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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection</title><author><name sortKey="Chandler, Darrell P" sort="Chandler, Darrell P" uniqKey="Chandler D" first="Darrell P." last="Chandler">Darrell P. Chandler</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Griesemer, Sara B" sort="Griesemer, Sara B" uniqKey="Griesemer S" first="Sara B." last="Griesemer">Sara B. Griesemer</name><affiliation><nlm:aff id="A2">Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany NY 12208</nlm:aff></affiliation></author><author><name sortKey="Knickerbocker, Christopher" sort="Knickerbocker, Christopher" uniqKey="Knickerbocker C" first="Christopher" last="Knickerbocker">Christopher Knickerbocker</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Golova, Julia B" sort="Golova, Julia B" uniqKey="Golova J" first="Julia B." last="Golova">Julia B. Golova</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Lambarqui, Amine" sort="Lambarqui, Amine" uniqKey="Lambarqui A" first="Amine" last="Lambarqui">Amine Lambarqui</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Perov, Alexander N" sort="Perov, Alexander N" uniqKey="Perov A" first="Alexander N." last="Perov">Alexander N. Perov</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Zimmerman, Cynthia" sort="Zimmerman, Cynthia" uniqKey="Zimmerman C" first="Cynthia" last="Zimmerman">Cynthia Zimmerman</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Wiles, Cory" sort="Wiles, Cory" uniqKey="Wiles C" first="Cory" last="Wiles">Cory Wiles</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Rudy, George B" sort="Rudy, George B" uniqKey="Rudy G" first="George B." last="Rudy">George B. Rudy</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="St George, Kirsten" sort="St George, Kirsten" uniqKey="St George K" first="Kirsten" last="St. George">Kirsten St. George</name><affiliation><nlm:aff id="A2">Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany NY 12208</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25066276</idno><idno type="pmc">4175443</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175443</idno><idno type="RBID">PMC:4175443</idno><idno type="doi">10.1016/j.jviromet.2014.07.019</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000854</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000854</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection</title><author><name sortKey="Chandler, Darrell P" sort="Chandler, Darrell P" uniqKey="Chandler D" first="Darrell P." last="Chandler">Darrell P. Chandler</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Griesemer, Sara B" sort="Griesemer, Sara B" uniqKey="Griesemer S" first="Sara B." last="Griesemer">Sara B. Griesemer</name><affiliation><nlm:aff id="A2">Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany NY 12208</nlm:aff></affiliation></author><author><name sortKey="Knickerbocker, Christopher" sort="Knickerbocker, Christopher" uniqKey="Knickerbocker C" first="Christopher" last="Knickerbocker">Christopher Knickerbocker</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Golova, Julia B" sort="Golova, Julia B" uniqKey="Golova J" first="Julia B." last="Golova">Julia B. Golova</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Lambarqui, Amine" sort="Lambarqui, Amine" uniqKey="Lambarqui A" first="Amine" last="Lambarqui">Amine Lambarqui</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Perov, Alexander N" sort="Perov, Alexander N" uniqKey="Perov A" first="Alexander N." last="Perov">Alexander N. Perov</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Zimmerman, Cynthia" sort="Zimmerman, Cynthia" uniqKey="Zimmerman C" first="Cynthia" last="Zimmerman">Cynthia Zimmerman</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Wiles, Cory" sort="Wiles, Cory" uniqKey="Wiles C" first="Cory" last="Wiles">Cory Wiles</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="Rudy, George B" sort="Rudy, George B" uniqKey="Rudy G" first="George B." last="Rudy">George B. Rudy</name><affiliation><nlm:aff id="A1">Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</nlm:aff></affiliation></author><author><name sortKey="St George, Kirsten" sort="St George, Kirsten" uniqKey="St George K" first="Kirsten" last="St. George">Kirsten St. George</name><affiliation><nlm:aff id="A2">Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany NY 12208</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">The objectives of this study were to develop a user-friendly, gel element microarray test for influenza virus detection, subtyping, and neuraminidase inhibitor resistance detection, assess the performance characteristics of the assay, and perform a clinical evaluation on retrospective nasopharyngeal swab specimens. A streamlined microarray workflow enabled a single user to run up to 24 tests in an 8 hour shift. The most sensitive components of the test were the primers and probes targeting the A/H1pdm09 HA gene with an analytical limit of detection (LoD) <100 gene copies (gc) per reaction. LoDs for all targets in nasopharyngeal swab samples were ≤ 1000 gc, with the exception of one target in the seasonal A/H1N1 subtype. Seasonal H275Y variants were detectable in a mixed population when present at > 5% with wild type virus, while the 2009 pandemic H1N1 H275Y variant was detectable at ≤1% in a mixture with pandemic wild type virus. Influenza typing and sub typing results concurred with those obtained with real-time RT-PCR assays on more than 97% of the samples tested. The results demonstrate that a large panel of single-plex, real-time RT-PCR tests can be translated to an easy-to-use, sensitive, and specific microarray test for potential diagnostic use.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8005839</journal-id><journal-id journal-id-type="pubmed-jr-id">4621</journal-id><journal-id journal-id-type="nlm-ta">J Virol Methods</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol. Methods</journal-id><journal-title-group><journal-title>Journal of virological methods</journal-title></journal-title-group><issn pub-type="ppub">0166-0934</issn><issn pub-type="epub">1879-0984</issn></journal-meta><article-meta><article-id pub-id-type="pmid">25066276</article-id><article-id pub-id-type="pmc">4175443</article-id><article-id pub-id-type="doi">10.1016/j.jviromet.2014.07.019</article-id><article-id pub-id-type="manuscript">NIHMS626715</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chandler</surname><given-names>Darrell P.</given-names></name><xref rid="FN1" ref-type="author-notes">*</xref><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Griesemer</surname><given-names>Sara B.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Knickerbocker</surname><given-names>Christopher</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Golova</surname><given-names>Julia B.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lambarqui</surname><given-names>Amine</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Perov</surname><given-names>Alexander N.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zimmerman</surname><given-names>Cynthia</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wiles</surname><given-names>Cory</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Rudy</surname><given-names>George B.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>St. George</surname><given-names>Kirsten</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib></contrib-group><aff id="A1"><label>1</label>Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick MD 21701</aff><aff id="A2"><label>2</label>Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany NY 12208</aff><author-notes><corresp id="FN1"><label>*</label>Corresponding author: Tel: 734-428-0713, Fax: 301-698-0202, <email>dchandler@akonni.com</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>9</day><month>9</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>24</day><month>7</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>11</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>11</month><year>2015</year></pub-date><volume>208</volume><fpage>152</fpage><lpage>159</lpage><pmc-comment>elocation-id from pubmed: 10.1016/j.jviromet.2014.07.019</pmc-comment>
<permissions><copyright-statement>© 2014 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><p id="P2">The objectives of this study were to develop a user-friendly, gel element microarray test for influenza virus detection, subtyping, and neuraminidase inhibitor resistance detection, assess the performance characteristics of the assay, and perform a clinical evaluation on retrospective nasopharyngeal swab specimens. A streamlined microarray workflow enabled a single user to run up to 24 tests in an 8 hour shift. The most sensitive components of the test were the primers and probes targeting the A/H1pdm09 HA gene with an analytical limit of detection (LoD) <100 gene copies (gc) per reaction. LoDs for all targets in nasopharyngeal swab samples were ≤ 1000 gc, with the exception of one target in the seasonal A/H1N1 subtype. Seasonal H275Y variants were detectable in a mixed population when present at > 5% with wild type virus, while the 2009 pandemic H1N1 H275Y variant was detectable at ≤1% in a mixture with pandemic wild type virus. Influenza typing and sub typing results concurred with those obtained with real-time RT-PCR assays on more than 97% of the samples tested. The results demonstrate that a large panel of single-plex, real-time RT-PCR tests can be translated to an easy-to-use, sensitive, and specific microarray test for potential diagnostic use.</p></abstract><kwd-group><kwd>molecular diagnostic</kwd><kwd>microarray</kwd><kwd>oseltamivir reisistance</kwd><kwd>surveillance</kwd><kwd>epidemiology</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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