CD8+ T Cell Immunity to 2009 Pandemic and Seasonal H1N1 Influenza Viruses
Identifieur interne : 000561 ( Pmc/Corpus ); précédent : 000560; suivant : 000562CD8+ T Cell Immunity to 2009 Pandemic and Seasonal H1N1 Influenza Viruses
Auteurs : Kristin Scheible ; Gang Zhang ; Jane Baer ; Mitra Azadniv ; Kris Lambert ; Gloria Pryhuber ; John J. Treanor ; David J. TophamSource :
- Vaccine [ 0264-410X ] ; 2011.
Abstract
A novel strain of H1N1 influenza A virus (pH1N1) emerged in 2009, causing a worldwide pandemic. Several studies suggest that this virus is antigenically more closely related to human influenza viruses that circulated prior to 1957 than viruses of more recent seasonal influenza varieties. The extent to which individuals who are naïve to the 2009 pH1N1 virus carry cross-reactive CD8+ T cells is not known, but a certain degree of reactivity would be expected since there is substantial conservation among the internal proteins of the virus. In the present study, we examined production of multiple cytokines in response to virus from CD8+ T cells in healthy adult subjects, between 18 and 50 years of age (born post 1957), who had no evidence of exposure to the 2009 pH1N1 virus, and had blood collected prior to the emergence of the pandemic in April of 2009. Human peripheral blood mononuclear cells (PBMC) were stimulated
Url:
DOI: 10.1016/j.vaccine.2010.12.073
PubMed: 21211588
PubMed Central: 3061835
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PMC:3061835Le document en format XML
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<author><name sortKey="Scheible, Kristin" sort="Scheible, Kristin" uniqKey="Scheible K" first="Kristin" last="Scheible">Kristin Scheible</name>
<affiliation><nlm:aff id="A1"> New York Influenza Center of Excellence, David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology; University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
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<affiliation><nlm:aff id="A2"> Division of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Zhang, Gang" sort="Zhang, Gang" uniqKey="Zhang G" first="Gang" last="Zhang">Gang Zhang</name>
<affiliation><nlm:aff id="A3"> Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Baer, Jane" sort="Baer, Jane" uniqKey="Baer J" first="Jane" last="Baer">Jane Baer</name>
<affiliation><nlm:aff id="A1"> New York Influenza Center of Excellence, David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology; University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
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<author><name sortKey="Azadniv, Mitra" sort="Azadniv, Mitra" uniqKey="Azadniv M" first="Mitra" last="Azadniv">Mitra Azadniv</name>
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<author><name sortKey="Lambert, Kris" sort="Lambert, Kris" uniqKey="Lambert K" first="Kris" last="Lambert">Kris Lambert</name>
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<author><name sortKey="Pryhuber, Gloria" sort="Pryhuber, Gloria" uniqKey="Pryhuber G" first="Gloria" last="Pryhuber">Gloria Pryhuber</name>
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<affiliation><nlm:aff id="A4"> Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
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<affiliation><nlm:aff id="A5"> Division of Infectious Diseases, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
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<author><name sortKey="Topham, David J" sort="Topham, David J" uniqKey="Topham D" first="David J." last="Topham">David J. Topham</name>
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<author><name sortKey="Baer, Jane" sort="Baer, Jane" uniqKey="Baer J" first="Jane" last="Baer">Jane Baer</name>
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<author><name sortKey="Azadniv, Mitra" sort="Azadniv, Mitra" uniqKey="Azadniv M" first="Mitra" last="Azadniv">Mitra Azadniv</name>
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</affiliation>
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<affiliation><nlm:aff id="A4"> Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Treanor, John J" sort="Treanor, John J" uniqKey="Treanor J" first="John J." last="Treanor">John J. Treanor</name>
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</affiliation>
<affiliation><nlm:aff id="A5"> Division of Infectious Diseases, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p id="P2">A novel strain of H1N1 influenza A virus (pH1N1) emerged in 2009, causing a worldwide pandemic. Several studies suggest that this virus is antigenically more closely related to human influenza viruses that circulated prior to 1957 than viruses of more recent seasonal influenza varieties. The extent to which individuals who are naïve to the 2009 pH1N1 virus carry cross-reactive CD8+ T cells is not known, but a certain degree of reactivity would be expected since there is substantial conservation among the internal proteins of the virus. In the present study, we examined production of multiple cytokines in response to virus from CD8+ T cells in healthy adult subjects, between 18 and 50 years of age (born post 1957), who had no evidence of exposure to the 2009 pH1N1 virus, and had blood collected prior to the emergence of the pandemic in April of 2009. Human peripheral blood mononuclear cells (PBMC) were stimulated <italic>in vitro</italic>
with a panel of live viruses, and assayed by intracellular cytokine staining and flow cytometry. Although results were variable, most subjects exhibited cytokine positive CD8+ T cells in response to pH1N1. Cytokine producing cells were predominantly single positive (IL2, IFNγ, or TNFα); triple-cytokine producing cells were relatively rare. This result suggests that although many adults carry cross-reactive T cells against the emergent pandemic virus, these cells are in a functionally limited state, possibly because these subjects have not had recent exposure to either seasonal or pandemic influenza strains.</p>
</div>
</front>
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<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8406899</journal-id>
<journal-id journal-id-type="pubmed-jr-id">7945</journal-id>
<journal-id journal-id-type="nlm-ta">Vaccine</journal-id>
<journal-title>Vaccine</journal-title>
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<issn pub-type="epub">1873-2518</issn>
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<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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<title-group><article-title>CD8+ T Cell Immunity to 2009 Pandemic and Seasonal H1N1 Influenza Viruses</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Scheible</surname>
<given-names>Kristin</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
<xref rid="A2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Gang</given-names>
</name>
<xref rid="A3" ref-type="aff">c</xref>
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<contrib contrib-type="author"><name><surname>Baer</surname>
<given-names>Jane</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Azadniv</surname>
<given-names>Mitra</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lambert</surname>
<given-names>Kris</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pryhuber</surname>
<given-names>Gloria</given-names>
</name>
<xref rid="A2" ref-type="aff">b</xref>
<xref rid="A4" ref-type="aff">d</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Treanor</surname>
<given-names>John J.</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
<xref rid="A5" ref-type="aff">e</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Topham</surname>
<given-names>David J.</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
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<aff id="A1"><label>a</label>
New York Influenza Center of Excellence, David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology; University of Rochester Medical Center, Rochester, NY 14642, USA</aff>
<aff id="A2"><label>b</label>
Division of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA</aff>
<aff id="A3"><label>c</label>
Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY 14642, USA</aff>
<aff id="A4"><label>d</label>
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</aff>
<aff id="A5"><label>e</label>
Division of Infectious Diseases, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</aff>
<author-notes><corresp id="FN1"><label>*</label>
To whom correspondence should be addressed: David J. Topham, Ph.D., David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Box 609, Rochester, NY, 14642, Tel: 585-273-1400, Fax: 585-273-2452, <email>David_Topham@urmc.rochester.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>16</day>
<month>2</month>
<year>2011</year>
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<pub-date pub-type="pmc-release"><day>3</day>
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<year>2012</year>
</pub-date>
<volume>29</volume>
<issue>11</issue>
<fpage>2159</fpage>
<lpage>2168</lpage>
<abstract><p id="P2">A novel strain of H1N1 influenza A virus (pH1N1) emerged in 2009, causing a worldwide pandemic. Several studies suggest that this virus is antigenically more closely related to human influenza viruses that circulated prior to 1957 than viruses of more recent seasonal influenza varieties. The extent to which individuals who are naïve to the 2009 pH1N1 virus carry cross-reactive CD8+ T cells is not known, but a certain degree of reactivity would be expected since there is substantial conservation among the internal proteins of the virus. In the present study, we examined production of multiple cytokines in response to virus from CD8+ T cells in healthy adult subjects, between 18 and 50 years of age (born post 1957), who had no evidence of exposure to the 2009 pH1N1 virus, and had blood collected prior to the emergence of the pandemic in April of 2009. Human peripheral blood mononuclear cells (PBMC) were stimulated <italic>in vitro</italic>
with a panel of live viruses, and assayed by intracellular cytokine staining and flow cytometry. Although results were variable, most subjects exhibited cytokine positive CD8+ T cells in response to pH1N1. Cytokine producing cells were predominantly single positive (IL2, IFNγ, or TNFα); triple-cytokine producing cells were relatively rare. This result suggests that although many adults carry cross-reactive T cells against the emergent pandemic virus, these cells are in a functionally limited state, possibly because these subjects have not had recent exposure to either seasonal or pandemic influenza strains.</p>
</abstract>
<kwd-group><kwd>Influenza</kwd>
<kwd>heterosubtypic</kwd>
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<contract-num rid="AI1">N01 AI050020
||AI</contract-num>
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