Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses
Identifieur interne : 000059 ( Pmc/Corpus ); précédent : 000058; suivant : 000060Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses
Auteurs : Hua Yang ; Paul J. Carney ; Vasiliy P. Mishin ; Zhu Guo ; Jessie C. Chang ; David E. Wentworth ; Larisa V. Gubareva ; James StevensSource :
- Journal of Virology [ 0022-538X ] ; 2016.
Abstract
During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.
Url:
DOI: 10.1128/JVI.00180-16
PubMed: 27053557
PubMed Central: 4886766
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<author><name sortKey="Yang, Hua" sort="Yang, Hua" uniqKey="Yang H" first="Hua" last="Yang">Hua Yang</name>
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<author><name sortKey="Carney, Paul J" sort="Carney, Paul J" uniqKey="Carney P" first="Paul J." last="Carney">Paul J. Carney</name>
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<author><name sortKey="Mishin, Vasiliy P" sort="Mishin, Vasiliy P" uniqKey="Mishin V" first="Vasiliy P." last="Mishin">Vasiliy P. Mishin</name>
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<author><name sortKey="Guo, Zhu" sort="Guo, Zhu" uniqKey="Guo Z" first="Zhu" last="Guo">Zhu Guo</name>
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<author><name sortKey="Chang, Jessie C" sort="Chang, Jessie C" uniqKey="Chang J" first="Jessie C." last="Chang">Jessie C. Chang</name>
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<author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name>
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<author><name sortKey="Gubareva, Larisa V" sort="Gubareva, Larisa V" uniqKey="Gubareva L" first="Larisa V." last="Gubareva">Larisa V. Gubareva</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses</title>
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<series><title level="j">Journal of Virology</title>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.</p>
<p><bold>IMPORTANCE</bold>
The H5Nx viruses emerging in North America, Europe, and Asia pose a great public health concern. Here we report a molecular and structural study of the major surface proteins of several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preferences and susceptibilities to antivirals, which are central to pandemic risk assessment.</p>
</div>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Virol</journal-id>
<journal-id journal-id-type="hwp">jvi</journal-id>
<journal-id journal-id-type="pmc">jvi</journal-id>
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<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
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<article-categories><subj-group subj-group-type="heading"><subject>Structure and Assembly</subject>
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<title-group><article-title>Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses</article-title>
<alt-title alt-title-type="running-head">H5Nx Surface Protein Characterization</alt-title>
<alt-title alt-title-type="short-authors">Yang et al.</alt-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Hua</given-names>
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<contrib contrib-type="author"><name><surname>Carney</surname>
<given-names>Paul J.</given-names>
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<contrib contrib-type="author"><name><surname>Mishin</surname>
<given-names>Vasiliy P.</given-names>
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<contrib contrib-type="author"><name><surname>Guo</surname>
<given-names>Zhu</given-names>
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<contrib contrib-type="author"><name><surname>Chang</surname>
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<contrib contrib-type="author"><name><surname>Wentworth</surname>
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<contrib contrib-type="author"><name><surname>Gubareva</surname>
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<contrib contrib-type="author" corresp="yes"><name><surname>Stevens</surname>
<given-names>James</given-names>
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<aff>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA</aff>
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<contrib-group><contrib contrib-type="editor"><name><surname>Schultz-Cherry</surname>
<given-names>S.</given-names>
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<aff>St. Jude Children's Research Hospital</aff>
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<author-notes><corresp id="cor1">Address correspondence to James Stevens, <email>fwb4@cdc.gov</email>
.</corresp>
<fn fn-type="other"><p><bold>Citation</bold>
Yang H, Carney PJ, Mishin VP, Guo Z, Chang JC, Wentworth DE, Gubareva LV, Stevens J. 2016. Molecular characterizations of surface proteins hemagglutinin and neuraminidase from recent H5Nx avian influenza viruses. J Virol 90:5770–5784. doi:<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1128/JVI.00180-16">10.1128/JVI.00180-16</ext-link>
.</p>
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<pub-date pub-type="epreprint"><day>6</day>
<month>4</month>
<year>2016</year>
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<pub-date pub-type="epub"><day>27</day>
<month>5</month>
<year>2016</year>
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<pub-date pub-type="collection"><day>15</day>
<month>6</month>
<year>2016</year>
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<volume>90</volume>
<issue>12</issue>
<fpage>5770</fpage>
<lpage>5784</lpage>
<history><date date-type="received"><day>27</day>
<month>1</month>
<year>2016</year>
</date>
<date date-type="accepted"><day>3</day>
<month>4</month>
<year>2016</year>
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<permissions><copyright-statement>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2016</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
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<self-uri content-type="pdf" xlink:href="zjv01216005770.pdf"></self-uri>
<abstract><title>ABSTRACT</title>
<p>During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.</p>
<p><bold>IMPORTANCE</bold>
The H5Nx viruses emerging in North America, Europe, and Asia pose a great public health concern. Here we report a molecular and structural study of the major surface proteins of several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preferences and susceptibilities to antivirals, which are central to pandemic risk assessment.</p>
</abstract>
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