Use it or lose it: establishment and persistence of T cell memory
Identifieur interne : 000964 ( Pmc/Corpus ); précédent : 000963; suivant : 000965Use it or lose it: establishment and persistence of T cell memory
Auteurs : Katherine Kedzierska ; Sophie A. Valkenburg ; Peter C. Doherty ; Miles P. Davenport ; Vanessa VenturiSource :
- Frontiers in Immunology [ 1664-3224 ] ; 2012.
Abstract
Pre-existing T cell memory provides substantial protection against viral, bacterial, and parasitic infections. The generation of protective T cell memory constitutes a primary goal for cell-mediated vaccines, thus understanding the mechanistic basis of memory development and maintenance are of major importance. The widely accepted idea that T cell memory pools are directly descended from the effector populations has been challenged by recent reports that provide evidence for the early establishment of T cell memory and suggest that the putative memory precursor T cells do not undergo full expansion to effector status. Moreover, it appears that once the memory T cells are established early in life, they can persist for the lifetime of an individual. This is in contrast to the reported waning of naïve T cell immunity with age. Thus, in the elderly, immune memory that was induced at an early age may be more robust than recently induced memory, despite the necessity for long persistence. The present review discusses the mechanisms underlying the early establishment of immunological memory and the subsequent persistence of memory T cell pools in animal models and humans.
Url:
DOI: 10.3389/fimmu.2012.00357
PubMed: 23230439
PubMed Central: 3515894
Links to Exploration step
PMC:3515894Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>Pre-existing T cell memory provides substantial protection against viral, bacterial, and parasitic infections. The generation of protective T cell memory constitutes a primary goal for cell-mediated vaccines, thus understanding the mechanistic basis of memory development and maintenance are of major importance. The widely accepted idea that T cell memory pools are directly descended from the effector populations has been challenged by recent reports that provide evidence for the early establishment of T cell memory and suggest that the putative memory precursor T cells do not undergo full expansion to effector status. Moreover, it appears that once the memory T cells are established early in life, they can persist for the lifetime of an individual. This is in contrast to the reported waning of naïve T cell immunity with age. Thus, in the elderly, immune memory that was induced at an early age may be more robust than recently induced memory, despite the necessity for long persistence. The present review discusses the mechanisms underlying the early establishment of immunological memory and the subsequent persistence of memory T cell pools in animal models and humans.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Immunol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Immunol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Immun.</journal-id>
<journal-title-group><journal-title>Frontiers in Immunology</journal-title>
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<issn pub-type="epub">1664-3224</issn>
<publisher><publisher-name>Frontiers Media S.A.</publisher-name>
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</journal-meta>
<article-meta><article-id pub-id-type="pmid">23230439</article-id>
<article-id pub-id-type="pmc">3515894</article-id>
<article-id pub-id-type="doi">10.3389/fimmu.2012.00357</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Immunology</subject>
<subj-group><subject>Review Article</subject>
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<title-group><article-title>Use it or lose it: establishment and persistence of T cell memory</article-title>
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<xref ref-type="aff" rid="aff1"><sup>1</sup>
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<xref ref-type="author-notes" rid="fn001"><sup>*</sup>
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<given-names>Miles P.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Venturi</surname>
<given-names>Vanessa</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup>
<institution>Department of Microbiology and Immunology, University of Melbourne</institution>
<country>Melbourne, VIC, Australia</country>
</aff>
<aff id="aff2"><sup>2</sup>
<institution>Department of Immunology, St Jude Children’s Research Hospital</institution>
<country>Memphis, TN, USA</country>
</aff>
<aff id="aff3"><sup>3</sup>
<institution>Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales</institution>
<country>Kensington, NSW, Australia</country>
</aff>
<aff id="aff4"><sup>4</sup>
<institution>Computational Biology Group, Centre for Vascular Research, University of New South Wales</institution>
<country>Kensington, NSW, Australia</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: <italic>Gabrielle Belz, Walter and Eliza Hall Institute of Medical Research, Australia</italic>
</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: <italic>Awen Gallimore, Cardiff University, UK; James Riley, University of Pennsylvania, USA</italic>
</p>
</fn>
<corresp id="fn001">*Correspondence: <italic>Katherine Kedzierska, Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Melbourne, VIC, Australia. e-mail: <email xlink:type="simple">kkedz@unimelb.edu.au</email>
</italic>
</corresp>
<fn fn-type="other" id="fn002"><p>This article was submitted to Frontiers in Immunological Memory, a specialty of Frontiers in Immunology.</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>27</day>
<month>11</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="collection"><year>2012</year>
</pub-date>
<volume>3</volume>
<elocation-id>357</elocation-id>
<history><date date-type="received"><day>01</day>
<month>10</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>08</day>
<month>11</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © Kedzierska, Valkenburg, Doherty, Davenport and Venturi.</copyright-statement>
<copyright-year>2012</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.</license-p>
</license>
</permissions>
<abstract><p>Pre-existing T cell memory provides substantial protection against viral, bacterial, and parasitic infections. The generation of protective T cell memory constitutes a primary goal for cell-mediated vaccines, thus understanding the mechanistic basis of memory development and maintenance are of major importance. The widely accepted idea that T cell memory pools are directly descended from the effector populations has been challenged by recent reports that provide evidence for the early establishment of T cell memory and suggest that the putative memory precursor T cells do not undergo full expansion to effector status. Moreover, it appears that once the memory T cells are established early in life, they can persist for the lifetime of an individual. This is in contrast to the reported waning of naïve T cell immunity with age. Thus, in the elderly, immune memory that was induced at an early age may be more robust than recently induced memory, despite the necessity for long persistence. The present review discusses the mechanisms underlying the early establishment of immunological memory and the subsequent persistence of memory T cell pools in animal models and humans.</p>
</abstract>
<kwd-group><kwd>T cell memory</kwd>
<kwd>CD8 T cells</kwd>
<kwd>aged</kwd>
<kwd>Influenza A virus</kwd>
<kwd>CD4<sup>+</sup>
T lymphocytes</kwd>
</kwd-group>
<counts><fig-count count="3"></fig-count>
<table-count count="0"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="110"></ref-count>
<page-count count="11"></page-count>
<word-count count="0"></word-count>
</counts>
</article-meta>
</front>
<body><sec><title>INTRODUCTION</title>
<p>Memory T cells provide protection against re-infection with the same pathogen enabling a more rapid recovery of the host and a milder clinical outcome (<xref ref-type="bibr" rid="B77">Powell et al., 2007</xref>
). For example, survival from the H2N2 influenza pandemic of 1957 was attributed to pre-existing T cell immunity in the adult population (<xref ref-type="bibr" rid="B34">Epstein, 2006</xref>
). Furthermore, high levels of pre-existing CD4<sup>+</sup>
T cell memory correlate with reduced viral loads and clinical scores in a human influenza challenge study, highlighting the importance of T cell memory in viral infection (<xref ref-type="bibr" rid="B107">Wilkinson et al., 2012</xref>
). Once established, memory T cells can reside in tissues or recirculate between the blood, organs, and lymph nodes. These memory T cells appear to be quite distinct from the recently described tissue resident memory cells (Trm; <xref ref-type="bibr" rid="B35">Gebhardt et al., 2009</xref>
) displaying a unique set of molecular markers and optimal functions within the tissues (<xref ref-type="bibr" rid="B61">Mackay et al., 2012</xref>
; <xref ref-type="bibr" rid="B102">Wakim et al., 2012</xref>
).</p>
<p>For a long time, the traditional view of T cell memory formation involved the expansion of pathogen-specific effector T cells in number in response to infection followed by an extensive contraction in cell numbers (~90–95%), leaving a residual stable memory T cell pool, known as the linear model of memory differentiation. It has been known for decades that CD8<sup>+</sup>
memory T cells sort into CD62L<sup>hi</sup>
and CD62L<sup>lo</sup>
sets (<xref ref-type="bibr" rid="B98">Tripp et al., 1995</xref>
), with the former having the capacity to recirculate from blood to lymph nodes via the high endothelial venules, while the latter move readily through other tissue compartments and can return to the lymph node via the afferent lymphatics. Thirteen years ago, a seminal paper by <xref ref-type="bibr" rid="B86">Sallusto et al. (1999)</xref>
coined the terms “central” (CD62L<sup>hi</sup>
) and “effector” (CD62L<sup>lo</sup>
) to describe these memory T cell subsets. These findings challenged the traditional view of memory formation, suggesting a more complex process, and raised important questions about the generation of T cell memory and which populations actually persist for the life-time of an individual. In fact, the key question has long been whether persistent T cells (CD62L<sup>hi</sup>
or CD62L<sup>lo</sup>
) are survivors from the clonally expanded effector cytotoxic T lymphocyte (CTL) pool or are part of a separate lineage.</p>
<p>More than a decade of research and published studies provide evidence of the early establishment of memory, which can be greatly affected by the inflammatory stimuli and transcription signatures at the acute phase of the infection. Both animal and human studies also show that T cell memory is long-lived and can be detected for up to 75 years in humans (<xref ref-type="bibr" rid="B38">Hammarlund et al., 2003</xref>
) and for the life-time of a laboratory mouse (<xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
). A growing area of research focused on the impact of age on the immune system suggests that age-related changes have potential implications for the generation and maintenance of T cell memory late in life. Interestingly, memory T cell populations directed at acute and long-term persistent infections exhibit different functions and characteristics, as the latter display signs of exhaustion due to the persistence of the antigen. In addition, studies suggest that, in the context of acute infections, the protective capacity of memory CD8<sup>+</sup>
T cells is pathogen-specific and can be substantially impacted by repeated antigenic stimulation (<xref ref-type="bibr" rid="B71">Nolz and Harty, 2011</xref>
). In this review, we discuss both the establishment and persistence of primary T cell memory, with a particular focus on CD8<sup>+</sup>
T cell responses directed at acute readily resolved infections such as respiratory viruses.</p>
</sec>
<sec><title>ESTABLISHMENT OF T CELL MEMORY</title>
<p>The relatively recent development of the tetramer-magnetic enrichment approach (<xref ref-type="bibr" rid="B68">Moon et al., 2007</xref>
) has substantially shaped our understanding of the recruitment, expansion, and persistence of endogenous T cell numbers for the naïve, effector, and memory T cell populations. The numbers of naïve T cells per mouse available to respond to a specific antigenic epitope range from tens (e.g., ≈30 naïve precursors for the immunodominant influenza-specific D<sup>b</sup>
NP<sub>366</sub>
epitope; <xref ref-type="bibr" rid="B58">La Gruta et al., 2010</xref>
) to hundreds (e.g., ≈600 for the murine cytomegalovirus D<sup>b</sup>
M45 epitope; <xref ref-type="bibr" rid="B72">Obar and Lefrancois, 2010</xref>
). After antigenic stimulation, the naïve precursors increase in prevalence by more than 10,000 times (<xref ref-type="bibr" rid="B25">Croom et al., 2011</xref>
), whilst substantially fewer cells establish stable memory pools. These memory pools increase the numbers of antigen-specific T cells by up to 200–1000 times (<xref ref-type="bibr" rid="B13">Belz et al., 2000</xref>
; <xref ref-type="bibr" rid="B42">Hogan et al., 2001</xref>
; <xref ref-type="bibr" rid="B62">Marshall et al., 2001</xref>
; <xref ref-type="bibr" rid="B25">Croom et al., 2011</xref>
) compared to the initial naïve precursor pool (<xref ref-type="bibr" rid="B58">La Gruta et al., 2010</xref>
). Such effects are readily observed in mouse model systems following prime/challenge with influenza A viruses that differ for their surface H and N proteins but share internal components (<xref ref-type="bibr" rid="B54">Kedzierska et al., 2008</xref>
). It has been reported that the size of antigen-specific memory pools strongly correlates with the level of immune protection (<xref ref-type="bibr" rid="B7">Badovinac et al., 2003</xref>
; <xref ref-type="bibr" rid="B88">Schmidt et al., 2008</xref>
). As the establishment of immunological memory is critical for a rational design of any cell-mediated vaccine, understanding the precise mechanisms of memory formation is essential.</p>
<sec><title>EARLY ESTABLISHMENT OF T CELL MEMORY</title>
<p>Recent studies provide important insights into the development of memory T cells. Experiments using <italic>in vitro</italic>
stimulated T cells (<xref ref-type="bibr" rid="B48">Kaech and Ahmed, 2001</xref>
), antibiotic treatment prior to <italic>Listeria monocytogenes</italic>
or influenza co-infection (<xref ref-type="bibr" rid="B9">Badovinac et al., 2004</xref>
), dendritic-cell vaccination (<xref ref-type="bibr" rid="B8">Badovinac et al., 2005</xref>
), clonal dissection of influenza-specific CD8<sup>+</sup>
T cells at different stages of infection (<xref ref-type="bibr" rid="B53">Kedzierska et al., 2006</xref>
, <xref ref-type="bibr" rid="B54">2008</xref>
) or transfer studies (<xref ref-type="bibr" rid="B52">Kedzierska et al., 2007</xref>
) suggest that the full expansion to effector status is not a pre-requisite for the generation of long-term memory T cells.</p>
<p>As mentioned previously, the discovery that memory T cell populations are comprised of distinct T cell subsets (<xref ref-type="bibr" rid="B86">Sallusto et al., 1999</xref>
) has also had a substantial impact on our understanding of T cell memory. Based on the expression of lymph-node homing markers (CD62L and CCR7), memory T cells have been classified as central memory T cells (T<sub>CM</sub>
; CD62L<sup>hi</sup>
, CCR7<sup>hi</sup>
), circulating between lymphoid organs, and effector memory (T<sub>EM</sub>
, CD62L<sup>lo</sup>
, CCR7<sup>lo</sup>
), found principally in the blood, spleen, and peripheral organs (<xref ref-type="bibr" rid="B86">Sallusto et al., 1999</xref>
; <xref ref-type="bibr" rid="B63">Masopust et al., 2001</xref>
; <xref ref-type="bibr" rid="B78">Reinhardt et al., 2001</xref>
). These T<sub>CM</sub>
and T<sub>EM</sub>
memory populations are also considered to differ at the functional level. While human T<sub>EM</sub>
cells display immediate cytotoxic activity <italic>ex vivo</italic>
, T<sub>CM</sub>
populations acquire effector function after short-term <italic>in vitro</italic>
stimulation (<xref ref-type="bibr" rid="B86">Sallusto et al., 1999</xref>
; <xref ref-type="bibr" rid="B63">Masopust et al., 2001</xref>
). Although both subsets can produce anti-viral cytokines IFN-γ and TNF-α, IL-2 production is largely limited to CD62L<sup>hi</sup>
memory cells (<xref ref-type="bibr" rid="B106">Wherry et al., 2003</xref>
). Significant insights into T cell formation and maintenance have been gained since studies have focused on classifying T<sub>EM</sub>
and T<sub>CM</sub>
cells.</p>
<p>A first important observation from studies of the T<sub>EM</sub>
and T<sub>CM</sub>
subsets is that the proportions of T<sub>EM</sub>
and T<sub>CM</sub>
populations change throughout the course of infection. In contrast to naïve CD8<sup>+</sup>
T cells expressing the CD62L<sup>hi</sup>
CD44<sup>lo</sup>
phenotype, the majority of CD8<sup>+</sup>
T cells present at the peak of infection display the surface markers CD62L<sup>lo</sup>
CD44<sup>hi</sup>
, although some (around 10%; <xref ref-type="bibr" rid="B53">Kedzierska et al., 2006</xref>
) remain of the CD62L<sup>hi</sup>
CD44<sup>hi</sup>
phenotype (<bold>Figure <xref ref-type="fig" rid="F1">1</xref>
</bold>
). As the virus is cleared and memory is established, the proportion of cells with the CD62L<sup>hi</sup>
phenotype progressively increases. For example, around 50% of the influenza-specific immunodominant D<sup>b</sup>
NP<sub>366</sub>
<sup>+</sup>
CD8<sup>+</sup>
and D<sup>b</sup>
PA<sub>224</sub>
<sup>+</sup>
CD8<sup>+</sup>
T cell memory populations express CD62L<sup>hi</sup>
at d60 after infection, compared with 90% being CD62L<sup>hi</sup>
by d500 (<xref ref-type="bibr" rid="B53">Kedzierska et al., 2006</xref>
). Interestingly, the numbers of CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cells remain relatively stable throughout the infection, while the total numbers of CD62L<sup>lo</sup>
CD8<sup>+</sup>
T cells rapidly decreases during the memory phase (<xref ref-type="bibr" rid="B87">Schlub et al., 2010</xref>
; <bold>Figure <xref ref-type="fig" rid="F1">1A</xref>
</bold>
). This suggests that, rather than there being a regression from CD62L<sup>lo</sup>
to CD62L<sup>hi</sup>
, there is preferential survival of antigen-specific memory CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cells (T<sub>CM</sub>
) set. This can be also translated into the relative contributions of CD62L<sup>lo</sup>
and CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cell memory populations to the secondary response to Sendai virus (<xref ref-type="bibr" rid="B80">Roberts and Woodland, 2004</xref>
; <xref ref-type="bibr" rid="B81">Roberts et al., 2005</xref>
). Although the recall responses by the CD62L<sup>lo</sup>
CD8<sup>+</sup>
T cell memory population dominate at the early memory phase of infection (d30), the central memory CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cells from later (1 year) memory time-points make a markedly greater contribution to recall responses (<xref ref-type="bibr" rid="B81">Roberts et al., 2005</xref>
).</p>
<fig id="F1" position="float"><label>FIGURE 1</label>
<caption><p><bold>Preferential survival of antigen-specific memory CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cells following influenza virus infection</bold>
. <bold>(A)</bold>
The numbers of influenza-specific CD62L<sup>hi</sup>
CD8<sup>+</sup>
T cells, but not CD62L<sup>lo</sup>
CD8<sup>+</sup>
T cell sets, are stable following the primary infection. <bold>(B)</bold>
This is in contrast to the relative frequencies of CD62L<sup>lo</sup>
and CD62L<sup>hi</sup>
effector and memory T cell populations as based on our published data in <xref ref-type="bibr" rid="B53">Kedzierska et al. (2006)</xref>
.</p>
</caption>
<graphic xlink:href="fimmu-03-00357-g001"></graphic>
</fig>
<p>Secondly, important insights into the early establishment of T cell memory have been made possible by the differential expression of CD62L<sup>lo</sup>
and CD62L<sup>hi</sup>
throughout the memory phase, which allows the tracking of different subsets starting from the early days after infection. Experiments utilizing transgenic mice suggest that it is possible to transition from the “more activated” CD62L<sup>lo</sup>
to the “less activated” CD62L<sup>hi</sup>
phenotype, indicating that being CD62L<sup>lo</sup>
is not a marker of terminal differentiation and supporting the possibility that there can be a linear model of T<sub>EM</sub>
to T<sub>CM</sub>
memory generation (<xref ref-type="bibr" rid="B105">Wherry et al., 2002</xref>
). The results of clonotypic analysis of T cell receptor (TCR) signatures in mice (<xref ref-type="bibr" rid="B17">Bouneaud et al., 2005</xref>
; <xref ref-type="bibr" rid="B53">Kedzierska et al., 2006</xref>
) and humans (<xref ref-type="bibr" rid="B10">Baron et al., 2003</xref>
) further expand this model. The indications are that although common TCRs are found in both T<sub>CM</sub>
and T<sub>EM</sub>
populations, the T<sub>CM</sub>
set contains additional TCR clonotypes not found within the T<sub>EM</sub>
set and that TCR diversity within both the T<sub>CM</sub>
and T<sub>EM</sub>
subsets appears to be stable. In mice, such divergent (and consistent) TCR repertoire characteristics are apparent for the T<sub>CM</sub>
and T<sub>EM</sub>
sets from the early acute phase of infection (d8) through to the long-term memory (>d500; <bold>Figure <xref ref-type="fig" rid="F2">2</xref>
</bold>
). This indeed suggests the early establishment of clonotypically stable T cell memory pools. In a follow-up study, we found that influenza-specific CD8<sup>+</sup>
T cells isolated on d3.5 after influenza infection, especially cells isolated from the draining lymph nodes, could survive following the transfer into naïve mice and be recalled after a secondary challenge (<xref ref-type="bibr" rid="B52">Kedzierska et al., 2007</xref>
). This suggests that stable CD8<sup>+</sup>
T cell memory is established early in the antigen-driven phase of influenza virus infection. The survival of memory CD8<sup>+</sup>
T cells isolated early after infection on d3.5 highly resembled the survival of CD8<sup>+</sup>
T cells isolated during the memory phase (d28; <bold>Figure <xref ref-type="fig" rid="F3">3</xref>
</bold>
). Conversely, when CD8<sup>+</sup>
T cells isolated at d8 (i.e., at the peak of the acute phase) were transferred into a naïve animal, the survival potential was lower than those of cells isolated early after the infection. This most likely reflects the high proportion of terminally differentiated short-lived effectors at the peak of the response to influenza viruses (<bold>Figure <xref ref-type="fig" rid="F3">3</xref>
</bold>
). Overall, these findings support the idea that antigen-specific CD8<sup>+</sup>
T cells that have not achieved full effector potential are more likely to be part of the memory pool. For example, CD8<sup>+</sup>
T cells lacking the cytotoxic capacity thus protected from cell damage during target cell killing. In addition, early d3.5 CD8<sup>+</sup>
T cells lacking CD25 (the IL-2Rα subunit) displayed enhanced survival into memory (<xref ref-type="bibr" rid="B52">Kedzierska et al., 2007</xref>
). This was further confirmed, more recently (<xref ref-type="bibr" rid="B11">Belz and Masson, 2010</xref>
; <xref ref-type="bibr" rid="B50">Kalia et al., 2010</xref>
; <xref ref-type="bibr" rid="B75">Pipkin et al., 2010</xref>
) in studies which showed lower CD25 expression, and thus weaker IL-2 signaling, leads to preferential generation of T cells with a memory phenotype capable of long-term survival.</p>
<fig id="F2" position="float"><label>FIGURE 2</label>
<caption><p><bold>Early establishment of a stable TCR repertoire composition for memory T<sub>EM</sub>
CD62L<sup>lo</sup>
and T<sub>CM</sub>
CD62L<sup>hi</sup>
sets</bold>
. Schematic summarizing the results of TCR repertoire analysis of the T<sub>EM</sub>
CD62L<sup>lo</sup>
and T<sub>CM</sub>
CD62L<sup>hi</sup>
subsets for influenza-specific D<sup>b</sup>
NP<sub>366</sub>
<sup>+</sup>
and D<sup>b</sup>
PA<sub>224</sub>
<sup>+</sup>
CD8<sup>+</sup>
T cells at the acute phase of infection (d8–d15), early memory (d28) and late memory (d180–d500). Data are based on <italic>n</italic>
= 3018 sequences published in <xref ref-type="bibr" rid="B53">Kedzierska et al. (2006)</xref>
.</p>
</caption>
<graphic xlink:href="fimmu-03-00357-g002"></graphic>
</fig>
<fig id="F3" position="float"><label>FIGURE 3</label>
<caption><p><bold>Early establishment and recall of memory precursors in an OT-I transgenic system</bold>
. Diagrammatic representation of findings published in <xref ref-type="bibr" rid="B52">Kedzierska et al. (2007)</xref>
. OT-I cells were labeled with CFSE, transferred into congenically different B6 mice and infected with an influenza virus expressing the SIINFEKL peptide in the viral NA. On d3.5, d8, or d28 after infection, OT-I CD44<sup>hi</sup>
cells, which have divided, were transferred into naïve B6 mice. After the following 40 days, mice were infected with influenza expressing SIINFEKL to recall any surviving memory cells. OT-I cells from d3.5 after influenza infection could survive and be recalled, findings which reflected the d28 (memory) time-point. The memory potential of d8 effectors was greatly diminished, most likely due to a high proportion of terminally differentiated effectors.</p>
</caption>
<graphic xlink:href="fimmu-03-00357-g003"></graphic>
</fig>
<p>Expression of the IL-7R α-chain (CD127) on CD8<sup>+</sup>
T cells during the acute phase of the response is also essential, although not sufficient on its own, for identifying the memory precursors at the acute time of infection (<xref ref-type="bibr" rid="B49">Kaech et al., 2003</xref>
; <xref ref-type="bibr" rid="B43">Huster et al., 2004</xref>
; <xref ref-type="bibr" rid="B39">Hand et al., 2007</xref>
; <xref ref-type="bibr" rid="B25">Croom et al., 2011</xref>
). Evidence for this comes from studies showing that, IL-7Rα-expressing cells at the peak of LCMV infection survive preferentially to give long-lived memory and show increased expression of anti-apoptotic molecules (such as bcl-2; <xref ref-type="bibr" rid="B49">Kaech et al., 2003</xref>
). Further, FACS separation of LCMV-specific effector CD8<sup>+</sup>
T cells on the basis of IL-7Rα and KLRG1 expression led to characterization of the IL-7Rα<sup>hi</sup>
KLRG1<sup>lo</sup>
memory precursor effector cells (MPEC), as distinct fromIL-7Rα<sup>lo</sup>
KLRG1<sup>hi</sup>
short-lived effector cells (SLEC) following LCMV infection (<xref ref-type="bibr" rid="B47">Joshi et al., 2007</xref>
). Acquisition of this memory IL-7Rα<sup>hi</sup>
KLRG1<sup>lo</sup>
phenotype in models of rapid, systemic infection (LCMV and <italic>L. monocytogenes</italic>
) depends on the spectrum of inflammatory cytokines present during the acute phase of the disease process (<xref ref-type="bibr" rid="B47">Joshi et al., 2007</xref>
; <xref ref-type="bibr" rid="B74">Pham et al., 2009</xref>
).</p>
<p>In influenza infection, a substantial number of antigen-specific D<sup>b</sup>
NP<sub>366</sub>
<sup>+</sup>
CD8<sup>+</sup>
and D<sup>b</sup>
PA<sub>224</sub>
<sup>+</sup>
CD8<sup>+</sup>
T cells express IL-7Rα at the peak of the acute primary response (70.2 ± 1.5 and 37.5 ± 2.4%, respectively), which does not translate into the relative numbers of T cells in the stable memory pools (<xref ref-type="bibr" rid="B25">Croom et al., 2011</xref>
). Thus, at least for influenza in a non-TCR-transgenic B6 mouse system, the majority of the antigen-specific IL-7Rα<sup>+</sup>
CD8<sup>+</sup>
T cells do not survive into the memory phase in a non-TCR-transgenic B6 mouse system. Further subsetting of influenza-specific CD8<sup>+</sup>
T cells into the SLEC and MPEC sets showed that, although the IL-7Rα<sup>hi</sup>
KLRG1<sup>lo</sup>
CD8<sup>+</sup>
population recovered from the spleen and the infected respiratory tract showed evidence of enhanced survival, the number of cells of this phenotype at peak does not define memory numbers. Our data suggested that the most stable MPEC IL-7Rα<sup>hi</sup>
KLRG1<sup>lo</sup>
numbers are observed in the draining mediastinal LN (MLN, draining the lungs), providing further evidence that, following this localized respiratory infection, the draining MLN offers the optimal anatomical niche for memory establishment and maintenance after influenza infection. Furthermore, the IL-7Rα<sup>hi</sup>
KLRG1<sup>lo</sup>
phenotype (but not the ultimate size of the memory pool) can be altered by varying the antigen dose, antigen presentation, extent of T cell division, and CD8<sup>+</sup>
T cell precursor numbers (<xref ref-type="bibr" rid="B25">Croom et al., 2011</xref>
). This indicates that cell surface molecule expression on CD8<sup>+</sup>
T cells predominantly reflects early antigenic experience and to a lesser extent marks the capacity to generate CD8<sup>+</sup>
T cell memory. And obviously, the potential of the memory cells to survive can be coupled to its replicative capacity (<xref ref-type="bibr" rid="B41">Hikono et al., 2007</xref>
).</p>
<p>In addition to the important determinants of T cell memory generation discussed above, sufficient CD4<sup>+</sup>
T cell help is also required for the establishment of stable memory (but not effector) influenza-specific CD8<sup>+</sup>
T cell populations. This was established by the diminished magnitude of memory and recall CD8<sup>+</sup>
T cell responses in MHC class II<sup>-</sup>
(IA<sup>b</sup>
<sup>-</sup>
<sup>/</sup>
<sup>-</sup>
) mice primed and then challenged with the influenza viruses (<xref ref-type="bibr" rid="B97">Topham et al., 1996</xref>
; <xref ref-type="bibr" rid="B28">Doherty and Christensen, 2000</xref>
; <xref ref-type="bibr" rid="B79">Riberdy et al., 2000</xref>
; <xref ref-type="bibr" rid="B12">Belz et al., 2002</xref>
), and mice treated with monoclonal antibody against CD4<sup>+</sup>
T cells (<xref ref-type="bibr" rid="B64">McKinstry et al., 2012</xref>
; <xref ref-type="bibr" rid="B94">Swain et al., 2012</xref>
). Unhelped CD8<sup>+</sup>
T cells have reduced survival to memory and reduced expansion during recall. The mechanism proposed involves help from CD4<sup>+</sup>
T cells resulting in down-regulation of TNF-α related apoptosis inducing ligand (TRAIL) on CD8<sup>+</sup>
T cells (<xref ref-type="bibr" rid="B45">Janssen et al., 2005</xref>
; <xref ref-type="bibr" rid="B6">Badovinac et al., 2006</xref>
).</p>
</sec>
<sec><title>THE ROLE OF INFLAMMATION AND TRANSCRIPTION FACTORS IN THE GENERATION OF STABLE MEMORY CD8<sup>+</sup>
T CELL POOLS</title>
<p>Infection-induced inflammation can affect T cell immunity at any stage. Evidence suggests that the fate of memory precursors can be determined by the nature of inflammatory stimuli during the antigen driven phase. Elegant studies using antibiotic treatment prior to <italic>L. monocytogenes</italic>
infection showed that reduced inflammation (including diminished IFN-γ production) markedly decreased T cell contraction (<xref ref-type="bibr" rid="B9">Badovinac et al., 2004</xref>
). Conversely, increasing inflammation with CpG treatment restored this effector T cell editing process (<xref ref-type="bibr" rid="B9">Badovinac et al., 2004</xref>
). These findings suggest that T cell memory pools can be generated independent of whether there is massive contraction in numbers. Furthermore, IL-12 has been shown to affect the progression to MPEC status via decreased T-bet expression (<xref ref-type="bibr" rid="B47">Joshi et al., 2007</xref>
). While low inflammation (low T-bet expression) promotes MPEC formation, high inflammation (T-bet expression) leads to numerically enhanced SLEC population. Thus, it appears that the inflammatory milieu, and particularly IFN-γ and IL-12, impact the proportion of cells expressing effector and memory-like markers at peak, and therefore the establishment of T cell memory pools. However, the exact mechanisms by which inflammation affects memory formation are yet to be elucidated.</p>
<p>Studies using peptide-pulsed dendritic cells revealed that antigenic stimulation of T cells without high-level inflammation typically present in infection, led to the earlier establishment of T cell memory, both phenotypically and functionally (<xref ref-type="bibr" rid="B8">Badovinac et al., 2005</xref>
). Again, this early establishment of memory pools was reversed by the addition of CpG. However, it is important to note that some level of inflammation is needed for T cell memory formation (<xref ref-type="bibr" rid="B90">Shaulov and Murali-Krishna, 2008</xref>
). There is a substantial body of evidence that the inflammatory stimuli can affect the molecular signatures of the responding T cells. The expression of T-bet, as mentioned above, can clearly direct antigen-specific T cells into either a memory or an effector pathway (<xref ref-type="bibr" rid="B47">Joshi et al., 2007</xref>
). Similarly, modulation of transcription factors such as Blimp-1 (<xref ref-type="bibr" rid="B51">Kallies et al., 2009</xref>
; <xref ref-type="bibr" rid="B85">Rutishauser et al., 2009</xref>
; <xref ref-type="bibr" rid="B91">Shin et al., 2009</xref>
) and Eomes (<xref ref-type="bibr" rid="B44">Intlekofer et al., 2005</xref>
) is necessary for the acquisition of effector versus memory lineage commitment. The main cytokines that affect the expression of theses transcription factors for responding T cells are IL-12, IL-2, IL-15, and IL-7, and the type-1 interferons. Dissecting the difference between the “good inflammation” necessary for the establishment of immunological memory versus the “bad inflammation” that leads to damaging immunopathology is imperative for future vaccine design.</p>
</sec>
</sec>
<sec><title>LONG-TERM PERSISTENCE OF T CELL MEMORY</title>
<sec><title>INSIGHTS GAINED FROM STUDIES IN AGED ANIMALS</title>
<p>Animal models have provided important insights into the persistence of T cell memory. In particular, experiments in aged animals allow a controlled analysis of the persistence of memory T cells over the lifetime that may follow an initial exposure to, and recovery from, a pathogenic infection. Such studies also allow us to study the recall of these memory cell populations after a subsequent pathogen challenge. Aged mice and non-human primates, similar to the elderly human population, are particularly susceptible to novel viral and bacterial infections, leading to an increase in the occurrence of severe disease. Thus, the impact of age on T cell responsiveness to infection or vaccination, together with the potential implications for both memory persistence into old age and the <italic>de novo</italic>
generation of such memory in the elderly, are priority areas for analysis.</p>
<p>The reduced efficacy of T cell responses to infection and vaccination with age is thought to reflect a decline in both naïve T cell numbers and TCR diversity, together with the functional compromise of the “aged” naïve T cells. The thymic epithelium involutes with age, substantially reducing the generation and export of naïve T cells (<xref ref-type="bibr" rid="B110">Yunis et al., 1973</xref>
; <xref ref-type="bibr" rid="B92">Simpson et al., 1975</xref>
). Thus, maintenance of the naïve T cell pool becomes more dependent on homeostatic turnover, evidenced by clonal expansions in the naïve T cell pool (<xref ref-type="bibr" rid="B60">LeMaoult et al., 2000</xref>
; <xref ref-type="bibr" rid="B21">Cicin-Sain et al., 2007</xref>
; <xref ref-type="bibr" rid="B2">Ahmed et al., 2009</xref>
) and the acquisition of a “memory-like” CD44<sup>hi</sup>
phenotype in older mice (<xref ref-type="bibr" rid="B37">Haluszczak et al., 2009</xref>
; <xref ref-type="bibr" rid="B84">Rudd et al., 2011b</xref>
; <xref ref-type="bibr" rid="B27">Decman et al., 2012</xref>
). These time-associated alterations to the naïve T cell pool are accompanied by an age-related increase in the proportion of memory T cells due to pathogen encounter, which is consistent with reported expansions in the memory pool with immunological experience (<xref ref-type="bibr" rid="B101">Vezys et al., 2009</xref>
). Moreover, large clonal expansions in the memory T cell repertoire associated with acute infection or primary vaccination (<xref ref-type="bibr" rid="B21">Cicin-Sain et al., 2007</xref>
; <xref ref-type="bibr" rid="B33">Ely et al., 2007</xref>
) and chronic infections are often observed (<xref ref-type="bibr" rid="B73">Ouyang et al., 2003</xref>
; <xref ref-type="bibr" rid="B33">Ely et al., 2007</xref>
; <xref ref-type="bibr" rid="B2">Ahmed et al., 2009</xref>
; <xref ref-type="bibr" rid="B56">Kohlmeier et al., 2010</xref>
). These factors collectively contribute to perturbations and a reduction in TCR diversity in the naïve T cell repertoire, which results in the preferential survival of high avidity TCR clonotypes (<xref ref-type="bibr" rid="B21">Cicin-Sain et al., 2007</xref>
; <xref ref-type="bibr" rid="B2">Ahmed et al., 2009</xref>
; <xref ref-type="bibr" rid="B84">Rudd et al., 2011b</xref>
; <xref ref-type="bibr" rid="B27">Decman et al., 2012</xref>
). As a consequence, aged individuals have impaired capacity to recruit an immune response a diverse array of TCR clonotypes when exposed to some novel infectious process (<xref ref-type="bibr" rid="B109">Yager et al., 2008</xref>
; <xref ref-type="bibr" rid="B83">Rudd et al., 2011a</xref>
; <xref ref-type="bibr" rid="B18">Bunztman et al., 2012</xref>
; <xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
), but is instead dominated by clonal expansions or reduced diversity relative to younger counterparts.</p>
<p>Apart from a decline in the number and diversity of naïve T cells, the “aged” naïve T cells are thought to be functionally defective, thus compromising the generation and persistence of T cell memory in aged individuals. Recent reviews on age-related changes for CD4<sup>+</sup>
(<xref ref-type="bibr" rid="B40">Haynes and Swain, 2012</xref>
) and CD8<sup>+</sup>
(<xref ref-type="bibr" rid="B70">Nikolich-Zugich et al., 2012</xref>
) T cell responses summarize our understanding of such functional defects in antigen-specific T cell responses in the “elderly.” These phenotypically obvious defects include reductions in the response magnitude, the capacity to proliferate and differentiate following activation, narrowed T cell polyfunctionality, and the increase in expression in inhibitory receptors associated typically with functional exhaustion. We refer readers to these excellent reviews in order to focus the remainder of this discussion on our recent finding concerning the generation and persistence of influenza-specific CD8<sup>+</sup>
T cell memory in aged mice.</p>
<p>Previous mouse studies have established that aging is associated with diminished CD8<sup>+</sup>
T cell efficacy and delayed influenza virus clearance (<xref ref-type="bibr" rid="B32">Effros and Walford, 1983</xref>
; <xref ref-type="bibr" rid="B14">Bender et al., 1991</xref>
; <xref ref-type="bibr" rid="B15">Bender et al., 1995</xref>
; <xref ref-type="bibr" rid="B30">Dong et al., 2000</xref>
; <xref ref-type="bibr" rid="B76">Po et al., 2002</xref>
). Recent evidence has further shown that the selective loss of primary, influenza-specific CD8<sup>+</sup>
T cell responsiveness in older mice is characterized by a narrowing in the spectrum of TCR usage that is seen predominantly for low frequency populations, with this effect being best characterized for the prominent D<sup>b</sup>
NP<sub>366</sub>
<sup>+</sup>
CD8<sup>+</sup>
T cell set (<xref ref-type="bibr" rid="B109">Yager et al., 2008</xref>
; <xref ref-type="bibr" rid="B96">Toapanta and Ross, 2009</xref>
; <xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
). Our work established that primary influenza virus infection of naïve 22-month (versus 12 week) old mice resulted in reduced numbers of immunodominant NP<sub>366</sub>
<sup>+</sup>
CD8<sup>+</sup>
and PA<sub>224</sub>
<sup>+</sup>
CD8<sup>+</sup>
T cells in the spleen compared with young adult mice (<xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
). In contrast, the difference in the magnitude for epitope-specific CD8<sup>+</sup>
T cell responses between young and old mice was not observed at the site of infection sampled by bronchoalveolar lavage (Valkenburg and Kedzierska, unpublished). This supports our previous findings that in the event of decreased T cell numbers, virus-specific CD8<sup>+</sup>
T cells traffic preferentially to the site of infection (<xref ref-type="bibr" rid="B99">Valkenburg et al., 2010</xref>
). However, despite there being comparable CD8<sup>+</sup>
T cell counts at the site of infection for young and old, the “aged” “inflammatory” CD8<sup>+</sup>
T cells displayed impaired cytokine profiles (Valkenburg and Kedzierska, unpublished), similar to those found for the spleen (<xref ref-type="bibr" rid="B99">Valkenburg et al., 2010</xref>
).</p>
<p>As a consequence, mice primed late (at 22 months) and then challenged even later in life (at 24 months) developed secondary CD8<sup>+</sup>
T cell responses that were diminished in quantity, quality, and TCR repertoire diversity (<xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
). This suggests that the memory CD8<sup>+</sup>
T cell pools established late in life are defective. Similarly, a recent study (<xref ref-type="bibr" rid="B26">Decman et al., 2010</xref>
) suggested that infecting mice with LCMV or influenza at an extreme age (18–20 months) leads to defective CD8<sup>+</sup>
T cell memory and diminished recall responses following virus challenge. Thus, the age at initial priming is a critical determinant of CTL numbers, diversity, and function, with memory CD8<sup>+</sup>
T cell populations that are generated later in life being generally less efficacious (<xref ref-type="bibr" rid="B31">Eaton et al., 2008</xref>
).</p>
<p>A key issue that then arises is whether we can recruit and maintain a pool of responsive T cells by priming those cells early in life, especially to diseases such as influenza, which are a particular threat to the elderly. We probed this question for influenza-specific CD8<sup>+</sup>
T cell memory. Our studies found that the ability to mount a CD8<sup>+</sup>
T cell response to influenza infection waned with age. However, early vaccination (at 6 weeks of age) prior to the attrition of low frequency anti-influenza CD8<sup>+</sup>
T cells was important to maintain the numbers, function, and a diverse array of TCRs of the memory pools for the life-time of an animal (<xref ref-type="bibr" rid="B100">Valkenburg et al., 2012</xref>
). The TCR repertoire in extremely aged mice was “locked in” at an early age following vaccination, which proved advantageous for providing a high avidity and high magnitude response later in life. Consequently, memory T cells generated by influenza priming at a young age had a better protective capacity that was evidenced by accelerated viral clearance and reduced body weight loss in the aged animals as compared to the aged mice responding to primary infection (Valkenburg and Kedzierska, unpublished data). Hence, it is important to establish influenza-specific CD8<sup>+</sup>
T cell responses early in life to preserve optimal, T cell responsiveness and protect against the age-related attrition of naïve T cell precursors. These findings also suggest that designing influenza vaccines, which promote as-broad-as-possible spectrum of CD8<sup>+</sup>
T cell memory in adolescence could be beneficial, even if such benefit emerges long after the subject has first seen the protective immunogen.</p>
</sec>
<sec><title>LONGEVITY OF HUMAN T CELL MEMORY</title>
<p>Novel insights into the longevity and function of human memory T cell responses have been provided by the elegant studies exploiting early live virus vaccination against yellow fever (YFV-17D) or smallpox (Dryvax; VV; reviewed in <xref ref-type="bibr" rid="B3">Ahmed and Akondy, 2011</xref>
). Immunization with YFV-17 enabled analysis of YFV-specific T cell responses for individuals residing in countries where they would have had no exposure to wild-type infection. Using this approach, longitudinal analyses showed that a single dose of YFV-17D immunization elicits potent effector CD8<sup>+</sup>
and CD4<sup>+</sup>
T cell responses that can then be maintained as long-lasting memory populations (<xref ref-type="bibr" rid="B23">Co et al., 2002</xref>
, <xref ref-type="bibr" rid="B22">2009</xref>
; <xref ref-type="bibr" rid="B4">Akondy et al., 2009</xref>
; <xref ref-type="bibr" rid="B108">Wrammert et al., 2009</xref>
). These memory sets had proportionally contracted from larger effector pools, co-incident to expression of CD127 (IL-7Rα; <xref ref-type="bibr" rid="B4">Akondy et al., 2009</xref>
). Importantly, YFV-specific T cell pools could be detected decades after immunization (<xref ref-type="bibr" rid="B3">Ahmed and Akondy, 2011</xref>
).</p>
<p>Similarly, exposure to vaccinia virus (VV), to protect against smallpox (or VV recombinants), has enabled the assessment of acute T cell responses and memory in the absence of secondary, “live virus” challenge (<xref ref-type="bibr" rid="B103">Walker and Slifka, 2010</xref>
). In-depth analysis of memory T cell persistence suggests that the estimated half-life (t<sub>1/2</sub>
) for CD8<sup>+</sup>
T cells is between 8 and 15 years, whereas t<sub>1/2</sub>
for the VV-specific CD4<sup>+</sup>
set is between 8 and 12 years (<xref ref-type="bibr" rid="B38">Hammarlund et al., 2003</xref>
; <xref ref-type="bibr" rid="B89">Seder and Ahmed, 2003</xref>
). At the peak of the acute VV-specific T cell responses, occurring within 2–3 weeks of human vaccination (<xref ref-type="bibr" rid="B95">Terajima et al., 2003</xref>
; <xref ref-type="bibr" rid="B55">Kennedy et al., 2004</xref>
; <xref ref-type="bibr" rid="B67">Miller et al., 2008</xref>
), the numbers of VV-specific effector CD8<sup>+</sup>
T cells are higher than those of CD4<sup>+</sup>
T cells within the same individual (<xref ref-type="bibr" rid="B5">Amara et al., 2004</xref>
; <xref ref-type="bibr" rid="B67">Miller et al., 2008</xref>
). However, a preferential loss of VV-specific CD8<sup>+</sup>
T cell memory is experienced by ~50% of individuals within 20 years post-vaccination (<xref ref-type="bibr" rid="B38">Hammarlund et al., 2003</xref>
). Interestingly, the t<sub>1/2</sub>
for T cells is much shorter than the t<sub>1/2</sub>
(92 years) for VV-specific antibody responses (<xref ref-type="bibr" rid="B103">Walker and Slifka, 2010</xref>
). However, though the T cell t<sub>1/2</sub>
appears to be <16 years, VV-specific T cells can be detected in some individuals for up to 75 years by IFN-γ/TNF-α staining (<xref ref-type="bibr" rid="B38">Hammarlund et al., 2003</xref>
). In comparison, measles virus (MV)-specific memory T cells induced after vaccination can be found for at least 34 years, with CD8<sup>+</sup>
T cell memory being more stable than CD4<sup>+</sup>
T cell memory (<xref ref-type="bibr" rid="B69">Naniche et al., 2004</xref>
). Taken together, the above studies with YFV, VV and MV suggest a long-term persistence of human T cell memory that is in every sense comparable to the profiles analyzed experimentally in mice.</p>
<p>The persistence of human CD8<sup>+</sup>
T cell memory after influenza virus infection is also of a particular interest, given the commonplace recurrence and the observed susceptibility in elderly populations. In contrast to strain-specific antibodies, T cells elicit broader immunity to a spectrum of influenza strains (seasonal and pandemic) as they generally recognize more conserved, internal components of the virus. Both CD4<sup>+</sup>
and CD8<sup>+</sup>
T cell sets play an important role in influenza-specific T cell-mediated immunity (<xref ref-type="bibr" rid="B66">McMichael et al., 1983b</xref>
; <xref ref-type="bibr" rid="B107">Wilkinson et al., 2012</xref>
). The effector CD8<sup>+</sup>
T cells function to recognize and destroy influenza virus-infected cells. Pre-existing CD8<sup>+</sup>
T cell memory promotes more rapid recovery via the production of pro-inflammatory cytokines and direct killing of virus-producing cells (<xref ref-type="bibr" rid="B29">Doherty et al., 1996</xref>
). Both experimental and epidemiological evidence indicates that memory CD8<sup>+</sup>
T cells primed by prior exposure to a seasonal influenza infection provide protection against subsequent challenge with novel, HA- and NA-distinct strains. Some virus replication still occurs, but the disease is less severe and the clinical outcome is improved. Evidence from animal models (<xref ref-type="bibr" rid="B20">Christensen et al., 2000</xref>
) and humans (<xref ref-type="bibr" rid="B66">McMichael et al., 1983b</xref>
; <xref ref-type="bibr" rid="B34">Epstein, 2006</xref>
; <xref ref-type="bibr" rid="B57">Kreijtz et al., 2008</xref>
; <xref ref-type="bibr" rid="B59">Lee et al., 2008</xref>
) supports this protective role for CD8<sup>+</sup>
T-cells following heterologous priming and challenge between H1N1, H7N7, H3N2, and H5N1 influenza viruses. However, persistence of influenza-specific memory T cells in humans is understudied, although early study by <xref ref-type="bibr" rid="B65">McMichael et al. (1983a)</xref>
used <sup>51</sup>
Cr-killing activity assay in PBMCs from individuals in 1977–1982 to infer contraction of T cell memory. It is, however, possible that a decrease in <sup>51</sup>
Cr lysis could be partially related to the loss of the killing capacity (<xref ref-type="bibr" rid="B93">Stambas et al., 2007</xref>
) or cytolytic molecule expression (gzm A, gzm B) in the long-term memory CD8<sup>+</sup>
T cells (<xref ref-type="bibr" rid="B46">Jenkins et al., 2007</xref>
) as shown previously by our studies in a mouse model of influenza infection. Similar observations have been made for human CD8<sup>+</sup>
T cells, with both granzyme A and granzyme B expression decreasing from 60% 1 month after infection to 33% within 1 year after infection (<xref ref-type="bibr" rid="B82">Rock et al., 2005</xref>
). Further studies epitope-specific CD8<sup>+</sup>
T cells (across different HLAs) using the more recent tetramer technology is needed to further explore this issue of memory CD8<sup>+</sup>
T cell persistence, and acute T cell responses, after human influenza virus infection.</p>
<p>Our recent antigenic analysis of the 2009-H1N1 virus, showed that this newly emerged pandemic virus shared immunogenic peptides with the catastrophic 1918-H1N1 strain (<xref ref-type="bibr" rid="B36">Gras et al., 2010</xref>
), and shared antigenic similarity with viruses present prior to 1945. This resulted in the detection of cross-reactive CD8<sup>+</sup>
T cell immunity between the H1N1-2009-infected donors and 1918 derived peptides for the NP<sub>418</sub>
epitope restricted by the large HLA-B7<sup>+</sup>
family. Cross-reactive immunity between the pandemic 2009-H1N1 strain and the 1918-H1N1 strain (as well as other H1N1 viruses from the beginning of the century) at both T cell and antibody levels may have resulted in lower susceptibility to the H1N1-2009 in individuals >65 years of age, with the majority of severe cases occurring in young adults (the mean patient age was 24 years; <xref ref-type="bibr" rid="B19">Cao et al., 2009</xref>
; <xref ref-type="bibr" rid="B1">Agrati et al., 2010</xref>
). This was in contrast to the usual scenario of the elderly population being more susceptible to annual, seasonal epidemics caused by influenza A viruses (<xref ref-type="bibr" rid="B24">Couch et al., 1986</xref>
; <xref ref-type="bibr" rid="B104">Webster, 2000</xref>
). Therefore, the lack of T cell immunity in younger adults and children, and the persistence of cross-reactive memory T cells in older individuals may partially account for the demographics of infection. The question thus still remains whether memory CD8<sup>+</sup>
T cell populations to influenza strains encountered early in life persist for a life-time.</p>
<p>A previous report on CD8<sup>+</sup>
T cell responses in elderly individuals showed no difference in the frequency of influenza-specific CTLs between the 18 and 70 years-old cohort, however there was lower lytic capacity in the 68–70 years versus the 18–20-year-old donors (<xref ref-type="bibr" rid="B16">Boon et al., 2002</xref>
). Then, while there were no significant differences in IFN-γ<sup>+</sup>
CD3<sup>+</sup>
CD8<sup>+</sup>
or CD4<sup>+</sup>
T cell numbers with age, the proportion of IL-4<sup>+</sup>
CD3<sup>+</sup>
CD8<sup>+</sup>
and IFN-γ<sup>+</sup>
CD3<sup>+</sup>
CD8<sup>+</sup>
(detected following PBMC stimulation with PMA/inomycin) increased in the elderly. Furthermore, the T cell proliferative response was significantly higher in the 18–20 years-old cohort, and was not antigen-dose dependent. That is, increasing antigen dose did not compensate for the reduced PBMC proliferation in 68–70 years-old individuals.</p>
<p>Interestingly, CD45RO (memory) and CD45RA (naïve) expression varied between different age groups. In the 18- to 20-year-old cohort, the prevalence of CD45RO versus CD45RA expression was similar for CD3<sup>+</sup>
CD4<sup>+</sup>
T cells, while a CD45RA > CD45RO distribution was found for the CD3<sup>+</sup>
CD8<sup>+</sup>
set. By contrast, the elderly (<xref ref-type="bibr" rid="B73">Ouyang et al., 2003</xref>
; <xref ref-type="bibr" rid="B109">Yager et al., 2008</xref>
; <xref ref-type="bibr" rid="B56">Kohlmeier et al., 2010</xref>
) group showed a CD45RO > CD45RA profile for both CD3<sup>+</sup>
CD4<sup>+</sup>
and CD3<sup>+</sup>
CD8<sup>+</sup>
T cells, together with reduced CD28 expression (for both CD4<sup>+</sup>
and CD8<sup>+</sup>
T cells) when compared with the youngsters. Furthermore, there was a trend for lower lytic capacity in the age 68–70 set following <italic>in vitro</italic>
PBMC stimulation (<xref ref-type="bibr" rid="B16">Boon et al., 2002</xref>
). This could be related to lower levels of perforin/granzyme expression. Overall, the analysis to date suggests that influenza-specific CD8<sup>+</sup>
T cells persist, though their cytolytic- and cytokine-producing potential may decline with increasing years. But the findings so far are limited in both scope and sophistication. There is clearly a need for much more detailed analysis of aging, influenza-specific human T cells, from the aspects of numbers, function, repertoire diversity, and capacity for effective recall.</p>
</sec>
</sec>
<sec><title>SUMMARY</title>
<p>The future design of T cell-based vaccination strategies that can provide effective and optimal protection across the lifespan of an individual crucially depends upon an in-depth understanding of the development and maintenance of T cell memory and the factors that impact the protective capacity of memory T cell populations. Studies in recent years have made substantial progress in dissecting the complexities of T cell memory populations, resulting in the identification of major factors that influence the composition and stability of these T cell memory populations. While these advances have moved us closer to elucidating the mechanisms contributing to optimal T cell memory generation, there remain many aspects of T cell memory to be investigated. Studies continue to reveal increased phenotypic, functional, and anatomical heterogeneity of T cell memory populations. The temporal changes to the composition and protective abilities of these T cell memory subsets require additional study in order to determine what constitutes optimal T cell memory. An important consideration in future investigations of T cell memory is the increasing evidence that the protective abilities of memory T cells are dependent on the pathogen and the nature of the infection. One potential complicating factor in the maintenance of life-long immunity is the changes at the cellular, environmental, and population level of T cells that occur in later life that have been associated with impaired immune responsiveness in the elderly. However, studies to date indicate that these age-related defects in T cell responses primarily affect the generation of T cell memory in old age. This suggests that to address the increased immune susceptibility in the elderly will require either the development of vaccines to be administered earlier in life, to generate T cell memory that will provide protection the against those pathogens likely to be encountered in later life, or the development of strategies to prevent or treat age-related defects in T cell immunity. Either approach provides substantial challenges for immunological research in terms of improving our understanding of temporal changes to T cell immunity.</p>
</sec>
<sec><title>Conflict of Interest Statement</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
</body>
<back><ack><p>This work was supported by Australian National Health and Medical Research Council (NHMRC) Project Grants to Katherine Kedzierska (AI1008854), an NHMRC Program Grant (APP567122) to Peter C. Doherty, an ARC Project Grant to Miles P. Davenport and Vanessa Venturi (DP0771340). Katherine Kedzierska is an NHMRC CDF2 Fellow, Sophie A. Valkenburg is an NHMRC Early Career Research Fellow, Vanessa Venturi is an ARC Future Fellow and Miles P. Davenport is an NHMRC Senior Research Fellow.</p>
</ack>
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