Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model
Identifieur interne : 000528 ( Pmc/Checkpoint ); précédent : 000527; suivant : 000529Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model
Auteurs : Irina Isakova-Sivak [États-Unis, Russie] ; Li-Mei Chen [États-Unis] ; Melissa Bourgeois [États-Unis] ; Yumiko Matsuoka [États-Unis] ; J. Theo M. Voeten [Pays-Bas] ; Jacco G. M. Heldens [Pays-Bas] ; Han Van Den Bosch [Pays-Bas] ; Alexander Klimov [États-Unis] ; Larisa Rudenko [Russie] ; Nancy J. Cox [États-Unis] ; Ruben O. Donis [États-Unis]Source :
- Clinical and Vaccine Immunology : CVI [ 1556-6811 ] ; 2014.
Abstract
Live attenuated influenza vaccines (LAIV) offer significant advantages over subunit or split inactivated vaccines to mitigate an eventual influenza pandemic, including simpler manufacturing processes and more cross-protective immune responses. Using an established reverse genetics (rg) system for wild-type (
Url:
DOI: 10.1128/CVI.00819-13
PubMed: 24648485
PubMed Central: 4018889
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4018889Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model</title>
<author><name sortKey="Isakova Sivak, Irina" sort="Isakova Sivak, Irina" uniqKey="Isakova Sivak I" first="Irina" last="Isakova-Sivak">Irina Isakova-Sivak</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea>
<wicri:noRegion>St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bourgeois, Melissa" sort="Bourgeois, Melissa" uniqKey="Bourgeois M" first="Melissa" last="Bourgeois">Melissa Bourgeois</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Voeten, J Theo M" sort="Voeten, J Theo M" uniqKey="Voeten J" first="J. Theo M." last="Voeten">J. Theo M. Voeten</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Heldens, Jacco G M" sort="Heldens, Jacco G M" uniqKey="Heldens J" first="Jacco G. M." last="Heldens">Jacco G. M. Heldens</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Van Den Bosch, Han" sort="Van Den Bosch, Han" uniqKey="Van Den Bosch H" first="Han" last="Van Den Bosch">Han Van Den Bosch</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Klimov, Alexander" sort="Klimov, Alexander" uniqKey="Klimov A" first="Alexander" last="Klimov">Alexander Klimov</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea>
<wicri:noRegion>St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J." last="Cox">Nancy J. Cox</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24648485</idno>
<idno type="pmc">4018889</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018889</idno>
<idno type="RBID">PMC:4018889</idno>
<idno type="doi">10.1128/CVI.00819-13</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000615</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000615</idno>
<idno type="wicri:Area/Pmc/Curation">000615</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000615</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000528</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000528</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model</title>
<author><name sortKey="Isakova Sivak, Irina" sort="Isakova Sivak, Irina" uniqKey="Isakova Sivak I" first="Irina" last="Isakova-Sivak">Irina Isakova-Sivak</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea>
<wicri:noRegion>St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bourgeois, Melissa" sort="Bourgeois, Melissa" uniqKey="Bourgeois M" first="Melissa" last="Bourgeois">Melissa Bourgeois</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Voeten, J Theo M" sort="Voeten, J Theo M" uniqKey="Voeten J" first="J. Theo M." last="Voeten">J. Theo M. Voeten</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Heldens, Jacco G M" sort="Heldens, Jacco G M" uniqKey="Heldens J" first="Jacco G. M." last="Heldens">Jacco G. M. Heldens</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Van Den Bosch, Han" sort="Van Den Bosch, Han" uniqKey="Van Den Bosch H" first="Han" last="Van Den Bosch">Han Van Den Bosch</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Nobilon International BV, Boxmeer, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Nobilon International BV, Boxmeer</wicri:regionArea>
<wicri:noRegion>Boxmeer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Klimov, Alexander" sort="Klimov, Alexander" uniqKey="Klimov A" first="Alexander" last="Klimov">Alexander Klimov</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</nlm:aff>
<country xml:lang="fr">Russie</country>
<wicri:regionArea>Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea>
<wicri:noRegion>St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J." last="Cox">Nancy J. Cox</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Clinical and Vaccine Immunology : CVI</title>
<idno type="ISSN">1556-6811</idno>
<idno type="eISSN">1556-679X</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Live attenuated influenza vaccines (LAIV) offer significant advantages over subunit or split inactivated vaccines to mitigate an eventual influenza pandemic, including simpler manufacturing processes and more cross-protective immune responses. Using an established reverse genetics (rg) system for wild-type (<italic>wt</italic>
) A/Leningrad/134/1957 and cold-adapted (<italic>ca</italic>
) A/Leningrad/134/17/1957 (Len17) master donor virus (MDV), we produced and characterized three rg H5N1 reassortant viruses carrying modified HA and intact NA genes from either A/Vietnam/1203/2004 (H5N1, VN1203, clade 1) or A/Egypt/321/2007 (H5N1, EG321, clade 2) virus. A mouse model of infection was used to determine the infectivity and tissue tropism of the parental <italic>wt</italic>
viruses compared to the <italic>ca</italic>
master donor viruses as well as the H5N1 reassortants. All <italic>ca</italic>
viruses showed reduced replication in lungs and enhanced replication in nasal epithelium. In addition, the H5N1 HA and NA enhanced replication in lungs unless it was restricted by the internal genes of the <italic>ca</italic>
MDV. Mice inoculated twice 4 weeks apart with the H5N1 reassortant LAIV candidate viruses developed serum hemagglutination inhibition HI and IgA antibody titers to the homologous and heterologous viruses consistent with protective immunity. These animals remained healthy after challenge inoculation with a lethal dose with homologous or heterologous <italic>wt</italic>
H5N1 highly pathogenic avian influenza (HPAI) viruses. The profiles of viral replication in respiratory tissues and the immunogenicity and protective efficacy characteristics of the two <italic>ca</italic>
H5N1 candidate LAIV viruses warrant further development into a vaccine for human use.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Vaccine Immunol</journal-id>
<journal-id journal-id-type="iso-abbrev">Clin. Vaccine Immunol</journal-id>
<journal-id journal-id-type="hwp">cdli</journal-id>
<journal-id journal-id-type="pmc">cvi</journal-id>
<journal-id journal-id-type="publisher-id">CVI</journal-id>
<journal-title-group><journal-title>Clinical and Vaccine Immunology : CVI</journal-title>
</journal-title-group>
<issn pub-type="ppub">1556-6811</issn>
<issn pub-type="epub">1556-679X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24648485</article-id>
<article-id pub-id-type="pmc">4018889</article-id>
<article-id pub-id-type="publisher-id">00819-13</article-id>
<article-id pub-id-type="doi">10.1128/CVI.00819-13</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Vaccines</subject>
</subj-group>
</article-categories>
<title-group><article-title>Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Isakova-Sivak</surname>
<given-names>Irina</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Li-Mei</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bourgeois</surname>
<given-names>Melissa</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Matsuoka</surname>
<given-names>Yumiko</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Voeten</surname>
<given-names>J. Theo M.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Heldens</surname>
<given-names>Jacco G. M.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>van den Bosch</surname>
<given-names>Han</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Klimov</surname>
<given-names>Alexander</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="author-notes" rid="fn2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rudenko</surname>
<given-names>Larisa</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cox</surname>
<given-names>Nancy J.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Donis</surname>
<given-names>Ruben O.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
Influenza Division, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA</aff>
<aff id="aff2"><label>b</label>
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</aff>
<aff id="aff3"><label>c</label>
Nobilon International BV, Boxmeer, The Netherlands</aff>
</contrib-group>
<contrib-group><contrib contrib-type="editor"><name><surname>Plotkin</surname>
<given-names>S. A.</given-names>
</name>
<role>Editor</role>
</contrib>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to Ruben O. Donis, <email>rvd6@cdc.gov</email>
.</corresp>
<fn id="fn1" fn-type="present-address"><label>*</label>
<p>Present address: Melissa Bourgeois, Merck Animal Health, Summit, New Jersey, USA; Yumiko Matsuoka, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA; J. Theo M. Voeten, Merck Animal Health, Summit, New Jersey, USA; Jacco G. M. Heldens, Merck Animal Health, Summit, New Jersey, USA; Han van den Bosch, Athena Institute, Free University, Amsterdam, The Netherlands.</p>
</fn>
<fn id="fn2" fn-type="deceased"><label>†</label>
<p>Deceased.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>5</month>
<year>2014</year>
</pub-date>
<volume>21</volume>
<issue>5</issue>
<fpage>722</fpage>
<lpage>731</lpage>
<history><date date-type="received"><day>26</day>
<month>12</month>
<year>2013</year>
</date>
<date date-type="rev-request"><day>21</day>
<month>1</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>11</day>
<month>3</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zcd00514000722.pdf"></self-uri>
<abstract><p>Live attenuated influenza vaccines (LAIV) offer significant advantages over subunit or split inactivated vaccines to mitigate an eventual influenza pandemic, including simpler manufacturing processes and more cross-protective immune responses. Using an established reverse genetics (rg) system for wild-type (<italic>wt</italic>
) A/Leningrad/134/1957 and cold-adapted (<italic>ca</italic>
) A/Leningrad/134/17/1957 (Len17) master donor virus (MDV), we produced and characterized three rg H5N1 reassortant viruses carrying modified HA and intact NA genes from either A/Vietnam/1203/2004 (H5N1, VN1203, clade 1) or A/Egypt/321/2007 (H5N1, EG321, clade 2) virus. A mouse model of infection was used to determine the infectivity and tissue tropism of the parental <italic>wt</italic>
viruses compared to the <italic>ca</italic>
master donor viruses as well as the H5N1 reassortants. All <italic>ca</italic>
viruses showed reduced replication in lungs and enhanced replication in nasal epithelium. In addition, the H5N1 HA and NA enhanced replication in lungs unless it was restricted by the internal genes of the <italic>ca</italic>
MDV. Mice inoculated twice 4 weeks apart with the H5N1 reassortant LAIV candidate viruses developed serum hemagglutination inhibition HI and IgA antibody titers to the homologous and heterologous viruses consistent with protective immunity. These animals remained healthy after challenge inoculation with a lethal dose with homologous or heterologous <italic>wt</italic>
H5N1 highly pathogenic avian influenza (HPAI) viruses. The profiles of viral replication in respiratory tissues and the immunogenicity and protective efficacy characteristics of the two <italic>ca</italic>
H5N1 candidate LAIV viruses warrant further development into a vaccine for human use.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Pays-Bas</li>
<li>Russie</li>
<li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Isakova Sivak, Irina" sort="Isakova Sivak, Irina" uniqKey="Isakova Sivak I" first="Irina" last="Isakova-Sivak">Irina Isakova-Sivak</name>
</region>
<name sortKey="Bourgeois, Melissa" sort="Bourgeois, Melissa" uniqKey="Bourgeois M" first="Melissa" last="Bourgeois">Melissa Bourgeois</name>
<name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J." last="Cox">Nancy J. Cox</name>
<name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<name sortKey="Klimov, Alexander" sort="Klimov, Alexander" uniqKey="Klimov A" first="Alexander" last="Klimov">Alexander Klimov</name>
<name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
</country>
<country name="Russie"><noRegion><name sortKey="Isakova Sivak, Irina" sort="Isakova Sivak, Irina" uniqKey="Isakova Sivak I" first="Irina" last="Isakova-Sivak">Irina Isakova-Sivak</name>
</noRegion>
<name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name>
</country>
<country name="Pays-Bas"><noRegion><name sortKey="Voeten, J Theo M" sort="Voeten, J Theo M" uniqKey="Voeten J" first="J. Theo M." last="Voeten">J. Theo M. Voeten</name>
</noRegion>
<name sortKey="Heldens, Jacco G M" sort="Heldens, Jacco G M" uniqKey="Heldens J" first="Jacco G. M." last="Heldens">Jacco G. M. Heldens</name>
<name sortKey="Van Den Bosch, Han" sort="Van Den Bosch, Han" uniqKey="Van Den Bosch H" first="Han" last="Van Den Bosch">Han Van Den Bosch</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000528 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000528 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:4018889 |texte= Characterization of Reverse Genetics-Derived Cold-Adapted Master Donor Virus A/Leningrad/134/17/57 (H2N2) and Reassortants with H5N1 Surface Genes in a Mouse Model }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:24648485" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |