Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination</title><author><name sortKey="Lee, Jiwon" sort="Lee, Jiwon" uniqKey="Lee J" first="Jiwon" last="Lee">Jiwon Lee</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Boutz, Daniel R" sort="Boutz, Daniel R" uniqKey="Boutz D" first="Daniel R" last="Boutz">Daniel R. Boutz</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Chromikova, Veronika" sort="Chromikova, Veronika" uniqKey="Chromikova V" first="Veronika" last="Chromikova">Veronika Chromikova</name><affiliation><nlm:aff id="A3">Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</nlm:aff></affiliation></author><author><name sortKey="Joyce, M Gordon" sort="Joyce, M Gordon" uniqKey="Joyce M" first="M Gordon" last="Joyce">M Gordon Joyce</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Vollmers, Christopher" sort="Vollmers, Christopher" uniqKey="Vollmers C" first="Christopher" last="Vollmers">Christopher Vollmers</name><affiliation><nlm:aff id="A5">Department of Bioengineering, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Horton, Andrew P" sort="Horton, Andrew P" uniqKey="Horton A" first="Andrew P" last="Horton">Andrew P. Horton</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Dekosky, Brandon J" sort="Dekosky, Brandon J" uniqKey="Dekosky B" first="Brandon J" last="Dekosky">Brandon J. Dekosky</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Lee, Chang Han" sort="Lee, Chang Han" uniqKey="Lee C" first="Chang-Han" last="Lee">Chang-Han Lee</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Lavinder, Jason J" sort="Lavinder, Jason J" uniqKey="Lavinder J" first="Jason J" last="Lavinder">Jason J. Lavinder</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Murrin, Ellen M" sort="Murrin, Ellen M" uniqKey="Murrin E" first="Ellen M" last="Murrin">Ellen M. Murrin</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Chrysostomou, Constantine" sort="Chrysostomou, Constantine" uniqKey="Chrysostomou C" first="Constantine" last="Chrysostomou">Constantine Chrysostomou</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Hoi, Kam Hon" sort="Hoi, Kam Hon" uniqKey="Hoi K" first="Kam Hon" last="Hoi">Kam Hon Hoi</name><affiliation><nlm:aff id="A6">Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Tsybovsky, Yaroslav" sort="Tsybovsky, Yaroslav" uniqKey="Tsybovsky Y" first="Yaroslav" last="Tsybovsky">Yaroslav Tsybovsky</name><affiliation><nlm:aff id="A7">Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Thomas, Paul V" sort="Thomas, Paul V" uniqKey="Thomas P" first="Paul V" last="Thomas">Paul V. Thomas</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Druz, Aliaksandr" sort="Druz, Aliaksandr" uniqKey="Druz A" first="Aliaksandr" last="Druz">Aliaksandr Druz</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Zhang, Baoshan" sort="Zhang, Baoshan" uniqKey="Zhang B" first="Baoshan" last="Zhang">Baoshan Zhang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Park, Daechan" sort="Park, Daechan" uniqKey="Park D" first="Daechan" last="Park">Daechan Park</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Popova, Lyubov I" sort="Popova, Lyubov I" uniqKey="Popova L" first="Lyubov I" last="Popova">Lyubov I. Popova</name><affiliation><nlm:aff id="A8">Department of Medicine, Section of Rheumatology, Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Dekker, Cornelia L" sort="Dekker, Cornelia L" uniqKey="Dekker C" first="Cornelia L" last="Dekker">Cornelia L. Dekker</name><affiliation><nlm:aff id="A9">Department of Pediatrics, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Davis, Mark M" sort="Davis, Mark M" uniqKey="Davis M" first="Mark M" last="Davis">Mark M. Davis</name><affiliation><nlm:aff id="A10">Howard Hughes Medical Institute, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A11">Department of Microbiology and Immunology, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Carter, Chalise E" sort="Carter, Chalise E" uniqKey="Carter C" first="Chalise E" last="Carter">Chalise E. Carter</name><affiliation><nlm:aff id="A12">Center for Vaccines and Immunology, Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA</nlm:aff></affiliation></author><author><name sortKey="Ross, Ted M" sort="Ross, Ted M" uniqKey="Ross T" first="Ted M" last="Ross">Ted M. Ross</name><affiliation><nlm:aff id="A12">Center for Vaccines and Immunology, Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA</nlm:aff></affiliation></author><author><name sortKey="Ellington, Andrew D" sort="Ellington, Andrew D" uniqKey="Ellington A" first="Andrew D" last="Ellington">Andrew D. Ellington</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Wilson, Patrick C" sort="Wilson, Patrick C" uniqKey="Wilson P" first="Patrick C" last="Wilson">Patrick C. Wilson</name><affiliation><nlm:aff id="A8">Department of Medicine, Section of Rheumatology, Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Marcotte, Edward M" sort="Marcotte, Edward M" uniqKey="Marcotte E" first="Edward M" last="Marcotte">Edward M. Marcotte</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R" last="Mascola">John R. Mascola</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Ippolito, Gregory C" sort="Ippolito, Gregory C" uniqKey="Ippolito G" first="Gregory C" last="Ippolito">Gregory C. Ippolito</name><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Krammer, Florian" sort="Krammer, Florian" uniqKey="Krammer F" first="Florian" last="Krammer">Florian Krammer</name><affiliation><nlm:aff id="A3">Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</nlm:aff></affiliation></author><author><name sortKey="Quake, Stephen R" sort="Quake, Stephen R" uniqKey="Quake S" first="Stephen R" last="Quake">Stephen R. Quake</name><affiliation><nlm:aff id="A5">Department of Bioengineering, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A10">Howard Hughes Medical Institute, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A14">Department of Applied Physics, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D" last="Kwong">Peter D. Kwong</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Georgiou, George" sort="Georgiou, George" uniqKey="Georgiou G" first="George" last="Georgiou">George Georgiou</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A6">Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27820605</idno><idno type="pmc">5301914</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301914</idno><idno type="RBID">PMC:5301914</idno><idno type="doi">10.1038/nm.4224</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000885</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000885</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination</title><author><name sortKey="Lee, Jiwon" sort="Lee, Jiwon" uniqKey="Lee J" first="Jiwon" last="Lee">Jiwon Lee</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Boutz, Daniel R" sort="Boutz, Daniel R" uniqKey="Boutz D" first="Daniel R" last="Boutz">Daniel R. Boutz</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Chromikova, Veronika" sort="Chromikova, Veronika" uniqKey="Chromikova V" first="Veronika" last="Chromikova">Veronika Chromikova</name><affiliation><nlm:aff id="A3">Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</nlm:aff></affiliation></author><author><name sortKey="Joyce, M Gordon" sort="Joyce, M Gordon" uniqKey="Joyce M" first="M Gordon" last="Joyce">M Gordon Joyce</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Vollmers, Christopher" sort="Vollmers, Christopher" uniqKey="Vollmers C" first="Christopher" last="Vollmers">Christopher Vollmers</name><affiliation><nlm:aff id="A5">Department of Bioengineering, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Horton, Andrew P" sort="Horton, Andrew P" uniqKey="Horton A" first="Andrew P" last="Horton">Andrew P. Horton</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Dekosky, Brandon J" sort="Dekosky, Brandon J" uniqKey="Dekosky B" first="Brandon J" last="Dekosky">Brandon J. Dekosky</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Lee, Chang Han" sort="Lee, Chang Han" uniqKey="Lee C" first="Chang-Han" last="Lee">Chang-Han Lee</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Lavinder, Jason J" sort="Lavinder, Jason J" uniqKey="Lavinder J" first="Jason J" last="Lavinder">Jason J. Lavinder</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Murrin, Ellen M" sort="Murrin, Ellen M" uniqKey="Murrin E" first="Ellen M" last="Murrin">Ellen M. Murrin</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Chrysostomou, Constantine" sort="Chrysostomou, Constantine" uniqKey="Chrysostomou C" first="Constantine" last="Chrysostomou">Constantine Chrysostomou</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Hoi, Kam Hon" sort="Hoi, Kam Hon" uniqKey="Hoi K" first="Kam Hon" last="Hoi">Kam Hon Hoi</name><affiliation><nlm:aff id="A6">Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Tsybovsky, Yaroslav" sort="Tsybovsky, Yaroslav" uniqKey="Tsybovsky Y" first="Yaroslav" last="Tsybovsky">Yaroslav Tsybovsky</name><affiliation><nlm:aff id="A7">Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Thomas, Paul V" sort="Thomas, Paul V" uniqKey="Thomas P" first="Paul V" last="Thomas">Paul V. Thomas</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Druz, Aliaksandr" sort="Druz, Aliaksandr" uniqKey="Druz A" first="Aliaksandr" last="Druz">Aliaksandr Druz</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Zhang, Baoshan" sort="Zhang, Baoshan" uniqKey="Zhang B" first="Baoshan" last="Zhang">Baoshan Zhang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Park, Daechan" sort="Park, Daechan" uniqKey="Park D" first="Daechan" last="Park">Daechan Park</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Popova, Lyubov I" sort="Popova, Lyubov I" uniqKey="Popova L" first="Lyubov I" last="Popova">Lyubov I. Popova</name><affiliation><nlm:aff id="A8">Department of Medicine, Section of Rheumatology, Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Dekker, Cornelia L" sort="Dekker, Cornelia L" uniqKey="Dekker C" first="Cornelia L" last="Dekker">Cornelia L. Dekker</name><affiliation><nlm:aff id="A9">Department of Pediatrics, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Davis, Mark M" sort="Davis, Mark M" uniqKey="Davis M" first="Mark M" last="Davis">Mark M. Davis</name><affiliation><nlm:aff id="A10">Howard Hughes Medical Institute, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A11">Department of Microbiology and Immunology, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Carter, Chalise E" sort="Carter, Chalise E" uniqKey="Carter C" first="Chalise E" last="Carter">Chalise E. Carter</name><affiliation><nlm:aff id="A12">Center for Vaccines and Immunology, Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA</nlm:aff></affiliation></author><author><name sortKey="Ross, Ted M" sort="Ross, Ted M" uniqKey="Ross T" first="Ted M" last="Ross">Ted M. Ross</name><affiliation><nlm:aff id="A12">Center for Vaccines and Immunology, Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA</nlm:aff></affiliation></author><author><name sortKey="Ellington, Andrew D" sort="Ellington, Andrew D" uniqKey="Ellington A" first="Andrew D" last="Ellington">Andrew D. Ellington</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Wilson, Patrick C" sort="Wilson, Patrick C" uniqKey="Wilson P" first="Patrick C" last="Wilson">Patrick C. Wilson</name><affiliation><nlm:aff id="A8">Department of Medicine, Section of Rheumatology, Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Marcotte, Edward M" sort="Marcotte, Edward M" uniqKey="Marcotte E" first="Edward M" last="Marcotte">Edward M. Marcotte</name><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R" last="Mascola">John R. Mascola</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Ippolito, Gregory C" sort="Ippolito, Gregory C" uniqKey="Ippolito G" first="Gregory C" last="Ippolito">Gregory C. Ippolito</name><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author><author><name sortKey="Krammer, Florian" sort="Krammer, Florian" uniqKey="Krammer F" first="Florian" last="Krammer">Florian Krammer</name><affiliation><nlm:aff id="A3">Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</nlm:aff></affiliation></author><author><name sortKey="Quake, Stephen R" sort="Quake, Stephen R" uniqKey="Quake S" first="Stephen R" last="Quake">Stephen R. Quake</name><affiliation><nlm:aff id="A5">Department of Bioengineering, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A10">Howard Hughes Medical Institute, Stanford University, Stanford, California, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A14">Department of Applied Physics, Stanford University, Stanford, California, USA</nlm:aff></affiliation></author><author><name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D" last="Kwong">Peter D. Kwong</name><affiliation><nlm:aff id="A4">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</nlm:aff></affiliation></author><author><name sortKey="Georgiou, George" sort="Georgiou, George" uniqKey="Georgiou G" first="George" last="Georgiou">George Georgiou</name><affiliation><nlm:aff id="A1">Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A6">Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A13">Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</nlm:aff></affiliation></author></analytic><series><title level="j">Nature medicine</title><idno type="ISSN">1078-8956</idno><idno type="eISSN">1546-170X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Molecular understanding of serological immunity to influenza has been confounded by the complexity of the polyclonal antibody response in humans. Here we used high-resolution proteomics analysis of immunoglobulin (referred to as Ig-seq) coupled with high-throughput sequencing of transcripts encoding B cell receptors (BCR-seq) to quantitatively determine the antibody repertoire at the individual clonotype level in the sera of young adults before and after vaccination with trivalent seasonal influenza vaccine. The serum repertoire comprised between 40 and 147 clonotypes that were specific to each of the three monovalent components of the trivalent influenza vaccine, with boosted pre-existing clonotypes accounting for ~60% of the response. An unexpectedly high fraction of serum antibodies recognized both the H1 and H3 monovalent vaccines. Recombinant versions of these H1 + H3 cross-reactive antibodies showed broad binding to hemagglutinins (HAs) from previously circulating virus strains; several of these antibodies, which were prevalent in the serum of multiple donors, recognized the same conserved epitope in the HA head domain. Although the HA-head-specific H1 + H3 antibodies did not show neutralization activity <italic>in vitro</italic>, they protected mice against infection with the H1N1 and H3N2 virus strains when administered before or after challenge. Collectively, our data reveal unanticipated insights regarding the serological response to influenza vaccination and raise questions about the added benefits of using a quadrivalent vaccine instead of a trivalent vaccine.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9502015</journal-id><journal-id journal-id-type="pubmed-jr-id">8791</journal-id><journal-id journal-id-type="nlm-ta">Nat Med</journal-id><journal-id journal-id-type="iso-abbrev">Nat. Med.</journal-id><journal-title-group><journal-title>Nature medicine</journal-title></journal-title-group><issn pub-type="ppub">1078-8956</issn><issn pub-type="epub">1546-170X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">27820605</article-id><article-id pub-id-type="pmc">5301914</article-id><article-id pub-id-type="doi">10.1038/nm.4224</article-id><article-id pub-id-type="manuscript">NIHMS846495</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Jiwon</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Boutz</surname><given-names>Daniel R</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Chromikova</surname><given-names>Veronika</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Joyce</surname><given-names>M Gordon</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Vollmers</surname><given-names>Christopher</given-names></name><xref ref-type="aff" rid="A5">5</xref></contrib><contrib contrib-type="author"><name><surname>Leung</surname><given-names>Kwanyee</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Horton</surname><given-names>Andrew P</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>DeKosky</surname><given-names>Brandon J</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN2" ref-type="author-notes">15</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Chang-Han</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lavinder</surname><given-names>Jason J</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Murrin</surname><given-names>Ellen M</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chrysostomou</surname><given-names>Constantine</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hoi</surname><given-names>Kam Hon</given-names></name><xref ref-type="aff" rid="A6">6</xref><xref rid="FN2" ref-type="author-notes">15</xref></contrib><contrib contrib-type="author"><name><surname>Tsybovsky</surname><given-names>Yaroslav</given-names></name><xref ref-type="aff" rid="A7">7</xref></contrib><contrib contrib-type="author"><name><surname>Thomas</surname><given-names>Paul V</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Druz</surname><given-names>Aliaksandr</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Baoshan</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Lingshu</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Kong</surname><given-names>Wing-Pui</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>Daechan</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Popova</surname><given-names>Lyubov I</given-names></name><xref ref-type="aff" rid="A8">8</xref><xref rid="FN2" ref-type="author-notes">15</xref></contrib><contrib contrib-type="author"><name><surname>Dekker</surname><given-names>Cornelia L</given-names></name><xref ref-type="aff" rid="A9">9</xref></contrib><contrib contrib-type="author"><name><surname>Davis</surname><given-names>Mark M</given-names></name><xref ref-type="aff" rid="A10">10</xref><xref ref-type="aff" rid="A11">11</xref></contrib><contrib contrib-type="author"><name><surname>Carter</surname><given-names>Chalise E</given-names></name><xref ref-type="aff" rid="A12">12</xref></contrib><contrib contrib-type="author"><name><surname>Ross</surname><given-names>Ted M</given-names></name><xref ref-type="aff" rid="A12">12</xref></contrib><contrib contrib-type="author"><name><surname>Ellington</surname><given-names>Andrew D</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A13">13</xref></contrib><contrib contrib-type="author"><name><surname>Wilson</surname><given-names>Patrick C</given-names></name><xref ref-type="aff" rid="A8">8</xref></contrib><contrib contrib-type="author"><name><surname>Marcotte</surname><given-names>Edward M</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A13">13</xref></contrib><contrib contrib-type="author"><name><surname>Mascola</surname><given-names>John R</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Ippolito</surname><given-names>Gregory C</given-names></name><xref ref-type="aff" rid="A13">13</xref></contrib><contrib contrib-type="author"><name><surname>Krammer</surname><given-names>Florian</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Quake</surname><given-names>Stephen R</given-names></name><xref ref-type="aff" rid="A5">5</xref><xref ref-type="aff" rid="A10">10</xref><xref ref-type="aff" rid="A14">14</xref></contrib><contrib contrib-type="author"><name><surname>Kwong</surname><given-names>Peter D</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Georgiou</surname><given-names>George</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A6">6</xref><xref ref-type="aff" rid="A13">13</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA</aff><aff id="A2"><label>2</label>Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA</aff><aff id="A3"><label>3</label>Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</aff><aff id="A4"><label>4</label>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA</aff><aff id="A5"><label>5</label>Department of Bioengineering, Stanford University, Stanford, California, USA</aff><aff id="A6"><label>6</label>Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA</aff><aff id="A7"><label>7</label>Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA</aff><aff id="A8"><label>8</label>Department of Medicine, Section of Rheumatology, Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois, USA</aff><aff id="A9"><label>9</label>Department of Pediatrics, Stanford University, Stanford, California, USA</aff><aff id="A10"><label>10</label>Howard Hughes Medical Institute, Stanford University, Stanford, California, USA</aff><aff id="A11"><label>11</label>Department of Microbiology and Immunology, Stanford University, Stanford, California, USA</aff><aff id="A12"><label>12</label>Center for Vaccines and Immunology, Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA</aff><aff id="A13"><label>13</label>Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA</aff><aff id="A14"><label>14</label>Department of Applied Physics, Stanford University, Stanford, California, USA</aff><author-notes><corresp id="FN1">Correspondence should be addressed to G.G. (<email>gg@che.utexas.edu</email>)</corresp><fn id="FN2" fn-type="present-address"><label>15</label><p>Present addresses: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA (B.J.D.); Antibody Engineering Department, Genentech, Inc., South San Francisco, California, USA (K.H.H.); Division of Infectious Diseases, SRI International, Harrisonburg, Virginia, USA (L.I.P.).</p></fn><fn fn-type="COI-statement" id="FN6"><p><bold>COMPETING FINANCIAL INTERESTS</bold></p><p>The authors declare no competing financial interests.</p></fn></author-notes><pub-date pub-type="nihms-submitted"><day>1</day><month>2</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>07</day><month>11</month><year>2016</year></pub-date><pub-date pub-type="ppub"><month>12</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>10</day><month>2</month><year>2017</year></pub-date><volume>22</volume><issue>12</issue><fpage>1456</fpage><lpage>1464</lpage><pmc-comment>elocation-id from pubmed: 10.1038/nm.4224</pmc-comment>
<permissions><license license-type="permissions-link"><license-p>Reprints and permissions information is available online at <ext-link ext-link-type="uri" xlink:href="http://www.nature.com/reprints/index.html">http://www.nature.com/reprints/index.html</ext-link>.</license-p></license></permissions><abstract><p id="P1">Molecular understanding of serological immunity to influenza has been confounded by the complexity of the polyclonal antibody response in humans. Here we used high-resolution proteomics analysis of immunoglobulin (referred to as Ig-seq) coupled with high-throughput sequencing of transcripts encoding B cell receptors (BCR-seq) to quantitatively determine the antibody repertoire at the individual clonotype level in the sera of young adults before and after vaccination with trivalent seasonal influenza vaccine. The serum repertoire comprised between 40 and 147 clonotypes that were specific to each of the three monovalent components of the trivalent influenza vaccine, with boosted pre-existing clonotypes accounting for ~60% of the response. An unexpectedly high fraction of serum antibodies recognized both the H1 and H3 monovalent vaccines. Recombinant versions of these H1 + H3 cross-reactive antibodies showed broad binding to hemagglutinins (HAs) from previously circulating virus strains; several of these antibodies, which were prevalent in the serum of multiple donors, recognized the same conserved epitope in the HA head domain. Although the HA-head-specific H1 + H3 antibodies did not show neutralization activity <italic>in vitro</italic>, they protected mice against infection with the H1N1 and H3N2 virus strains when administered before or after challenge. Collectively, our data reveal unanticipated insights regarding the serological response to influenza vaccination and raise questions about the added benefits of using a quadrivalent vaccine instead of a trivalent vaccine.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0008853 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0008853 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.33. |  |