Replication-competent influenza A viruses expressing a red fluorescent protein
Identifieur interne : 000786 ( Pmc/Corpus ); précédent : 000785; suivant : 000787Replication-competent influenza A viruses expressing a red fluorescent protein
Auteurs : Aitor Nogales ; Steven F. Baker ; Luis Martínez-SobridoSource :
- Virology [ 0042-6822 ] ; 2014.
Abstract
Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses
Url:
DOI: 10.1016/j.virol.2014.12.006
PubMed: 25553516
PubMed Central: 4323957
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PMC:4323957Le document en format XML
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<author><name sortKey="Baker, Steven F" sort="Baker, Steven F" uniqKey="Baker S" first="Steven F." last="Baker">Steven F. Baker</name>
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<author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name>
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<author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name>
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<front><div type="abstract" xml:lang="en"><p id="P1">Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses <italic>in vitro</italic>
. Infection of cells with influenza A mCherry viruses was neutralized with monoclonal antibodies and inhibited with antivirals to levels similar to wild-type virus. Influenza A mCherry viruses were also able to lethally infect mice, and strikingly, dose- and time-dependent kinetics of viral replication were monitored in whole excised mouse lungs using an <italic>in vivo</italic>
imaging system (IVIS). By eliminating the need for secondary labeling of infected cells, influenza A mCherry viruses provide an ideal tool in the ongoing struggle to better characterize the virus and identify new therapeutics against influenza A viral infections.</p>
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<contrib-group><contrib contrib-type="author"><name><surname>Nogales</surname>
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<xref rid="FN2" ref-type="author-notes">†</xref>
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<contrib contrib-type="author"><name><surname>Baker</surname>
<given-names>Steven F.</given-names>
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<xref rid="FN2" ref-type="author-notes">†</xref>
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<contrib contrib-type="author"><name><surname>Martínez-Sobrido</surname>
<given-names>Luis</given-names>
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<xref rid="FN1" ref-type="author-notes">*</xref>
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<aff id="A1">Department of Microbiology and Immunology, University of Rochester, Rochester, NY</aff>
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<author-notes><corresp id="FN1"><label>*</label>
To whom correspondence should be addressed: Luis Martínez-Sobrido, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, Tel.: (585) 276-4733, <email>luis_martinez@urmc.rochester.edu</email>
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<fn id="FN2" fn-type="equal"><label>†</label>
<p>These authors contributed equally to this work.</p>
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<pub-date pub-type="nihms-submitted"><day>14</day>
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<volume>476</volume>
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<lpage>216</lpage>
<pmc-comment>elocation-id from pubmed: 10.1016/j.virol.2014.12.006</pmc-comment>
<permissions><copyright-statement>© 2014 Elsevier Inc. All rights reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
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<abstract><p id="P1">Like most animal viruses, studying influenza A in model systems requires secondary methodologies to identify infected cells. To circumvent this requirement, we describe the generation of replication-competent influenza A red fluorescent viruses. These influenza A viruses encode mCherry fused to the viral non-structural 1 (NS1) protein and display comparable growth kinetics to wild-type viruses <italic>in vitro</italic>
. Infection of cells with influenza A mCherry viruses was neutralized with monoclonal antibodies and inhibited with antivirals to levels similar to wild-type virus. Influenza A mCherry viruses were also able to lethally infect mice, and strikingly, dose- and time-dependent kinetics of viral replication were monitored in whole excised mouse lungs using an <italic>in vivo</italic>
imaging system (IVIS). By eliminating the need for secondary labeling of infected cells, influenza A mCherry viruses provide an ideal tool in the ongoing struggle to better characterize the virus and identify new therapeutics against influenza A viral infections.</p>
</abstract>
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