Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus
Identifieur interne : 000809 ( Pmc/Checkpoint ); précédent : 000808; suivant : 000810Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus
Auteurs : Huaxing Wu [République populaire de Chine] ; Beili Li [République populaire de Chine] ; Xue Wang [République populaire de Chine] ; Mingyuan Jin [République populaire de Chine] ; Guonian Wang [République populaire de Chine]Source :
- Molecules [ 1420-3049 ] ; 2011.
Abstract
In the present study, the anti-influenza A (H2N2) virus activity of patchouli alcohol was studied
Url:
DOI: 10.3390/molecules16086489
PubMed: 21814161
PubMed Central: 6264369
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:6264369Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus</title><author><name sortKey="Wu, Huaxing" sort="Wu, Huaxing" uniqKey="Wu H" first="Huaxing" last="Wu">Huaxing Wu</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Li, Beili" sort="Li, Beili" uniqKey="Li B" first="Beili" last="Li">Beili Li</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Mingyuan" sort="Jin, Mingyuan" uniqKey="Jin M" first="Mingyuan" last="Jin">Mingyuan Jin</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Guonian" sort="Wang, Guonian" uniqKey="Wang G" first="Guonian" last="Wang">Guonian Wang</name><affiliation wicri:level="1"><nlm:aff id="af2-molecules-16-06489">Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin 150081, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin 150081</wicri:regionArea><wicri:noRegion>Harbin 150081</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21814161</idno><idno type="pmc">6264369</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264369</idno><idno type="RBID">PMC:6264369</idno><idno type="doi">10.3390/molecules16086489</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000A31</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A31</idno><idno type="wicri:Area/Pmc/Curation">000A31</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A31</idno><idno type="wicri:Area/Pmc/Checkpoint">000809</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000809</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus</title><author><name sortKey="Wu, Huaxing" sort="Wu, Huaxing" uniqKey="Wu H" first="Huaxing" last="Wu">Huaxing Wu</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Li, Beili" sort="Li, Beili" uniqKey="Li B" first="Beili" last="Li">Beili Li</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Mingyuan" sort="Jin, Mingyuan" uniqKey="Jin M" first="Mingyuan" last="Jin">Mingyuan Jin</name><affiliation wicri:level="1"><nlm:aff id="af1-molecules-16-06489">Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Guonian" sort="Wang, Guonian" uniqKey="Wang G" first="Guonian" last="Wang">Guonian Wang</name><affiliation wicri:level="1"><nlm:aff id="af2-molecules-16-06489">Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin 150081, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin 150081</wicri:regionArea><wicri:noRegion>Harbin 150081</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecules</title><idno type="eISSN">1420-3049</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In the present study, the anti-influenza A (H2N2) virus activity of patchouli alcohol was studied <italic>in vitro</italic>, <italic>in vivo</italic> and <italic>in silico</italic>. The CC<sub>50</sub> of patchouli alcohol was above 20 µM. Patchouli alcohol could inhibit influenza virus with an IC<sub>50</sub> of 4.03 ± 0.23 µM. MTT assay showed that the inhibition by patchouli alcohol appears strongly after penetration of the virus into the cell. In the influenza mouse model, patchouli alcohol showed obvious protection against the viral infection at a dose of 5 mg/kg/day. Flexible docking and molecular dynamic simulations indicated that patchouli alcohol was bound to the neuraminidase protein of influenza virus, with an interaction energy of –40.38 kcal mol<sup>–1</sup>. The invariant key active-site residues Asp151, Arg152, Glu119, Glu276 and Tyr406 played important roles during the binding process. Based on spatial and energetic criteria, patchouli alcohol interfered with the NA functions. Results presented here suggest that patchouli alcohol possesses anti-influenza A (H2N2) virus properties, and therefore is a potential source of anti-influenza agents for the pharmaceutical industry.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cox, N J" uniqKey="Cox N">N.J. Cox</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fauci, A S" uniqKey="Fauci A">A.S. Fauci</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Layne, S P" uniqKey="Layne S">S.P. Layne</name></author><author><name sortKey="Monto, A S" uniqKey="Monto A">A.S. Monto</name></author><author><name sortKey="Taubenberger, J K" uniqKey="Taubenberger J">J.K. Taubenberger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubareva, L V" uniqKey="Gubareva L">L.V. Gubareva</name></author><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L. Kaiser</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moscona, A" uniqKey="Moscona A">A. Moscona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E. De Clercq</name></author><author><name sortKey="Neyts, J" uniqKey="Neyts J">J. Neyts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garman, E" uniqKey="Garman E">E. Garman</name></author><author><name sortKey="Laver, G" uniqKey="Laver G">G. Laver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Itzstein, M" uniqKey="Von Itzstein M">M. von Itzstein</name></author><author><name sortKey="Wu, W Y" uniqKey="Wu W">W.Y. Wu</name></author><author><name sortKey="Kok, G B" uniqKey="Kok G">G.B. Kok</name></author><author><name sortKey="Pegg, M S" uniqKey="Pegg M">M.S. Pegg</name></author><author><name sortKey="Dyason, J C" uniqKey="Dyason J">J.C. Dyason</name></author><author><name sortKey="Jin, B" uniqKey="Jin B">B. Jin</name></author><author><name sortKey="Van Phan, T" uniqKey="Van Phan T">T. Van Phan</name></author><author><name sortKey="Smythe, M L" uniqKey="Smythe M">M.L. Smythe</name></author><author><name sortKey="White, H F" uniqKey="White H">H.F. White</name></author><author><name sortKey="Oliver, S W" uniqKey="Oliver S">S.W. Oliver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, C U" uniqKey="Kim C">C.U. Kim</name></author><author><name sortKey="Lew, W" uniqKey="Lew W">W. Lew</name></author><author><name sortKey="Williams, M A" uniqKey="Williams M">M.A. Williams</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author><author><name sortKey="Swaminathan, S" uniqKey="Swaminathan S">S. Swaminathan</name></author><author><name sortKey="Bischofberger, N" uniqKey="Bischofberger N">N. Bischofberger</name></author><author><name sortKey="Chen, M S" uniqKey="Chen M">M.S. Chen</name></author><author><name sortKey="Mendel, D B" uniqKey="Mendel D">D.B. Mendel</name></author><author><name sortKey="Tai, C Y" uniqKey="Tai C">C.Y. Tai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varghese, J N" uniqKey="Varghese J">J.N. Varghese</name></author><author><name sortKey="Smith, P W" uniqKey="Smith P">P.W. Smith</name></author><author><name sortKey="Sollis, S L" uniqKey="Sollis S">S.L. Sollis</name></author><author><name sortKey="Blick, T J" uniqKey="Blick T">T.J. Blick</name></author><author><name sortKey="Sahasrabudhe, A" uniqKey="Sahasrabudhe A">A. Sahasrabudhe</name></author><author><name sortKey="Mckimm Breschkin, J L" uniqKey="Mckimm Breschkin J">J.L. McKimm-Breschkin</name></author><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Atigadda, V R" uniqKey="Atigadda V">V.R. Atigadda</name></author><author><name sortKey="Brouillette, W J" uniqKey="Brouillette W">W.J. Brouillette</name></author><author><name sortKey="Duarte, F" uniqKey="Duarte F">F. Duarte</name></author><author><name sortKey="Babu, Y S" uniqKey="Babu Y">Y.S. Babu</name></author><author><name sortKey="Bantia, S" uniqKey="Bantia S">S. Bantia</name></author><author><name sortKey="Chand, P" uniqKey="Chand P">P. Chand</name></author><author><name sortKey="Chu, N" uniqKey="Chu N">N. Chu</name></author><author><name sortKey="Montgomery, J A" uniqKey="Montgomery J">J.A. Montgomery</name></author><author><name sortKey="Walsh, D A" uniqKey="Walsh D">D.A. Walsh</name></author><author><name sortKey="Sudbeck, E" uniqKey="Sudbeck E">E. Sudbeck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubareva, L V" uniqKey="Gubareva L">L.V. Gubareva</name></author><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L. Kaiser</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brouillette, W J" uniqKey="Brouillette W">W.J. Brouillette</name></author><author><name sortKey="Bajpai, S N" uniqKey="Bajpai S">S.N. Bajpai</name></author><author><name sortKey="Ali, S M" uniqKey="Ali S">S.M. Ali</name></author><author><name sortKey="Velu, S E" uniqKey="Velu S">S.E. Velu</name></author><author><name sortKey="Atigadda, V R" uniqKey="Atigadda V">V.R. Atigadda</name></author><author><name sortKey="Lommer, B S" uniqKey="Lommer B">B.S. Lommer</name></author><author><name sortKey="Finley, J B" uniqKey="Finley J">J.B. Finley</name></author><author><name sortKey="Luo, M" uniqKey="Luo M">M. Luo</name></author><author><name sortKey="Air, G M" uniqKey="Air G">G.M. Air</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Itzstein, M" uniqKey="Von Itzstein M">M. von Itzstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, J" uniqKey="Zhang J">J. Zhang</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Fang, H" uniqKey="Fang H">H. Fang</name></author><author><name sortKey="Xu, W" uniqKey="Xu W">W. Xu</name></author><author><name sortKey="Liu, A" uniqKey="Liu A">A. Liu</name></author><author><name sortKey="Du, G" uniqKey="Du G">G. Du</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubareva, L V" uniqKey="Gubareva L">L.V. Gubareva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kiso, M" uniqKey="Kiso M">M. Kiso</name></author><author><name sortKey="Mitamura, K" uniqKey="Mitamura K">K. Mitamura</name></author><author><name sortKey="Sakai Tagawa, Y" uniqKey="Sakai Tagawa Y">Y. Sakai-Tagawa</name></author><author><name sortKey="Shiraishi, K" uniqKey="Shiraishi K">K. Shiraishi</name></author><author><name sortKey="Kawakami, C" uniqKey="Kawakami C">C. Kawakami</name></author><author><name sortKey="Kimura, K" uniqKey="Kimura K">K. Kimura</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author><author><name sortKey="Sugaya, N" uniqKey="Sugaya N">N. Sugaya</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kiyohara, H" uniqKey="Kiyohara H">H. Kiyohara</name></author><author><name sortKey="Ichino, C" uniqKey="Ichino C">C. Ichino</name></author><author><name sortKey="Kawamura, Y" uniqKey="Kawamura Y">Y. Kawamura</name></author><author><name sortKey="Nagai, T" uniqKey="Nagai T">T. Nagai</name></author><author><name sortKey="Sato, N" uniqKey="Sato N">N. Sato</name></author><author><name sortKey="Yamada, H" uniqKey="Yamada H">H. Yamada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsuchiya, Y" uniqKey="Tsuchiya Y">Y. Tsuchiya</name></author><author><name sortKey="Shimizu, M" uniqKey="Shimizu M">M. Shimizu</name></author><author><name sortKey="Hiyama, Y" uniqKey="Hiyama Y">Y. Hiyama</name></author><author><name sortKey="Itoh, K" uniqKey="Itoh K">K. Itoh</name></author><author><name sortKey="Hashimoto, Y" uniqKey="Hashimoto Y">Y. Hashimoto</name></author><author><name sortKey="Nakayama, M" uniqKey="Nakayama M">M. Nakayama</name></author><author><name sortKey="Horie, T" uniqKey="Horie T">T. Horie</name></author><author><name sortKey="Morita, N" uniqKey="Morita N">N. Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, H" uniqKey="Fan H">H. Fan</name></author><author><name sortKey="Ooi, A" uniqKey="Ooi A">A. Ooi</name></author><author><name sortKey="Tan, Y W" uniqKey="Tan Y">Y.W. Tan</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S. Wang</name></author><author><name sortKey="Fang, S" uniqKey="Fang S">S. Fang</name></author><author><name sortKey="Liu, D X" uniqKey="Liu D">D.X. Liu</name></author><author><name sortKey="Lescar, J" uniqKey="Lescar J">J. Lescar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Y W" uniqKey="Tan Y">Y.W. Tan</name></author><author><name sortKey="Fang, S" uniqKey="Fang S">S. Fang</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H. Fan</name></author><author><name sortKey="Lescar, J" uniqKey="Lescar J">J. Lescar</name></author><author><name sortKey="Liu, D X" uniqKey="Liu D">D.X. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jayaram, H" uniqKey="Jayaram H">H. Jayaram</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H. Fan</name></author><author><name sortKey="Bowman, B R" uniqKey="Bowman B">B.R. Bowman</name></author><author><name sortKey="Ooi, A" uniqKey="Ooi A">A. Ooi</name></author><author><name sortKey="Jayaram, J" uniqKey="Jayaram J">J. Jayaram</name></author><author><name sortKey="Collisson, E W" uniqKey="Collisson E">E.W. Collisson</name></author><author><name sortKey="Lescar, J" uniqKey="Lescar J">J. Lescar</name></author><author><name sortKey="Prasad, B V" uniqKey="Prasad B">B.V. Prasad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masukawa, K M" uniqKey="Masukawa K">K.M. Masukawa</name></author><author><name sortKey="Kollman, P A" uniqKey="Kollman P">P.A. Kollman</name></author><author><name sortKey="Kuntz, I D" uniqKey="Kuntz I">I.D. Kuntz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonnet, P" uniqKey="Bonnet P">P. Bonnet</name></author><author><name sortKey="Bryce, R A" uniqKey="Bryce R">R.A. Bryce</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonnet, P" uniqKey="Bonnet P">P. Bonnet</name></author><author><name sortKey="Bryce, R A" uniqKey="Bryce R">R.A. Bryce</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z W" uniqKey="Yang Z">Z.W. Yang</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G. Yang</name></author><author><name sortKey="Zu, Y G" uniqKey="Zu Y">Y.G. Zu</name></author><author><name sortKey="Fu, Y J" uniqKey="Fu Y">Y.J. Fu</name></author><author><name sortKey="Zhou, L J" uniqKey="Zhou L">L.J. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G. Yang</name></author><author><name sortKey="Zu, Y" uniqKey="Zu Y">Y. Zu</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y. Fu</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Nie, Y" uniqKey="Nie Y">Y. Nie</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G. Yang</name></author><author><name sortKey="Zu, Y" uniqKey="Zu Y">Y. Zu</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y. Fu</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oakley, A J" uniqKey="Oakley A">A.J. Oakley</name></author><author><name sortKey="Barrett, S" uniqKey="Barrett S">S. Barrett</name></author><author><name sortKey="Peat, T S" uniqKey="Peat T">T.S. Peat</name></author><author><name sortKey="Newman, J" uniqKey="Newman J">J. Newman</name></author><author><name sortKey="Streltsov, V A" uniqKey="Streltsov V">V.A. Streltsov</name></author><author><name sortKey="Waddington, L" uniqKey="Waddington L">L. Waddington</name></author><author><name sortKey="Saito, T" uniqKey="Saito T">T. Saito</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M. Tashiro</name></author><author><name sortKey="Mckimm Breschkin, J L" uniqKey="Mckimm Breschkin J">J.L. McKimm-Breschkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stoll, V" uniqKey="Stoll V">V. Stoll</name></author><author><name sortKey="Stewart, K D" uniqKey="Stewart K">K.D. Stewart</name></author><author><name sortKey="Maring, C J" uniqKey="Maring C">C.J. Maring</name></author><author><name sortKey="Muchmore, S" uniqKey="Muchmore S">S. Muchmore</name></author><author><name sortKey="Giranda, V" uniqKey="Giranda V">V. Giranda</name></author><author><name sortKey="Gu, Y G Y" uniqKey="Gu Y">Y.-G.Y. Gu</name></author><author><name sortKey="Wang, G" uniqKey="Wang G">G. Wang</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Sun, M" uniqKey="Sun M">M. Sun</name></author><author><name sortKey="Zhao, C" uniqKey="Zhao C">C. Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aruksakunwong, O" uniqKey="Aruksakunwong O">O. Aruksakunwong</name></author><author><name sortKey="Malaisree, M" uniqKey="Malaisree M">M. Malaisree</name></author><author><name sortKey="Decha, P" uniqKey="Decha P">P. Decha</name></author><author><name sortKey="Sompornpisut, P" uniqKey="Sompornpisut P">P. Sompornpisut</name></author><author><name sortKey="Parasuk, V" uniqKey="Parasuk V">V. Parasuk</name></author><author><name sortKey="Pianwanit, S" uniqKey="Pianwanit S">S. Pianwanit</name></author><author><name sortKey="Hannongbua, S" uniqKey="Hannongbua S">S. Hannongbua</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author><author><name sortKey="Varghese, J N" uniqKey="Varghese J">J.N. Varghese</name></author><author><name sortKey="Laver, W G" uniqKey="Laver W">W.G. Laver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jedrzejas, M J" uniqKey="Jedrzejas M">M.J. Jedrzejas</name></author><author><name sortKey="Singh, S" uniqKey="Singh S">S. Singh</name></author><author><name sortKey="Brouillette, W J" uniqKey="Brouillette W">W.J. Brouillette</name></author><author><name sortKey="Laver, W G" uniqKey="Laver W">W.G. Laver</name></author><author><name sortKey="Air, G M" uniqKey="Air G">G.M. Air</name></author><author><name sortKey="Luo, M" uniqKey="Luo M">M. Luo</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Head, J D" uniqKey="Head J">J.D. Head</name></author><author><name sortKey="Zerner, M C" uniqKey="Zerner M">M.C. Zerner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z W" uniqKey="Yang Z">Z.W. Yang</name></author><author><name sortKey="Zu, Y G" uniqKey="Zu Y">Y.G. Zu</name></author><author><name sortKey="Wu, X M" uniqKey="Wu X">X.M. Wu</name></author><author><name sortKey="Liu, C B" uniqKey="Liu C">C.B. Liu</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z W" uniqKey="Yang Z">Z.W. Yang</name></author><author><name sortKey="Wu, N" uniqKey="Wu N">N. Wu</name></author><author><name sortKey="Fu, F J" uniqKey="Fu F">F.J. Fu</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G. Yang</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Zu, Y G" uniqKey="Zu Y">Y.G. Zu</name></author><author><name sortKey="Efferth, T" uniqKey="Efferth T">T. Efferth</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Adelman, S A" uniqKey="Adelman S">S.A. Adelman</name></author><author><name sortKey="Doll, J D" uniqKey="Doll J">J.D. Doll</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Molecules</journal-id><journal-id journal-id-type="iso-abbrev">Molecules</journal-id><journal-id journal-id-type="publisher-id">molecules</journal-id><journal-title-group><journal-title>Molecules</journal-title></journal-title-group><issn pub-type="epub">1420-3049</issn><publisher><publisher-name>MDPI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21814161</article-id><article-id pub-id-type="pmc">6264369</article-id><article-id pub-id-type="doi">10.3390/molecules16086489</article-id><article-id pub-id-type="publisher-id">molecules-16-06489</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Huaxing</given-names></name><xref ref-type="aff" rid="af1-molecules-16-06489">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Beili</given-names></name><xref ref-type="aff" rid="af1-molecules-16-06489">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xue</given-names></name><xref ref-type="aff" rid="af1-molecules-16-06489">1</xref></contrib><contrib contrib-type="author"><name><surname>Jin</surname><given-names>Mingyuan</given-names></name><xref ref-type="aff" rid="af1-molecules-16-06489">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Guonian</given-names></name><xref ref-type="aff" rid="af2-molecules-16-06489">2</xref><xref rid="c1-molecules-16-06489" ref-type="corresp">*</xref></contrib></contrib-group><aff id="af1-molecules-16-06489"><label>1</label>Department of Endoscopy, the Third Affiliated Hospital, Harbin Medical University, Harbin 150040, China</aff><aff id="af2-molecules-16-06489"><label>2</label>Department of Anesthesiology, the Third Affiliated Hospital, Harbin Medical University, Harbin 150081, China</aff><author-notes><corresp id="c1-molecules-16-06489"><label>*</label> Author to whom correspondence should be addressed; Email: <email>gracewhx1964@yahoo.cn</email>; Tel.: +86-451-86298237; Fax: +86-451-86298811.</corresp></author-notes><pub-date pub-type="epub"><day>03</day><month>8</month><year>2011</year></pub-date><pub-date pub-type="collection"><month>8</month><year>2011</year></pub-date><volume>16</volume><issue>8</issue><fpage>6489</fpage><lpage>6501</lpage><history><date date-type="received"><day>13</day><month>5</month><year>2011</year></date><date date-type="rev-recd"><day>25</day><month>7</month><year>2011</year></date><date date-type="accepted"><day>28</day><month>7</month><year>2011</year></date></history><permissions><copyright-statement>© 2011 by the authors;</copyright-statement><copyright-year>2011</copyright-year><license license-type="open-access"><license-p>licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>).</license-p></license></permissions><abstract><p>In the present study, the anti-influenza A (H2N2) virus activity of patchouli alcohol was studied <italic>in vitro</italic>, <italic>in vivo</italic> and <italic>in silico</italic>. The CC<sub>50</sub> of patchouli alcohol was above 20 µM. Patchouli alcohol could inhibit influenza virus with an IC<sub>50</sub> of 4.03 ± 0.23 µM. MTT assay showed that the inhibition by patchouli alcohol appears strongly after penetration of the virus into the cell. In the influenza mouse model, patchouli alcohol showed obvious protection against the viral infection at a dose of 5 mg/kg/day. Flexible docking and molecular dynamic simulations indicated that patchouli alcohol was bound to the neuraminidase protein of influenza virus, with an interaction energy of –40.38 kcal mol<sup>–1</sup>. The invariant key active-site residues Asp151, Arg152, Glu119, Glu276 and Tyr406 played important roles during the binding process. Based on spatial and energetic criteria, patchouli alcohol interfered with the NA functions. Results presented here suggest that patchouli alcohol possesses anti-influenza A (H2N2) virus properties, and therefore is a potential source of anti-influenza agents for the pharmaceutical industry.</p></abstract><kwd-group><kwd>patchouli alcohol</kwd><kwd>docking</kwd><kwd>neuraminidase</kwd><kwd>MTT</kwd><kwd>influenza virus</kwd><kwd>mice</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Huaxing" sort="Wu, Huaxing" uniqKey="Wu H" first="Huaxing" last="Wu">Huaxing Wu</name></noRegion><name sortKey="Jin, Mingyuan" sort="Jin, Mingyuan" uniqKey="Jin M" first="Mingyuan" last="Jin">Mingyuan Jin</name><name sortKey="Li, Beili" sort="Li, Beili" uniqKey="Li B" first="Beili" last="Li">Beili Li</name><name sortKey="Wang, Guonian" sort="Wang, Guonian" uniqKey="Wang G" first="Guonian" last="Wang">Guonian Wang</name><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000809 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000809 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:6264369
|texte= Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:21814161" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |