H2N2V1, Pmc, Corpus, bibRecord, 0006880

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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assessment of the Internal Genes of Influenza A (H7N9) Virus Contributing to High Pathogenicity in Mice</title>
<author><name sortKey="Bi, Yuhai" sort="Bi, Yuhai" uniqKey="Bi Y" first="Yuhai" last="Bi">Yuhai Bi</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xie, Qing" sort="Xie, Qing" uniqKey="Xie Q" first="Qing" last="Xie">Qing Xie</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff8">Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Shuang" sort="Zhang, Shuang" uniqKey="Zhang S" first="Shuang" last="Zhang">Shuang Zhang</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yun" sort="Li, Yun" uniqKey="Li Y" first="Yun" last="Li">Yun Li</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xiao, Haixia" sort="Xiao, Haixia" uniqKey="Xiao H" first="Haixia" last="Xiao">Haixia Xiao</name>
<affiliation><nlm:aff id="aff2">Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jin, Tao" sort="Jin, Tao" uniqKey="Jin T" first="Tao" last="Jin">Tao Jin</name>
<affiliation><nlm:aff id="aff7">BGI-Shenzhen, Shenzhen, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zheng, Weinan" sort="Zheng, Weinan" uniqKey="Zheng W" first="Weinan" last="Zheng">Weinan Zheng</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jia, Xiaojuan" sort="Jia, Xiaojuan" uniqKey="Jia X" first="Xiaojuan" last="Jia">Xiaojuan Jia</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sun, Lei" sort="Sun, Lei" uniqKey="Sun L" first="Lei" last="Sun">Lei Sun</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Jinhua" sort="Liu, Jinhua" uniqKey="Liu J" first="Jinhua" last="Liu">Jinhua Liu</name>
<affiliation><nlm:aff id="aff3">Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name>
<affiliation><nlm:aff id="aff4">Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff5">Office of Director General, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff6">Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Hanzhou, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Wenjun" sort="Liu, Wenjun" uniqKey="Liu W" first="Wenjun" last="Liu">Wenjun Liu</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25320305</idno>
<idno type="pmc">4301103</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301103</idno>
<idno type="RBID">PMC:4301103</idno>
<idno type="doi">10.1128/JVI.02390-14</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000688</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000688</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Assessment of the Internal Genes of Influenza A (H7N9) Virus Contributing to High Pathogenicity in Mice</title>
<author><name sortKey="Bi, Yuhai" sort="Bi, Yuhai" uniqKey="Bi Y" first="Yuhai" last="Bi">Yuhai Bi</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xie, Qing" sort="Xie, Qing" uniqKey="Xie Q" first="Qing" last="Xie">Qing Xie</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff8">Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Shuang" sort="Zhang, Shuang" uniqKey="Zhang S" first="Shuang" last="Zhang">Shuang Zhang</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yun" sort="Li, Yun" uniqKey="Li Y" first="Yun" last="Li">Yun Li</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xiao, Haixia" sort="Xiao, Haixia" uniqKey="Xiao H" first="Haixia" last="Xiao">Haixia Xiao</name>
<affiliation><nlm:aff id="aff2">Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jin, Tao" sort="Jin, Tao" uniqKey="Jin T" first="Tao" last="Jin">Tao Jin</name>
<affiliation><nlm:aff id="aff7">BGI-Shenzhen, Shenzhen, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zheng, Weinan" sort="Zheng, Weinan" uniqKey="Zheng W" first="Weinan" last="Zheng">Weinan Zheng</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jia, Xiaojuan" sort="Jia, Xiaojuan" uniqKey="Jia X" first="Xiaojuan" last="Jia">Xiaojuan Jia</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sun, Lei" sort="Sun, Lei" uniqKey="Sun L" first="Lei" last="Sun">Lei Sun</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Jinhua" sort="Liu, Jinhua" uniqKey="Liu J" first="Jinhua" last="Liu">Jinhua Liu</name>
<affiliation><nlm:aff id="aff3">Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name>
<affiliation><nlm:aff id="aff4">Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff5">Office of Director General, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff6">Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Hanzhou, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Wenjun" sort="Liu, Wenjun" uniqKey="Liu W" first="Wenjun" last="Liu">Wenjun Liu</name>
<affiliation><nlm:aff id="aff1">CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>The recently identified H7N9 influenza A virus has caused severe economic losses and worldwide public concern. Genetic analysis indicates that its six internal genes all originated from H9N2 viruses. However, the H7N9 virus is more highly pathogenic in humans than H9N2, which suggests that the internal genes of H7N9 have mutated. To analyze which H7N9 virus internal genes contribute to its high pathogenicity, a series of reassortants was generated by reverse genetics, with each virus containing a single internal gene of the typical A/Anhui/1/2013 (H7N9) (AH-H7N9) virus in the genetic background of the A/chicken/Shandong/lx1023/2007 (H9N2) virus. The replication ability, polymerase activity, and pathogenicity of these viruses were then evaluated <italic>in vitro</italic>
 and <italic>in vivo</italic>. These recombinants displayed high genetic compatibility, and the H7N9-derived PB2, M, and NP genes were identified as the virulence genes for the reassortants in mice. Further investigation confirmed that the PB2 K627 residue is critical for the high pathogenicity of the H7N9 virus and the reassortant containing the H7N9-derived PB2 segment (H9N2-AH/PB2). Notably, the H7N9-derived PB2 gene displayed greater compatibility with the H9N2 genome than that of H7N9, endowing the H9N2-AH/PB2 reassortant with greater viability and virulence than the parental H7N9 virus. In addition, the H7N9 virus, with the exception of the H9N2 reassortants, could effectively replicate in human A549 cells. Our results indicate that PB2, M, and NP are the key virulence genes, together with the surface hemagglutinin (HA) and neuraminidase (NA) proteins, contributing to the high infectivity of the H7N9 virus in humans.
</p>
<p><bold>IMPORTANCE</bold>
 To date, the novel H7N9 influenza A virus has caused 437 human infections, with approximately 30% mortality. Previous work has primarily focused on the two viral surface proteins, HA and NA, but the contribution of the six internal genes to the high pathogenicity of H7N9 has not been systematically studied. Here, the H9N2 virus was used as a genetic backbone to evaluate the virulence genes of H7N9 virus <italic>in vitro</italic>
 and <italic>in vivo</italic>
. Our data indicate that the PB2, M, and NP genes play important roles in viral infection in mice and, together with HA and NA, contribute to the high infectivity of the H7N9 virus in humans.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Virol</journal-id>
<journal-id journal-id-type="hwp">jvi</journal-id>
<journal-id journal-id-type="pmc">jvi</journal-id>
<journal-id journal-id-type="publisher-id">JVI</journal-id>
<journal-title-group><journal-title>Journal of Virology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25320305</article-id>
<article-id pub-id-type="pmc">4301103</article-id>
<article-id pub-id-type="publisher-id">02390-14</article-id>
<article-id pub-id-type="doi">10.1128/JVI.02390-14</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Pathogenesis and Immunity</subject>
</subj-group>
</article-categories>
<title-group><article-title>Assessment of the Internal Genes of Influenza A (H7N9) Virus Contributing to High Pathogenicity in Mice</article-title>
<alt-title alt-title-type="running-head">Virulence Genes of H7N9 Virus</alt-title>
<alt-title alt-title-type="short-authors">Bi et al.</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Bi</surname>
<given-names>Yuhai</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xie</surname>
<given-names>Qing</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff8"><sup>h</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Shuang</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Yun</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xiao</surname>
<given-names>Haixia</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jin</surname>
<given-names>Tao</given-names>
</name>
<xref ref-type="aff" rid="aff7"><sup>g</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zheng</surname>
<given-names>Weinan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Jing</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jia</surname>
<given-names>Xiaojuan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Lei</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Jinhua</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qin</surname>
<given-names>Chuan</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Gao</surname>
<given-names>George F.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff5"><sup>e</sup>
</xref>
<xref ref-type="aff" rid="aff6"><sup>f</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Liu</surname>
<given-names>Wenjun</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China</aff>
<aff id="aff2"><label>b</label>
Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China</aff>
<aff id="aff3"><label>c</label>
Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China</aff>
<aff id="aff4"><label>d</label>
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China</aff>
<aff id="aff5"><label>e</label>
Office of Director General, Chinese Center for Disease Control and Prevention, Beijing, China</aff>
<aff id="aff6"><label>f</label>
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Hanzhou, China</aff>
<aff id="aff7"><label>g</label>
BGI-Shenzhen, Shenzhen, China</aff>
<aff id="aff8"><label>h</label>
Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, China</aff>
</contrib-group>
<contrib-group><contrib contrib-type="editor"><name><surname>Lyles</surname>
<given-names>D. S.</given-names>
</name>
<role>Editor</role>
</contrib>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to George F. Gao, <email>Gaof@im.ac.cn</email>
, or Wenjun Liu, <email>liuwj@im.ac.cn</email>
.</corresp>
<fn fn-type="other"><p>C.Q., G.F.G., and W.L. are co-senior authors of the paper.</p>
</fn>
<fn fn-type="other"><p><bold>Citation</bold>
 Bi Y, Xie Q, Zhang S, Li Y, Xiao H, Jin T, Zheng W, Li J, Jia X, Sun L, Liu J, Qin C, Gao GF, Liu W. 2015. Assessment of the internal genes of influenza A (H7N9) virus contributing to high pathogenicity in mice. J Virol 89:2–13. doi:<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1128/JVI.02390-14">10.1128/JVI.02390-14</ext-link>
.</p>
</fn>
</author-notes>
<pub-date pub-type="epreprint"><day>15</day>
<month>10</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>16</day>
<month>12</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="collection"><day>1</day>
<month>1</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>16</day>
<month>12</month>
<year>2014</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the . </pmc-comment>
      <volume>89</volume>
<issue>1</issue>
<fpage>2</fpage>
<lpage>13</lpage>
<history><date date-type="received"><day>18</day>
<month>8</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>29</day>
<month>9</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2015</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
<license license-type="open-access"><license-p>The authors have paid a fee to allow immediate free access to this article.</license-p>
</license>
</permissions>
<self-uri content-type="pdf" xlink:href="zjv00115000002.pdf"></self-uri>
<abstract><title>ABSTRACT</title>
<p>The recently identified H7N9 influenza A virus has caused severe economic losses and worldwide public concern. Genetic analysis indicates that its six internal genes all originated from H9N2 viruses. However, the H7N9 virus is more highly pathogenic in humans than H9N2, which suggests that the internal genes of H7N9 have mutated. To analyze which H7N9 virus internal genes contribute to its high pathogenicity, a series of reassortants was generated by reverse genetics, with each virus containing a single internal gene of the typical A/Anhui/1/2013 (H7N9) (AH-H7N9) virus in the genetic background of the A/chicken/Shandong/lx1023/2007 (H9N2) virus. The replication ability, polymerase activity, and pathogenicity of these viruses were then evaluated <italic>in vitro</italic>
 and <italic>in vivo</italic>. These recombinants displayed high genetic compatibility, and the H7N9-derived PB2, M, and NP genes were identified as the virulence genes for the reassortants in mice. Further investigation confirmed that the PB2 K627 residue is critical for the high pathogenicity of the H7N9 virus and the reassortant containing the H7N9-derived PB2 segment (H9N2-AH/PB2). Notably, the H7N9-derived PB2 gene displayed greater compatibility with the H9N2 genome than that of H7N9, endowing the H9N2-AH/PB2 reassortant with greater viability and virulence than the parental H7N9 virus. In addition, the H7N9 virus, with the exception of the H9N2 reassortants, could effectively replicate in human A549 cells. Our results indicate that PB2, M, and NP are the key virulence genes, together with the surface hemagglutinin (HA) and neuraminidase (NA) proteins, contributing to the high infectivity of the H7N9 virus in humans.
</p>
<p><bold>IMPORTANCE</bold>
 To date, the novel H7N9 influenza A virus has caused 437 human infections, with approximately 30% mortality. Previous work has primarily focused on the two viral surface proteins, HA and NA, but the contribution of the six internal genes to the high pathogenicity of H7N9 has not been systematically studied. Here, the H9N2 virus was used as a genetic backbone to evaluate the virulence genes of H7N9 virus <italic>in vitro</italic>
 and <italic>in vivo</italic>
. Our data indicate that the PB2, M, and NP genes play important roles in viral infection in mice and, together with HA and NA, contribute to the high infectivity of the H7N9 virus in humans.</p>
</abstract>
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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0006880 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0006880 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Pmc
   |étape=   Corpus
   |type=    RBID
   |clé=     
   |texte=   
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration H2N2

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri