Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics
Identifieur interne : 000740 ( Pmc/Checkpoint ); précédent : 000739; suivant : 000741Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics
Auteurs : Gunisha Pasricha ; Akhilesh C. Mishra ; Alok K. ChakrabartiSource :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2012.
Abstract
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DOI: 10.1111/j.1750-2659.2012.00400.x
PubMed: 22788742
PubMed Central: 5780999
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics</title><author><name sortKey="Pasricha, Gunisha" sort="Pasricha, Gunisha" uniqKey="Pasricha G" first="Gunisha" last="Pasricha">Gunisha Pasricha</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author><author><name sortKey="Mishra, Akhilesh C" sort="Mishra, Akhilesh C" uniqKey="Mishra A" first="Akhilesh C." last="Mishra">Akhilesh C. Mishra</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author><author><name sortKey="Chakrabarti, Alok K" sort="Chakrabarti, Alok K" uniqKey="Chakrabarti A" first="Alok K." last="Chakrabarti">Alok K. Chakrabarti</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22788742</idno><idno type="pmc">5780999</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780999</idno><idno type="RBID">PMC:5780999</idno><idno type="doi">10.1111/j.1750-2659.2012.00400.x</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000979</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000979</idno><idno type="wicri:Area/Pmc/Curation">000979</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000979</idno><idno type="wicri:Area/Pmc/Checkpoint">000740</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000740</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics</title><author><name sortKey="Pasricha, Gunisha" sort="Pasricha, Gunisha" uniqKey="Pasricha G" first="Gunisha" last="Pasricha">Gunisha Pasricha</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author><author><name sortKey="Mishra, Akhilesh C" sort="Mishra, Akhilesh C" uniqKey="Mishra A" first="Akhilesh C." last="Mishra">Akhilesh C. Mishra</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author><author><name sortKey="Chakrabarti, Alok K" sort="Chakrabarti, Alok K" uniqKey="Chakrabarti A" first="Alok K." last="Chakrabarti">Alok K. Chakrabarti</name><affiliation><nlm:aff id="aff-1-1"></nlm:aff></affiliation></author></analytic><series><title level="j">Influenza and Other Respiratory Viruses</title><idno type="ISSN">1750-2640</idno><idno type="eISSN">1750-2659</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Please cite this paper as:</italic> Pasricha <italic>et al.</italic> (2012) Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the Influenza A virus subtypes responsible for the 20th‐century pandemics. Influenza and Other Respiratory Viruses 7(4), 497–505.</p><p><bold>Background </bold> PB1F2 is the 11th protein of influenza A virus translated from +1 alternate reading frame of PB1 gene. Since the discovery, varying sizes and functions of the PB1F2 protein of influenza A viruses have been reported. Selection of PB1 gene segment in the pandemics, variable size and pleiotropic effect of PB1F2 intrigued us to analyze amino acid sequences of this protein in various influenza A viruses.</p><p><bold>Methods </bold> Amino acid sequences for PB1F2 protein of influenza A H5N1, H1N1, H2N2, and H3N2 subtypes were obtained from Influenza Research Database. Multiple sequence alignments of the PB1F2 protein sequences of the aforementioned subtypes were used to determine the size, variable and conserved domains and to perform mutational analysis.</p><p><bold>Results </bold> Analysis showed that 96·4% of the H5N1 influenza viruses harbored full‐length PB1F2 protein. Except for the 2009 pandemic H1N1 virus, all the subtypes of the 20th‐century pandemic influenza viruses contained full‐length PB1F2 protein. Through the years, PB1F2 protein of the H1N1 and H3N2 viruses has undergone much variation. PB1F2 protein sequences of H5N1 viruses showed both human‐ and avian host‐specific conserved domains. Global database of PB1F2 protein revealed that N66S mutation was present only in 3·8% of the H5N1 strains. We found a novel mutation, N84S in the PB1F2 protein of 9·35% of the highly pathogenic avian influenza H5N1 influenza viruses.</p><p><bold>Conclusions </bold> Varying sizes and mutations of the PB1F2 protein in different influenza A virus subtypes with pandemic potential were obtained. There was genetic divergence of the protein in various hosts which highlighted the host‐specific evolution of the virus. However, studies are required to correlate this sequence variability with the virulence and pathogenicity.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Knipe, Dm" uniqKey="Knipe D">DM Knipe</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="iso-abbrev">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="doi">10.1111/(ISSN)1750-2659</journal-id><journal-id journal-id-type="publisher-id">IRV</journal-id><journal-title-group><journal-title>Influenza and Other Respiratory Viruses</journal-title></journal-title-group><issn pub-type="ppub">1750-2640</issn><issn pub-type="epub">1750-2659</issn><publisher><publisher-name>Blackwell Publishing Ltd</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22788742</article-id><article-id pub-id-type="pmc">5780999</article-id><article-id pub-id-type="doi">10.1111/j.1750-2659.2012.00400.x</article-id><article-id pub-id-type="publisher-id">IRV400</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group><subj-group subj-group-type="overline"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics</article-title><alt-title alt-title-type="left-running-head">Pasricha <italic>et al.</italic></alt-title><alt-title alt-title-type="right-running-head">Amino acid sequence analysis of PB1F2 protein</alt-title></title-group><contrib-group><contrib id="cr1" contrib-type="author"><name><surname>Pasricha</surname><given-names>Gunisha</given-names></name><xref ref-type="aff" rid="aff-1-1"><sup>1</sup></xref></contrib><contrib id="cr2" contrib-type="author"><name><surname>Mishra</surname><given-names>Akhilesh C.</given-names></name><xref ref-type="aff" rid="aff-1-1"><sup>1</sup></xref></contrib><contrib id="cr3" contrib-type="author"><name><surname>Chakrabarti</surname><given-names>Alok K.</given-names></name><xref ref-type="aff" rid="aff-1-1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff-1-1"><label><sup>1</sup></label>Microbial Containment Complex, National Institute of Virology, Pashan, Pune, India.</aff><author-notes><corresp id="correspondenceTo">Alok K. Chakrabarti, Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021, India.
E‐mail: <email>aloke8@yahoo.com</email></corresp></author-notes><pub-date pub-type="epub"><day>13</day><month>7</month><year>2012</year></pub-date><pub-date pub-type="ppub"><month>7</month><year>2013</year></pub-date><volume>7</volume><issue>4</issue><issue-id pub-id-type="doi">10.1111/irv.2013.7.issue-4</issue-id><fpage>497</fpage><lpage>505</lpage><history>Accepted 16 May 2012. Published Online 13 July 2012.</history><permissions><copyright-statement content-type="article-copyright">© 2012 John Wiley & Sons Ltd</copyright-statement></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="file:IRV-7-497.pdf"></self-uri><abstract><p><italic>Please cite this paper as:</italic> Pasricha <italic>et al.</italic> (2012) Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the Influenza A virus subtypes responsible for the 20th‐century pandemics. Influenza and Other Respiratory Viruses 7(4), 497–505.</p><p><bold>Background </bold> PB1F2 is the 11th protein of influenza A virus translated from +1 alternate reading frame of PB1 gene. Since the discovery, varying sizes and functions of the PB1F2 protein of influenza A viruses have been reported. Selection of PB1 gene segment in the pandemics, variable size and pleiotropic effect of PB1F2 intrigued us to analyze amino acid sequences of this protein in various influenza A viruses.</p><p><bold>Methods </bold> Amino acid sequences for PB1F2 protein of influenza A H5N1, H1N1, H2N2, and H3N2 subtypes were obtained from Influenza Research Database. Multiple sequence alignments of the PB1F2 protein sequences of the aforementioned subtypes were used to determine the size, variable and conserved domains and to perform mutational analysis.</p><p><bold>Results </bold> Analysis showed that 96·4% of the H5N1 influenza viruses harbored full‐length PB1F2 protein. Except for the 2009 pandemic H1N1 virus, all the subtypes of the 20th‐century pandemic influenza viruses contained full‐length PB1F2 protein. Through the years, PB1F2 protein of the H1N1 and H3N2 viruses has undergone much variation. PB1F2 protein sequences of H5N1 viruses showed both human‐ and avian host‐specific conserved domains. Global database of PB1F2 protein revealed that N66S mutation was present only in 3·8% of the H5N1 strains. We found a novel mutation, N84S in the PB1F2 protein of 9·35% of the highly pathogenic avian influenza H5N1 influenza viruses.</p><p><bold>Conclusions </bold> Varying sizes and mutations of the PB1F2 protein in different influenza A virus subtypes with pandemic potential were obtained. There was genetic divergence of the protein in various hosts which highlighted the host‐specific evolution of the virus. However, studies are required to correlate this sequence variability with the virulence and pathogenicity.</p></abstract><kwd-group><kwd id="k1">Influenza A viruses</kwd><kwd id="k2">mutation</kwd><kwd id="k3">pandemics</kwd><kwd id="k4">PB1F2 protein</kwd><kwd id="k5">truncation</kwd></kwd-group><counts><fig-count count="3"></fig-count><table-count count="4"></table-count><page-count count="9"></page-count></counts><custom-meta-group><custom-meta><meta-name>source-schema-version-number</meta-name><meta-value>2.0</meta-value></custom-meta><custom-meta><meta-name>cover-date</meta-name><meta-value>July 2013</meta-value></custom-meta><custom-meta><meta-name>details-of-publishers-convertor</meta-name><meta-value>Converter:WILEY_ML3GV2_TO_NLMPMC version:4.6.9 mode:remove_FC converted:04.11.2015</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chakrabarti, Alok K" sort="Chakrabarti, Alok K" uniqKey="Chakrabarti A" first="Alok K." last="Chakrabarti">Alok K. Chakrabarti</name><name sortKey="Mishra, Akhilesh C" sort="Mishra, Akhilesh C" uniqKey="Mishra A" first="Akhilesh C." last="Mishra">Akhilesh C. Mishra</name><name sortKey="Pasricha, Gunisha" sort="Pasricha, Gunisha" uniqKey="Pasricha G" first="Gunisha" last="Pasricha">Gunisha Pasricha</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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