Replication-competent influenza A viruses expressing a red fluorescent protein
Identifieur interne : 000786 ( Pmc/Corpus ); précédent : 000785; suivant : 000787Replication-competent influenza A viruses expressing a red fluorescent protein
Auteurs : Aitor Nogales ; Steven F. Baker ; Luis Martínez-SobridoSource :
- Virology [ 0042-6822 ] ; 2014.
Abstract
Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses
Url:
DOI: 10.1016/j.virol.2014.12.006
PubMed: 25553516
PubMed Central: 4323957
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PMC:4323957Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Replication-competent influenza A viruses expressing a red fluorescent protein</title><author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name></author><author><name sortKey="Baker, Steven F" sort="Baker, Steven F" uniqKey="Baker S" first="Steven F." last="Baker">Steven F. Baker</name></author><author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25553516</idno><idno type="pmc">4323957</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323957</idno><idno type="RBID">PMC:4323957</idno><idno type="doi">10.1016/j.virol.2014.12.006</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000786</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000786</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Replication-competent influenza A viruses expressing a red fluorescent protein</title><author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name></author><author><name sortKey="Baker, Steven F" sort="Baker, Steven F" uniqKey="Baker S" first="Steven F." last="Baker">Steven F. Baker</name></author><author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><idno type="eISSN">1096-0341</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses <italic>in vitro</italic>. Infection of cells with influenza A mCherry viruses was neutralized with monoclonal antibodies and inhibited with antivirals to levels similar to wild-type virus. Influenza A mCherry viruses were also able to lethally infect mice, and strikingly, dose- and time-dependent kinetics of viral replication were monitored in whole excised mouse lungs using an <italic>in vivo</italic> imaging system (IVIS). By eliminating the need for secondary labeling of infected cells, influenza A mCherry viruses provide an ideal tool in the ongoing struggle to better characterize the virus and identify new therapeutics against influenza A viral infections.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0110674</journal-id><journal-id journal-id-type="pubmed-jr-id">8015</journal-id><journal-id journal-id-type="nlm-ta">Virology</journal-id><journal-id journal-id-type="iso-abbrev">Virology</journal-id><journal-title-group><journal-title>Virology</journal-title></journal-title-group><issn pub-type="ppub">0042-6822</issn><issn pub-type="epub">1096-0341</issn></journal-meta><article-meta><article-id pub-id-type="pmid">25553516</article-id><article-id pub-id-type="pmc">4323957</article-id><article-id pub-id-type="doi">10.1016/j.virol.2014.12.006</article-id><article-id pub-id-type="manuscript">NIHMS648687</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Replication-competent influenza A viruses expressing a red fluorescent protein</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nogales</surname><given-names>Aitor</given-names></name><xref rid="FN2" ref-type="author-notes">†</xref></contrib><contrib contrib-type="author"><name><surname>Baker</surname><given-names>Steven F.</given-names></name><xref rid="FN2" ref-type="author-notes">†</xref></contrib><contrib contrib-type="author"><name><surname>Martínez-Sobrido</surname><given-names>Luis</given-names></name><xref rid="FN1" ref-type="author-notes">*</xref></contrib><aff id="A1">Department of Microbiology and Immunology, University of Rochester, Rochester, NY</aff></contrib-group><author-notes><corresp id="FN1"><label>*</label>To whom correspondence should be addressed: Luis Martínez-Sobrido, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, Tel.: (585) 276-4733, <email>luis_martinez@urmc.rochester.edu</email></corresp><fn id="FN2" fn-type="equal"><label>†</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="nihms-submitted"><day>14</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>2</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>2</month><year>2016</year></pub-date><volume>476</volume><fpage>206</fpage><lpage>216</lpage><pmc-comment>elocation-id from pubmed: 10.1016/j.virol.2014.12.006</pmc-comment>
<permissions><copyright-statement>© 2014 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><p id="P1">Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses <italic>in vitro</italic>. Infection of cells with influenza A mCherry viruses was neutralized with monoclonal antibodies and inhibited with antivirals to levels similar to wild-type virus. Influenza A mCherry viruses were also able to lethally infect mice, and strikingly, dose- and time-dependent kinetics of viral replication were monitored in whole excised mouse lungs using an <italic>in vivo</italic> imaging system (IVIS). By eliminating the need for secondary labeling of infected cells, influenza A mCherry viruses provide an ideal tool in the ongoing struggle to better characterize the virus and identify new therapeutics against influenza A viral infections.</p></abstract><kwd-group><kwd>Influenza A virus</kwd><kwd>NS1</kwd><kwd>mCherry</kwd><kwd>replication-competent virus</kwd><kwd>2A</kwd><kwd>neutralizing antibodies</kwd><kwd>microneutralization assay</kwd><kwd>virus neutralization assay</kwd><kwd>antivirals</kwd><kwd>interferon</kwd><kwd><italic>in vivo</italic> imaging system (IVIS)</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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