H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis
Identifieur interne : 000564 ( Pmc/Corpus ); précédent : 000563; suivant : 000565H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis
Auteurs : Quang Duy Trinh ; Yasuyuki Izumi ; Shihoko Komine-Aizawa ; Toshikatsu Shibata ; Yoshitaka Shimotai ; Kazumichi Kuroda ; Masashi Mizuguchi ; Hiroshi Ushijima ; Gil Mor ; Satoshi HayakawaSource :
- American journal of reproductive immunology (New York, N.Y. : 1989) [ 1046-7408 ] ; 2009.
Abstract
Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown.
The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)-G were also examined.
Intracellular localization of viral proteins was observed. Twenty-four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA-G was unchanged.
We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA-G expression and subsequent rejection by maternal immune system.
Url:
DOI: 10.1111/j.1600-0897.2009.00723.x
PubMed: 19694639
PubMed Central: 3025808
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis</title><author><name sortKey="Trinh, Quang Duy" sort="Trinh, Quang Duy" uniqKey="Trinh Q" first="Quang Duy" last="Trinh">Quang Duy Trinh</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation><affiliation><nlm:aff id="A2"> Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Izumi, Yasuyuki" sort="Izumi, Yasuyuki" uniqKey="Izumi Y" first="Yasuyuki" last="Izumi">Yasuyuki Izumi</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Komine Aizawa, Shihoko" sort="Komine Aizawa, Shihoko" uniqKey="Komine Aizawa S" first="Shihoko" last="Komine-Aizawa">Shihoko Komine-Aizawa</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Shibata, Toshikatsu" sort="Shibata, Toshikatsu" uniqKey="Shibata T" first="Toshikatsu" last="Shibata">Toshikatsu Shibata</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Kuroda, Kazumichi" sort="Kuroda, Kazumichi" uniqKey="Kuroda K" first="Kazumichi" last="Kuroda">Kazumichi Kuroda</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Mizuguchi, Masashi" sort="Mizuguchi, Masashi" uniqKey="Mizuguchi M" first="Masashi" last="Mizuguchi">Masashi Mizuguchi</name><affiliation><nlm:aff id="A2"> Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Ushijima, Hiroshi" sort="Ushijima, Hiroshi" uniqKey="Ushijima H" first="Hiroshi" last="Ushijima">Hiroshi Ushijima</name><affiliation><nlm:aff id="A3"> Aino Health Science Center, Aino University, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Mor, Gil" sort="Mor, Gil" uniqKey="Mor G" first="Gil" last="Mor">Gil Mor</name><affiliation><nlm:aff id="A4"> Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine, New Haven, CT, USA</nlm:aff></affiliation></author><author><name sortKey="Hayakawa, Satoshi" sort="Hayakawa, Satoshi" uniqKey="Hayakawa S" first="Satoshi" last="Hayakawa">Satoshi Hayakawa</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19694639</idno><idno type="pmc">3025808</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025808</idno><idno type="RBID">PMC:3025808</idno><idno type="doi">10.1111/j.1600-0897.2009.00723.x</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000564</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000564</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis</title><author><name sortKey="Trinh, Quang Duy" sort="Trinh, Quang Duy" uniqKey="Trinh Q" first="Quang Duy" last="Trinh">Quang Duy Trinh</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation><affiliation><nlm:aff id="A2"> Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Izumi, Yasuyuki" sort="Izumi, Yasuyuki" uniqKey="Izumi Y" first="Yasuyuki" last="Izumi">Yasuyuki Izumi</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Komine Aizawa, Shihoko" sort="Komine Aizawa, Shihoko" uniqKey="Komine Aizawa S" first="Shihoko" last="Komine-Aizawa">Shihoko Komine-Aizawa</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Shibata, Toshikatsu" sort="Shibata, Toshikatsu" uniqKey="Shibata T" first="Toshikatsu" last="Shibata">Toshikatsu Shibata</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Kuroda, Kazumichi" sort="Kuroda, Kazumichi" uniqKey="Kuroda K" first="Kazumichi" last="Kuroda">Kazumichi Kuroda</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Mizuguchi, Masashi" sort="Mizuguchi, Masashi" uniqKey="Mizuguchi M" first="Masashi" last="Mizuguchi">Masashi Mizuguchi</name><affiliation><nlm:aff id="A2"> Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Ushijima, Hiroshi" sort="Ushijima, Hiroshi" uniqKey="Ushijima H" first="Hiroshi" last="Ushijima">Hiroshi Ushijima</name><affiliation><nlm:aff id="A3"> Aino Health Science Center, Aino University, Tokyo, Japan</nlm:aff></affiliation></author><author><name sortKey="Mor, Gil" sort="Mor, Gil" uniqKey="Mor G" first="Gil" last="Mor">Gil Mor</name><affiliation><nlm:aff id="A4"> Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine, New Haven, CT, USA</nlm:aff></affiliation></author><author><name sortKey="Hayakawa, Satoshi" sort="Hayakawa, Satoshi" uniqKey="Hayakawa S" first="Satoshi" last="Hayakawa">Satoshi Hayakawa</name><affiliation><nlm:aff id="A1"> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</nlm:aff></affiliation></author></analytic><series><title level="j">American journal of reproductive immunology (New York, N.Y. : 1989)</title><idno type="ISSN">1046-7408</idno><idno type="eISSN">1600-0897</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Problem</title><p id="P1">Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown.</p></sec><sec id="S2"><title>Method of study</title><p id="P2">The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)-G were also examined.</p></sec><sec id="S3"><title>Results</title><p id="P3">Intracellular localization of viral proteins was observed. Twenty-four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA-G was unchanged.</p></sec><sec id="S4"><title>Conclusion</title><p id="P4">We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA-G expression and subsequent rejection by maternal immune system.</p></sec></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8912860</journal-id><journal-id journal-id-type="pubmed-jr-id">1403</journal-id><journal-id journal-id-type="nlm-ta">Am J Reprod Immunol</journal-id><journal-title>American journal of reproductive immunology (New York, N.Y. : 1989)</journal-title><issn pub-type="ppub">1046-7408</issn><issn pub-type="epub">1600-0897</issn></journal-meta><article-meta><article-id pub-id-type="pmid">19694639</article-id><article-id pub-id-type="pmc">3025808</article-id><article-id pub-id-type="doi">10.1111/j.1600-0897.2009.00723.x</article-id><article-id pub-id-type="manuscript">NIHMS263792</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Trinh</surname><given-names>Quang Duy</given-names></name><xref rid="A1" ref-type="aff">1</xref><xref rid="A2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Izumi</surname><given-names>Yasuyuki</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Komine-Aizawa</surname><given-names>Shihoko</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Shibata</surname><given-names>Toshikatsu</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Shimotai</surname><given-names>Yoshitaka</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Kuroda</surname><given-names>Kazumichi</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Mizuguchi</surname><given-names>Masashi</given-names></name><xref rid="A2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Ushijima</surname><given-names>Hiroshi</given-names></name><xref rid="A3" ref-type="aff">3</xref></contrib><contrib contrib-type="author"><name><surname>Mor</surname><given-names>Gil</given-names></name><xref rid="A4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Hayakawa</surname><given-names>Satoshi</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib></contrib-group><aff id="A1"><label>1</label> Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan</aff><aff id="A2"><label>2</label> Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan</aff><aff id="A3"><label>3</label> Aino Health Science Center, Aino University, Tokyo, Japan</aff><aff id="A4"><label>4</label> Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine, New Haven, CT, USA</aff><author-notes><corresp id="FN1">Correspondence: Satoshi Hayakawa, Nihon University School of Medicine, 30-1 Ohyaguchi Kamimachi, Itabashiku, Tokyo 173-8610, Japan. <email>satoshih@med.nihon-u.ac.jp</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>12</day><month>1</month><year>2011</year></pub-date><pub-date pub-type="ppub"><month>9</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>24</day><month>1</month><year>2011</year></pub-date><volume>62</volume><issue>3</issue><fpage>139</fpage><lpage>146</lpage><abstract><sec id="S1"><title>Problem</title><p id="P1">Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown.</p></sec><sec id="S2"><title>Method of study</title><p id="P2">The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)-G were also examined.</p></sec><sec id="S3"><title>Results</title><p id="P3">Intracellular localization of viral proteins was observed. Twenty-four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA-G was unchanged.</p></sec><sec id="S4"><title>Conclusion</title><p id="P4">We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA-G expression and subsequent rejection by maternal immune system.</p></sec></abstract><kwd-group><kwd>Apoptosis</kwd><kwd>first trimester trophoblast</kwd><kwd>influenza A virus</kwd></kwd-group><contract-num rid="HD1">P01 HD054713-03
||HD</contract-num><contract-sponsor id="HD1">National Institute of Child Health & Human Development : NICHD</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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