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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In Vivo</italic>
Selection of H1N2 Influenza Virus Reassortants in the Ferret Model</title>
<author><name sortKey="Angel, Matthew" sort="Angel, Matthew" uniqKey="Angel M" first="Matthew" last="Angel">Matthew Angel</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kimble, J Brian" sort="Kimble, J Brian" uniqKey="Kimble J" first="J. Brian" last="Kimble">J. Brian Kimble</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name>
<affiliation><nlm:aff id="aff2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23302886</idno>
<idno type="pmc">3592131</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592131</idno>
<idno type="RBID">PMC:3592131</idno>
<idno type="doi">10.1128/JVI.02591-12</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000662</idno>
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</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In Vivo</italic>
Selection of H1N2 Influenza Virus Reassortants in the Ferret Model</title>
<author><name sortKey="Angel, Matthew" sort="Angel, Matthew" uniqKey="Angel M" first="Matthew" last="Angel">Matthew Angel</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kimble, J Brian" sort="Kimble, J Brian" uniqKey="Kimble J" first="J. Brian" last="Kimble">J. Brian Kimble</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name>
<affiliation><nlm:aff id="aff2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name>
<affiliation><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Although the ferret model has been extensively used to study pathogenesis and transmission of influenza viruses, little has been done to determine whether ferrets are a good surrogate animal model to study influenza virus reassortment. It has been previously shown that the pandemic 2009 H1N1 (H1N1pdm) virus was able to transmit efficiently in ferrets. In coinfection studies with either seasonal H1N1 or H3N2 strains (H1N1s or H3N2s, respectively), the H1N1pdm virus was able to outcompete these strains and become the dominant transmissible virus. However, lack of reassortment could have been the result of differences in the cell or tissue tropism of these viruses in the ferret. To address this issue, we performed coinfection studies with recombinant influenza viruses carrying the surface genes of a seasonal H3N2 strain in the background of an H1N1pdm strain and vice versa. After serial passages in ferrets, a dominant H1N2 virus population was obtained with a constellation of gene segments, most of which, except for the neuraminidase (NA) and PB1 segments, were from the H1N1pdm strain. Our studies suggest that ferrets recapitulate influenza virus reassortment events. The H1N2 virus generated through this process resembles similar viruses that are emerging in nature, particularly in pigs.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Virol</journal-id>
<journal-id journal-id-type="hwp">jvi</journal-id>
<journal-id journal-id-type="pmc">jvi</journal-id>
<journal-id journal-id-type="publisher-id">JVI</journal-id>
<journal-title-group><journal-title>Journal of Virology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23302886</article-id>
<article-id pub-id-type="pmc">3592131</article-id>
<article-id pub-id-type="publisher-id">02591-12</article-id>
<article-id pub-id-type="doi">10.1128/JVI.02591-12</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Genetic Diversity and Evolution</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>In Vivo</italic>
Selection of H1N2 Influenza Virus Reassortants in the Ferret Model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Angel</surname>
<given-names>Matthew</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kimble</surname>
<given-names>J. Brian</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pena</surname>
<given-names>Lindomar</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wan</surname>
<given-names>Hongquan</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Perez</surname>
<given-names>Daniel R.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</aff>
<aff id="aff2"><label>b</label>
Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</aff>
</contrib-group>
<author-notes><corresp>Address correspondence to Daniel R. Perez, <email>dperez1@umd.edu</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>3</month>
<year>2013</year>
</pub-date>
<volume>87</volume>
<issue>6</issue>
<fpage>3277</fpage>
<lpage>3283</lpage>
<history><date date-type="received"><day>20</day>
<month>9</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>23</day>
<month>12</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2013, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2013</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zjv00613003277.pdf"></self-uri>
<abstract><p>Although the ferret model has been extensively used to study pathogenesis and transmission of influenza viruses, little has been done to determine whether ferrets are a good surrogate animal model to study influenza virus reassortment. It has been previously shown that the pandemic 2009 H1N1 (H1N1pdm) virus was able to transmit efficiently in ferrets. In coinfection studies with either seasonal H1N1 or H3N2 strains (H1N1s or H3N2s, respectively), the H1N1pdm virus was able to outcompete these strains and become the dominant transmissible virus. However, lack of reassortment could have been the result of differences in the cell or tissue tropism of these viruses in the ferret. To address this issue, we performed coinfection studies with recombinant influenza viruses carrying the surface genes of a seasonal H3N2 strain in the background of an H1N1pdm strain and vice versa. After serial passages in ferrets, a dominant H1N2 virus population was obtained with a constellation of gene segments, most of which, except for the neuraminidase (NA) and PB1 segments, were from the H1N1pdm strain. Our studies suggest that ferrets recapitulate influenza virus reassortment events. The H1N2 virus generated through this process resembles similar viruses that are emerging in nature, particularly in pigs.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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