N-Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor
Identifieur interne : 000861 ( Pmc/Checkpoint ); précédent : 000860; suivant : 000862N-Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor
Auteurs : Nongluk Sriwilaijaroen [Thaïlande, Japon] ; Sachiko Kondo [Japon] ; Hirokazu Yagi [Japon] ; Nobuhiro Takemae [Thaïlande, Japon] ; Takehiko Saito [Thaïlande, Japon] ; Hiroaki Hiramatsu [Japon] ; Koichi Kato [Japon] ; Yasuo Suzuki [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2011.
Abstract
It is known that pigs acted as “mixing vessels” for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to ‘jump species’ resulting in a human outbreak remain unclear.
Url:
DOI: 10.1371/journal.pone.0016302
PubMed: 21347401
PubMed Central: 3036579
Affiliations:
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sortKey="Yagi, Hirokazu" sort="Yagi, Hirokazu" uniqKey="Yagi H" first="Hirokazu" last="Yagi">Hirokazu Yagi</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Takemae, Nobuhiro" sort="Takemae, Nobuhiro" uniqKey="Takemae N" first="Nobuhiro" last="Takemae">Nobuhiro Takemae</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, 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last="Sriwilaijaroen">Nongluk Sriwilaijaroen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani</wicri:regionArea><wicri:noRegion>Pathumthani</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Kondo, Sachiko" sort="Kondo, Sachiko" uniqKey="Kondo S" first="Sachiko" last="Kondo">Sachiko Kondo</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>GLYENCE Co., Ltd., Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>GLYENCE Co., Ltd., Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Yagi, Hirokazu" sort="Yagi, Hirokazu" uniqKey="Yagi H" first="Hirokazu" last="Yagi">Hirokazu Yagi</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Takemae, Nobuhiro" sort="Takemae, Nobuhiro" uniqKey="Takemae N" first="Nobuhiro" last="Takemae">Nobuhiro Takemae</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Saito, Takehiko" sort="Saito, Takehiko" uniqKey="Saito T" first="Takehiko" last="Saito">Takehiko Saito</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Hiramatsu, Hiroaki" sort="Hiramatsu, Hiroaki" uniqKey="Hiramatsu H" first="Hiroaki" last="Hiramatsu">Hiroaki Hiramatsu</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Kato, Koichi" sort="Kato, Koichi" uniqKey="Kato K" first="Koichi" last="Kato">Koichi Kato</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>GLYENCE Co., Ltd., Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>GLYENCE Co., Ltd., Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of National Sciences, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of National Sciences, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff8"><addr-line>The Glycoscience Institute, Ochanomizu University, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>The Glycoscience Institute, Ochanomizu University, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Yasuo" sort="Suzuki, Yasuo" uniqKey="Suzuki Y" first="Yasuo" last="Suzuki">Yasuo Suzuki</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff9"><addr-line>Global COE Program for Innovation in Human Sciences, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Global COE Program for Innovation in Human Sciences, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>It is known that pigs acted as “mixing vessels” for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to ‘jump species’ resulting in a human outbreak remain unclear. <italic>N</italic>-glycans derived from the porcine respiratory tract were enzymatically released, fluorescent labeled with 2-aminopyridine, separated according to charge, size and hydrophobicity, and structurally identified by a two-dimensional (size and hydrophobicity) HPLC mapping technique and MALDI-TOF mass spectrometry before and after exo-glycosidase digestion. We found a 3-, 5-, and 13-fold increases in NeuAcα2-6, a preferable human influenza receptor, over NeuAcα2-3, an avian influenza receptor, from upper and lower parts of the porcine trachea towards the porcine lung, a major target organ for swine virus replication. The large proportion of NeuAcα2-6 may exert selective pressure for selection of influenza variants with altered receptor preference for this human-type α2-6 receptor, a crucial first step for generating a human pandemic.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G Neumann</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Klimov, A" uniqKey="Klimov A">A Klimov</name></author><author><name sortKey="Katz, J" uniqKey="Katz J">J Katz</name></author><author><name sortKey="Regnery, H" uniqKey="Regnery H">H Regnery</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y 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article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21347401</article-id><article-id pub-id-type="pmc">3036579</article-id><article-id pub-id-type="publisher-id">PONE-D-10-02149</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0016302</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Cytochemistry</subject><subj-group><subject>Immunocytochemistry</subject></subj-group></subj-group><subj-group><subject>Glycobiology</subject><subj-group><subject>Glycoproteins</subject></subj-group></subj-group></subj-group><subj-group><subject>Microbiology</subject><subj-group><subject>Virology</subject><subj-group><subject>Viral Transmission and Infection</subject><subj-group><subject>Host Cells</subject><subject>Viral Attachment</subject><subject>Viral Entry</subject></subj-group></subj-group><subj-group><subject>Viral Evolution</subject></subj-group></subj-group></subj-group><subj-group><subject>Molecular Cell Biology</subject><subj-group><subject>Cellular Types</subject><subj-group><subject>Epithelial Cells</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Chemistry</subject><subj-group><subject>Chromatography</subject><subj-group><subject>Ion Exchange Chromatography</subject><subject>Reversed-Phase Chromatography</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Veterinary Science</subject><subj-group><subject>Veterinary Anatomy and Physiology</subject><subj-group><subject>Aerial Respiratory Anatomy</subject></subj-group></subj-group><subj-group><subject>Veterinary Diseases</subject><subj-group><subject>Zoonotic Diseases</subject><subj-group><subject>Animal Influenza</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title><italic>N</italic>-Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor</article-title><alt-title alt-title-type="running-head"><italic>N</italic>-Glycans from Porcine Trachea and Lung</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sriwilaijaroen</surname><given-names>Nongluk</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kondo</surname><given-names>Sachiko</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yagi</surname><given-names>Hirokazu</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Takemae</surname><given-names>Nobuhiro</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Saito</surname><given-names>Takehiko</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hiramatsu</surname><given-names>Hiroaki</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kato</surname><given-names>Koichi</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><xref ref-type="aff" rid="aff7"><sup>7</sup></xref><xref ref-type="aff" rid="aff8"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Suzuki</surname><given-names>Yasuo</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff9"><sup>9</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Graduate School of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan</addr-line></aff><aff id="aff4"><label>4</label><addr-line>GLYENCE Co., Ltd., Aichi, Japan</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Thailand-Japan Zoonotic Diseases Collaboration Center, Chatuchak, Bangkok, Thailand</addr-line></aff><aff id="aff6"><label>6</label><addr-line>Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan</addr-line></aff><aff id="aff7"><label>7</label><addr-line>Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of National Sciences, Aichi, Japan</addr-line></aff><aff id="aff8"><label>8</label><addr-line>The Glycoscience Institute, Ochanomizu University, Tokyo, Japan</addr-line></aff><aff id="aff9"><label>9</label><addr-line>Global COE Program for Innovation in Human Sciences, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Davis</surname><given-names>Todd</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1">Centers for Disease Control and Prevention, United States of America</aff><author-notes><corresp id="cor1">* E-mail: <email>suzukiy@isc.chubu.ac.jp</email></corresp><fn fn-type="con"><p>Conceived and designed the experiments: NS YS NT TS KK. Performed the experiments: NS SK HY NT TS HH. Analyzed the data: NS SK HY HH KK YS. Contributed reagents/materials/analysis tools: TS KK YS. Wrote the paper: NS YS HY KK.</p></fn></author-notes><pub-date pub-type="collection"><year>2011</year></pub-date><pub-date pub-type="epub"><day>9</day><month>2</month><year>2011</year></pub-date><volume>6</volume><issue>2</issue><elocation-id>e16302</elocation-id><history><date date-type="received"><day>1</day><month>9</month><year>2010</year></date><date date-type="accepted"><day>15</day><month>12</month><year>2010</year></date></history><permissions><copyright-statement>Sriwilaijaroen et al.</copyright-statement><copyright-year>2011</copyright-year><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>It is known that pigs acted as “mixing vessels” for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to ‘jump species’ resulting in a human outbreak remain unclear. <italic>N</italic>-glycans derived from the porcine respiratory tract were enzymatically released, fluorescent labeled with 2-aminopyridine, separated according to charge, size and hydrophobicity, and structurally identified by a two-dimensional (size and hydrophobicity) HPLC mapping technique and MALDI-TOF mass spectrometry before and after exo-glycosidase digestion. We found a 3-, 5-, and 13-fold increases in NeuAcα2-6, a preferable human influenza receptor, over NeuAcα2-3, an avian influenza receptor, from upper and lower parts of the porcine trachea towards the porcine lung, a major target organ for swine virus replication. The large proportion of NeuAcα2-6 may exert selective pressure for selection of influenza variants with altered receptor preference for this human-type α2-6 receptor, a crucial first step for generating a human pandemic.</p></abstract><counts><page-count count="8"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>Japon</li><li>Thaïlande</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Thaïlande"><noRegion><name sortKey="Sriwilaijaroen, Nongluk" sort="Sriwilaijaroen, Nongluk" uniqKey="Sriwilaijaroen N" first="Nongluk" last="Sriwilaijaroen">Nongluk Sriwilaijaroen</name></noRegion><name sortKey="Saito, Takehiko" sort="Saito, Takehiko" uniqKey="Saito T" first="Takehiko" last="Saito">Takehiko Saito</name><name sortKey="Takemae, Nobuhiro" sort="Takemae, Nobuhiro" uniqKey="Takemae N" first="Nobuhiro" last="Takemae">Nobuhiro Takemae</name></country><country name="Japon"><noRegion><name sortKey="Sriwilaijaroen, Nongluk" sort="Sriwilaijaroen, Nongluk" uniqKey="Sriwilaijaroen N" first="Nongluk" last="Sriwilaijaroen">Nongluk Sriwilaijaroen</name></noRegion><name sortKey="Hiramatsu, Hiroaki" sort="Hiramatsu, Hiroaki" uniqKey="Hiramatsu H" first="Hiroaki" last="Hiramatsu">Hiroaki Hiramatsu</name><name sortKey="Kato, Koichi" sort="Kato, Koichi" uniqKey="Kato K" first="Koichi" last="Kato">Koichi Kato</name><name sortKey="Kato, Koichi" sort="Kato, Koichi" uniqKey="Kato K" first="Koichi" last="Kato">Koichi Kato</name><name sortKey="Kato, Koichi" sort="Kato, Koichi" uniqKey="Kato K" first="Koichi" last="Kato">Koichi Kato</name><name sortKey="Kato, Koichi" sort="Kato, Koichi" uniqKey="Kato K" first="Koichi" last="Kato">Koichi Kato</name><name sortKey="Kondo, Sachiko" sort="Kondo, Sachiko" uniqKey="Kondo S" first="Sachiko" last="Kondo">Sachiko Kondo</name><name sortKey="Kondo, Sachiko" sort="Kondo, Sachiko" uniqKey="Kondo S" first="Sachiko" last="Kondo">Sachiko Kondo</name><name sortKey="Saito, Takehiko" sort="Saito, Takehiko" uniqKey="Saito T" first="Takehiko" last="Saito">Takehiko Saito</name><name sortKey="Suzuki, Yasuo" sort="Suzuki, Yasuo" uniqKey="Suzuki Y" first="Yasuo" last="Suzuki">Yasuo Suzuki</name><name sortKey="Suzuki, Yasuo" sort="Suzuki, Yasuo" uniqKey="Suzuki Y" first="Yasuo" last="Suzuki">Yasuo Suzuki</name><name sortKey="Takemae, Nobuhiro" sort="Takemae, Nobuhiro" uniqKey="Takemae N" first="Nobuhiro" last="Takemae">Nobuhiro Takemae</name><name sortKey="Yagi, Hirokazu" sort="Yagi, Hirokazu" uniqKey="Yagi H" first="Hirokazu" last="Yagi">Hirokazu Yagi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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