. Influenza vaccine production using FDA-approved MDCK or Vero (African green monkey kidney) cells may eventually and completely replace the use of eggs for the production of influenza vaccines in the future. Influenza vaccine production in mammalian cell lines offers several advantages over egg-based production: it allows faster and greater production capacity, improved availability of substrate for virus growth [<xref rid="B122-ijms-18-00020" ref-type="bibr">122</xref>,<xref rid="B127-ijms-18-00020" ref-type="bibr">127</xref>] and eliminates reliance on the supply of embryonated chicken eggs [<xref rid="B129-ijms-18-00020" ref-type="bibr">129</xref>,<xref rid="B130-ijms-18-00020" ref-type="bibr">130</xref>]. In addition, cell cultures can be cryopreserved and scaled up in bioreactors at any time.</p></sec></sec><sec id="sec2dot4-ijms-18-00020"><title>2.4. Adjuvants</title><p>Adjuvants have been shown to enhance the immune response elicited by an antigen and could be used to improve the immunogenicity of IIV [<xref rid="B131-ijms-18-00020" ref-type="bibr">131</xref>,<xref rid="B132-ijms-18-00020" ref-type="bibr">132</xref>]. The use of adjuvants could also reduce vaccine dose, stretching antigen and vaccine supplies. Currently, FDA-licensed adjuvants for influenza vaccine usage include aluminum salt (alum) and the squalene oil-in-water emulsion systems MF59 (Wadman 2005 (Novartis)) [<xref rid="B133-ijms-18-00020" ref-type="bibr">133</xref>] and AS03 (GlaxoSmithKline) [<xref rid="B134-ijms-18-00020" ref-type="bibr">134</xref>]. However, most of the current IIVs do not contain any type of adjuvant, but many are under investigation.</p></sec></sec><sec id="sec3-ijms-18-00020"><title>3. Influenza Reverse Genetics for Vaccine Development</title><sec id="sec3dot1-ijms-18-00020"><title>3.1. Influenza Reverse Genetics</title><p>Genetics techniques to generate recombinant viruses were first developed for DNA viruses and based on the transfection of cells with plasmids encoding the viral genome or by heterologous recombination between plasmids bearing viral sequences with the virus genome and the helper virus [<xref rid="B135-ijms-18-00020" ref-type="bibr">135</xref>]. Initial genetics approaches for DNA viruses were followed by manipulations of positive-sense RNA viral genomes [<xref rid="B136-ijms-18-00020" ref-type="bibr">136</xref>,<xref rid="B137-ijms-18-00020" ref-type="bibr">137</xref>]. Transfection of plasmid DNA, or RNA transcribed directly in vitro from plasmids, containing the genome of poliovirus into susceptible cells led to the generation of recombinant infectious poliovirus [<xref rid="B138-ijms-18-00020" ref-type="bibr">138</xref>]. However, the genomes of negative-sense RNA viruses, including influenza, were less suitable to molecular biology manipulations in comparison with DNA or positive-sense RNA viruses since their genomes are complementary to mRNA in their orientation and, therefore, not infectious by themselves [<xref rid="B1-ijms-18-00020" ref-type="bibr">1</xref>,<xref rid="B7-ijms-18-00020" ref-type="bibr">7</xref>]. They require the presence of vRNA(s) and the viral RdRps to initiate the replication cycle of the virus [<xref rid="B33-ijms-18-00020" ref-type="bibr">33</xref>,<xref rid="B44-ijms-18-00020" ref-type="bibr">44</xref>]. The advent of reverse genetics and molecular engineering has transformed the influenza field, allowing multiple questions to be answered using genetically-engineered recombinant influenza viruses [<xref rid="B139-ijms-18-00020" ref-type="bibr">139</xref>]. Such studies include mechanisms of viral genome replication and gene transcription, pathogenicity and virulence, virus-host interactions or host range and transmissibility [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>,<xref rid="B13-ijms-18-00020" ref-type="bibr">13</xref>,<xref rid="B139-ijms-18-00020" ref-type="bibr">139</xref>,<xref rid="B140-ijms-18-00020" ref-type="bibr">140</xref>]. Moreover, these technologies have been implemented to develop influenza vaccines [<xref rid="B141-ijms-18-00020" ref-type="bibr">141</xref>] and to generate recombinant influenza viruses expressing foreign proteins as vaccine vectors [<xref rid="B142-ijms-18-00020" ref-type="bibr">142</xref>,<xref rid="B143-ijms-18-00020" ref-type="bibr">143</xref>,<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>] or harboring reporter genes to easily track viral infections [<xref rid="B142-ijms-18-00020" ref-type="bibr">142</xref>,<xref rid="B145-ijms-18-00020" ref-type="bibr">145</xref>,<xref rid="B146-ijms-18-00020" ref-type="bibr">146</xref>,<xref rid="B147-ijms-18-00020" ref-type="bibr">147</xref>].</p><p>Plasmid-based reverse genetics for influenza virus allows for the simultaneous expression of the viral components involved in viral genome replication and gene transcription (PB2, PB1, PA and NP) and the eight negative-stranded vRNAs in transfected susceptible cells, which together generate de novo, recombinant IAVs or IBVs (<xref ref-type="fig" rid="ijms-18-00020-f003">Figure 3</xref>) [<xref rid="B48-ijms-18-00020" ref-type="bibr">48</xref>]. The goal to generate vRNA in vivo from cloned complementary (c)DNAs was achieved when the RNA polymerase I (Pol I) system for influenza vRNA synthesis was established [<xref rid="B148-ijms-18-00020" ref-type="bibr">148</xref>,<xref rid="B149-ijms-18-00020" ref-type="bibr">149</xref>]. Pol I is a nuclear enzyme that transcribes ribosomal (r)RNA, which like influenza vRNA does not contain a cap structure on the 5′ or poly (A) structures on the 3′ ends [<xref rid="B150-ijms-18-00020" ref-type="bibr">150</xref>,<xref rid="B151-ijms-18-00020" ref-type="bibr">151</xref>]. Importantly, Pol I initiates and terminates transcription at defined promoter and terminator sequences allowing the generation of vRNAs without additional nucleotides at their 5′ or 3′ ends, which is required for efficient generation of recombinant viruses using reverse genetics. Nevertheless, the Pol I promoter is species specific [<xref rid="B152-ijms-18-00020" ref-type="bibr">152</xref>,<xref rid="B153-ijms-18-00020" ref-type="bibr">153</xref>] and was originally established for influenza rescue in human cells [<xref rid="B135-ijms-18-00020" ref-type="bibr">135</xref>,<xref rid="B140-ijms-18-00020" ref-type="bibr">140</xref>,<xref rid="B149-ijms-18-00020" ref-type="bibr">149</xref>,<xref rid="B154-ijms-18-00020" ref-type="bibr">154</xref>]. Currently, the Pol I promoters of different species have been identified, allowing the generation of recombinant influenza viruses using reverse genetics techniques in avian, canine, equine or murine cells lines [<xref rid="B155-ijms-18-00020" ref-type="bibr">155</xref>,<xref rid="B156-ijms-18-00020" ref-type="bibr">156</xref>,<xref rid="B157-ijms-18-00020" ref-type="bibr">157</xref>,<xref rid="B158-ijms-18-00020" ref-type="bibr">158</xref>].</p><p>Influenza viruses require the presence of eight vRNA segments for efficient virus fitness and successful production of virion progeny. The initial description of influenza reverse genetics, originally established in 1999 for IAV [<xref rid="B154-ijms-18-00020" ref-type="bibr">154</xref>,<xref rid="B159-ijms-18-00020" ref-type="bibr">159</xref>], required the use of 12 plasmids to generate recombinant influenza viruses: four polymerase II (Pol II) protein expression plasmids, encoding the viral RdRp complex (PB2, PB1 and PA) and NP for vRNP reconstitution; and eight Pol I-driven plasmids for expression of the eight vRNA segments [<xref rid="B154-ijms-18-00020" ref-type="bibr">154</xref>,<xref rid="B159-ijms-18-00020" ref-type="bibr">159</xref>]. However, it was later described that only eight ambisense and/or bidirectional plasmids were needed for complete reconstitution of influenza viruses (<xref ref-type="fig" rid="ijms-18-00020-f003">Figure 3</xref>A) [<xref rid="B150-ijms-18-00020" ref-type="bibr">150</xref>,<xref rid="B151-ijms-18-00020" ref-type="bibr">151</xref>]. The eight plasmid-based rescue system is now the most common method for the generation of recombinant influenza viruses. Because fewer plasmids are required, the eight-plasmid approach is more successful than the initial twelve-plasmid reverse genetic technique. The core of the eight-plasmid rescue system is that each plasmid contains an “ambisense cassette” that includes RNA Pol I and/or II sequences, which drives the transcription of vRNAs (Pol I) and protein (Pol II) expression from the same viral cDNAs (<xref ref-type="fig" rid="ijms-18-00020-f003">Figure 3</xref>B). Using a similar technology in 2002, this reverse genetics technique allowed the recovery of IBV entirely from ambisense plasmids [<xref rid="B13-ijms-18-00020" ref-type="bibr">13</xref>,<xref rid="B160-ijms-18-00020" ref-type="bibr">160</xref>,<xref rid="B161-ijms-18-00020" ref-type="bibr">161</xref>,<xref rid="B162-ijms-18-00020" ref-type="bibr">162</xref>]. Now, IAV and IBV reverse genetics techniques are well established and commonly used in multiple research laboratories for different research purposes.</p></sec><sec id="sec3dot2-ijms-18-00020"><title>3.2. Reverse Genetics for the Investigation of Influenza Virus</title><p>Influenza reverse genetics techniques have had an important effect on expanding our knowledge of the molecular biology and pathogenesis of influenza viruses, allowing researchers to answer important questions in the biology of IAV and IBV that were not possible using conventional virological or biochemical procedures [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>,<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>,<xref rid="B147-ijms-18-00020" ref-type="bibr">147</xref>]. Scientists can now mutate specific nucleotides in the influenza viral genome to elucidate the nature of regulatory sequences or the contribution of specific amino acids to the function of influenza viral proteins. For instance, reverse genetics technologies have enabled the identification and characterization of the <italic>cis</italic>-acting elements required for virus cell entry, uncoating, genome replication and gene transcription, encapsidation, packaging and viral release [<xref rid="B13-ijms-18-00020" ref-type="bibr">13</xref>,<xref rid="B135-ijms-18-00020" ref-type="bibr">135</xref>,<xref rid="B163-ijms-18-00020" ref-type="bibr">163</xref>,<xref rid="B164-ijms-18-00020" ref-type="bibr">164</xref>,<xref rid="B165-ijms-18-00020" ref-type="bibr">165</xref>]. Moreover, by engineering viral vectors suitable for the expression of foreign proteins in infected cells, multiple recombinant influenza viruses harboring reporter fluorescent and/or luminescent genes have been generated and used to identify antivirals or NAbs in vitro and/or in vivo [<xref rid="B142-ijms-18-00020" ref-type="bibr">142</xref>,<xref rid="B146-ijms-18-00020" ref-type="bibr">146</xref>,<xref rid="B147-ijms-18-00020" ref-type="bibr">147</xref>]. Finally, reverse genetics have allowed the creation of single-cycle infectious IAVs (sciIAVs) that are restricted to one cycle of replication in parental cell lines. However, in complementing cell lines, sciIAVs can replicate efficiently and to levels comparable to WT forms of influenza viruses [<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. Highly virulent IAV have the potential to pose a greater human threat than many other Biosafety Level (BSL)-3 and BSL-4 pathogens because of their efficient transmission and limited therapeutic options [<xref rid="B47-ijms-18-00020" ref-type="bibr">47</xref>]. Traditional immunological approaches (e.g., HAI or microneutralization assays) to identify the presence of IAV NAbs rely on the manipulation of live forms of viruses and need the use of special BSL conditions. Thus, novel approaches that allow the detection of viral NAbs without the use of live forms of IAVs and highly contained BSL laboratories would facilitate these serological assays. In this regard and because of their safety profile, sciIAVs represent an excellent alternative to identify new antivirals and/or NAbs [<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. Moreover, several sciIAVs have been shown to represent an excellent option for their implementation as safe, immunogenic and protective vaccines and/or vaccine vectors [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>].</p></sec><sec id="sec3dot3-ijms-18-00020"><title>3.3. Generation of Recombinant Influenza Viruses Using Reverse Genetics Approaches</title><p>Influenza plasmid-based reverse genetics represent a better alternative to circumvent the process of generating reassortant virus by co-infection of chicken embryonated eggs for the generation of influenza vaccines (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>) [<xref rid="B135-ijms-18-00020" ref-type="bibr">135</xref>]. Moreover, the generation of recombinant influenza viruses using plasmid-based reverse genetics approaches is simple, well established and currently in use in several laboratories around the world [<xref rid="B139-ijms-18-00020" ref-type="bibr">139</xref>,<xref rid="B166-ijms-18-00020" ref-type="bibr">166</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f004">Figure 4</xref>). Briefly, the eight-ambisense plasmids are co-transfected into susceptible FDA-approved cells, and viable virus can be recovered from the tissue culture supernatants [<xref rid="B139-ijms-18-00020" ref-type="bibr">139</xref>,<xref rid="B166-ijms-18-00020" ref-type="bibr">166</xref>], then amplified in either embryonic eggs or FDA-approved for vaccine production cells (<xref ref-type="fig" rid="ijms-18-00020-f004">Figure 4</xref>).</p></sec><sec id="sec3dot4-ijms-18-00020"><title>3.4. Influenza Reverse Genetics for the Development of IIV</title><p>For many years, IIVs have been produced by reassortment in eggs (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>) [<xref rid="B90-ijms-18-00020" ref-type="bibr">90</xref>,<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>,<xref rid="B122-ijms-18-00020" ref-type="bibr">122</xref>]. However, strains with the desired genotype (six internal genes of a high-growth virus and the HA and NA glycoproteins of the seasonal influenza virus) could be produced easily and more quickly by implementing reverse genetics approaches. This process would overcome the need of chicken embryonated eggs to generate the desired reassortant virus and, therefore, minimizing the time associated with the selection process of the reassortant IIV (<xref ref-type="fig" rid="ijms-18-00020-f004">Figure 4</xref>A). Moreover, the improvements in surveillance, as well as the de novo gene synthesis for the production of the HA and NA viral segments from the selected strains could reduce considerably the time of vaccine production. Although influenza reverse genetics could be useful for the production of vaccine seed strains, to date, it has not been possible to predict which gene segment(s) constellations are required for efficient growth of different vaccine viruses. Moreover, the time to produce vaccine viruses using reverse genetics versus the time needed to generate them using the classical reassortment approach could be a major consideration for the cost effectiveness in vaccine production and manufacturing. Thus, the traditional viral reassortment has remained preferred because it allows the generation of a number of diverse gene(s) combinations in order to select recombinant viruses with better fitness [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>].</p></sec><sec id="sec3dot5-ijms-18-00020"><title>3.5. Influenza Reverse Genetics Approaches for the Development of Live-Attenuated Influenza Vaccines (LAIVs)</title><p>During the last few decades, considerable improvements have been accomplished in the development of influenza vaccines. However, novel approaches to increase the effectiveness of seasonal influenza vaccines are needed. Reverse genetics technologies have proven a valuable tool to develop reassortant strains for the production of LAIV candidates (<xref ref-type="fig" rid="ijms-18-00020-f004">Figure 4</xref>B). Currently, seed viruses containing six gene segments from the MDV A/Ann Arbor/6/60 (H2N2) and the HA and NA from the selected seasonal virus can be quickly generated using reverse genetics systems [<xref rid="B98-ijms-18-00020" ref-type="bibr">98</xref>,<xref rid="B131-ijms-18-00020" ref-type="bibr">131</xref>] (<xref ref-type="fig" rid="ijms-18-00020-f004">Figure 4</xref>B). The HA and NA from selected seasonal influenza viruses can be amplified by RT-PCR or quickly chemically synthetized and then cloned in ambisense plasmids used for virus rescue. This technology could speed up the development of new LAIVs, bypassing the need to isolate the exact virus reassortment in eggs (<xref ref-type="fig" rid="ijms-18-00020-f002">Figure 2</xref>).</p></sec><sec id="sec3dot6-ijms-18-00020"><title>3.6. Taking Advantage of Reverse Genetics for the Development of Novel Vaccines</title><p>The best way to combat influenza virus infection is to prevent it. Thus, an urgent need exists to develop novel and more effective influenza vaccines. Plasmid-based reverse genetic technologies have allowed the engineering of recombinant influenza viruses that contain single or multiple mutations in the viral genome, which can be potentially implemented as novel or improved vaccine approaches. In fact, several novel vaccine candidates have been developed with promising results in animal models of experimentation. Here, we review and discuss some of them.</p><sec id="sec3dot6dot1-ijms-18-00020"><title>3.6.1. NS1 Truncated or Deficient Viruses as LAIVs</title><p>Because of NS1’s ability to hijack the host innate immune IFN-I response, a variety of potential vaccine strategies have been developed, which are based on the use of modified NS1 proteins as a means for virus attenuation [<xref rid="B167-ijms-18-00020" ref-type="bibr">167</xref>,<xref rid="B168-ijms-18-00020" ref-type="bibr">168</xref>,<xref rid="B169-ijms-18-00020" ref-type="bibr">169</xref>,<xref rid="B170-ijms-18-00020" ref-type="bibr">170</xref>,<xref rid="B171-ijms-18-00020" ref-type="bibr">171</xref>,<xref rid="B172-ijms-18-00020" ref-type="bibr">172</xref>]. Equine [<xref rid="B170-ijms-18-00020" ref-type="bibr">170</xref>], swine [<xref rid="B169-ijms-18-00020" ref-type="bibr">169</xref>,<xref rid="B173-ijms-18-00020" ref-type="bibr">173</xref>,<xref rid="B174-ijms-18-00020" ref-type="bibr">174</xref>], avian [<xref rid="B167-ijms-18-00020" ref-type="bibr">167</xref>,<xref rid="B175-ijms-18-00020" ref-type="bibr">175</xref>,<xref rid="B176-ijms-18-00020" ref-type="bibr">176</xref>], canine [<xref rid="B177-ijms-18-00020" ref-type="bibr">177</xref>] and human [<xref rid="B178-ijms-18-00020" ref-type="bibr">178</xref>,<xref rid="B179-ijms-18-00020" ref-type="bibr">179</xref>] IAVs with partial truncations in or deletions of the viral NS1 protein are all attenuated in vitro and in vivo [<xref rid="B167-ijms-18-00020" ref-type="bibr">167</xref>,<xref rid="B169-ijms-18-00020" ref-type="bibr">169</xref>,<xref rid="B170-ijms-18-00020" ref-type="bibr">170</xref>,<xref rid="B179-ijms-18-00020" ref-type="bibr">179</xref>]. Importantly, these recombinant IAVs can induce a protective immune response upon a single intranasal vaccination in mice [<xref rid="B177-ijms-18-00020" ref-type="bibr">177</xref>,<xref rid="B179-ijms-18-00020" ref-type="bibr">179</xref>,<xref rid="B180-ijms-18-00020" ref-type="bibr">180</xref>,<xref rid="B181-ijms-18-00020" ref-type="bibr">181</xref>], horses [<xref rid="B170-ijms-18-00020" ref-type="bibr">170</xref>], pigs [<xref rid="B169-ijms-18-00020" ref-type="bibr">169</xref>,<xref rid="B173-ijms-18-00020" ref-type="bibr">173</xref>,<xref rid="B174-ijms-18-00020" ref-type="bibr">174</xref>], birds [<xref rid="B167-ijms-18-00020" ref-type="bibr">167</xref>,<xref rid="B175-ijms-18-00020" ref-type="bibr">175</xref>,<xref rid="B176-ijms-18-00020" ref-type="bibr">176</xref>] and macaques [<xref rid="B178-ijms-18-00020" ref-type="bibr">178</xref>]; therefore, they represent excellent LAIV candidates to prevent IAV infections. In addition, a similar approach has been employed to develop attenuated IBVs with similar results [<xref rid="B180-ijms-18-00020" ref-type="bibr">180</xref>]. Mice inoculated with the NS1-truncated or -deleted mutants elicited an antibody response and showed protection against WT virus challenge [<xref rid="B180-ijms-18-00020" ref-type="bibr">180</xref>]. Thus, these NS1-truncated influenza A and B viruses represent excellent candidates as safe, immunogenic and protective LAIVs for multiple virus strains in different animal models.</p></sec><sec id="sec3dot6dot2-ijms-18-00020"><title>3.6.2. Codon-Deoptimized LAIVs</title><p>The genetic code in animals encodes for 20 different amino acids (aa) using 61 codons. This degeneracy of the genetic code allows amino acids, except tryptophan (W) and methionine (M), to be encoded by more than one synonymous codon [<xref rid="B46-ijms-18-00020" ref-type="bibr">46</xref>]. Viruses, including influenza, rely on the host cell translation machinery to synthesize their viral proteins for the formation of infectious virus progeny. As an evolutionary consequence, viruses have modified their codon usage according to the host they infect [<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>]. Experimentally, protein synthesis can be downregulated by synthetically deoptimizing the codon usage of a gene [<xref rid="B141-ijms-18-00020" ref-type="bibr">141</xref>,<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>]. The generation of recombinant viruses containing genes with deoptimized codons is now feasible [<xref rid="B141-ijms-18-00020" ref-type="bibr">141</xref>,<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>], and their level of attenuation depends on the viral gene targeted and the number of codon changes made during the codon deoptimization process [<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>]. For influenza viruses, many regions in the viral genome cannot be altered because of their important role in viral replication and transcription (e.g., NCRs), packaging (e.g., packaging signals), the presence of multiple overlapping ORFs (e.g., segments 7 and 8), etc. [<xref rid="B48-ijms-18-00020" ref-type="bibr">48</xref>]. To date, recombinant IAVs that have been attenuated using a codon-pair [<xref rid="B183-ijms-18-00020" ref-type="bibr">183</xref>,<xref rid="B184-ijms-18-00020" ref-type="bibr">184</xref>] or a codon bias [<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>] deoptimization approach to decrease expression levels of the viral PB1, HA and NP [<xref rid="B183-ijms-18-00020" ref-type="bibr">183</xref>], NA and HA [<xref rid="B184-ijms-18-00020" ref-type="bibr">184</xref>] or NS1 and NEP [<xref rid="B182-ijms-18-00020" ref-type="bibr">182</xref>] have been generated. Importantly, influenza viruses generated by codon deoptimization showed similar viral replication kinetics to WT virus in MDCK cells, which is important for their effective use for vaccine production. However, to date, the ability of these recombinant IAVs containing codon-pair or codon bias deoptimized viral segments to replicate in eggs has not yet been evaluated. Importantly, all of the codon deoptimized IAVs were attenuated in mice and able to provide, upon a single immunization dose, protection against a lethal challenge with a WT form of the virus, showing that LAIVs were safe, immunogenic and protective. However, mice do not accurately reflect virus pathogenesis and immunological responses seen in humans [<xref rid="B81-ijms-18-00020" ref-type="bibr">81</xref>] and do not have the same codon usage bias as humans. Therefore, studies aimed to demonstrate the safety, immunogenicity and protection efficacy of codon deoptimized recombinant IAVs in other well-established animal models of influenza (e.g., guinea pigs, ferrets or nonhuman primates) could lead to their implementation as LAIVs in future vaccinations [<xref rid="B141-ijms-18-00020" ref-type="bibr">141</xref>].</p></sec><sec id="sec3dot6dot3-ijms-18-00020"><title>3.6.3. Single-Cycle Infectious Viruses as LAIVs</title><p>Other promising approaches for the development of LAIVs relate to the use of sciIAVs [<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. SciIAVs based on their safety profile, ability to elicit protective humoral and cellular responses and protective effectiveness represent a feasible alternative to current influenza vaccines for the treatment of influenza viral infections [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. However, to date, no single-cycle infectious IBVs (sciIBV) have been reported. When delivered intranasally, sciIAV has been shown to be safe in a mouse model of influenza infection, without signs of illness or mortality [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B185-ijms-18-00020" ref-type="bibr">185</xref>,<xref rid="B186-ijms-18-00020" ref-type="bibr">186</xref>,<xref rid="B187-ijms-18-00020" ref-type="bibr">187</xref>,<xref rid="B188-ijms-18-00020" ref-type="bibr">188</xref>,<xref rid="B189-ijms-18-00020" ref-type="bibr">189</xref>]. Moreover, and similar to the current LAIVs, intranasal immunization with a single dose of sciIAVs elicited localized mucosal immune responses and recruitment of influenza-specific CD8 T-cells into the lungs of vaccinated animals [<xref rid="B145-ijms-18-00020" ref-type="bibr">145</xref>,<xref rid="B185-ijms-18-00020" ref-type="bibr">185</xref>,<xref rid="B186-ijms-18-00020" ref-type="bibr">186</xref>,<xref rid="B190-ijms-18-00020" ref-type="bibr">190</xref>], the latest being the main contributor of immunity against challenge with heterologous influenza viruses [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B190-ijms-18-00020" ref-type="bibr">190</xref>]. Importantly, sciIAVs protected mice against lethal influenza virus challenges [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. Moreover, similar safety, immunogenicity and protection efficacy of sciIAVs were observed in ferrets [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>]. Vaccination of pigs with sciIAV has also been shown to be immunogenic and protective against challenge with swine influenza viruses [<xref rid="B191-ijms-18-00020" ref-type="bibr">191</xref>]. Moreover, sciIAVs expressing foreign genes represent an excellent option for their implementation as bivalent vaccines. For instance, sciIAVs expressing the surface protein A (PspA) of <italic>Streptococcus pneumoniae</italic>, the hemagglutinin-neuraminidase (HN) protein of HPIV-3 or the fusion (F) protein of respiratory syncytial virus (RSV) [<xref rid="B192-ijms-18-00020" ref-type="bibr">192</xref>,<xref rid="B193-ijms-18-00020" ref-type="bibr">193</xref>,<xref rid="B194-ijms-18-00020" ref-type="bibr">194</xref>], were able to induced Abs against the foreign polypeptides and reduced the viral load of the heterologous pathogen while retaining their ability to protect against challenge with IAV. It is worth indicating that, for vaccine purposes, it is important to consider what influenza viral gene is replaced, since it was shown that sciIAV replication is required for protection [<xref rid="B69-ijms-18-00020" ref-type="bibr">69</xref>,<xref rid="B185-ijms-18-00020" ref-type="bibr">185</xref>,<xref rid="B189-ijms-18-00020" ref-type="bibr">189</xref>]. Although substitution of the polymerase (PB2, PB1 and PA) segments may allow insertion of larger foreign genes, removing the viral polymerase from sciIAVs limits their ability to express more polymerase during the single-cycle round of infection, decreasing total viral antigens and, thus, limiting their protective efficacy. Thus, sciIAVs where the viral HA or NA has been removed were able to confer, upon a single immunization, protection against a lethal challenge with influenza. [<xref rid="B190-ijms-18-00020" ref-type="bibr">190</xref>]. On the contrary, a single dose of a sciIAV where the viral PB2 has been removed was only as efficacious as an inactivated virus [<xref rid="B189-ijms-18-00020" ref-type="bibr">189</xref>]. It is worth noting that sciIAVs combine the advantages (better immunogenic properties of the LAIV and the safety profile of the IIV) and circumvent the disadvantages (safety of the LAIV and poor immunogenicity of the IIV) of current influenza vaccine approaches [<xref rid="B144-ijms-18-00020" ref-type="bibr">144</xref>]. While sciIAVs have been shown to be safe, immunogenic and protective against lethal challenges with wild-type forms of IAVs in animal studies, no human trials have yet been performed.</p></sec><sec id="sec3dot6dot4-ijms-18-00020"><title>3.6.4. LAIVs Based on the Rearrangement of the Influenza Viral Genome</title><p>More recently, the rearrangement of the influenza virus genome has been shown to have great potential for the development of improved LAIVs against influenza virus, as well as vaccine vectors against other pathogens [<xref rid="B195-ijms-18-00020" ref-type="bibr">195</xref>,<xref rid="B196-ijms-18-00020" ref-type="bibr">196</xref>].</p><p>Avian influenza virus subtypes H5N1 and H9N2 have pandemic potential [<xref rid="B197-ijms-18-00020" ref-type="bibr">197</xref>,<xref rid="B198-ijms-18-00020" ref-type="bibr">198</xref>]. However, H5N1 IIV induces limited adaptive immune responses, and in the case of LAIV, there are safety concerns about the possibility of reassortment between the viral segments in the LAIV and circulating H5N1 strains. To overcome these concerns, a bivalent LAIV against influenza A/Vietnam/120320/04 H5N1 and A/Guinea fowl/Hong Kong/WF10/1999 H9N2 was generated using viral genome rearrangement [<xref rid="B195-ijms-18-00020" ref-type="bibr">195</xref>]. To that end, the NEP was removed from the NS viral segment of the H9N2 virus and substituted by the HA of the H5N1 virus. H9N2 NS1 and H5N1 HA were separated by the foot-and-mouth disease virus (FMDV) 2A autocleavage site to allow co-linear expression of both viral proteins. Then, NEP was cloned down-strain of the H9N2 PB1 segment separated by another FMDV 2A autocleavage site. The rearranged H9N2 virus expressing the H5N1 HA was able to provide complete protection against challenge with A/Vietnam/1203/2004 H5N1 and also against a potential pandemic H9:pH1N1 IAV reassortant virus in both mice and ferrets [<xref rid="B195-ijms-18-00020" ref-type="bibr">195</xref>].</p><p>Segments 7 (M) and 8 (NS) of IAVs use an alternative splicing mechanism to express two different viral proteins from the same viral segment. Recently, we have generated recombinant influenza A/Puerto Rico/8/34 H1N1 viruses containing modified M and/or NS segments, in which the overlapping ORFs of the M1 and M2 viral proteins (M segment) and/or the NS1 and NEP proteins (NS segment) were separated with the porcine teschovirus 1 (PTV-1) 2A autocleavage site [<xref rid="B196-ijms-18-00020" ref-type="bibr">196</xref>]. Recombinant viruses with a rearranged M segment were affected or impaired in replication in vitro at nonpermissive temperatures (37 and 39 °C, respectively), whereas high viral titers were obtained at permissive low temperatures (33 °C) [<xref rid="B196-ijms-18-00020" ref-type="bibr">196</xref>]. Notably, viruses containing the M split segment were highly attenuated in vivo, but able to confer, upon a single immunization dose, complete protection against a lethal homologous challenge with wild-type PR8 [<xref rid="B196-ijms-18-00020" ref-type="bibr">196</xref>]. Importantly, viruses with a reorganized M segment were able to confer better protection than a temperature-sensitive, LAIV PR8 virus [<xref rid="B103-ijms-18-00020" ref-type="bibr">103</xref>,<xref rid="B104-ijms-18-00020" ref-type="bibr">104</xref>,<xref rid="B115-ijms-18-00020" ref-type="bibr">115</xref>,<xref rid="B196-ijms-18-00020" ref-type="bibr">196</xref>]. These studies demonstrate that the rearrangement of the influenza viral genome can be used for the generation of safe, immunogenic and protective LAIVs.</p></sec><sec id="sec3dot6dot5-ijms-18-00020"><title>3.6.5. Other Approaches to Generate Influenza Vaccines</title><p>Current influenza vaccines induce immunity to the influenza virus strain-specific HA antigen and are not very effective against new pandemic viruses, given that HA is highly susceptible to frequent changes by antigenic drift and shift [<xref rid="B12-ijms-18-00020" ref-type="bibr">12</xref>,<xref rid="B44-ijms-18-00020" ref-type="bibr">44</xref>,<xref rid="B45-ijms-18-00020" ref-type="bibr">45</xref>,<xref rid="B46-ijms-18-00020" ref-type="bibr">46</xref>,<xref rid="B74-ijms-18-00020" ref-type="bibr">74</xref>,<xref rid="B75-ijms-18-00020" ref-type="bibr">75</xref>,<xref rid="B76-ijms-18-00020" ref-type="bibr">76</xref>]. To overcome these drawbacks, different approaches aimed to develop a universal influenza vaccine able to induce cross-protective broadly neutralizing immunity against conserved viral antigens, such as the ectodomain of M2 (M2e) [<xref rid="B199-ijms-18-00020" ref-type="bibr">199</xref>], the HA stalk domain [<xref rid="B200-ijms-18-00020" ref-type="bibr">200</xref>] or NA [<xref rid="B201-ijms-18-00020" ref-type="bibr">201</xref>], have been explored.</p><p>The M2e antigen is a linear peptide that is very well conserved across IAV strains. Although the mechanism of M2e-specific immunity is unclear, protective anti-M2 antibodies have been observed in multiple animal models, including mice, ferrets and primates [<xref rid="B36-ijms-18-00020" ref-type="bibr">36</xref>]. However, these conserved antigenic targets need to be presented in a carrier system or conjugated to adjuvant molecules.</p><p>Promising results have been obtained with virus-like particles (VLPs), which are morphologically similar to the virus and present surface proteins in a highly immunogenic form. Because VLPs do not contain the viral genome, they are considered safer than viral vaccines, yet still induce strong humoral and cellular immune responses [<xref rid="B202-ijms-18-00020" ref-type="bibr">202</xref>]. VLPs are most commonly made by expression of HA, NA and M1 [<xref rid="B203-ijms-18-00020" ref-type="bibr">203</xref>,<xref rid="B204-ijms-18-00020" ref-type="bibr">204</xref>], although HA and NA alone may be sufficient for VLP production [<xref rid="B205-ijms-18-00020" ref-type="bibr">205</xref>]. Pushko et al. reported the generation of pandemic and seasonal VLPs combining three IAV subtypes. Both the pandemic-subtype VLP (incorporating H5N1, H7N2 and H2N3) and seasonal VLP (A/New Caledonia/20/1999 H1N1, A/New York/55/2004 H3N2 and B/Shanghai/361/2002) induced NAbs and protected ferrets from lethal virus challenges [<xref rid="B206-ijms-18-00020" ref-type="bibr">206</xref>].</p></sec></sec></sec><sec id="sec4-ijms-18-00020"><title>4. Conclusions and Future Direction</title><p>Reverse genetics approaches to generate recombinant viruses, including influenza, have been described for representative family members of negative-sense, single-stranded RNA viruses. These plasmid-based reverse genetics methods have provided scientists with a unique opportunity to study different aspects of the biology and pathogenesis of these viruses, both in vitro and in vivo, as well as to generate attenuated forms to be used as vaccines [<xref rid="B135-ijms-18-00020" ref-type="bibr">135</xref>]. In this review, we discussed the use of reverse genetics for the generation of influenza vaccines with a special focus on LAIVs. In general, LAIVs are highly immunogenic, and immunization usually induces faster and substantially higher levels of both systemic and local mucosal antibody and T-cell responses, providing better protection than their inactivated counterparts. Prevention of influenza virus infection requires seasonal vaccinations, and identification of the correct virus subtype to include in the vaccine leaves little time for vaccine development, scale up and distribution. Although influenza vaccines work well most of the years, if a new viral variant emerges after the strain to be included in the vaccine has been selected, the efficacy of the vaccine would be sub-optimal for this new variant strain. This usually results in decreased efficacy of the vaccine. Reverse genetics approaches have also allowed the development of live-attenuated viruses that could be implemented, in the near future, as LAIVs. These new advances in reverse genetics approaches are reducing the potential time of recovery and production from months to weeks and represent an excellent alternative for the rapid development and implementation of LAIVs for the treatment of both seasonal and potentially pandemic influenza strains.</p></sec></body><back><ack><title>Acknowledgments</title><p>Influenza virus research in the Luis Martínez-Sobrido and Aitor Nogales laboratory was partially funded by the National Institute of Allergy and Infectious Diseases (NIAID) Centers of Excellence for Influenza Research and Surveillance (CEIRS HHSN266200700008C).</p></ack><notes notes-type="COI-statement"><title>Conflicts of Interest</title><p>The authors declare no conflict of interest.</p></notes><glossary><title>Abbreviations</title><array><tbody><tr><td align="left" valign="middle" rowspan="1" colspan="1">IAV</td><td align="left" valign="middle" rowspan="1" colspan="1">Influenza A virus</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">IBV</td><td align="left" valign="middle" rowspan="1" colspan="1">Influenza B virus</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">vRNA</td><td align="left" valign="middle" rowspan="1" colspan="1">Viral RNAs</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">vRNP</td><td align="left" valign="middle" rowspan="1" colspan="1">Viral ribonucleoprotein</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">NAb</td><td align="left" valign="middle" rowspan="1" colspan="1">Neutralizing antibody</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">ts</td><td align="left" valign="middle" rowspan="1" colspan="1">Temperature-sensitive</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">ca</td><td align="left" valign="middle" rowspan="1" colspan="1">Cold-adapted</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">att</td><td align="left" valign="middle" rowspan="1" colspan="1">Attenuated</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">PB2</td><td align="left" valign="middle" rowspan="1" colspan="1">Polymerase basic 2</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">PB1</td><td align="left" valign="middle" rowspan="1" colspan="1">Polymerase basic 1</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">PA</td><td align="left" valign="middle" rowspan="1" colspan="1">Polymerase acid</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">HA</td><td align="left" valign="middle" rowspan="1" colspan="1">Hemagglutinin</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">NA</td><td align="left" valign="middle" rowspan="1" colspan="1">Neuraminidase</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">NP</td><td align="left" valign="middle" rowspan="1" colspan="1">Nucleoprotein</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">NS1</td><td align="left" valign="middle" rowspan="1" colspan="1">Non-structural protein 1</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">ORF</td><td align="left" valign="middle" rowspan="1" colspan="1">Open reading frame</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">NCR</td><td align="left" valign="middle" rowspan="1" colspan="1">Non-coding region</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">IFN-I</td><td align="left" valign="middle" rowspan="1" colspan="1">Type I interferon</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">LAIV</td><td align="left" valign="middle" rowspan="1" colspan="1">Live attenuated influenza vaccine</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">IIV</td><td align="left" valign="middle" rowspan="1" colspan="1">Influenza inactivated vaccine</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">VLP</td><td align="left" valign="middle" rowspan="1" colspan="1">Virus-like particle</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">WHO</td><td align="left" valign="middle" rowspan="1" colspan="1">World Health Organization</td></tr></tbody></array></glossary><ref-list><title>References</title><ref id="B1-ijms-18-00020"><label>1.</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Palese</surname><given-names>P.S.</given-names></name><name><surname>Shaw</surname><given-names>M.L.</given-names></name></person-group><article-title>Orthomyxoviridae: The viruses and their replication</article-title><source>Fields Virology</source><edition>5th ed.</edition><person-group person-group-type="editor"><name><surname>Knipe</surname><given-names>D.M.</given-names></name><name><surname>Howley</surname><given-names>P.M.</given-names></name><name><surname>Griffin</surname><given-names>D.E.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name><name><surname>Martin</surname><given-names>M.A.</given-names></name></person-group><publisher-name>Lippincott Williams & Wilkins</publisher-name><publisher-loc>Philadelphia, PA, USA</publisher-loc><year>2007</year></element-citation></ref><ref id="B2-ijms-18-00020"><label>2.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Steel</surname><given-names>J.</given-names></name><name><surname>Lowen</surname><given-names>A.C.</given-names></name><name><surname>Mubareka</surname><given-names>S.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N</article-title><source>PLoS Pathog.</source><year>2009</year><volume>5</volume><elocation-id>e1000252</elocation-id><pub-id pub-id-type="doi">10.1371/journal.ppat.1000252</pub-id><pub-id pub-id-type="pmid">19119420</pub-id></element-citation></ref><ref id="B3-ijms-18-00020"><label>3.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Parrish</surname><given-names>C.R.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>The origins of new pandemic viruses: The acquisition of new host ranges by canine parvovirus and influenza A viruses</article-title><source>Annu. Rev. Microbiol.</source><year>2005</year><volume>59</volume><fpage>553</fpage><lpage>586</lpage><pub-id pub-id-type="doi">10.1146/annurev.micro.59.030804.121059</pub-id><pub-id pub-id-type="pmid">16153179</pub-id></element-citation></ref><ref id="B4-ijms-18-00020"><label>4.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bean</surname><given-names>W.J.</given-names></name><name><surname>Schell</surname><given-names>M.</given-names></name><name><surname>Katz</surname><given-names>J.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name><name><surname>Naeve</surname><given-names>C.</given-names></name><name><surname>Gorman</surname><given-names>O.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Evolution of the H3 influenza virus hemagglutinin from human and nonhuman hosts</article-title><source>J. Virol.</source><year>1992</year><volume>66</volume><fpage>1129</fpage><lpage>1138</lpage><pub-id pub-id-type="pmid">1731092</pub-id></element-citation></ref><ref id="B5-ijms-18-00020"><label>5.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>R.</given-names></name><name><surname>Holmes</surname><given-names>E.C.</given-names></name></person-group><article-title>The evolutionary dynamics of human influenza B virus</article-title><source>J. Mol. Evol.</source><year>2008</year><volume>66</volume><fpage>655</fpage><lpage>663</lpage><pub-id pub-id-type="doi">10.1007/s00239-008-9119-z</pub-id><pub-id pub-id-type="pmid">18504518</pub-id></element-citation></ref><ref id="B6-ijms-18-00020"><label>6.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Osterhaus</surname><given-names>A.D.</given-names></name><name><surname>Rimmelzwaan</surname><given-names>G.F.</given-names></name><name><surname>Martina</surname><given-names>B.E.</given-names></name><name><surname>Bestebroer</surname><given-names>T.M.</given-names></name><name><surname>Fouchier</surname><given-names>R.A.</given-names></name></person-group><article-title>Influenza B virus in seals</article-title><source>Science</source><year>2000</year><volume>288</volume><fpage>1051</fpage><lpage>1053</lpage><pub-id pub-id-type="doi">10.1126/science.288.5468.1051</pub-id><pub-id pub-id-type="pmid">10807575</pub-id></element-citation></ref><ref id="B7-ijms-18-00020"><label>7.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Resa-Infante</surname><given-names>P.</given-names></name><name><surname>Jorba</surname><given-names>N.</given-names></name><name><surname>Coloma</surname><given-names>R.</given-names></name><name><surname>Ortin</surname><given-names>J.</given-names></name></person-group><article-title>The influenza virus rna synthesis machine: Advances in its structure and function</article-title><source>RNA Biol.</source><year>2011</year><volume>8</volume><fpage>207</fpage><lpage>215</lpage><pub-id pub-id-type="doi">10.4161/rna.8.2.14513</pub-id><pub-id pub-id-type="pmid">21358279</pub-id></element-citation></ref><ref id="B8-ijms-18-00020"><label>8.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Brownlee</surname><given-names>G.G.</given-names></name><name><surname>Fodor</surname><given-names>E.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>Orthomyxovirus replication, transcription, and polyadenylation</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2004</year><volume>283</volume><fpage>121</fpage><lpage>143</lpage><pub-id pub-id-type="pmid">15298169</pub-id></element-citation></ref><ref id="B9-ijms-18-00020"><label>9.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname><given-names>T.S.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name><name><surname>Krystal</surname><given-names>M.</given-names></name></person-group><article-title>Determination 0of influenza virus proteins required for genome replication</article-title><source>J. Virol.</source><year>1990</year><volume>64</volume><fpage>5669</fpage><lpage>5673</lpage><pub-id pub-id-type="pmid">2214032</pub-id></element-citation></ref><ref id="B10-ijms-18-00020"><label>10.</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Wright</surname><given-names>P.</given-names></name><name><surname>Nuemann</surname><given-names>G.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>Orthomhxoviruses</article-title><source>Fields Virology</source><edition>5th ed.</edition><person-group person-group-type="editor"><name><surname>Knipe</surname><given-names>D.M.</given-names></name><name><surname>Howley</surname><given-names>P.M.</given-names></name><name><surname>Griffin</surname><given-names>D.E.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name><name><surname>Martin</surname><given-names>M.A.</given-names></name></person-group><publisher-name>Lippincott Williams & Wilkins</publisher-name><publisher-loc>Philadelphia, PA, USA</publisher-loc><year>2007</year></element-citation></ref><ref id="B11-ijms-18-00020"><label>11.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Stoeckle</surname><given-names>M.Y.</given-names></name><name><surname>Shaw</surname><given-names>M.W.</given-names></name><name><surname>Choppin</surname><given-names>P.W.</given-names></name></person-group><article-title>Segment-specific and common nucleotide sequences in the noncoding regions of influenza B virus genome RNAs</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>1987</year><volume>84</volume><fpage>2703</fpage><lpage>2707</lpage><pub-id pub-id-type="doi">10.1073/pnas.84.9.2703</pub-id><pub-id pub-id-type="pmid">3472232</pub-id></element-citation></ref><ref id="B12-ijms-18-00020"><label>12.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Finch</surname><given-names>C.</given-names></name><name><surname>Tuffy</surname><given-names>K.M.</given-names></name><name><surname>Domm</surname><given-names>W.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Topham</surname><given-names>D.J.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Influenza A and B virus intertypic reassortment through compatible viral packaging signals</article-title><source>J. Virol.</source><year>2014</year><volume>88</volume><fpage>10778</fpage><lpage>10791</lpage><pub-id pub-id-type="doi">10.1128/JVI.01440-14</pub-id><pub-id pub-id-type="pmid">25008914</pub-id></element-citation></ref><ref id="B13-ijms-18-00020"><label>13.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jackson</surname><given-names>D.</given-names></name><name><surname>Elderfield</surname><given-names>R.A.</given-names></name><name><surname>Barclay</surname><given-names>W.S.</given-names></name></person-group><article-title>Molecular studies of influenza B virus in the reverse genetics era</article-title><source>J. Gen. Virol.</source><year>2011</year><volume>92</volume><fpage>1</fpage><lpage>17</lpage><pub-id pub-id-type="doi">10.1099/vir.0.026187-0</pub-id><pub-id pub-id-type="pmid">20926635</pub-id></element-citation></ref><ref id="B14-ijms-18-00020"><label>14.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wise</surname><given-names>H.M.</given-names></name><name><surname>Hutchinson</surname><given-names>E.C.</given-names></name><name><surname>Jagger</surname><given-names>B.W.</given-names></name><name><surname>Stuart</surname><given-names>A.D.</given-names></name><name><surname>Kang</surname><given-names>Z.H.</given-names></name><name><surname>Robb</surname><given-names>N.</given-names></name><name><surname>Schwartzman</surname><given-names>L.M.</given-names></name><name><surname>Kash</surname><given-names>J.C.</given-names></name><name><surname>Fodor</surname><given-names>E.</given-names></name><name><surname>Firth</surname><given-names>A.E.</given-names></name><etal></etal></person-group><article-title>Identification of a novel splice variant form of the influenza A virus M2 ion channel with an antigenically distinct ectodomain</article-title><source>PLoS Pathog.</source><year>2012</year><volume>8</volume><elocation-id>e1002998</elocation-id><pub-id pub-id-type="doi">10.1371/journal.ppat.1002998</pub-id><pub-id pub-id-type="pmid">23133386</pub-id></element-citation></ref><ref id="B15-ijms-18-00020"><label>15.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Horvath</surname><given-names>C.M.</given-names></name><name><surname>Williams</surname><given-names>M.A.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name></person-group><article-title>Eukaryotic coupled tanslation of tandem cistrons: Identification of the influenza B virus BM2 polypeptide</article-title><source>EMBO J.</source><year>1990</year><volume>9</volume><fpage>2639</fpage><lpage>2647</lpage><pub-id pub-id-type="pmid">2114979</pub-id></element-citation></ref><ref id="B16-ijms-18-00020"><label>16.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Skehel</surname><given-names>J.J.</given-names></name><name><surname>Wiley</surname><given-names>D.C.</given-names></name></person-group><article-title>Receptor binding and membrane fusion in virus entry: The influenza hemagglutinin</article-title><source>Annu. Rev. Biochem.</source><year>2000</year><volume>69</volume><fpage>531</fpage><lpage>569</lpage><pub-id pub-id-type="doi">10.1146/annurev.biochem.69.1.531</pub-id><pub-id pub-id-type="pmid">10966468</pub-id></element-citation></ref><ref id="B17-ijms-18-00020"><label>17.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Varghese</surname><given-names>J.N.</given-names></name><name><surname>McKimm-Breschkin</surname><given-names>J.L.</given-names></name><name><surname>Caldwell</surname><given-names>J.B.</given-names></name><name><surname>Kortt</surname><given-names>A.A.</given-names></name><name><surname>Colman</surname><given-names>P.M.</given-names></name></person-group><article-title>The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor</article-title><source>Proteins</source><year>1992</year><volume>14</volume><fpage>327</fpage><lpage>332</lpage><pub-id pub-id-type="doi">10.1002/prot.340140302</pub-id><pub-id pub-id-type="pmid">1438172</pub-id></element-citation></ref><ref id="B18-ijms-18-00020"><label>18.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tulip</surname><given-names>W.R.</given-names></name><name><surname>Varghese</surname><given-names>J.N.</given-names></name><name><surname>Laver</surname><given-names>W.G.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name><name><surname>Colman</surname><given-names>P.M.</given-names></name></person-group><article-title>Refined crystal structure of the influenza virus N9 neuraminidase-NC41 Fab complex</article-title><source>J. Mol. Biol.</source><year>1992</year><volume>227</volume><fpage>122</fpage><lpage>148</lpage><pub-id pub-id-type="doi">10.1016/0022-2836(92)90687-F</pub-id><pub-id pub-id-type="pmid">1381757</pub-id></element-citation></ref><ref id="B19-ijms-18-00020"><label>19.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yoon</surname><given-names>S.W.</given-names></name><name><surname>Webby</surname><given-names>R.J.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Evolution and ecology of influenza A viruses</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2014</year><volume>385</volume><fpage>359</fpage><lpage>375</lpage><pub-id pub-id-type="pmid">24990620</pub-id></element-citation></ref><ref id="B20-ijms-18-00020"><label>20.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tong</surname><given-names>S.</given-names></name><name><surname>Zhu</surname><given-names>X.</given-names></name><name><surname>Li</surname><given-names>Y.</given-names></name><name><surname>Shi</surname><given-names>M.</given-names></name><name><surname>Zhang</surname><given-names>J.</given-names></name><name><surname>Bourgeois</surname><given-names>M.</given-names></name><name><surname>Yang</surname><given-names>H.</given-names></name><name><surname>Chen</surname><given-names>X.</given-names></name><name><surname>Recuenco</surname><given-names>S.</given-names></name><name><surname>Gomez</surname><given-names>J.</given-names></name><etal></etal></person-group><article-title>New world bats harbor diverse influenza A viruses</article-title><source>PLoS Pathog.</source><year>2013</year><volume>9</volume><elocation-id>e1003657</elocation-id><pub-id pub-id-type="doi">10.1371/journal.ppat.1003657</pub-id><pub-id pub-id-type="pmid">24130481</pub-id></element-citation></ref><ref id="B21-ijms-18-00020"><label>21.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McCullers</surname><given-names>J.A.</given-names></name><name><surname>Saito</surname><given-names>T.</given-names></name><name><surname>Iverson</surname><given-names>A.R.</given-names></name></person-group><article-title>Multiple genotypes of influenza B virus circulated between 1979 and 2003</article-title><source>J. Virol.</source><year>2004</year><volume>78</volume><fpage>12817</fpage><lpage>12828</lpage><pub-id pub-id-type="doi">10.1128/JVI.78.23.12817-12828.2004</pub-id><pub-id pub-id-type="pmid">15542634</pub-id></element-citation></ref><ref id="B22-ijms-18-00020"><label>22.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kanegae</surname><given-names>Y.</given-names></name><name><surname>Sugita</surname><given-names>S.</given-names></name><name><surname>Endo</surname><given-names>A.</given-names></name><name><surname>Ishida</surname><given-names>M.</given-names></name><name><surname>Senya</surname><given-names>S.</given-names></name><name><surname>Osako</surname><given-names>K.</given-names></name><name><surname>Nerome</surname><given-names>K.</given-names></name><name><surname>Oya</surname><given-names>A.</given-names></name></person-group><article-title>Evolutionary pattern of the hemagglutinin gene of influenza B viruses isolated in japan: Cocirculating lineages in the same epidemic season</article-title><source>J. Virol.</source><year>1990</year><volume>64</volume><fpage>2860</fpage><lpage>2865</lpage><pub-id pub-id-type="pmid">2335820</pub-id></element-citation></ref><ref id="B23-ijms-18-00020"><label>23.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Thompson</surname><given-names>W.W.</given-names></name><name><surname>Shay</surname><given-names>D.K.</given-names></name><name><surname>Weintraub</surname><given-names>E.</given-names></name><name><surname>Brammer</surname><given-names>L.</given-names></name><name><surname>Cox</surname><given-names>N.</given-names></name><name><surname>Anderson</surname><given-names>L.J.</given-names></name><name><surname>Fukuda</surname><given-names>K.</given-names></name></person-group><article-title>Mortality associated with influenza and respiratory syncytial virus in the united states</article-title><source>JAMA</source><year>2003</year><volume>289</volume><fpage>179</fpage><lpage>186</lpage><pub-id pub-id-type="doi">10.1001/jama.289.2.179</pub-id><pub-id pub-id-type="pmid">12517228</pub-id></element-citation></ref><ref id="B24-ijms-18-00020"><label>24.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>W.C.</given-names></name><name><surname>Shih</surname><given-names>S.R.</given-names></name><name><surname>Huang</surname><given-names>Y.C.</given-names></name><name><surname>Chen</surname><given-names>G.W.</given-names></name><name><surname>Chang</surname><given-names>S.C.</given-names></name><name><surname>Hsiao</surname><given-names>M.J.</given-names></name><name><surname>Tsao</surname><given-names>K.C.</given-names></name><name><surname>Lin</surname><given-names>T.Y.</given-names></name></person-group><article-title>Clinical and genetic characterization of severe influenza B-associated diseases during an outbreak in Taiwan</article-title><source>J. Clin. Virol.</source><year>2008</year><volume>42</volume><fpage>45</fpage><lpage>51</lpage><pub-id pub-id-type="doi">10.1016/j.jcv.2007.11.026</pub-id><pub-id pub-id-type="pmid">18325832</pub-id></element-citation></ref><ref id="B25-ijms-18-00020"><label>25.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hite</surname><given-names>L.K.</given-names></name><name><surname>Glezen</surname><given-names>W.P.</given-names></name><name><surname>Demmler</surname><given-names>G.J.</given-names></name><name><surname>Munoz</surname><given-names>F.M.</given-names></name></person-group><article-title>Medically attended pediatric influenza during the resurgence of the victoria lineage of influenza B virus</article-title><source>Int. J. Infect. Dis.</source><year>2007</year><volume>11</volume><fpage>40</fpage><lpage>47</lpage><pub-id pub-id-type="doi">10.1016/j.ijid.2005.10.008</pub-id><pub-id pub-id-type="pmid">16678464</pub-id></element-citation></ref><ref id="B26-ijms-18-00020"><label>26.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pinto</surname><given-names>L.H.</given-names></name><name><surname>Holsinger</surname><given-names>L.J.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name></person-group><article-title>Influenza virus M2 protein has ion channel activity</article-title><source>Cell</source><year>1992</year><volume>69</volume><fpage>517</fpage><lpage>528</lpage><pub-id pub-id-type="doi">10.1016/0092-8674(92)90452-I</pub-id><pub-id pub-id-type="pmid">1374685</pub-id></element-citation></ref><ref id="B27-ijms-18-00020"><label>27.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lamb</surname><given-names>R.A.</given-names></name><name><surname>Choppin</surname><given-names>P.W.</given-names></name></person-group><article-title>The gene structure and replication of influenza virus</article-title><source>Annu. Rev. Biochem.</source><year>1983</year><volume>52</volume><fpage>467</fpage><lpage>506</lpage><pub-id pub-id-type="doi">10.1146/annurev.bi.52.070183.002343</pub-id><pub-id pub-id-type="pmid">6351727</pub-id></element-citation></ref><ref id="B28-ijms-18-00020"><label>28.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hutchinson</surname><given-names>E.C.</given-names></name><name><surname>von Kirchbach</surname><given-names>J.C.</given-names></name><name><surname>Gog</surname><given-names>J.R.</given-names></name><name><surname>Digard</surname><given-names>P.</given-names></name></person-group><article-title>Genome packaging in influenza A virus</article-title><source>J. Gen. Virol.</source><year>2010</year><volume>91</volume><fpage>313</fpage><lpage>328</lpage><pub-id pub-id-type="doi">10.1099/vir.0.017608-0</pub-id><pub-id pub-id-type="pmid">19955561</pub-id></element-citation></ref><ref id="B29-ijms-18-00020"><label>29.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rossman</surname><given-names>J.S.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name></person-group><article-title>Influenza virus assembly and budding</article-title><source>Virology</source><year>2011</year><volume>411</volume><fpage>229</fpage><lpage>236</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2010.12.003</pub-id><pub-id pub-id-type="pmid">21237476</pub-id></element-citation></ref><ref id="B30-ijms-18-00020"><label>30.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gerber</surname><given-names>M.</given-names></name><name><surname>Isel</surname><given-names>C.</given-names></name><name><surname>Moules</surname><given-names>V.</given-names></name><name><surname>Marquet</surname><given-names>R.</given-names></name></person-group><article-title>Selective packaging of the influenza A genome and consequences for genetic reassortment</article-title><source>Trends Microbiol.</source><year>2014</year><volume>22</volume><fpage>446</fpage><lpage>455</lpage><pub-id pub-id-type="doi">10.1016/j.tim.2014.04.001</pub-id><pub-id pub-id-type="pmid">24798745</pub-id></element-citation></ref><ref id="B31-ijms-18-00020"><label>31.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hale</surname><given-names>B.G.</given-names></name><name><surname>Randall</surname><given-names>R.E.</given-names></name><name><surname>Ortin</surname><given-names>J.</given-names></name><name><surname>Jackson</surname><given-names>D.</given-names></name></person-group><article-title>The multifunctional NS1 protein of influenza A viruses</article-title><source>J. Gen. Virol.</source><year>2008</year><volume>89</volume><fpage>2359</fpage><lpage>2376</lpage><pub-id pub-id-type="doi">10.1099/vir.0.2008/004606-0</pub-id><pub-id pub-id-type="pmid">18796704</pub-id></element-citation></ref><ref id="B32-ijms-18-00020"><label>32.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Paterson</surname><given-names>D.</given-names></name><name><surname>Fodor</surname><given-names>E.</given-names></name></person-group><article-title>Emerging roles for the influenza A virus nuclear export protein (NEP)</article-title><source>PLoS Pathog.</source><year>2012</year><volume>8</volume><elocation-id>e1003019</elocation-id><pub-id pub-id-type="doi">10.1371/journal.ppat.1003019</pub-id><pub-id pub-id-type="pmid">23236273</pub-id></element-citation></ref><ref id="B33-ijms-18-00020"><label>33.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ampofo</surname><given-names>W.K.</given-names></name><name><surname>Baylor</surname><given-names>N.</given-names></name><name><surname>Cobey</surname><given-names>S.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Daves</surname><given-names>S.</given-names></name><name><surname>Edwards</surname><given-names>S.</given-names></name><name><surname>Ferguson</surname><given-names>N.</given-names></name><name><surname>Grohmann</surname><given-names>G.</given-names></name><name><surname>Hay</surname><given-names>A.</given-names></name><name><surname>Katz</surname><given-names>J.</given-names></name><etal></etal></person-group><article-title>Improving influenza vaccine virus selection: Report of a WHO informal consultation held at WHO headquarters, geneva, switzerland, 14–16 June 2010</article-title><source>Influenza Other Respir. Viruses</source><year>2013</year><volume>7</volume><fpage>52</fpage><lpage>53</lpage><pub-id pub-id-type="pmid">24034484</pub-id></element-citation></ref><ref id="B34-ijms-18-00020"><label>34.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Barr</surname><given-names>I.G.</given-names></name><name><surname>McCauley</surname><given-names>J.</given-names></name><name><surname>Cox</surname><given-names>N.</given-names></name><name><surname>Daniels</surname><given-names>R.</given-names></name><name><surname>Engelhardt</surname><given-names>O.G.</given-names></name><name><surname>Fukuda</surname><given-names>K.</given-names></name><name><surname>Grohmann</surname><given-names>G.</given-names></name><name><surname>Hay</surname><given-names>A.</given-names></name><name><surname>Kelso</surname><given-names>A.</given-names></name><name><surname>Klimov</surname><given-names>A.</given-names></name><etal></etal></person-group><article-title>Epidemiological, antigenic and genetic characteristics of seasonal influenza A (H1N1), a(H3N2) and B influenza viruses: Basis for the WHO recommendation on the composition of influenza vaccines for use in the 2009–2010 northern hemisphere season</article-title><source>Vaccine</source><year>2010</year><volume>28</volume><fpage>1156</fpage><lpage>1167</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2009.11.043</pub-id><pub-id pub-id-type="pmid">20004635</pub-id></element-citation></ref><ref id="B35-ijms-18-00020"><label>35.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Brammer</surname><given-names>T.L.</given-names></name><name><surname>Regnery</surname><given-names>H.L.</given-names></name></person-group><article-title>Influenza: Global surveillance for epidemic and pandemic variants</article-title><source>Eur. J. Epidemiol.</source><year>1994</year><volume>10</volume><fpage>467</fpage><lpage>470</lpage><pub-id pub-id-type="doi">10.1007/BF01719678</pub-id><pub-id pub-id-type="pmid">7843358</pub-id></element-citation></ref><ref id="B36-ijms-18-00020"><label>36.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kang</surname><given-names>S.M.</given-names></name><name><surname>Song</surname><given-names>J.M.</given-names></name><name><surname>Compans</surname><given-names>R.W.</given-names></name></person-group><article-title>Novel vaccines against influenza viruses</article-title><source>Virus Res.</source><year>2011</year><volume>162</volume><fpage>31</fpage><lpage>38</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2011.09.037</pub-id><pub-id pub-id-type="pmid">21968298</pub-id></element-citation></ref><ref id="B37-ijms-18-00020"><label>37.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lynch</surname><given-names>J.P.</given-names><suffix>3rd</suffix></name><name><surname>Walsh</surname><given-names>E.E.</given-names></name></person-group><article-title>Influenza: Evolving strategies in treatment and prevention</article-title><source>Semin. Respir. Crit. Care Med.</source><year>2007</year><volume>28</volume><fpage>144</fpage><lpage>158</lpage><pub-id pub-id-type="doi">10.1055/s-2007-976487</pub-id><pub-id pub-id-type="pmid">17458769</pub-id></element-citation></ref><ref id="B38-ijms-18-00020"><label>38.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Molinari</surname><given-names>N.A.</given-names></name><name><surname>Ortega-Sanchez</surname><given-names>I.R.</given-names></name><name><surname>Messonnier</surname><given-names>M.L.</given-names></name><name><surname>Thompson</surname><given-names>W.W.</given-names></name><name><surname>Wortley</surname><given-names>P.M.</given-names></name><name><surname>Weintraub</surname><given-names>E.</given-names></name><name><surname>Bridges</surname><given-names>C.B.</given-names></name></person-group><article-title>The annual impact of seasonal influenza in the us: Measuring disease burden and costs</article-title><source>Vaccine</source><year>2007</year><volume>25</volume><fpage>5086</fpage><lpage>5096</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2007.03.046</pub-id><pub-id pub-id-type="pmid">17544181</pub-id></element-citation></ref><ref id="B39-ijms-18-00020"><label>39.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Girard</surname><given-names>M.P.</given-names></name><name><surname>Cherian</surname><given-names>T.</given-names></name><name><surname>Pervikov</surname><given-names>Y.</given-names></name><name><surname>Kieny</surname><given-names>M.P.</given-names></name></person-group><article-title>A review of vaccine research and development: Human acute respiratory infections</article-title><source>Vaccine</source><year>2005</year><volume>23</volume><fpage>5708</fpage><lpage>5724</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2005.07.046</pub-id><pub-id pub-id-type="pmid">16154667</pub-id></element-citation></ref><ref id="B40-ijms-18-00020"><label>40.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Keech</surname><given-names>M.</given-names></name><name><surname>Beardsworth</surname><given-names>P.</given-names></name></person-group><article-title>The impact of influenza on working days lost: A review of the literature</article-title><source>Pharmacoeconomics</source><year>2008</year><volume>26</volume><fpage>911</fpage><lpage>924</lpage><pub-id pub-id-type="doi">10.2165/00019053-200826110-00004</pub-id><pub-id pub-id-type="pmid">18850761</pub-id></element-citation></ref><ref id="B41-ijms-18-00020"><label>41.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Paul Glezen</surname><given-names>W.</given-names></name><name><surname>Schmier</surname><given-names>J.K.</given-names></name><name><surname>Kuehn</surname><given-names>C.M.</given-names></name><name><surname>Ryan</surname><given-names>K.J.</given-names></name><name><surname>Oxford</surname><given-names>J.</given-names></name></person-group><article-title>The burden of influenza B: A structured literature review</article-title><source>Am. J. Public Health</source><year>2013</year><volume>103</volume><fpage>e43</fpage><lpage>e51</lpage><pub-id pub-id-type="doi">10.2105/AJPH.2012.301137</pub-id><pub-id pub-id-type="pmid">23327249</pub-id></element-citation></ref><ref id="B42-ijms-18-00020"><label>42.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gasparini</surname><given-names>R.</given-names></name><name><surname>Amicizia</surname><given-names>D.</given-names></name><name><surname>Lai</surname><given-names>P.L.</given-names></name><name><surname>Panatto</surname><given-names>D.</given-names></name></person-group><article-title>Clinical and socioeconomic impact of seasonal and pandemic influenza in adults and the elderly</article-title><source>Hum. Vaccines Immunother.</source><year>2012</year><volume>8</volume><fpage>21</fpage><lpage>28</lpage><pub-id pub-id-type="doi">10.4161/hv.8.1.17622</pub-id><pub-id pub-id-type="pmid">22252007</pub-id></element-citation></ref><ref id="B43-ijms-18-00020"><label>43.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smith</surname><given-names>G.J.</given-names></name><name><surname>Vijaykrishna</surname><given-names>D.</given-names></name><name><surname>Bahl</surname><given-names>J.</given-names></name><name><surname>Lycett</surname><given-names>S.J.</given-names></name><name><surname>Worobey</surname><given-names>M.</given-names></name><name><surname>Pybus</surname><given-names>O.G.</given-names></name><name><surname>Ma</surname><given-names>S.K.</given-names></name><name><surname>Cheung</surname><given-names>C.L.</given-names></name><name><surname>Raghwani</surname><given-names>J.</given-names></name><name><surname>Bhatt</surname><given-names>S.</given-names></name><etal></etal></person-group><article-title>Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic</article-title><source>Nature</source><year>2009</year><volume>459</volume><fpage>1122</fpage><lpage>1125</lpage><pub-id pub-id-type="doi">10.1038/nature08182</pub-id><pub-id pub-id-type="pmid">19516283</pub-id></element-citation></ref><ref id="B44-ijms-18-00020"><label>44.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kilbourne</surname><given-names>E.D.</given-names></name></person-group><article-title>Influenza pandemics of the 20th century</article-title><source>Emerg. Infect. Dis.</source><year>2006</year><volume>12</volume><fpage>9</fpage><lpage>14</lpage><pub-id pub-id-type="doi">10.3201/eid1201.051254</pub-id><pub-id pub-id-type="pmid">16494710</pub-id></element-citation></ref><ref id="B45-ijms-18-00020"><label>45.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Carrat</surname><given-names>F.</given-names></name><name><surname>Flahault</surname><given-names>A.</given-names></name></person-group><article-title>Influenza vaccine: The challenge of antigenic drift</article-title><source>Vaccine</source><year>2007</year><volume>25</volume><fpage>6852</fpage><lpage>6862</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2007.07.027</pub-id><pub-id pub-id-type="pmid">17719149</pub-id></element-citation></ref><ref id="B46-ijms-18-00020"><label>46.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Xu</surname><given-names>X.</given-names></name><name><surname>Lindstrom</surname><given-names>S.E.</given-names></name><name><surname>Shaw</surname><given-names>M.W.</given-names></name><name><surname>Smith</surname><given-names>C.B.</given-names></name><name><surname>Hall</surname><given-names>H.E.</given-names></name><name><surname>Mungall</surname><given-names>B.A.</given-names></name><name><surname>Subbarao</surname><given-names>K.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Klimov</surname><given-names>A.</given-names></name></person-group><article-title>Reassortment and evolution of current human influenza A and B viruses</article-title><source>Virus Res.</source><year>2004</year><volume>103</volume><fpage>55</fpage><lpage>60</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2004.02.013</pub-id><pub-id pub-id-type="pmid">15163489</pub-id></element-citation></ref><ref id="B47-ijms-18-00020"><label>47.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sandbulte</surname><given-names>M.R.</given-names></name><name><surname>Westgeest</surname><given-names>K.B.</given-names></name><name><surname>Gao</surname><given-names>J.</given-names></name><name><surname>Xu</surname><given-names>X.</given-names></name><name><surname>Klimov</surname><given-names>A.I.</given-names></name><name><surname>Russell</surname><given-names>C.A.</given-names></name><name><surname>Burke</surname><given-names>D.F.</given-names></name><name><surname>Smith</surname><given-names>D.J.</given-names></name><name><surname>Fouchier</surname><given-names>R.A.</given-names></name><name><surname>Eichelberger</surname><given-names>M.C.</given-names></name></person-group><article-title>Discordant antigenic drift of neuraminidase and hemagglutinin in H1N1 and H3N2 influenza viruses</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2011</year><volume>108</volume><fpage>20748</fpage><lpage>20753</lpage><pub-id pub-id-type="doi">10.1073/pnas.1113801108</pub-id><pub-id pub-id-type="pmid">22143798</pub-id></element-citation></ref><ref id="B48-ijms-18-00020"><label>48.</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Shaw</surname><given-names>M.L.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Orthomyxoviridae</article-title><source>Fields Virology</source><edition>6th ed.</edition><person-group person-group-type="editor"><name><surname>Knipe</surname><given-names>D.M.</given-names></name><name><surname>Howley</surname><given-names>P.M.</given-names></name></person-group><publisher-name>Lippincott Williams & Wilkins</publisher-name><publisher-loc>Philadelphia, PA, USA</publisher-loc><year>2013</year></element-citation></ref><ref id="B49-ijms-18-00020"><label>49.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mikheeva</surname><given-names>A.</given-names></name><name><surname>Ghendon</surname><given-names>Y.Z.</given-names></name></person-group><article-title>Intrinsic interference between influenza A and B viruses</article-title><source>Arch. Virol.</source><year>1982</year><volume>73</volume><fpage>287</fpage><lpage>294</lpage><pub-id pub-id-type="doi">10.1007/BF01318082</pub-id><pub-id pub-id-type="pmid">7149996</pub-id></element-citation></ref><ref id="B50-ijms-18-00020"><label>50.</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Knipe</surname><given-names>D.M.</given-names></name><name><surname>Howley</surname><given-names>P.M.</given-names></name></person-group><source>Fields Virology</source><edition>6th ed.</edition><publisher-name>Wolters Kluwer/Lippincott Williams & Wilkins Health</publisher-name><publisher-loc>Philadelphia, PA, USA</publisher-loc><year>2013</year><fpage>2</fpage></element-citation></ref><ref id="B51-ijms-18-00020"><label>51.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Taubenberger</surname><given-names>J.K.</given-names></name><name><surname>Morens</surname><given-names>D.M.</given-names></name></person-group><article-title>1918 influenza: The mother of all pandemics</article-title><source>Emerg. Infect. Dis.</source><year>2006</year><volume>12</volume><fpage>15</fpage><lpage>22</lpage><pub-id pub-id-type="doi">10.3201/eid1209.05-0979</pub-id><pub-id pub-id-type="pmid">16494711</pub-id></element-citation></ref><ref id="B52-ijms-18-00020"><label>52.</label><element-citation publication-type="journal"><person-group person-group-type="author"><collab>CDC</collab></person-group><article-title>Update: Infections with a swine—Origin influenza A (H1N1) virus—United States and other countries, April 28, 2009</article-title><source>MMWR Morb. Mortal. Wkly. Rep.</source><year>2009</year><volume>58</volume><fpage>431</fpage><lpage>433</lpage><pub-id pub-id-type="pmid">19407737</pub-id></element-citation></ref><ref id="B53-ijms-18-00020"><label>53.</label><element-citation publication-type="webpage"><person-group person-group-type="author"><collab>WHO</collab></person-group><article-title>Pandemic (H1N1) 2009—Update 112</article-title><comment>Available online: <ext-link ext-link-type="uri" xlink:href="http://www.who.int/csr/don/2010_08_06/en/index.html">http://www.who.int/csr/don/2010_08_06/en/index.html</ext-link></comment><date-in-citation>(accessed on 20 December 2016)</date-in-citation></element-citation></ref><ref id="B54-ijms-18-00020"><label>54.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Uyeki</surname><given-names>T.M.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name></person-group><article-title>Global concerns regarding novel influenza A (H7N9) virus infections</article-title><source>N. Engl. J. Med.</source><year>2013</year><volume>368</volume><fpage>1862</fpage><lpage>1864</lpage><pub-id pub-id-type="doi">10.1056/NEJMp1304661</pub-id><pub-id pub-id-type="pmid">23577629</pub-id></element-citation></ref><ref id="B55-ijms-18-00020"><label>55.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yen</surname><given-names>H.L.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Pandemic influenza as a current threat</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2009</year><volume>333</volume><fpage>3</fpage><lpage>24</lpage><pub-id pub-id-type="pmid">19768398</pub-id></element-citation></ref><ref id="B56-ijms-18-00020"><label>56.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Brotherton</surname><given-names>J.M.</given-names></name><name><surname>Delpech</surname><given-names>V.C.</given-names></name><name><surname>Gilbert</surname><given-names>G.L.</given-names></name><name><surname>Hatzi</surname><given-names>S.</given-names></name><name><surname>Paraskevopoulos</surname><given-names>P.D.</given-names></name><name><surname>McAnulty</surname><given-names>J.M.</given-names></name></person-group><article-title>A large outbreak of influenza A and B on a cruise ship causing widespread morbidity</article-title><source>Epidemiol. Infect.</source><year>2003</year><volume>130</volume><fpage>263</fpage><lpage>271</lpage><pub-id pub-id-type="doi">10.1017/S0950268802008166</pub-id><pub-id pub-id-type="pmid">12729195</pub-id></element-citation></ref><ref id="B57-ijms-18-00020"><label>57.</label><element-citation publication-type="journal"><person-group person-group-type="author"><collab>CDC</collab></person-group><article-title>From the centers for disease control and prevention. Influenza B virus outbreak in a cruise ship—Northern europe, 2000</article-title><source>JAMA</source><year>2001</year><volume>285</volume><fpage>1833</fpage><lpage>1834</lpage><pub-id pub-id-type="pmid">11324644</pub-id></element-citation></ref><ref id="B58-ijms-18-00020"><label>58.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>DeStefano</surname><given-names>F.</given-names></name></person-group><article-title>An outbreak of influenza B at an indiana boarding school: Estimate of vaccine efficacy</article-title><source>Public Health Rep.</source><year>1982</year><volume>97</volume><fpage>269</fpage><lpage>272</lpage><pub-id pub-id-type="pmid">7089171</pub-id></element-citation></ref><ref id="B59-ijms-18-00020"><label>59.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fayinka</surname><given-names>O.A.</given-names></name><name><surname>Balayan</surname><given-names>M.S.</given-names></name><name><surname>Kirya</surname><given-names>G.B.</given-names></name><name><surname>Rugyendo</surname><given-names>W.</given-names></name></person-group><article-title>An outbreak of influenza B in a closed community school in Uganda</article-title><source>East. Afr. Med. J.</source><year>1977</year><volume>54</volume><fpage>6</fpage><lpage>8</lpage><pub-id pub-id-type="pmid">852457</pub-id></element-citation></ref><ref id="B60-ijms-18-00020"><label>60.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gordon Smith</surname><given-names>C.E.</given-names></name><name><surname>Thomson</surname><given-names>W.G.</given-names></name></person-group><article-title>An outbreak of influenza due to type B virus in a residential boys’ school in Malaya</article-title><source>Med. J. Malaya</source><year>1956</year><volume>10</volume><fpage>332</fpage><lpage>337</lpage><pub-id pub-id-type="pmid">13399536</pub-id></element-citation></ref><ref id="B61-ijms-18-00020"><label>61.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Osterholm</surname><given-names>M.T.</given-names></name><name><surname>Kelley</surname><given-names>N.S.</given-names></name><name><surname>Sommer</surname><given-names>A.</given-names></name><name><surname>Belongia</surname><given-names>E.A.</given-names></name></person-group><article-title>Efficacy and effectiveness of influenza vaccines: A systematic review and meta-analysis</article-title><source>Lancet Infect. Dis.</source><year>2012</year><volume>12</volume><fpage>36</fpage><lpage>44</lpage><pub-id pub-id-type="doi">10.1016/S1473-3099(11)70295-X</pub-id><pub-id pub-id-type="pmid">22032844</pub-id></element-citation></ref><ref id="B62-ijms-18-00020"><label>62.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>De Jong</surname><given-names>M.D.</given-names></name><name><surname>Tran</surname><given-names>T.T.</given-names></name><name><surname>Truong</surname><given-names>H.K.</given-names></name><name><surname>Vo</surname><given-names>M.H.</given-names></name><name><surname>Smith</surname><given-names>G.J.</given-names></name><name><surname>Nguyen</surname><given-names>V.C.</given-names></name><name><surname>Bach</surname><given-names>V.C.</given-names></name><name><surname>Phan</surname><given-names>T.Q.</given-names></name><name><surname>Do</surname><given-names>Q.H.</given-names></name><name><surname>Guan</surname><given-names>Y.</given-names></name><etal></etal></person-group><article-title>Oseltamivir resistance during treatment of influenza A (H5N1) infection</article-title><source>N. Engl. J. Med.</source><year>2005</year><volume>353</volume><fpage>2667</fpage><lpage>2672</lpage><pub-id pub-id-type="doi">10.1056/NEJMoa054512</pub-id><pub-id pub-id-type="pmid">16371632</pub-id></element-citation></ref><ref id="B63-ijms-18-00020"><label>63.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hurt</surname><given-names>A.C.</given-names></name><name><surname>Holien</surname><given-names>J.K.</given-names></name><name><surname>Parker</surname><given-names>M.</given-names></name><name><surname>Kelso</surname><given-names>A.</given-names></name><name><surname>Barr</surname><given-names>I.G.</given-names></name></person-group><article-title>Zanamivir-resistant influenza viruses with a novel neuraminidase mutation</article-title><source>J. Virol.</source><year>2009</year><volume>83</volume><fpage>10366</fpage><lpage>10373</lpage><pub-id pub-id-type="doi">10.1128/JVI.01200-09</pub-id><pub-id pub-id-type="pmid">19641000</pub-id></element-citation></ref><ref id="B64-ijms-18-00020"><label>64.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Monto</surname><given-names>A.S.</given-names></name><name><surname>McKimm-Breschkin</surname><given-names>J.L.</given-names></name><name><surname>Macken</surname><given-names>C.</given-names></name><name><surname>Hampson</surname><given-names>A.W.</given-names></name><name><surname>Hay</surname><given-names>A.</given-names></name><name><surname>Klimov</surname><given-names>A.</given-names></name><name><surname>Tashiro</surname><given-names>M.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name><name><surname>Aymard</surname><given-names>M.</given-names></name><name><surname>Hayden</surname><given-names>F.G.</given-names></name><etal></etal></person-group><article-title>Detection of influenza viruses resistant to neuraminidase inhibitors in global surveillance during the first 3 years of their use</article-title><source>Antimicrob. Agents Chemother.</source><year>2006</year><volume>50</volume><fpage>2395</fpage><lpage>2402</lpage><pub-id pub-id-type="doi">10.1128/AAC.01339-05</pub-id><pub-id pub-id-type="pmid">16801417</pub-id></element-citation></ref><ref id="B65-ijms-18-00020"><label>65.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Belshe</surname><given-names>R.B.</given-names></name><name><surname>Edwards</surname><given-names>K.M.</given-names></name><name><surname>Vesikari</surname><given-names>T.</given-names></name><name><surname>Black</surname><given-names>S.V.</given-names></name><name><surname>Walker</surname><given-names>R.E.</given-names></name><name><surname>Hultquist</surname><given-names>M.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name><name><surname>Connor</surname><given-names>E.M.</given-names></name></person-group><article-title>Live attenuated versus inactivated influenza vaccine in infants and young children</article-title><source>N. Engl. J. Med.</source><year>2007</year><volume>356</volume><fpage>685</fpage><lpage>696</lpage><pub-id pub-id-type="doi">10.1056/NEJMoa065368</pub-id><pub-id pub-id-type="pmid">17301299</pub-id></element-citation></ref><ref id="B66-ijms-18-00020"><label>66.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Belshe</surname><given-names>R.B.</given-names></name><name><surname>Newman</surname><given-names>F.K.</given-names></name><name><surname>Wilkins</surname><given-names>K.</given-names></name><name><surname>Graham</surname><given-names>I.L.</given-names></name><name><surname>Babusis</surname><given-names>E.</given-names></name><name><surname>Ewell</surname><given-names>M.</given-names></name><name><surname>Frey</surname><given-names>S.E.</given-names></name></person-group><article-title>Comparative immunogenicity of trivalent influenza vaccine administered by intradermal or intramuscular route in healthy adults</article-title><source>Vaccine</source><year>2007</year><volume>25</volume><fpage>6755</fpage><lpage>6763</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2007.06.066</pub-id><pub-id pub-id-type="pmid">17692438</pub-id></element-citation></ref><ref id="B67-ijms-18-00020"><label>67.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pronker</surname><given-names>E.S.</given-names></name><name><surname>Claassen</surname><given-names>E.</given-names></name><name><surname>Osterhaus</surname><given-names>A.D.</given-names></name></person-group><article-title>Development of new generation influenza vaccines: Recipes for success?</article-title><source>Vaccine</source><year>2012</year><volume>30</volume><fpage>7344</fpage><lpage>7347</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2012.09.071</pub-id><pub-id pub-id-type="pmid">23044407</pub-id></element-citation></ref><ref id="B68-ijms-18-00020"><label>68.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cox</surname><given-names>M.M.</given-names></name><name><surname>Patriarca</surname><given-names>P.A.</given-names></name><name><surname>Treanor</surname><given-names>J.</given-names></name></person-group><article-title>Flublok, a recombinant hemagglutinin influenza vaccine</article-title><source>Influenza. Other Respir. Viruses</source><year>2008</year><volume>2</volume><fpage>211</fpage><lpage>219</lpage><pub-id pub-id-type="doi">10.1111/j.1750-2659.2008.00053.x</pub-id><pub-id pub-id-type="pmid">19453397</pub-id></element-citation></ref><ref id="B69-ijms-18-00020"><label>69.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Guo</surname><given-names>H.</given-names></name><name><surname>Albrecht</surname><given-names>R.A.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Topham</surname><given-names>D.J.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Protection against lethal influenza with a viral mimic</article-title><source>J. Virol.</source><year>2013</year><volume>87</volume><fpage>8591</fpage><lpage>8605</lpage><pub-id pub-id-type="doi">10.1128/JVI.01081-13</pub-id><pub-id pub-id-type="pmid">23720727</pub-id></element-citation></ref><ref id="B70-ijms-18-00020"><label>70.</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Sun</surname><given-names>W.</given-names></name></person-group><source>Approval Letter—Fluarix Quadrivalent (BL 125127/513)</source><publisher-name>U.S. Food and Drug Administration (FDA)</publisher-name><publisher-loc>Silver Spring, MD, USA</publisher-loc><year>2012</year></element-citation></ref><ref id="B71-ijms-18-00020"><label>71.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Belongia</surname><given-names>E.A.</given-names></name><name><surname>Kieke</surname><given-names>B.A.</given-names></name><name><surname>Donahue</surname><given-names>J.G.</given-names></name><name><surname>Greenlee</surname><given-names>R.T.</given-names></name><name><surname>Balish</surname><given-names>A.</given-names></name><name><surname>Foust</surname><given-names>A.</given-names></name><name><surname>Lindstrom</surname><given-names>S.</given-names></name><name><surname>Shay</surname><given-names>D.K.</given-names></name></person-group><article-title>Effectiveness of inactivated influenza vaccines varied substantially with antigenic match from the 2004–2005 season to the 2006–2007 season</article-title><source>J. Infect. Dis.</source><year>2009</year><volume>199</volume><fpage>159</fpage><lpage>167</lpage><pub-id pub-id-type="doi">10.1086/595861</pub-id><pub-id pub-id-type="pmid">19086915</pub-id></element-citation></ref><ref id="B72-ijms-18-00020"><label>72.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pica</surname><given-names>N.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Toward a universal influenza virus vaccine: Prospects and challenges</article-title><source>Annu. Rev. Med.</source><year>2013</year><volume>64</volume><fpage>189</fpage><lpage>202</lpage><pub-id pub-id-type="doi">10.1146/annurev-med-120611-145115</pub-id><pub-id pub-id-type="pmid">23327522</pub-id></element-citation></ref><ref id="B73-ijms-18-00020"><label>73.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gorse</surname><given-names>G.J.</given-names></name><name><surname>Belshe</surname><given-names>R.B.</given-names></name><name><surname>Munn</surname><given-names>N.J.</given-names></name></person-group><article-title>Superiority of live-attenuated compared with inactivated influenza A virus vaccines in older, chronically ill adults</article-title><source>Chest</source><year>1991</year><volume>100</volume><fpage>977</fpage><lpage>984</lpage><pub-id pub-id-type="doi">10.1378/chest.100.4.977</pub-id><pub-id pub-id-type="pmid">1914615</pub-id></element-citation></ref><ref id="B74-ijms-18-00020"><label>74.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Antiviral response in pandemic influenza viruses</article-title><source>Emerg. Infect. Dis.</source><year>2006</year><volume>12</volume><fpage>44</fpage><lpage>47</lpage><pub-id pub-id-type="doi">10.3201/eid1201.051186</pub-id><pub-id pub-id-type="pmid">16494716</pub-id></element-citation></ref><ref id="B75-ijms-18-00020"><label>75.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Oxford</surname><given-names>J.S.</given-names></name><name><surname>Bossuyt</surname><given-names>S.</given-names></name><name><surname>Balasingam</surname><given-names>S.</given-names></name><name><surname>Mann</surname><given-names>A.</given-names></name><name><surname>Novelli</surname><given-names>P.</given-names></name><name><surname>Lambkin</surname><given-names>R.</given-names></name></person-group><article-title>Treatment of epidemic and pandemic influenza with neuraminidase and M2 proton channel inhibitors</article-title><source>Clin. Microbiol. Infect.</source><year>2003</year><volume>9</volume><fpage>1</fpage><lpage>14</lpage><pub-id pub-id-type="doi">10.1046/j.1469-0691.2003.00564.x</pub-id><pub-id pub-id-type="pmid">12691538</pub-id></element-citation></ref><ref id="B76-ijms-18-00020"><label>76.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Seibert</surname><given-names>C.W.</given-names></name><name><surname>Kaminski</surname><given-names>M.</given-names></name><name><surname>Philipp</surname><given-names>J.</given-names></name><name><surname>Rubbenstroth</surname><given-names>D.</given-names></name><name><surname>Albrecht</surname><given-names>R.A.</given-names></name><name><surname>Schwalm</surname><given-names>F.</given-names></name><name><surname>Stertz</surname><given-names>S.</given-names></name><name><surname>Medina</surname><given-names>R.A.</given-names></name><name><surname>Kochs</surname><given-names>G.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><etal></etal></person-group><article-title>Oseltamivir-resistant variants of the 2009 pandemic H1N1 influenza A virus are not attenuated in the guinea pig and ferret transmission models</article-title><source>J. Virol.</source><year>2010</year><volume>84</volume><fpage>11219</fpage><lpage>11226</lpage><pub-id pub-id-type="doi">10.1128/JVI.01424-10</pub-id><pub-id pub-id-type="pmid">20739532</pub-id></element-citation></ref><ref id="B77-ijms-18-00020"><label>77.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jackson</surname><given-names>R.J.</given-names></name><name><surname>Cooper</surname><given-names>K.L.</given-names></name><name><surname>Tappenden</surname><given-names>P.</given-names></name><name><surname>Rees</surname><given-names>A.</given-names></name><name><surname>Simpson</surname><given-names>E.L.</given-names></name><name><surname>Read</surname><given-names>R.C.</given-names></name><name><surname>Nicholson</surname><given-names>K.G.</given-names></name></person-group><article-title>Oseltamivir, zanamivir and amantadine in the prevention of influenza: A systematic review</article-title><source>J. Infect.</source><year>2011</year><volume>62</volume><fpage>14</fpage><lpage>25</lpage><pub-id pub-id-type="doi">10.1016/j.jinf.2010.10.003</pub-id><pub-id pub-id-type="pmid">20950645</pub-id></element-citation></ref><ref id="B78-ijms-18-00020"><label>78.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nguyen</surname><given-names>J.T.</given-names></name><name><surname>Hoopes</surname><given-names>J.D.</given-names></name><name><surname>Le</surname><given-names>M.H.</given-names></name><name><surname>Smee</surname><given-names>D.F.</given-names></name><name><surname>Patick</surname><given-names>A.K.</given-names></name><name><surname>Faix</surname><given-names>D.J.</given-names></name><name><surname>Blair</surname><given-names>P.J.</given-names></name><name><surname>de Jong</surname><given-names>M.D.</given-names></name><name><surname>Prichard</surname><given-names>M.N.</given-names></name><name><surname>Went</surname><given-names>G.T.</given-names></name></person-group><article-title>Triple combination of amantadine, ribavirin, and oseltamivir is highly active and synergistic against drug resistant influenza virus strains in vitro</article-title><source>PLoS ONE</source><year>2010</year><volume>5</volume><elocation-id>e9332</elocation-id><pub-id pub-id-type="doi">10.1371/journal.pone.0009332</pub-id><pub-id pub-id-type="pmid">20179772</pub-id></element-citation></ref><ref id="B79-ijms-18-00020"><label>79.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hay</surname><given-names>A.J.</given-names></name><name><surname>Wolstenholme</surname><given-names>A.J.</given-names></name><name><surname>Skehel</surname><given-names>J.J.</given-names></name><name><surname>Smith</surname><given-names>M.H.</given-names></name></person-group><article-title>The molecular basis of the specific anti-influenza action of amantadine</article-title><source>EMBO J.</source><year>1985</year><volume>4</volume><fpage>3021</fpage><lpage>3024</lpage><pub-id pub-id-type="pmid">4065098</pub-id></element-citation></ref><ref id="B80-ijms-18-00020"><label>80.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gubareva</surname><given-names>L.V.</given-names></name><name><surname>Kaiser</surname><given-names>L.</given-names></name><name><surname>Hayden</surname><given-names>F.G.</given-names></name></person-group><article-title>Influenza virus neuraminidase inhibitors</article-title><source>Lancet</source><year>2000</year><volume>355</volume><fpage>827</fpage><lpage>835</lpage><pub-id pub-id-type="doi">10.1016/S0140-6736(99)11433-8</pub-id><pub-id pub-id-type="pmid">10711940</pub-id></element-citation></ref><ref id="B81-ijms-18-00020"><label>81.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Burnham</surname><given-names>A.J.</given-names></name><name><surname>Baranovich</surname><given-names>T.</given-names></name><name><surname>Govorkova</surname><given-names>E.A.</given-names></name></person-group><article-title>Neuraminidase inhibitors for influenza B virus infection: Efficacy and resistance</article-title><source>Antivir. Res.</source><year>2013</year><volume>100</volume><fpage>520</fpage><lpage>534</lpage><pub-id pub-id-type="doi">10.1016/j.antiviral.2013.08.023</pub-id><pub-id pub-id-type="pmid">24013000</pub-id></element-citation></ref><ref id="B82-ijms-18-00020"><label>82.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Farrukee</surname><given-names>R.</given-names></name><name><surname>Mosse</surname><given-names>J.</given-names></name><name><surname>Hurt</surname><given-names>A.C.</given-names></name></person-group><article-title>Review of the clinical effectiveness of the neuraminidase inhibitors against influenza B viruses</article-title><source>Expert Rev. Anti Infect. Ther.</source><year>2013</year><volume>11</volume><fpage>1135</fpage><lpage>1145</lpage><pub-id pub-id-type="doi">10.1586/14787210.2013.842466</pub-id><pub-id pub-id-type="pmid">24093683</pub-id></element-citation></ref><ref id="B83-ijms-18-00020"><label>83.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pizzorno</surname><given-names>A.</given-names></name><name><surname>Abed</surname><given-names>Y.</given-names></name><name><surname>Boivin</surname><given-names>G.</given-names></name></person-group><article-title>Influenza drug resistance</article-title><source>Semin. Respir. Crit. Care Med.</source><year>2011</year><volume>32</volume><fpage>409</fpage><lpage>422</lpage><pub-id pub-id-type="doi">10.1055/s-0031-1283281</pub-id><pub-id pub-id-type="pmid">21858746</pub-id></element-citation></ref><ref id="B84-ijms-18-00020"><label>84.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nguyen</surname><given-names>H.T.</given-names></name><name><surname>Sheu</surname><given-names>T.G.</given-names></name><name><surname>Mishin</surname><given-names>V.P.</given-names></name><name><surname>Klimov</surname><given-names>A.I.</given-names></name><name><surname>Gubareva</surname><given-names>L.V.</given-names></name></person-group><article-title>Assessment of pandemic and seasonal influenza A (H1N1) virus susceptibility to neuraminidase inhibitors in three enzyme activity inhibition assays</article-title><source>Antimicrob. Agents Chemother.</source><year>2010</year><volume>54</volume><fpage>3671</fpage><lpage>3677</lpage><pub-id pub-id-type="doi">10.1128/AAC.00581-10</pub-id><pub-id pub-id-type="pmid">20585136</pub-id></element-citation></ref><ref id="B85-ijms-18-00020"><label>85.</label><element-citation publication-type="confproc"><person-group person-group-type="author"><name><surname>Ampofo</surname><given-names>W.K.</given-names></name><name><surname>Al Busaidy</surname><given-names>S.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Giovanni</surname><given-names>M.</given-names></name><name><surname>Hay</surname><given-names>A.</given-names></name><name><surname>Huang</surname><given-names>S.</given-names></name><name><surname>Inglis</surname><given-names>S.</given-names></name><name><surname>Katz</surname><given-names>J.</given-names></name><name><surname>Mokhtari-Azad</surname><given-names>T.</given-names></name><name><surname>Peiris</surname><given-names>M.</given-names></name><etal></etal></person-group><article-title>Strengthening the influenza vaccine virus selection and development process</article-title><source>Presented at the 2nd WHO Informal Consultation for Improving Influenza Vaccine Virus Selection</source><conf-loc>Geneva, Switzerland</conf-loc><conf-date>7–9 December 2011</conf-date></element-citation></ref><ref id="B86-ijms-18-00020"><label>86.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Russell</surname><given-names>C.A.</given-names></name><name><surname>Jones</surname><given-names>T.C.</given-names></name><name><surname>Barr</surname><given-names>I.G.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Garten</surname><given-names>R.J.</given-names></name><name><surname>Gregory</surname><given-names>V.</given-names></name><name><surname>Gust</surname><given-names>I.D.</given-names></name><name><surname>Hampson</surname><given-names>A.W.</given-names></name><name><surname>Hay</surname><given-names>A.J.</given-names></name><name><surname>Hurt</surname><given-names>A.C.</given-names></name><etal></etal></person-group><article-title>Influenza vaccine strain selection and recent studies on the global migration of seasonal influenza viruses</article-title><source>Vaccine</source><year>2008</year><volume>26</volume><fpage>D31</fpage><lpage>D34</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2008.07.078</pub-id><pub-id pub-id-type="pmid">19230156</pub-id></element-citation></ref><ref id="B87-ijms-18-00020"><label>87.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Liang</surname><given-names>S.</given-names></name><name><surname>Mozdzanowska</surname><given-names>K.</given-names></name><name><surname>Palladino</surname><given-names>G.</given-names></name><name><surname>Gerhard</surname><given-names>W.</given-names></name></person-group><article-title>Heterosubtypic immunity to influenza type A virus in mice. Effector mechanisms and their longevity</article-title><source>J. Immunol.</source><year>1994</year><volume>152</volume><fpage>1653</fpage><lpage>1661</lpage><pub-id pub-id-type="pmid">8120375</pub-id></element-citation></ref><ref id="B88-ijms-18-00020"><label>88.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Medina</surname><given-names>R.A.</given-names></name><name><surname>Manicassamy</surname><given-names>B.</given-names></name><name><surname>Stertz</surname><given-names>S.</given-names></name><name><surname>Seibert</surname><given-names>C.W.</given-names></name><name><surname>Hai</surname><given-names>R.</given-names></name><name><surname>Belshe</surname><given-names>R.B.</given-names></name><name><surname>Frey</surname><given-names>S.E.</given-names></name><name><surname>Basler</surname><given-names>C.F.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Pandemic 2009 H1N1 vaccine protects against 1918 Spanish influenza virus</article-title><source>Nat. Commun.</source><year>2010</year><volume>1</volume><fpage>28</fpage><pub-id pub-id-type="doi">10.1038/ncomms1026</pub-id><pub-id pub-id-type="pmid">20975689</pub-id></element-citation></ref><ref id="B89-ijms-18-00020"><label>89.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Krammer</surname><given-names>F.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name><name><surname>Steel</surname><given-names>J.</given-names></name></person-group><article-title>Advances in universal influenza virus vaccine design and antibody mediated therapies based on conserved regions of the hemagglutinin</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2015</year><volume>386</volume><fpage>301</fpage><lpage>321</lpage><pub-id pub-id-type="pmid">25007847</pub-id></element-citation></ref><ref id="B90-ijms-18-00020"><label>90.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Milian</surname><given-names>E.</given-names></name><name><surname>Kamen</surname><given-names>A.A.</given-names></name></person-group><article-title>Current and emerging cell culture manufacturing technologies for influenza vaccines</article-title><source>BioMed Res. Int.</source><year>2015</year><volume>2015</volume><fpage>504831</fpage><pub-id pub-id-type="doi">10.1155/2015/504831</pub-id><pub-id pub-id-type="pmid">25815321</pub-id></element-citation></ref><ref id="B91-ijms-18-00020"><label>91.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Katz</surname><given-names>J.M.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Efficacy of inactivated influenza A virus (H3N2) vaccines grown in mammalian cells or embryonated eggs</article-title><source>J. Infect. Dis.</source><year>1989</year><volume>160</volume><fpage>191</fpage><lpage>198</lpage><pub-id pub-id-type="doi">10.1093/infdis/160.2.191</pub-id><pub-id pub-id-type="pmid">2760480</pub-id></element-citation></ref><ref id="B92-ijms-18-00020"><label>92.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Meyer</surname><given-names>W.J.</given-names></name><name><surname>Wood</surname><given-names>J.M.</given-names></name><name><surname>Major</surname><given-names>D.</given-names></name><name><surname>Robertson</surname><given-names>J.S.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name><name><surname>Katz</surname><given-names>J.M.</given-names></name></person-group><article-title>Influence of host cell-mediated variation on the international surveillance of influenza A (H3N2) viruses</article-title><source>Virology</source><year>1993</year><volume>196</volume><fpage>130</fpage><lpage>137</lpage><pub-id pub-id-type="doi">10.1006/viro.1993.1461</pub-id><pub-id pub-id-type="pmid">8356790</pub-id></element-citation></ref><ref id="B93-ijms-18-00020"><label>93.</label><element-citation publication-type="confproc"><person-group person-group-type="author"><name><surname>Ampofo</surname><given-names>W.K.</given-names></name><name><surname>Azziz-Baumgartner</surname><given-names>E.</given-names></name><name><surname>Bashir</surname><given-names>U.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Fasce</surname><given-names>R.</given-names></name><name><surname>Giovanni</surname><given-names>M.</given-names></name><name><surname>Grohmann</surname><given-names>G.</given-names></name><name><surname>Huang</surname><given-names>S.</given-names></name><name><surname>Katz</surname><given-names>J.</given-names></name><name><surname>Mironenko</surname><given-names>A.</given-names></name><etal></etal></person-group><article-title>Strengthening the influenza vaccine virus selection and development process</article-title><source>Presented at the 3rd WHO Informal Consultation for Improving Influenza Vaccine Virus Selection</source><conf-loc>Geneva, Switzerland</conf-loc><conf-date>1–3 April 2014</conf-date></element-citation></ref><ref id="B94-ijms-18-00020"><label>94.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Salk</surname><given-names>J.</given-names></name><name><surname>Salk</surname><given-names>D.</given-names></name></person-group><article-title>Control of influenza and poliomyelitis with killed virus vaccines</article-title><source>Science</source><year>1977</year><volume>195</volume><fpage>834</fpage><lpage>847</lpage><pub-id pub-id-type="doi">10.1126/science.320661</pub-id><pub-id pub-id-type="pmid">320661</pub-id></element-citation></ref><ref id="B95-ijms-18-00020"><label>95.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>De Villiers</surname><given-names>P.J.</given-names></name><name><surname>Steele</surname><given-names>A.D.</given-names></name><name><surname>Hiemstra</surname><given-names>L.A.</given-names></name><name><surname>Rappaport</surname><given-names>R.</given-names></name><name><surname>Dunning</surname><given-names>A.J.</given-names></name><name><surname>Gruber</surname><given-names>W.C.</given-names></name><name><surname>Forrest</surname><given-names>B.D.</given-names></name></person-group><article-title>Efficacy and safety of a live-attenuated influenza vaccine in adults 60 years of age and older</article-title><source>Vaccine</source><year>2009</year><volume>28</volume><fpage>228</fpage><lpage>234</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2009.09.092</pub-id><pub-id pub-id-type="pmid">19796721</pub-id></element-citation></ref><ref id="B96-ijms-18-00020"><label>96.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tlaxca</surname><given-names>J.L.</given-names></name><name><surname>Ellis</surname><given-names>S.</given-names></name><name><surname>Remmele</surname><given-names>R.L.</given-names><suffix>Jr.</suffix></name></person-group><article-title>Live attenuated and inactivated viral vaccine formulation and nasal delivery: Potential and challenges</article-title><source>Adv. Drug. Deliv. Rev.</source><year>2015</year><volume>93</volume><fpage>56</fpage><lpage>78</lpage><pub-id pub-id-type="doi">10.1016/j.addr.2014.10.002</pub-id><pub-id pub-id-type="pmid">25312673</pub-id></element-citation></ref><ref id="B97-ijms-18-00020"><label>97.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Laver</surname><given-names>W.G.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Preparation and immunogenicity of an influenza virus hemagglutinin and neuraminidase subunit vaccine</article-title><source>Virology</source><year>1976</year><volume>69</volume><fpage>511</fpage><lpage>522</lpage><pub-id pub-id-type="doi">10.1016/0042-6822(76)90481-5</pub-id><pub-id pub-id-type="pmid">1258359</pub-id></element-citation></ref><ref id="B98-ijms-18-00020"><label>98.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wong</surname><given-names>S.S.</given-names></name><name><surname>Webby</surname><given-names>R.J.</given-names></name></person-group><article-title>Traditional and new influenza vaccines</article-title><source>Clin. Microbiol. Rev.</source><year>2013</year><volume>26</volume><fpage>476</fpage><lpage>492</lpage><pub-id pub-id-type="doi">10.1128/CMR.00097-12</pub-id><pub-id pub-id-type="pmid">23824369</pub-id></element-citation></ref><ref id="B99-ijms-18-00020"><label>99.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Duxbury</surname><given-names>A.E.</given-names></name><name><surname>Hampson</surname><given-names>A.W.</given-names></name><name><surname>Sievers</surname><given-names>J.G.</given-names></name></person-group><article-title>Antibody response in humans to deoxycholate-treated influenza virus vaccine</article-title><source>J. Immunol.</source><year>1968</year><volume>101</volume><fpage>62</fpage><lpage>67</lpage><pub-id pub-id-type="pmid">5662705</pub-id></element-citation></ref><ref id="B100-ijms-18-00020"><label>100.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baxter</surname><given-names>R.</given-names></name><name><surname>Patriarca</surname><given-names>P.A.</given-names></name><name><surname>Ensor</surname><given-names>K.</given-names></name><name><surname>Izikson</surname><given-names>R.</given-names></name><name><surname>Goldenthal</surname><given-names>K.L.</given-names></name><name><surname>Cox</surname><given-names>M.M.</given-names></name></person-group><article-title>Evaluation of the safety, reactogenicity and immunogenicity of flublok(r) trivalent recombinant baculovirus-expressed hemagglutinin influenza vaccine administered intramuscularly to healthy adults 50–64 years of age</article-title><source>Vaccine</source><year>2011</year><volume>29</volume><fpage>2272</fpage><lpage>2278</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2011.01.039</pub-id><pub-id pub-id-type="pmid">21277410</pub-id></element-citation></ref><ref id="B101-ijms-18-00020"><label>101.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Miller</surname><given-names>G.L.</given-names></name></person-group><article-title>A study of conditions for the optimum production of PR8 influenza virus in chick embryos</article-title><source>J. Exp. Med.</source><year>1944</year><volume>79</volume><fpage>173</fpage><lpage>183</lpage><pub-id pub-id-type="doi">10.1084/jem.79.2.173</pub-id><pub-id pub-id-type="pmid">19871361</pub-id></element-citation></ref><ref id="B102-ijms-18-00020"><label>102.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Maassab</surname><given-names>H.F.</given-names></name><name><surname>Bryant</surname><given-names>M.L.</given-names></name></person-group><article-title>The development of live-attenuated cold-adapted influenza virus vaccine for humans</article-title><source>Rev. Med. Virol.</source><year>1999</year><volume>9</volume><fpage>237</fpage><lpage>244</lpage><pub-id pub-id-type="doi">10.1002/(SICI)1099-1654(199910/12)9:4<237::AID-RMV252>3.0.CO;2-G</pub-id><pub-id pub-id-type="pmid">10578119</pub-id></element-citation></ref><ref id="B103-ijms-18-00020"><label>103.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Snyder</surname><given-names>M.H.</given-names></name><name><surname>Betts</surname><given-names>R.F.</given-names></name><name><surname>DeBorde</surname><given-names>D.</given-names></name><name><surname>Tierney</surname><given-names>E.L.</given-names></name><name><surname>Clements</surname><given-names>M.L.</given-names></name><name><surname>Herrington</surname><given-names>D.</given-names></name><name><surname>Sears</surname><given-names>S.D.</given-names></name><name><surname>Dolin</surname><given-names>R.</given-names></name><name><surname>Maassab</surname><given-names>H.F.</given-names></name><name><surname>Murphy</surname><given-names>B.R.</given-names></name></person-group><article-title>Four viral genes independently contribute to attenuation of live influenza A/Ann Arbor/6/60 (H2N2) cold-adapted reassortant virus vaccines</article-title><source>J. Virol.</source><year>1988</year><volume>62</volume><fpage>488</fpage><lpage>495</lpage><pub-id pub-id-type="pmid">3336068</pub-id></element-citation></ref><ref id="B104-ijms-18-00020"><label>104.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cox</surname><given-names>N.J.</given-names></name><name><surname>Kitame</surname><given-names>F.</given-names></name><name><surname>Kendal</surname><given-names>A.P.</given-names></name><name><surname>Maassab</surname><given-names>H.F.</given-names></name><name><surname>Naeve</surname><given-names>C.</given-names></name></person-group><article-title>Identification of sequence changes in the cold-adapted, live-attenuated influenza vaccine strain, A/Ann Arbor/6/60 (H2N2)</article-title><source>Virology</source><year>1988</year><volume>167</volume><fpage>554</fpage><lpage>567</lpage><pub-id pub-id-type="doi">10.1016/0042-6822(88)90118-3</pub-id><pub-id pub-id-type="pmid">2974219</pub-id></element-citation></ref><ref id="B105-ijms-18-00020"><label>105.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Maassab</surname><given-names>H.F.</given-names></name></person-group><article-title>Plaque formation of influenza virus at 25 °C</article-title><source>Nature</source><year>1968</year><volume>219</volume><fpage>645</fpage><lpage>646</lpage><pub-id pub-id-type="doi">10.1038/219645a0</pub-id><pub-id pub-id-type="pmid">5665725</pub-id></element-citation></ref><ref id="B106-ijms-18-00020"><label>106.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Couch</surname><given-names>R.B.</given-names></name><name><surname>Keitel</surname><given-names>W.A.</given-names></name><name><surname>Cate</surname><given-names>T.R.</given-names></name></person-group><article-title>Improvement of inactivated influenza virus vaccines</article-title><source>J. Infect. Dis.</source><year>1997</year><volume>176</volume><fpage>S38</fpage><lpage>S44</lpage><pub-id pub-id-type="doi">10.1086/514173</pub-id><pub-id pub-id-type="pmid">9240693</pub-id></element-citation></ref><ref id="B107-ijms-18-00020"><label>107.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>Z.</given-names></name><name><surname>Aspelund</surname><given-names>A.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name><name><surname>Jin</surname><given-names>H.</given-names></name></person-group><article-title>Molecular studies of temperature-sensitive replication of the cold-adapted B/Ann Arbor/1/66, the master donor virus for live-attenuated influenza flumist vaccines</article-title><source>Virology</source><year>2008</year><volume>380</volume><fpage>354</fpage><lpage>362</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2008.08.010</pub-id><pub-id pub-id-type="pmid">18804834</pub-id></element-citation></ref><ref id="B108-ijms-18-00020"><label>108.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoffmann</surname><given-names>E.</given-names></name><name><surname>Mahmood</surname><given-names>K.</given-names></name><name><surname>Chen</surname><given-names>Z.</given-names></name><name><surname>Yang</surname><given-names>C.F.</given-names></name><name><surname>Spaete</surname><given-names>J.</given-names></name><name><surname>Greenberg</surname><given-names>H.B.</given-names></name><name><surname>Herlocher</surname><given-names>M.L.</given-names></name><name><surname>Jin</surname><given-names>H.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name></person-group><article-title>Multiple gene segments control the temperature sensitivity and attenuation phenotypes of ca B/Ann Arbor/1/66</article-title><source>J. Virol.</source><year>2005</year><volume>79</volume><fpage>11014</fpage><lpage>11021</lpage><pub-id pub-id-type="doi">10.1128/JVI.79.17.11014-11021.2005</pub-id><pub-id pub-id-type="pmid">16103152</pub-id></element-citation></ref><ref id="B109-ijms-18-00020"><label>109.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>DeBorde</surname><given-names>D.C.</given-names></name><name><surname>Donabedian</surname><given-names>A.M.</given-names></name><name><surname>Herlocher</surname><given-names>M.L.</given-names></name><name><surname>Naeve</surname><given-names>C.W.</given-names></name><name><surname>Maassab</surname><given-names>H.F.</given-names></name></person-group><article-title>Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes</article-title><source>Virology</source><year>1988</year><volume>163</volume><fpage>429</fpage><lpage>443</lpage><pub-id pub-id-type="doi">10.1016/0042-6822(88)90284-X</pub-id><pub-id pub-id-type="pmid">3354202</pub-id></element-citation></ref><ref id="B110-ijms-18-00020"><label>110.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Donabedian</surname><given-names>A.M.</given-names></name><name><surname>DeBorde</surname><given-names>D.C.</given-names></name><name><surname>Maassab</surname><given-names>H.F.</given-names></name></person-group><article-title>Genetics of cold-adapted B/Ann Arbor/1/66 influenza virus reassortants: The acidic polymerase (PA) protein gene confers temperature sensitivity and attenuated virulence</article-title><source>Microb. Pathog.</source><year>1987</year><volume>3</volume><fpage>97</fpage><lpage>108</lpage><pub-id pub-id-type="doi">10.1016/0882-4010(87)90068-4</pub-id><pub-id pub-id-type="pmid">3504545</pub-id></element-citation></ref><ref id="B111-ijms-18-00020"><label>111.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chan</surname><given-names>W.</given-names></name><name><surname>Zhou</surname><given-names>H.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name><name><surname>Jin</surname><given-names>H.</given-names></name></person-group><article-title>The cold adapted and temperature sensitive influenza A/Ann Arbor/6/60 virus, the master donor virus for live-attenuated influenza vaccines, has multiple defects in replication at the restrictive temperature</article-title><source>Virology</source><year>2008</year><volume>380</volume><fpage>304</fpage><lpage>311</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2008.07.027</pub-id><pub-id pub-id-type="pmid">18768193</pub-id></element-citation></ref><ref id="B112-ijms-18-00020"><label>112.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jin</surname><given-names>H.</given-names></name><name><surname>Zhou</surname><given-names>H.</given-names></name><name><surname>Lu</surname><given-names>B.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name></person-group><article-title>Imparting temperature sensitivity and attenuation in ferrets to A/Puerto Rico/8/34 influenza virus by transferring the genetic signature for temperature sensitivity from cold-adapted A/Ann Arbor/6/60</article-title><source>J. Virol.</source><year>2004</year><volume>78</volume><fpage>995</fpage><lpage>998</lpage><pub-id pub-id-type="doi">10.1128/JVI.78.2.995-998.2004</pub-id><pub-id pub-id-type="pmid">14694130</pub-id></element-citation></ref><ref id="B113-ijms-18-00020"><label>113.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jin</surname><given-names>H.</given-names></name><name><surname>Lu</surname><given-names>B.</given-names></name><name><surname>Zhou</surname><given-names>H.</given-names></name><name><surname>Ma</surname><given-names>C.</given-names></name><name><surname>Zhao</surname><given-names>J.</given-names></name><name><surname>Yang</surname><given-names>C.F.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name><name><surname>Greenberg</surname><given-names>H.</given-names></name></person-group><article-title>Multiple amino acid residues confer temperature sensitivity to human influenza virus vaccine strains (flumist) derived from cold-adapted A/Ann Arbor/6/60</article-title><source>Virology</source><year>2003</year><volume>306</volume><fpage>18</fpage><lpage>24</lpage><pub-id pub-id-type="doi">10.1016/S0042-6822(02)00035-1</pub-id><pub-id pub-id-type="pmid">12620793</pub-id></element-citation></ref><ref id="B114-ijms-18-00020"><label>114.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Maassab</surname><given-names>H.F.</given-names></name></person-group><article-title>Adaptation and growth characteristics of influenza virus at 25 °C</article-title><source>Nature</source><year>1967</year><volume>213</volume><fpage>612</fpage><lpage>614</lpage><pub-id pub-id-type="doi">10.1038/213612a0</pub-id><pub-id pub-id-type="pmid">6040602</pub-id></element-citation></ref><ref id="B115-ijms-18-00020"><label>115.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cox</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Dewhurst</surname><given-names>S.</given-names></name></person-group><article-title>Development of a mouse-adapted live-attenuated influenza virus that permits in vivo analysis of enhancements to the safety of live-attenuated influenza virus vaccine</article-title><source>J. Virol.</source><year>2015</year><volume>89</volume><fpage>3421</fpage><lpage>3426</lpage><pub-id pub-id-type="doi">10.1128/JVI.02636-14</pub-id><pub-id pub-id-type="pmid">25552727</pub-id></element-citation></ref><ref id="B116-ijms-18-00020"><label>116.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Greenhawt</surname><given-names>M.J.</given-names></name></person-group><article-title>Influenza vaccination in asthmatic patients</article-title><source>J. Allergy Clin. Immunol.</source><year>2014</year><volume>133</volume><fpage>1233</fpage><lpage>1234</lpage><pub-id pub-id-type="doi">10.1016/j.jaci.2014.02.009</pub-id><pub-id pub-id-type="pmid">24679474</pub-id></element-citation></ref><ref id="B117-ijms-18-00020"><label>117.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Belshe</surname><given-names>R.B.</given-names></name><name><surname>Gruber</surname><given-names>W.C.</given-names></name></person-group><article-title>Safety, efficacy and effectiveness of cold-adapted, live, attenuated, trivalent, intranasal influenza vaccine in adults and children</article-title><source>Philos. Trans. R. Soc. Lond. B Biol. Sci.</source><year>2001</year><volume>356</volume><fpage>1947</fpage><lpage>1951</lpage><pub-id pub-id-type="pmid">11779396</pub-id></element-citation></ref><ref id="B118-ijms-18-00020"><label>118.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ghendon</surname><given-names>Y.Z.</given-names></name><name><surname>Polezhaev</surname><given-names>F.I.</given-names></name><name><surname>Lisovskaya</surname><given-names>K.V.</given-names></name><name><surname>Medvedeva</surname><given-names>T.E.</given-names></name><name><surname>Alexandrova</surname><given-names>G.I.</given-names></name><name><surname>Klimov</surname><given-names>A.I.</given-names></name></person-group><article-title>Recombinant cold-adapted attenuated influenza A vaccines for use in children: Molecular genetic analysis of the cold-adapted donor and recombinants</article-title><source>Infect. Immun.</source><year>1984</year><volume>44</volume><fpage>730</fpage><lpage>733</lpage><pub-id pub-id-type="pmid">6724694</pub-id></element-citation></ref><ref id="B119-ijms-18-00020"><label>119.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ghendon</surname><given-names>Y.Z.</given-names></name></person-group><article-title>Molecular characteristics and biological properties (genetic markers) of candidate strains for preparation of live influenza virus vaccines</article-title><source>Bull. World Health Organ.</source><year>1984</year><volume>62</volume><fpage>493</fpage><lpage>499</lpage><pub-id pub-id-type="pmid">6331910</pub-id></element-citation></ref><ref id="B120-ijms-18-00020"><label>120.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Isakova-Sivak</surname><given-names>I.</given-names></name><name><surname>Chen</surname><given-names>L.M.</given-names></name><name><surname>Matsuoka</surname><given-names>Y.</given-names></name><name><surname>Voeten</surname><given-names>J.T.</given-names></name><name><surname>Kiseleva</surname><given-names>I.</given-names></name><name><surname>Heldens</surname><given-names>J.G.</given-names></name><name><surname>den Bosch</surname><given-names>H.</given-names></name><name><surname>Klimov</surname><given-names>A.</given-names></name><name><surname>Rudenko</surname><given-names>L.</given-names></name><name><surname>Cox</surname><given-names>N.J.</given-names></name><etal></etal></person-group><article-title>Genetic bases of the temperature-sensitive phenotype of a master donor virus used in live-attenuated influenza vaccines: A/Leningrad/134/17/57 (H2N2)</article-title><source>Virology</source><year>2011</year><volume>412</volume><fpage>297</fpage><lpage>305</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2011.01.004</pub-id><pub-id pub-id-type="pmid">21315402</pub-id></element-citation></ref><ref id="B121-ijms-18-00020"><label>121.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kendal</surname><given-names>A.P.</given-names></name></person-group><article-title>Cold-adapted live-attenuated influenza vaccines developed in russia: Can they contribute to meeting the needs for influenza control in other countries?</article-title><source>Eur. J. Epidemiol.</source><year>1997</year><volume>13</volume><fpage>591</fpage><lpage>609</lpage><pub-id pub-id-type="doi">10.1023/A:1007327505862</pub-id><pub-id pub-id-type="pmid">9258574</pub-id></element-citation></ref><ref id="B122-ijms-18-00020"><label>122.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hussain</surname><given-names>A.I.</given-names></name><name><surname>Cordeiro</surname><given-names>M.</given-names></name><name><surname>Sevilla</surname><given-names>E.</given-names></name><name><surname>Liu</surname><given-names>J.</given-names></name></person-group><article-title>Comparison of egg and high yielding MDCK cell-derived live-attenuated influenza virus for commercial production of trivalent influenza vaccine: In vitro cell susceptibility and influenza virus replication kinetics in permissive and semi-permissive cells</article-title><source>Vaccine</source><year>2010</year><volume>28</volume><fpage>3848</fpage><lpage>3855</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2010.03.005</pub-id><pub-id pub-id-type="pmid">20307595</pub-id></element-citation></ref><ref id="B123-ijms-18-00020"><label>123.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>Z.</given-names></name><name><surname>Wang</surname><given-names>W.</given-names></name><name><surname>Zhou</surname><given-names>H.</given-names></name><name><surname>Suguitan</surname><given-names>A.L.</given-names><suffix>Jr.</suffix></name><name><surname>Shambaugh</surname><given-names>C.</given-names></name><name><surname>Kim</surname><given-names>L.</given-names></name><name><surname>Zhao</surname><given-names>J.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name><name><surname>Jin</surname><given-names>H.</given-names></name></person-group><article-title>Generation of live-attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs</article-title><source>J. Virol.</source><year>2010</year><volume>84</volume><fpage>44</fpage><lpage>51</lpage><pub-id pub-id-type="doi">10.1128/JVI.02106-09</pub-id><pub-id pub-id-type="pmid">19864389</pub-id></element-citation></ref><ref id="B124-ijms-18-00020"><label>124.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pearson</surname><given-names>H.E.</given-names></name></person-group><article-title>Distribution of influenza virus type a in infected eggs and the survival of virus under certain conditions of storage</article-title><source>J. Bacteriol.</source><year>1944</year><volume>48</volume><fpage>369</fpage><lpage>378</lpage><pub-id pub-id-type="pmid">16560843</pub-id></element-citation></ref><ref id="B125-ijms-18-00020"><label>125.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Voyles</surname><given-names>C.</given-names></name></person-group><article-title>A flu vaccine shortage can be devastating for LTC residents</article-title><source>RN</source><year>2005</year><volume>68</volume><fpage>10</fpage><pub-id pub-id-type="pmid">15736762</pub-id></element-citation></ref><ref id="B126-ijms-18-00020"><label>126.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Stulc</surname><given-names>J.P.</given-names></name></person-group><article-title>The politics of the flu vaccine shortage</article-title><source>J. Ky. Med. Assoc.</source><year>2004</year><volume>102</volume><fpage>563</fpage><lpage>564</lpage><pub-id pub-id-type="pmid">15636192</pub-id></element-citation></ref><ref id="B127-ijms-18-00020"><label>127.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Genzel</surname><given-names>Y.</given-names></name><name><surname>Reichl</surname><given-names>U.</given-names></name></person-group><article-title>Continuous cell lines as a production system for influenza vaccines</article-title><source>Expert Rev. Vaccines</source><year>2009</year><volume>8</volume><fpage>1681</fpage><lpage>1692</lpage><pub-id pub-id-type="doi">10.1586/erv.09.128</pub-id><pub-id pub-id-type="pmid">19943763</pub-id></element-citation></ref><ref id="B128-ijms-18-00020"><label>128.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Le Ru</surname><given-names>A.</given-names></name><name><surname>Jacob</surname><given-names>D.</given-names></name><name><surname>Transfiguracion</surname><given-names>J.</given-names></name><name><surname>Ansorge</surname><given-names>S.</given-names></name><name><surname>Henry</surname><given-names>O.</given-names></name><name><surname>Kamen</surname><given-names>A.A.</given-names></name></person-group><article-title>Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing</article-title><source>Vaccine</source><year>2010</year><volume>28</volume><fpage>3661</fpage><lpage>3671</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2010.03.029</pub-id><pub-id pub-id-type="pmid">20347632</pub-id></element-citation></ref><ref id="B129-ijms-18-00020"><label>129.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Merten</surname><given-names>O.W.</given-names></name><name><surname>Manuguerra</surname><given-names>J.C.</given-names></name><name><surname>Hannoun</surname><given-names>C.</given-names></name><name><surname>van der Werf</surname><given-names>S.</given-names></name></person-group><article-title>Production of influenza virus in serum-free mammalian cell cultures</article-title><source>Dev. Biol. Stand.</source><year>1999</year><volume>98</volume><fpage>23</fpage><lpage>37</lpage><pub-id pub-id-type="pmid">10494957</pub-id></element-citation></ref><ref id="B130-ijms-18-00020"><label>130.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Merten</surname><given-names>O.W.</given-names></name><name><surname>Hannoun</surname><given-names>C.</given-names></name><name><surname>Manuguerra</surname><given-names>J.C.</given-names></name><name><surname>Ventre</surname><given-names>F.</given-names></name><name><surname>Petres</surname><given-names>S.</given-names></name></person-group><article-title>Production of influenza virus in cell cultures for vaccine preparation</article-title><source>Adv. Exp. Med. Biol.</source><year>1996</year><volume>397</volume><fpage>141</fpage><lpage>151</lpage><pub-id pub-id-type="pmid">8718593</pub-id></element-citation></ref><ref id="B131-ijms-18-00020"><label>131.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Del Giudice</surname><given-names>G.</given-names></name><name><surname>Rappuoli</surname><given-names>R.</given-names></name></person-group><article-title>Inactivated and adjuvanted influenza vaccines</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2015</year><volume>386</volume><fpage>151</fpage><lpage>180</lpage><pub-id pub-id-type="pmid">25038938</pub-id></element-citation></ref><ref id="B132-ijms-18-00020"><label>132.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wu</surname><given-names>J.</given-names></name><name><surname>Liu</surname><given-names>S.Z.</given-names></name><name><surname>Dong</surname><given-names>S.S.</given-names></name><name><surname>Dong</surname><given-names>X.P.</given-names></name><name><surname>Zhang</surname><given-names>W.L.</given-names></name><name><surname>Lu</surname><given-names>M.</given-names></name><name><surname>Li</surname><given-names>C.G.</given-names></name><name><surname>Zhou</surname><given-names>J.C.</given-names></name><name><surname>Fang</surname><given-names>H.H.</given-names></name><name><surname>Liu</surname><given-names>Y.</given-names></name><etal></etal></person-group><article-title>Safety and immunogenicity of adjuvanted inactivated split-virion and whole-virion influenza A (H5N1) vaccines in children: A phase I-II randomized trial</article-title><source>Vaccine</source><year>2010</year><volume>28</volume><fpage>6221</fpage><lpage>6227</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2010.07.008</pub-id><pub-id pub-id-type="pmid">20638454</pub-id></element-citation></ref><ref id="B133-ijms-18-00020"><label>133.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Black</surname><given-names>S.</given-names></name></person-group><article-title>Safety and effectiveness of MF-59 adjuvanted influenza vaccines in children and adults</article-title><source>Vaccine</source><year>2015</year><volume>33</volume><fpage>B3</fpage><lpage>B5</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2014.11.062</pub-id><pub-id pub-id-type="pmid">26022564</pub-id></element-citation></ref><ref id="B134-ijms-18-00020"><label>134.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>W.H.</given-names></name><name><surname>Jackson</surname><given-names>L.A.</given-names></name><name><surname>Edwards</surname><given-names>K.M.</given-names></name><name><surname>Keitel</surname><given-names>W.A.</given-names></name><name><surname>Hill</surname><given-names>H.</given-names></name><name><surname>Noah</surname><given-names>D.L.</given-names></name><name><surname>Creech</surname><given-names>C.B.</given-names></name><name><surname>Patel</surname><given-names>S.M.</given-names></name><name><surname>Mangal</surname><given-names>B.</given-names></name><name><surname>Kotloff</surname><given-names>K.L.</given-names></name></person-group><article-title>Persistence of antibody to influenza A/H5N1 vaccine virus: Impact of AS03 adjuvant</article-title><source>Clin. Vaccine. Immunol.</source><year>2016</year><volume>23</volume><fpage>73</fpage><lpage>77</lpage><pub-id pub-id-type="doi">10.1128/CVI.00475-15</pub-id><pub-id pub-id-type="pmid">26561390</pub-id></element-citation></ref><ref id="B135-ijms-18-00020"><label>135.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Stobart</surname><given-names>C.C.</given-names></name><name><surname>Moore</surname><given-names>M.L.</given-names></name></person-group><article-title>RNA virus reverse genetics and vaccine design</article-title><source>Viruses</source><year>2014</year><volume>6</volume><fpage>2531</fpage><lpage>2550</lpage><pub-id pub-id-type="doi">10.3390/v6072531</pub-id><pub-id pub-id-type="pmid">24967693</pub-id></element-citation></ref><ref id="B136-ijms-18-00020"><label>136.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Almazan</surname><given-names>F.</given-names></name><name><surname>Marquez-Jurado</surname><given-names>S.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Enjuanes</surname><given-names>L.</given-names></name></person-group><article-title>Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes</article-title><source>Methods Mol. Biol.</source><year>2015</year><volume>1282</volume><fpage>135</fpage><lpage>152</lpage><pub-id pub-id-type="pmid">25720478</pub-id></element-citation></ref><ref id="B137-ijms-18-00020"><label>137.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Almazan</surname><given-names>F.</given-names></name><name><surname>Dediego</surname><given-names>M.L.</given-names></name><name><surname>Galan</surname><given-names>C.</given-names></name><name><surname>Escors</surname><given-names>D.</given-names></name><name><surname>Alvarez</surname><given-names>E.</given-names></name><name><surname>Ortego</surname><given-names>J.</given-names></name><name><surname>Sola</surname><given-names>I.</given-names></name><name><surname>Zuniga</surname><given-names>S.</given-names></name><name><surname>Alonso</surname><given-names>S.</given-names></name><name><surname>Moreno</surname><given-names>J.L.</given-names></name><etal></etal></person-group><article-title>Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis</article-title><source>J. Virol.</source><year>2006</year><volume>80</volume><fpage>10900</fpage><lpage>10906</lpage><pub-id pub-id-type="doi">10.1128/JVI.00385-06</pub-id><pub-id pub-id-type="pmid">16928748</pub-id></element-citation></ref><ref id="B138-ijms-18-00020"><label>138.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Racaniello</surname><given-names>V.R.</given-names></name><name><surname>Baltimore</surname><given-names>D.</given-names></name></person-group><article-title>Cloned poliovirus complementary DNA is infectious in mammalian cells</article-title><source>Science</source><year>1981</year><volume>214</volume><fpage>916</fpage><lpage>919</lpage><pub-id pub-id-type="doi">10.1126/science.6272391</pub-id><pub-id pub-id-type="pmid">6272391</pub-id></element-citation></ref><ref id="B139-ijms-18-00020"><label>139.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Generation of recombinant influenza virus from plasmid DNA</article-title><source>J. Vis. Exp.</source><year>2010</year><volume>42</volume><elocation-id>e2057</elocation-id><pub-id pub-id-type="doi">10.3791/2057</pub-id><pub-id pub-id-type="pmid">20729804</pub-id></element-citation></ref><ref id="B140-ijms-18-00020"><label>140.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Engelhardt</surname><given-names>O.G.</given-names></name></person-group><article-title>Many ways to make an influenza virus—Review of influenza virus reverse genetics methods</article-title><source>Influenza Other Respir. Viruses</source><year>2013</year><volume>7</volume><fpage>249</fpage><lpage>256</lpage><pub-id pub-id-type="doi">10.1111/j.1750-2659.2012.00392.x</pub-id><pub-id pub-id-type="pmid">22712782</pub-id></element-citation></ref><ref id="B141-ijms-18-00020"><label>141.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Ortiz-Riano</surname><given-names>E.</given-names></name><name><surname>Dewhurst</surname><given-names>S.</given-names></name><name><surname>Topham</surname><given-names>D.J.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Influenza a virus attenuation by codon deoptimization of the NS gene for vaccine development</article-title><source>J. Virol.</source><year>2014</year><volume>88</volume><fpage>10525</fpage><lpage>10540</lpage><pub-id pub-id-type="doi">10.1128/JVI.01565-14</pub-id><pub-id pub-id-type="pmid">24965472</pub-id></element-citation></ref><ref id="B142-ijms-18-00020"><label>142.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Replication-competent influenza A viruses expressing a red fluorescent protein</article-title><source>Virology</source><year>2014</year><volume>476C</volume><fpage>206</fpage><lpage>216</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2014.12.006</pub-id><pub-id pub-id-type="pmid">25553516</pub-id></element-citation></ref><ref id="B143-ijms-18-00020"><label>143.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Cadagan</surname><given-names>R.</given-names></name><name><surname>Steel</surname><given-names>J.</given-names></name><name><surname>Basler</surname><given-names>C.F.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name><name><surname>Moran</surname><given-names>T.M.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Hemagglutinin-pseudotyped green fluorescent protein-expressing influenza viruses for the detection of influenza virus neutralizing antibodies</article-title><source>J. Virol.</source><year>2010</year><volume>84</volume><fpage>2157</fpage><lpage>2163</lpage><pub-id pub-id-type="doi">10.1128/JVI.01433-09</pub-id><pub-id pub-id-type="pmid">19939917</pub-id></element-citation></ref><ref id="B144-ijms-18-00020"><label>144.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Domm</surname><given-names>W.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Development and applications of single-cycle infectious influenza A virus (sciIAV)</article-title><source>Virus Res.</source><year>2015</year><volume>216</volume><fpage>26</fpage><lpage>40</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2015.07.013</pub-id><pub-id pub-id-type="pmid">26220478</pub-id></element-citation></ref><ref id="B145-ijms-18-00020"><label>145.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Breen</surname><given-names>M.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Replication-competent influenza A viruses expressing reporter genes</article-title><source>Viruses</source><year>2016</year><volume>8</volume><elocation-id>179</elocation-id><pub-id pub-id-type="doi">10.3390/v8070179</pub-id><pub-id pub-id-type="pmid">27347991</pub-id></element-citation></ref><ref id="B146-ijms-18-00020"><label>146.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Rodriguez-Sanchez</surname><given-names>I.</given-names></name><name><surname>Monte</surname><given-names>K.</given-names></name><name><surname>Lenschow</surname><given-names>D.J.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Replication-competent fluorescent-expressing influenza B virus</article-title><source>Virus Res.</source><year>2015</year><volume>213</volume><fpage>69</fpage><lpage>81</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2015.11.014</pub-id><pub-id pub-id-type="pmid">26590325</pub-id></element-citation></ref><ref id="B147-ijms-18-00020"><label>147.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Breen</surname><given-names>M.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Replication-competent influenza A and B viruses expressing a fluorescent dynamic timer protein for in vitro and in vivo studies</article-title><source>PLoS ONE</source><year>2016</year><volume>11</volume><elocation-id>e0147723</elocation-id><pub-id pub-id-type="doi">10.1371/journal.pone.0147723</pub-id><pub-id pub-id-type="pmid">26809059</pub-id></element-citation></ref><ref id="B148-ijms-18-00020"><label>148.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zobel</surname><given-names>A.</given-names></name><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Hobom</surname><given-names>G.</given-names></name></person-group><article-title>RNA polymerase I catalysed transcription of insert viral cDNA</article-title><source>Nucleic Acids Res.</source><year>1993</year><volume>21</volume><fpage>3607</fpage><lpage>3614</lpage><pub-id pub-id-type="doi">10.1093/nar/21.16.3607</pub-id><pub-id pub-id-type="pmid">8367275</pub-id></element-citation></ref><ref id="B149-ijms-18-00020"><label>149.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Zobel</surname><given-names>A.</given-names></name><name><surname>Hobom</surname><given-names>G.</given-names></name></person-group><article-title>RNA polymerase I-mediated expression of influenza viral RNA molecules</article-title><source>Virology</source><year>1994</year><volume>202</volume><fpage>477</fpage><lpage>479</lpage><pub-id pub-id-type="doi">10.1006/viro.1994.1365</pub-id><pub-id pub-id-type="pmid">8009859</pub-id></element-citation></ref><ref id="B150-ijms-18-00020"><label>150.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoffmann</surname><given-names>E.</given-names></name><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name><name><surname>Hobom</surname><given-names>G.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>A DNA transfection system for generation of influenza A virus from eight plasmids</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2000</year><volume>97</volume><fpage>6108</fpage><lpage>6113</lpage><pub-id pub-id-type="doi">10.1073/pnas.100133697</pub-id><pub-id pub-id-type="pmid">10801978</pub-id></element-citation></ref><ref id="B151-ijms-18-00020"><label>151.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoffmann</surname><given-names>E.</given-names></name><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Hobom</surname><given-names>G.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>“Ambisense” approach for the generation of influenza A virus: vRNA and mRNA synthesis from one template</article-title><source>Virology</source><year>2000</year><volume>267</volume><fpage>310</fpage><lpage>317</lpage><pub-id pub-id-type="doi">10.1006/viro.1999.0140</pub-id><pub-id pub-id-type="pmid">10662626</pub-id></element-citation></ref><ref id="B152-ijms-18-00020"><label>152.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cheng</surname><given-names>B.Y.</given-names></name><name><surname>Ortiz-Riano</surname><given-names>E.</given-names></name><name><surname>de la Torre</surname><given-names>J.C.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Generation of recombinant arenavirus for vaccine development in fda-approved vero cells</article-title><source>J. Vis. Exp.</source><year>2013</year><volume>78</volume><elocation-id>e50662</elocation-id><pub-id pub-id-type="doi">10.3791/50662</pub-id><pub-id pub-id-type="pmid">23928556</pub-id></element-citation></ref><ref id="B153-ijms-18-00020"><label>153.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ortiz-Riano</surname><given-names>E.</given-names></name><name><surname>Cheng</surname><given-names>B.Y.</given-names></name><name><surname>Carlos de la Torre</surname><given-names>J.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Arenavirus reverse genetics for vaccine development</article-title><source>J. Gen. Virol.</source><year>2013</year><volume>94</volume><fpage>1175</fpage><lpage>1188</lpage><pub-id pub-id-type="doi">10.1099/vir.0.051102-0</pub-id><pub-id pub-id-type="pmid">23364194</pub-id></element-citation></ref><ref id="B154-ijms-18-00020"><label>154.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Neumann</surname><given-names>G.</given-names></name><name><surname>Watanabe</surname><given-names>T.</given-names></name><name><surname>Ito</surname><given-names>H.</given-names></name><name><surname>Watanabe</surname><given-names>S.</given-names></name><name><surname>Goto</surname><given-names>H.</given-names></name><name><surname>Gao</surname><given-names>P.</given-names></name><name><surname>Hughes</surname><given-names>M.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Donis</surname><given-names>R.</given-names></name><name><surname>Hoffmann</surname><given-names>E.</given-names></name><etal></etal></person-group><article-title>Generation of influenza A viruses entirely from cloned cDNAs</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>1999</year><volume>96</volume><fpage>9345</fpage><lpage>9350</lpage><pub-id pub-id-type="doi">10.1073/pnas.96.16.9345</pub-id><pub-id pub-id-type="pmid">10430945</pub-id></element-citation></ref><ref id="B155-ijms-18-00020"><label>155.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>Z.</given-names></name><name><surname>Duke</surname><given-names>G.M.</given-names></name></person-group><article-title>Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells</article-title><source>Virol. J.</source><year>2007</year><volume>4</volume><fpage>102</fpage><pub-id pub-id-type="doi">10.1186/1743-422X-4-102</pub-id><pub-id pub-id-type="pmid">17956624</pub-id></element-citation></ref><ref id="B156-ijms-18-00020"><label>156.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Massin</surname><given-names>P.</given-names></name><name><surname>Rodrigues</surname><given-names>P.</given-names></name><name><surname>Marasescu</surname><given-names>M.</given-names></name><name><surname>van der Werf</surname><given-names>S.</given-names></name><name><surname>Naffakh</surname><given-names>N.</given-names></name></person-group><article-title>Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells</article-title><source>J. Virol.</source><year>2005</year><volume>79</volume><fpage>13811</fpage><lpage>13816</lpage><pub-id pub-id-type="doi">10.1128/JVI.79.21.13811-13816.2005</pub-id><pub-id pub-id-type="pmid">16227302</pub-id></element-citation></ref><ref id="B157-ijms-18-00020"><label>157.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>X.</given-names></name><name><surname>Curtiss</surname><given-names>R.</given-names><suffix>3rd</suffix></name></person-group><article-title>Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid</article-title><source>Virol. J.</source><year>2015</year><volume>12</volume><fpage>95</fpage><pub-id pub-id-type="doi">10.1186/s12985-015-0321-5</pub-id><pub-id pub-id-type="pmid">26093583</pub-id></element-citation></ref><ref id="B158-ijms-18-00020"><label>158.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lu</surname><given-names>G.</given-names></name><name><surname>He</surname><given-names>D.</given-names></name><name><surname>Wang</surname><given-names>Z.</given-names></name><name><surname>Ou</surname><given-names>S.</given-names></name><name><surname>Yuan</surname><given-names>R.</given-names></name><name><surname>Li</surname><given-names>S.</given-names></name></person-group><article-title>Cloning the horse RNA polymerase I promoter and its application to studying influenza virus polymerase activity</article-title><source>Viruses</source><year>2016</year><volume>8</volume><elocation-id>119</elocation-id><pub-id pub-id-type="doi">10.3390/v8060119</pub-id><pub-id pub-id-type="pmid">27258298</pub-id></element-citation></ref><ref id="B159-ijms-18-00020"><label>159.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fodor</surname><given-names>E.</given-names></name><name><surname>Devenish</surname><given-names>L.</given-names></name><name><surname>Engelhardt</surname><given-names>O.G.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name><name><surname>Brownlee</surname><given-names>G.G.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Rescue of influenza A virus from recombinant DNA</article-title><source>J. Virol.</source><year>1999</year><volume>73</volume><fpage>9679</fpage><lpage>9682</lpage><pub-id pub-id-type="pmid">10516084</pub-id></element-citation></ref><ref id="B160-ijms-18-00020"><label>160.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhou</surname><given-names>B.</given-names></name><name><surname>Lin</surname><given-names>X.</given-names></name><name><surname>Wang</surname><given-names>W.</given-names></name><name><surname>Halpin</surname><given-names>R.A.</given-names></name><name><surname>Bera</surname><given-names>J.</given-names></name><name><surname>Stockwell</surname><given-names>T.B.</given-names></name><name><surname>Barr</surname><given-names>I.G.</given-names></name><name><surname>Wentworth</surname><given-names>D.E.</given-names></name></person-group><article-title>Universal influenza B virus genomic amplification facilitates sequencing, diagnostics, and reverse genetics</article-title><source>J. Clin. Microbiol.</source><year>2014</year><volume>52</volume><fpage>1330</fpage><lpage>1337</lpage><pub-id pub-id-type="doi">10.1128/JCM.03265-13</pub-id><pub-id pub-id-type="pmid">24501036</pub-id></element-citation></ref><ref id="B161-ijms-18-00020"><label>161.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jackson</surname><given-names>D.</given-names></name><name><surname>Cadman</surname><given-names>A.</given-names></name><name><surname>Zurcher</surname><given-names>T.</given-names></name><name><surname>Barclay</surname><given-names>W.S.</given-names></name></person-group><article-title>A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA</article-title><source>J. Virol.</source><year>2002</year><volume>76</volume><fpage>11744</fpage><lpage>11747</lpage><pub-id pub-id-type="doi">10.1128/JVI.76.22.11744-11747.2002</pub-id><pub-id pub-id-type="pmid">12388735</pub-id></element-citation></ref><ref id="B162-ijms-18-00020"><label>162.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoffmann</surname><given-names>E.</given-names></name><name><surname>Mahmood</surname><given-names>K.</given-names></name><name><surname>Yang</surname><given-names>C.F.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name><name><surname>Greenberg</surname><given-names>H.B.</given-names></name><name><surname>Kemble</surname><given-names>G.</given-names></name></person-group><article-title>Rescue of influenza B virus from eight plasmids</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2002</year><volume>99</volume><fpage>11411</fpage><lpage>11416</lpage><pub-id pub-id-type="doi">10.1073/pnas.172393399</pub-id><pub-id pub-id-type="pmid">12172012</pub-id></element-citation></ref><ref id="B163-ijms-18-00020"><label>163.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ozawa</surname><given-names>M.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>Taming influenza viruses</article-title><source>Virus Res.</source><year>2011</year><volume>162</volume><fpage>8</fpage><lpage>11</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2011.09.035</pub-id><pub-id pub-id-type="pmid">21968297</pub-id></element-citation></ref><ref id="B164-ijms-18-00020"><label>164.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marsh</surname><given-names>G.A.</given-names></name><name><surname>Hatami</surname><given-names>R.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Specific residues of the influenza A virus hemagglutinin viral RNA are important for efficient packaging into budding virions</article-title><source>J. Virol.</source><year>2007</year><volume>81</volume><fpage>9727</fpage><lpage>9736</lpage><pub-id pub-id-type="doi">10.1128/JVI.01144-07</pub-id><pub-id pub-id-type="pmid">17634232</pub-id></element-citation></ref><ref id="B165-ijms-18-00020"><label>165.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Isel</surname><given-names>C.</given-names></name><name><surname>Munier</surname><given-names>S.</given-names></name><name><surname>Naffakh</surname><given-names>N.</given-names></name></person-group><article-title>Experimental approaches to study genome packaging of influenza A viruses</article-title><source>Viruses</source><year>2016</year><volume>8</volume><elocation-id>218</elocation-id><pub-id pub-id-type="doi">10.3390/v8080218</pub-id><pub-id pub-id-type="pmid">27517951</pub-id></element-citation></ref><ref id="B166-ijms-18-00020"><label>166.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Schickli</surname><given-names>J.H.</given-names></name><name><surname>Flandorfer</surname><given-names>A.</given-names></name><name><surname>Nakaya</surname><given-names>T.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Plasmid-only rescue of influenza A virus vaccine candidates</article-title><source>Philos. Trans. R. Soc. Lond. B Biol. Sci.</source><year>2001</year><volume>356</volume><fpage>1965</fpage><lpage>1973</lpage><pub-id pub-id-type="pmid">11779399</pub-id></element-citation></ref><ref id="B167-ijms-18-00020"><label>167.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Steel</surname><given-names>J.</given-names></name><name><surname>Lowen</surname><given-names>A.C.</given-names></name><name><surname>Pena</surname><given-names>L.</given-names></name><name><surname>Angel</surname><given-names>M.</given-names></name><name><surname>Solorzano</surname><given-names>A.</given-names></name><name><surname>Albrecht</surname><given-names>R.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza</article-title><source>J. Virol.</source><year>2009</year><volume>83</volume><fpage>1742</fpage><lpage>1753</lpage><pub-id pub-id-type="doi">10.1128/JVI.01920-08</pub-id><pub-id pub-id-type="pmid">19073731</pub-id></element-citation></ref><ref id="B168-ijms-18-00020"><label>168.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Richt</surname><given-names>J.A.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Attenuated influenza virus vaccines with modified NS1 proteins</article-title><source>Curr. Top. Microbiol. Immunol.</source><year>2009</year><volume>333</volume><fpage>177</fpage><lpage>195</lpage><pub-id pub-id-type="pmid">19768406</pub-id></element-citation></ref><ref id="B169-ijms-18-00020"><label>169.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Vincent</surname><given-names>A.L.</given-names></name><name><surname>Ma</surname><given-names>W.</given-names></name><name><surname>Lager</surname><given-names>K.M.</given-names></name><name><surname>Janke</surname><given-names>B.H.</given-names></name><name><surname>Webby</surname><given-names>R.J.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Richt</surname><given-names>J.A.</given-names></name></person-group><article-title>Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine</article-title><source>Vaccine</source><year>2007</year><volume>25</volume><fpage>7999</fpage><lpage>8009</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2007.09.019</pub-id><pub-id pub-id-type="pmid">17933442</pub-id></element-citation></ref><ref id="B170-ijms-18-00020"><label>170.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Quinlivan</surname><given-names>M.</given-names></name><name><surname>Zamarin</surname><given-names>D.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Cullinane</surname><given-names>A.</given-names></name><name><surname>Chambers</surname><given-names>T.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Attenuation of equine influenza viruses through truncations of the NS1 protein</article-title><source>J. Virol.</source><year>2005</year><volume>79</volume><fpage>8431</fpage><lpage>8439</lpage><pub-id pub-id-type="doi">10.1128/JVI.79.13.8431-8439.2005</pub-id><pub-id pub-id-type="pmid">15956587</pub-id></element-citation></ref><ref id="B171-ijms-18-00020"><label>171.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Falcon</surname><given-names>A.M.</given-names></name><name><surname>Fernandez-Sesma</surname><given-names>A.</given-names></name><name><surname>Nakaya</surname><given-names>Y.</given-names></name><name><surname>Moran</surname><given-names>T.M.</given-names></name><name><surname>Ortin</surname><given-names>J.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>Attenuation and immunogenicity in mice of temperature-sensitive influenza viruses expressing truncated NS1 proteins</article-title><source>J. Gen. Virol.</source><year>2005</year><volume>86</volume><fpage>2817</fpage><lpage>2821</lpage><pub-id pub-id-type="doi">10.1099/vir.0.80991-0</pub-id><pub-id pub-id-type="pmid">16186237</pub-id></element-citation></ref><ref id="B172-ijms-18-00020"><label>172.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ferko</surname><given-names>B.</given-names></name><name><surname>Stasakova</surname><given-names>J.</given-names></name><name><surname>Romanova</surname><given-names>J.</given-names></name><name><surname>Kittel</surname><given-names>C.</given-names></name><name><surname>Sereinig</surname><given-names>S.</given-names></name><name><surname>Katinger</surname><given-names>H.</given-names></name><name><surname>Egorov</surname><given-names>A.</given-names></name></person-group><article-title>Immunogenicity and protection efficacy of replication-deficient influenza A viruses with altered NS1 genes</article-title><source>J. Virol.</source><year>2004</year><volume>78</volume><fpage>13037</fpage><lpage>13045</lpage><pub-id pub-id-type="doi">10.1128/JVI.78.23.13037-13045.2004</pub-id><pub-id pub-id-type="pmid">15542655</pub-id></element-citation></ref><ref id="B173-ijms-18-00020"><label>173.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Richt</surname><given-names>J.A.</given-names></name><name><surname>Lekcharoensuk</surname><given-names>P.</given-names></name><name><surname>Lager</surname><given-names>K.M.</given-names></name><name><surname>Vincent</surname><given-names>A.L.</given-names></name><name><surname>Loiacono</surname><given-names>C.M.</given-names></name><name><surname>Janke</surname><given-names>B.H.</given-names></name><name><surname>Wu</surname><given-names>W.H.</given-names></name><name><surname>Yoon</surname><given-names>K.J.</given-names></name><name><surname>Webby</surname><given-names>R.J.</given-names></name><name><surname>Solorzano</surname><given-names>A.</given-names></name><etal></etal></person-group><article-title>Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine</article-title><source>J. Virol.</source><year>2006</year><volume>80</volume><fpage>11009</fpage><lpage>11018</lpage><pub-id pub-id-type="doi">10.1128/JVI.00787-06</pub-id><pub-id pub-id-type="pmid">16943300</pub-id></element-citation></ref><ref id="B174-ijms-18-00020"><label>174.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Solorzano</surname><given-names>A.</given-names></name><name><surname>Webby</surname><given-names>R.J.</given-names></name><name><surname>Lager</surname><given-names>K.M.</given-names></name><name><surname>Janke</surname><given-names>B.H.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Richt</surname><given-names>J.A.</given-names></name></person-group><article-title>Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs</article-title><source>J. Virol.</source><year>2005</year><volume>79</volume><fpage>7535</fpage><lpage>7543</lpage><pub-id pub-id-type="doi">10.1128/JVI.79.12.7535-7543.2005</pub-id><pub-id pub-id-type="pmid">15919908</pub-id></element-citation></ref><ref id="B175-ijms-18-00020"><label>175.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>L.</given-names></name><name><surname>Suarez</surname><given-names>D.L.</given-names></name><name><surname>Pantin-Jackwood</surname><given-names>M.</given-names></name><name><surname>Mibayashi</surname><given-names>M.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Saif</surname><given-names>Y.M.</given-names></name><name><surname>Lee</surname><given-names>C.W.</given-names></name></person-group><article-title>Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry</article-title><source>Vaccine</source><year>2008</year><volume>26</volume><fpage>3580</fpage><lpage>3586</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2008.05.001</pub-id><pub-id pub-id-type="pmid">18539366</pub-id></element-citation></ref><ref id="B176-ijms-18-00020"><label>176.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Choi</surname><given-names>E.H.</given-names></name><name><surname>Song</surname><given-names>M.S.</given-names></name><name><surname>Park</surname><given-names>S.J.</given-names></name><name><surname>Pascua</surname><given-names>P.N.</given-names></name><name><surname>Baek</surname><given-names>Y.H.</given-names></name><name><surname>Kwon</surname><given-names>H.I.</given-names></name><name><surname>Kim</surname><given-names>E.H.</given-names></name><name><surname>Kim</surname><given-names>S.</given-names></name><name><surname>Jang</surname><given-names>H.K.</given-names></name><name><surname>Poo</surname><given-names>H.</given-names></name><etal></etal></person-group><article-title>Development of a dual-protective live-attenuated vaccine against H5N1 and H9N2 avian influenza viruses by modifying the NS1 gene</article-title><source>Arch. Virol.</source><year>2015</year><volume>160</volume><fpage>1729</fpage><lpage>1740</lpage><pub-id pub-id-type="doi">10.1007/s00705-015-2442-y</pub-id><pub-id pub-id-type="pmid">25959557</pub-id></element-citation></ref><ref id="B177-ijms-18-00020"><label>177.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>Huang</surname><given-names>K.</given-names></name><name><surname>Chauche</surname><given-names>C.</given-names></name><name><surname>DeDiego</surname><given-names>M.L.</given-names></name><name><surname>Murcia</surname><given-names>P.R.</given-names></name><name><surname>Parrish</surname><given-names>C.R.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Canine influenza viruses with modified NS1 proteins for the development of live-attenuated vaccines</article-title><source>Virology</source><year>2016</year><volume>500</volume><fpage>1</fpage><lpage>10</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2016.10.008</pub-id><pub-id pub-id-type="pmid">27750071</pub-id></element-citation></ref><ref id="B178-ijms-18-00020"><label>178.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baskin</surname><given-names>C.R.</given-names></name><name><surname>Bielefeldt-Ohmann</surname><given-names>H.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Tumpey</surname><given-names>T.M.</given-names></name><name><surname>van Hoeven</surname><given-names>N.</given-names></name><name><surname>Carter</surname><given-names>V.S.</given-names></name><name><surname>Thomas</surname><given-names>M.J.</given-names></name><name><surname>Proll</surname><given-names>S.</given-names></name><name><surname>Solorzano</surname><given-names>A.</given-names></name><name><surname>Billharz</surname><given-names>R.</given-names></name><etal></etal></person-group><article-title>Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus</article-title><source>J. Virol.</source><year>2007</year><volume>81</volume><fpage>11817</fpage><lpage>11827</lpage><pub-id pub-id-type="doi">10.1128/JVI.00590-07</pub-id><pub-id pub-id-type="pmid">17715226</pub-id></element-citation></ref><ref id="B179-ijms-18-00020"><label>179.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pica</surname><given-names>N.</given-names></name><name><surname>Langlois</surname><given-names>R.A.</given-names></name><name><surname>Krammer</surname><given-names>F.</given-names></name><name><surname>Margine</surname><given-names>I.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>NS1-truncated live-attenuated virus vaccine provides robust protection to aged mice from viral challenge</article-title><source>J. Virol.</source><year>2012</year><volume>86</volume><fpage>10293</fpage><lpage>10301</lpage><pub-id pub-id-type="doi">10.1128/JVI.01131-12</pub-id><pub-id pub-id-type="pmid">22787224</pub-id></element-citation></ref><ref id="B180-ijms-18-00020"><label>180.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hai</surname><given-names>R.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Fraser</surname><given-names>K.A.</given-names></name><name><surname>Ayllon</surname><given-names>J.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Influenza B virus NS1-truncated mutants: Live-attenuated vaccine approach</article-title><source>J. Virol.</source><year>2008</year><volume>82</volume><fpage>10580</fpage><lpage>10590</lpage><pub-id pub-id-type="doi">10.1128/JVI.01213-08</pub-id><pub-id pub-id-type="pmid">18768976</pub-id></element-citation></ref><ref id="B181-ijms-18-00020"><label>181.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Talon</surname><given-names>J.</given-names></name><name><surname>Salvatore</surname><given-names>M.</given-names></name><name><surname>O'Neill</surname><given-names>R.E.</given-names></name><name><surname>Nakaya</surname><given-names>Y.</given-names></name><name><surname>Zheng</surname><given-names>H.</given-names></name><name><surname>Muster</surname><given-names>T.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name><name><surname>Palese</surname><given-names>P.</given-names></name></person-group><article-title>Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2000</year><volume>97</volume><fpage>4309</fpage><lpage>4314</lpage><pub-id pub-id-type="doi">10.1073/pnas.070525997</pub-id><pub-id pub-id-type="pmid">10725408</pub-id></element-citation></ref><ref id="B182-ijms-18-00020"><label>182.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cheng</surname><given-names>B.Y.</given-names></name><name><surname>Ortiz-Riano</surname><given-names>E.</given-names></name><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>de la Torre</surname><given-names>J.C.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Development of live-attenuated arenavirus vaccines based on codon deoptimization</article-title><source>J. Virol.</source><year>2015</year><volume>89</volume><fpage>3523</fpage><lpage>3533</lpage><pub-id pub-id-type="doi">10.1128/JVI.03401-14</pub-id><pub-id pub-id-type="pmid">25589652</pub-id></element-citation></ref><ref id="B183-ijms-18-00020"><label>183.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mueller</surname><given-names>S.</given-names></name><name><surname>Coleman</surname><given-names>J.R.</given-names></name><name><surname>Papamichail</surname><given-names>D.</given-names></name><name><surname>Ward</surname><given-names>C.B.</given-names></name><name><surname>Nimnual</surname><given-names>A.</given-names></name><name><surname>Futcher</surname><given-names>B.</given-names></name><name><surname>Skiena</surname><given-names>S.</given-names></name><name><surname>Wimmer</surname><given-names>E.</given-names></name></person-group><article-title>Live attenuated influenza virus vaccines by computer-aided rational design</article-title><source>Nat. Biotechnol.</source><year>2010</year><volume>28</volume><fpage>723</fpage><lpage>726</lpage><pub-id pub-id-type="doi">10.1038/nbt.1636</pub-id><pub-id pub-id-type="pmid">20543832</pub-id></element-citation></ref><ref id="B184-ijms-18-00020"><label>184.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yang</surname><given-names>C.</given-names></name><name><surname>Skiena</surname><given-names>S.</given-names></name><name><surname>Futcher</surname><given-names>B.</given-names></name><name><surname>Mueller</surname><given-names>S.</given-names></name><name><surname>Wimmer</surname><given-names>E.</given-names></name></person-group><article-title>Deliberate reduction of hemagglutinin and neuraminidase expression of influenza virus leads to an ultraprotective live vaccine in mice</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2013</year><volume>110</volume><fpage>9481</fpage><lpage>9486</lpage><pub-id pub-id-type="doi">10.1073/pnas.1307473110</pub-id><pub-id pub-id-type="pmid">23690603</pub-id></element-citation></ref><ref id="B185-ijms-18-00020"><label>185.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Katsura</surname><given-names>H.</given-names></name><name><surname>Iwatsuki-Horimoto</surname><given-names>K.</given-names></name><name><surname>Fukuyama</surname><given-names>S.</given-names></name><name><surname>Watanabe</surname><given-names>S.</given-names></name><name><surname>Sakabe</surname><given-names>S.</given-names></name><name><surname>Hatta</surname><given-names>Y.</given-names></name><name><surname>Murakami</surname><given-names>S.</given-names></name><name><surname>Shimojima</surname><given-names>M.</given-names></name><name><surname>Horimoto</surname><given-names>T.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A replication-incompetent virus possessing an uncleavable hemagglutinin as an influenza vaccine</article-title><source>Vaccine</source><year>2012</year><volume>30</volume><fpage>6027</fpage><lpage>6033</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2012.07.059</pub-id><pub-id pub-id-type="pmid">22867723</pub-id></element-citation></ref><ref id="B186-ijms-18-00020"><label>186.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Powell</surname><given-names>T.J.</given-names></name><name><surname>Silk</surname><given-names>J.D.</given-names></name><name><surname>Sharps</surname><given-names>J.</given-names></name><name><surname>Fodor</surname><given-names>E.</given-names></name><name><surname>Townsend</surname><given-names>A.R.</given-names></name></person-group><article-title>Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity</article-title><source>J. Virol.</source><year>2012</year><volume>86</volume><fpage>13397</fpage><lpage>13406</lpage><pub-id pub-id-type="doi">10.1128/JVI.01820-12</pub-id><pub-id pub-id-type="pmid">23015719</pub-id></element-citation></ref><ref id="B187-ijms-18-00020"><label>187.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shinya</surname><given-names>K.</given-names></name><name><surname>Fujii</surname><given-names>Y.</given-names></name><name><surname>Ito</surname><given-names>H.</given-names></name><name><surname>Ito</surname><given-names>T.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>Characterization of a neuraminidase-deficient influenza A virus as a potential gene delivery vector and a live vaccine</article-title><source>J. Virol.</source><year>2004</year><volume>78</volume><fpage>3083</fpage><lpage>3088</lpage><pub-id pub-id-type="doi">10.1128/JVI.78.6.3083-3088.2004</pub-id><pub-id pub-id-type="pmid">14990727</pub-id></element-citation></ref><ref id="B188-ijms-18-00020"><label>188.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Uraki</surname><given-names>R.</given-names></name><name><surname>Kiso</surname><given-names>M.</given-names></name><name><surname>Iwatsuki-Horimoto</surname><given-names>K.</given-names></name><name><surname>Fukuyama</surname><given-names>S.</given-names></name><name><surname>Takashita</surname><given-names>E.</given-names></name><name><surname>Ozawa</surname><given-names>M.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A novel bivalent vaccine based on a PB2-knockout influenza virus protects mice from pandemic H1N1 and highly pathogenic H5N1 virus challenges</article-title><source>J. Virol.</source><year>2013</year><volume>87</volume><fpage>7874</fpage><lpage>7881</lpage><pub-id pub-id-type="doi">10.1128/JVI.00076-13</pub-id><pub-id pub-id-type="pmid">23658445</pub-id></element-citation></ref><ref id="B189-ijms-18-00020"><label>189.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Victor</surname><given-names>S.T.</given-names></name><name><surname>Watanabe</surname><given-names>S.</given-names></name><name><surname>Katsura</surname><given-names>H.</given-names></name><name><surname>Ozawa</surname><given-names>M.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A replication-incompetent PB2-knockout influenza A virus vaccine vector</article-title><source>J. Virol.</source><year>2012</year><volume>86</volume><fpage>4123</fpage><lpage>4128</lpage><pub-id pub-id-type="doi">10.1128/JVI.06232-11</pub-id><pub-id pub-id-type="pmid">22301144</pub-id></element-citation></ref><ref id="B190-ijms-18-00020"><label>190.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Guo</surname><given-names>H.</given-names></name><name><surname>Baker</surname><given-names>S.F.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name><name><surname>Topham</surname><given-names>D.J.</given-names></name></person-group><article-title>Induction of CD8 T cell heterologous protection by a single dose of single-cycle infectious influenza virus</article-title><source>J. Virol.</source><year>2014</year><volume>88</volume><fpage>12006</fpage><lpage>12016</lpage><pub-id pub-id-type="doi">10.1128/JVI.01847-14</pub-id><pub-id pub-id-type="pmid">25100831</pub-id></element-citation></ref><ref id="B191-ijms-18-00020"><label>191.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Masic</surname><given-names>A.</given-names></name><name><surname>Pyo</surname><given-names>H.M.</given-names></name><name><surname>Babiuk</surname><given-names>S.</given-names></name><name><surname>Zhou</surname><given-names>Y.</given-names></name></person-group><article-title>An eight-segment swine influenza virus harboring H1 and H3 hemagglutinins is attenuated and protective against H1N1 and H3N2 subtypes in pigs</article-title><source>J. Virol.</source><year>2013</year><volume>87</volume><fpage>10114</fpage><lpage>10125</lpage><pub-id pub-id-type="doi">10.1128/JVI.01348-13</pub-id><pub-id pub-id-type="pmid">23843633</pub-id></element-citation></ref><ref id="B192-ijms-18-00020"><label>192.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fonseca</surname><given-names>W.</given-names></name><name><surname>Ozawa</surname><given-names>M.</given-names></name><name><surname>Hatta</surname><given-names>M.</given-names></name><name><surname>Orozco</surname><given-names>E.</given-names></name><name><surname>Martinez</surname><given-names>M.B.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A recombinant influenza virus vaccine expressing the f protein of respiratory syncytial virus</article-title><source>Arch. Virol.</source><year>2014</year><volume>159</volume><fpage>1067</fpage><lpage>1077</lpage><pub-id pub-id-type="doi">10.1007/s00705-013-1932-z</pub-id><pub-id pub-id-type="pmid">24292020</pub-id></element-citation></ref><ref id="B193-ijms-18-00020"><label>193.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kobayashi</surname><given-names>H.</given-names></name><name><surname>Iwatsuki-Horimoto</surname><given-names>K.</given-names></name><name><surname>Kiso</surname><given-names>M.</given-names></name><name><surname>Uraki</surname><given-names>R.</given-names></name><name><surname>Ichiko</surname><given-names>Y.</given-names></name><name><surname>Takimoto</surname><given-names>T.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A replication-incompetent influenza virus bearing the hn glycoprotein of human parainfluenza virus as a bivalent vaccine</article-title><source>Vaccine</source><year>2013</year><volume>31</volume><fpage>6239</fpage><lpage>6246</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2013.10.029</pub-id><pub-id pub-id-type="pmid">24144478</pub-id></element-citation></ref><ref id="B194-ijms-18-00020"><label>194.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Katsura</surname><given-names>H.</given-names></name><name><surname>Piao</surname><given-names>Z.</given-names></name><name><surname>Iwatsuki-Horimoto</surname><given-names>K.</given-names></name><name><surname>Akeda</surname><given-names>Y.</given-names></name><name><surname>Watanabe</surname><given-names>S.</given-names></name><name><surname>Horimoto</surname><given-names>T.</given-names></name><name><surname>Oishi</surname><given-names>K.</given-names></name><name><surname>Kawaoka</surname><given-names>Y.</given-names></name></person-group><article-title>A bivalent vaccine based on a replication-incompetent influenza virus protects against streptococcus pneumoniae and influenza virus infection</article-title><source>J. Virol.</source><year>2014</year><volume>88</volume><fpage>13410</fpage><lpage>13417</lpage><pub-id pub-id-type="doi">10.1128/JVI.01205-14</pub-id><pub-id pub-id-type="pmid">25210171</pub-id></element-citation></ref><ref id="B195-ijms-18-00020"><label>195.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pena</surname><given-names>L.</given-names></name><name><surname>Sutton</surname><given-names>T.</given-names></name><name><surname>Chockalingam</surname><given-names>A.</given-names></name><name><surname>Kumar</surname><given-names>S.</given-names></name><name><surname>Angel</surname><given-names>M.</given-names></name><name><surname>Shao</surname><given-names>H.</given-names></name><name><surname>Chen</surname><given-names>H.</given-names></name><name><surname>Li</surname><given-names>W.</given-names></name><name><surname>Perez</surname><given-names>D.R.</given-names></name></person-group><article-title>Influenza viruses with rearranged genomes as live-attenuated vaccines</article-title><source>J. Virol.</source><year>2013</year><volume>87</volume><fpage>5118</fpage><lpage>5127</lpage><pub-id pub-id-type="doi">10.1128/JVI.02490-12</pub-id><pub-id pub-id-type="pmid">23449800</pub-id></element-citation></ref><ref id="B196-ijms-18-00020"><label>196.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nogales</surname><given-names>A.</given-names></name><name><surname>DeDiego</surname><given-names>M.L.</given-names></name><name><surname>Topham</surname><given-names>D.J.</given-names></name><name><surname>Martinez-Sobrido</surname><given-names>L.</given-names></name></person-group><article-title>Rearrangement of influenza virus spliced segments for the development of live-attenuated vaccines</article-title><source>J. Virol.</source><year>2016</year><pub-id pub-id-type="doi">10.1128/JVI.00410-16</pub-id><pub-id pub-id-type="pmid">27122587</pub-id></element-citation></ref><ref id="B197-ijms-18-00020"><label>197.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Garcia-Sastre</surname><given-names>A.</given-names></name></person-group><article-title>H5N1, a wealth of knowledge to improve pandemic preparedness</article-title><source>Virus Res.</source><year>2013</year><volume>178</volume><fpage>1</fpage><lpage>2</lpage><pub-id pub-id-type="doi">10.1016/j.virusres.2013.11.001</pub-id><pub-id pub-id-type="pmid">24238658</pub-id></element-citation></ref><ref id="B198-ijms-18-00020"><label>198.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Perez</surname><given-names>D.R.</given-names></name><name><surname>Lim</surname><given-names>W.</given-names></name><name><surname>Seiler</surname><given-names>J.P.</given-names></name><name><surname>Yi</surname><given-names>G.</given-names></name><name><surname>Peiris</surname><given-names>M.</given-names></name><name><surname>Shortridge</surname><given-names>K.F.</given-names></name><name><surname>Webster</surname><given-names>R.G.</given-names></name></person-group><article-title>Role of quail in the interspecies transmission of H9 influenza A viruses: Molecular changes on HA that correspond to adaptation from ducks to chickens</article-title><source>J. Virol.</source><year>2003</year><volume>77</volume><fpage>3148</fpage><lpage>3156</lpage><pub-id pub-id-type="doi">10.1128/JVI.77.5.3148-3156.2003</pub-id><pub-id pub-id-type="pmid">12584339</pub-id></element-citation></ref><ref id="B199-ijms-18-00020"><label>199.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Deng</surname><given-names>L.</given-names></name><name><surname>Cho</surname><given-names>K.J.</given-names></name><name><surname>Fiers</surname><given-names>W.</given-names></name><name><surname>Saelens</surname><given-names>X.</given-names></name></person-group><article-title>M2e-based universal influenza A vaccines</article-title><source>Vaccines</source><year>2015</year><volume>3</volume><fpage>105</fpage><lpage>136</lpage><pub-id pub-id-type="doi">10.3390/vaccines3010105</pub-id><pub-id pub-id-type="pmid">26344949</pub-id></element-citation></ref><ref id="B200-ijms-18-00020"><label>200.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wilson</surname><given-names>I.A.</given-names></name><name><surname>Skehel</surname><given-names>J.J.</given-names></name><name><surname>Wiley</surname><given-names>D.C.</given-names></name></person-group><article-title>Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution</article-title><source>Nature</source><year>1981</year><volume>289</volume><fpage>366</fpage><lpage>373</lpage><pub-id pub-id-type="doi">10.1038/289366a0</pub-id><pub-id pub-id-type="pmid">7464906</pub-id></element-citation></ref><ref id="B201-ijms-18-00020"><label>201.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>H.</given-names></name><name><surname>Wang</surname><given-names>L.</given-names></name><name><surname>Compans</surname><given-names>R.W.</given-names></name><name><surname>Wang</surname><given-names>B.Z.</given-names></name></person-group><article-title>Universal influenza vaccines, a dream to be realized soon</article-title><source>Viruses</source><year>2014</year><volume>6</volume><fpage>1974</fpage><lpage>1991</lpage><pub-id pub-id-type="doi">10.3390/v6051974</pub-id><pub-id pub-id-type="pmid">24784572</pub-id></element-citation></ref><ref id="B202-ijms-18-00020"><label>202.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Song</surname><given-names>H.</given-names></name><name><surname>Wittman</surname><given-names>V.</given-names></name><name><surname>Byers</surname><given-names>A.</given-names></name><name><surname>Tapia</surname><given-names>T.</given-names></name><name><surname>Zhou</surname><given-names>B.</given-names></name><name><surname>Warren</surname><given-names>W.</given-names></name><name><surname>Heaton</surname><given-names>P.</given-names></name><name><surname>Connolly</surname><given-names>K.</given-names></name></person-group><article-title>In vitro stimulation of human influenza-specific CD8<sup>+</sup> T cells by dendritic cells pulsed with an influenza virus-like particle (VLP) vaccine</article-title><source>Vaccine</source><year>2010</year><volume>28</volume><fpage>5524</fpage><lpage>5532</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2010.06.044</pub-id><pub-id pub-id-type="pmid">20600506</pub-id></element-citation></ref><ref id="B203-ijms-18-00020"><label>203.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Latham</surname><given-names>T.</given-names></name><name><surname>Galarza</surname><given-names>J.M.</given-names></name></person-group><article-title>Formation of wild-type and chimeric influenza virus-like particles following simultaneous expression of only four structural proteins</article-title><source>J. Virol.</source><year>2001</year><volume>75</volume><fpage>6154</fpage><lpage>6165</lpage><pub-id pub-id-type="doi">10.1128/JVI.75.13.6154-6165.2001</pub-id><pub-id pub-id-type="pmid">11390617</pub-id></element-citation></ref><ref id="B204-ijms-18-00020"><label>204.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pushko</surname><given-names>P.</given-names></name><name><surname>Tumpey</surname><given-names>T.M.</given-names></name><name><surname>Bu</surname><given-names>F.</given-names></name><name><surname>Knell</surname><given-names>J.</given-names></name><name><surname>Robinson</surname><given-names>R.</given-names></name><name><surname>Smith</surname><given-names>G.</given-names></name></person-group><article-title>Influenza virus-like particles comprised of the HA, NA, and M1 proteins of H9N2 influenza virus induce protective immune responses in BALB/c mice</article-title><source>Vaccine</source><year>2005</year><volume>23</volume><fpage>5751</fpage><lpage>5759</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2005.07.098</pub-id><pub-id pub-id-type="pmid">16143432</pub-id></element-citation></ref><ref id="B205-ijms-18-00020"><label>205.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>B.J.</given-names></name><name><surname>Leser</surname><given-names>G.P.</given-names></name><name><surname>Morita</surname><given-names>E.</given-names></name><name><surname>Lamb</surname><given-names>R.A.</given-names></name></person-group><article-title>Influenza virus hemagglutinin and neuraminidase, but not the matrix protein, are required for assembly and budding of plasmid-derived virus-like particles</article-title><source>J. Virol.</source><year>2007</year><volume>81</volume><fpage>7111</fpage><lpage>7123</lpage><pub-id pub-id-type="doi">10.1128/JVI.00361-07</pub-id><pub-id pub-id-type="pmid">17475660</pub-id></element-citation></ref><ref id="B206-ijms-18-00020"><label>206.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pushko</surname><given-names>P.</given-names></name><name><surname>Pearce</surname><given-names>M.B.</given-names></name><name><surname>Ahmad</surname><given-names>A.</given-names></name><name><surname>Tretyakova</surname><given-names>I.</given-names></name><name><surname>Smith</surname><given-names>G.</given-names></name><name><surname>Belser</surname><given-names>J.A.</given-names></name><name><surname>Tumpey</surname><given-names>T.M.</given-names></name></person-group><article-title>Influenza virus-like particle can accommodate multiple subtypes of hemagglutinin and protect from multiple influenza types and subtypes</article-title><source>Vaccine</source><year>2011</year><volume>29</volume><fpage>5911</fpage><lpage>5918</lpage><pub-id pub-id-type="doi">10.1016/j.vaccine.2011.06.068</pub-id><pub-id pub-id-type="pmid">21723354</pub-id></element-citation></ref></ref-list></back><floats-group><fig id="ijms-18-00020-f001" position="float"><label>Figure 1</label><caption><p>Virion structure of IAV (<bold>A</bold>) and IBV (<bold>B</bold>): IAV and IBV are surrounded by a lipid bilayer containing the two viral glycoproteins hemagglutinin (HA), responsible for binding to sialic acid-containing receptors in the surface of susceptible cells, and neuraminidase (NA), responsible for viral release from infected cells. Furthermore, in the virion membrane is the ion channel M2 (IAV) or BM2 and NB (IBV) proteins. Under the viral lipid bilayer is a protein layer composed of the M1 protein, which plays a role in virion assembly and budding, and the nuclear export protein (NEP) involved in the nuclear export of the viral ribonucleoprotein (vRNP) complexes. The eight viral segments and protein products are indicated inside the virions. Black lines at the end of each of the eight IAV and IBV vRNAs indicate the 3′ and 5′ non-coding regions (NCR). PB1 and PB2, polymerase basic 1 and 2; PA, polymerase acid; NP, nucleoprotein; NS, nonstructural gene; M: matrix; BM2: influenza B matrix protein 2.</p></caption><graphic xlink:href="ijms-18-00020-g001"></graphic></fig><fig id="ijms-18-00020-f002" position="float"><label>Figure 2</label><caption><p>Schematic representation to produce inactivated (<bold>A</bold>) or live-attenuated (<bold>B</bold>) influenza vaccines by genetic reassortment in embryonated eggs: The traditional method for generating reassortant virus is based on the coinfection of two influenza viruses in eggs. Both the WHO candidate virus and the high-growth virus for influenza inactivated vaccine (IIV) (<bold>A</bold>) or the master donor virus (MDV) for live-attenuated influenza vaccine (LAIV) (<bold>B</bold>) are inoculated in eggs followed by the selection of appropriate seed viruses by amplification in the presence of antibodies against the HA and NA of the high-growth virus (<bold>A</bold>) or the MDV (<bold>B</bold>). The resulting viruses containing the HA and NA segments from the WHO-recommended strain and the six internal vRNAs of the high-growth virus (<bold>A</bold>) or the MDV (<bold>B</bold>) are used for vaccine production. PR8, Puerto Rico/8.</p></caption><graphic xlink:href="ijms-18-00020-g002"></graphic></fig><fig id="ijms-18-00020-f003" position="float"><label>Figure 3</label><caption><p>Influenza vRNA cloning into bi-directional rescue plasmids. (<bold>A</bold>) Schematic representation of an ambisense plasmid, influenza cDNA inserts and generation of influenza rescue plasmids: The ambisense plasmid is a bi-directional vector containing the human polymerase I promoter (hPol-I, white arrow) and the mouse Pol-I terminator (TI, white box) sequences to direct the synthesis of the influenza vRNAs. Transcription from the Pol-I cassette results in vRNAs identical to those present in influenza virus, allowing their recognition by the influenza polymerase complex. In opposite orientation to the Pol-I cassette, a polymerase II-dependent cytomegalovirus promoter (Pol-II, black arrow) and a polyadenylation sequence (aBGH, black box) direct the synthesis of influenza proteins from the same viral cDNAs; (<bold>B</bold>) Influenza plasmid-based reverse genetics: In cells transfected with the influenza ambisense plasmids, the Pol-I cassette generates the eight negative sense vRNAs (<bold>bottom</bold>) while the Pol-II directs the synthesis of the eight viral mRNAs (<bold>top</bold>) that are translated into the influenza viral proteins. After translation, influenza NP and polymerase complex PA, PB1 and PB2 associate with the vRNAs to form the viral ribonucleoprotein (vRNP) complexes and initiate transcription from the viral promoter located within the non-coding regions at the 3′ termini of the vRNAs. Transcription results in the synthesis of more mRNAs and proteins. The influenza polymerase complex also replicates the vRNAs into complementary (c)RNAs that serve as templates for the amplification of vRNAs. Newly-synthesized vRNAs, together with the structural viral proteins result in the formation of new influenza viruses. Blue and red boxes indicates the HA and NA of seasonal influenza viruses to be included in either the IIV or the LAIV vaccine, respectively. Amp<sup>r</sup>: ampicillin resistance gene; Ori: plasmid origin of replication.</p></caption><graphic xlink:href="ijms-18-00020-g003"></graphic></fig><fig id="ijms-18-00020-f004" position="float"><label>Figure 4</label><caption><p>Reverse genetics approaches to generate influenza vaccines: For the development of reverse genetics, influenza vRNAs are cloned into the eight bi-directional plasmids. Transfection of the eight ambisense plasmids into permissible FDA-approved for vaccine production cell lines leads to the rescue of the recombinant influenza viruses containing the six internal genome segments from the high-growth virus (<bold>A</bold>) or from the MDV (<bold>B</bold>) and two genome segments (the HA and NA encoding segments) from WHO candidate strain for their use as IIV (<bold>A</bold>) or LAIV (<bold>B</bold>), respectively. Because the viruses are derived entirely from DNA, no selection system is needed to isolate the desired reassortant. The rescued viruses can be amplified and used as seed viruses for vaccine production.</p></caption><graphic xlink:href="ijms-18-00020-g004"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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