North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
Identifieur interne : 000483 ( Pmc/Checkpoint ); précédent : 000482; suivant : 000484North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
Auteurs : Wenjun Ma ; Qinfang Liu ; Chuanling Qiao ; Gustavo Del Real ; Adolfo García-Sastre ; Richard J. Webby ; Jürgen A. RichtSource :
- mBio [ 2150-7511 ] ; 2014.
Abstract
The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment.
Url:
DOI: 10.1128/mBio.00919-13
PubMed: 24618255
PubMed Central: 3952159
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus</title>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment.</p>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">mBio</journal-id>
<journal-id journal-id-type="iso-abbrev">MBio</journal-id>
<journal-id journal-id-type="hwp">mbio</journal-id>
<journal-id journal-id-type="pmc">mbio</journal-id>
<journal-id journal-id-type="publisher-id">mBio</journal-id>
<journal-title-group><journal-title>mBio</journal-title>
</journal-title-group>
<issn pub-type="epub">2150-7511</issn>
<publisher><publisher-name>American Society of Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24618255</article-id>
<article-id pub-id-type="pmc">3952159</article-id>
<article-id pub-id-type="publisher-id">mBio00919-13</article-id>
<article-id pub-id-type="doi">10.1128/mBio.00919-13</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus</article-title>
<alt-title>Reassortment of North American and Eurasian H1N1 SIVs</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Ma</surname>
<given-names>Wenjun</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
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</contrib>
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<given-names>Qinfang</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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</contrib>
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<given-names>Chuanling</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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<xref ref-type="author-notes" rid="presentAddress1">*</xref>
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<contrib contrib-type="author"><name><surname>del Real</surname>
<given-names>Gustavo</given-names>
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<xref ref-type="aff" rid="aff2"><sup>b</sup>
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<given-names>Adolfo</given-names>
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<xref ref-type="aff" rid="aff3 aff4 aff5"><sup>c,d,e</sup>
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<given-names>Richard J.</given-names>
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<xref ref-type="aff" rid="aff6"><sup>f</sup>
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<given-names>Jürgen A.</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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</contrib>
<aff id="aff1"><label><sup>a</sup>
</label>
Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA</aff>
<aff id="aff2"><label><sup>b</sup>
</label>
Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain</aff>
<aff id="aff3"><label><sup>c</sup>
</label>
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</aff>
<aff id="aff4"><label><sup>d</sup>
</label>
Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA</aff>
<aff id="aff5"><label><sup>e</sup>
</label>
Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA</aff>
<aff id="aff6"><label><sup>f</sup>
</label>
St. Jude Children’s Research Hospital, Memphis, Tennessee, USA</aff>
</contrib-group>
<author-notes><corresp id="cor2">Address correspondence to Wenjun Ma, <email>wma@vet.k-state.edu</email>
, or Juergen Richt, <email>jricht@vet.k-state.edu</email>
.</corresp>
<fn id="presentAddress1" fn-type="present-address"><label>*</label>
<p>Present address: Harbin Veterinary Research Institute, Harbin, China.</p>
</fn>
<fn fn-type="edited-by"><p><bold>Invited Editor</bold>
Joseph Sriyal Peiris, The University of Hong Kong <bold>Editor</bold>
W. Ian Lipkin, Columbia University</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>11</day>
<month>3</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="collection"><season>Mar-Apr</season>
<year>2014</year>
</pub-date>
<volume>5</volume>
<issue>2</issue>
<elocation-id>e00919-13</elocation-id>
<history><date date-type="received"><day>25</day>
<month>10</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>23</day>
<month>1</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014 Ma et al.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>Ma et al.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/"><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license</ext-link>
, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><title>ABSTRACT</title>
<p>The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment.</p>
</abstract>
<abstract abstract-type="executive-summary"><title>IMPORTANCE</title>
<p>The 2009 pandemic-like H1N1 virus could not be reproduced either in cell cultures or in pigs coinfected with North American triple reassortant H1N1 and Eurasian H1N1 swine influenza viruses. This finding suggests that the generation of the 2009 pandemic H1N1 virus by reassortment was a rare event that likely involved specific viral strains and unknown factors. Different reassortant viruses were detected in coinfected pigs with and without preexisting immunity, indicating that host immunity plays a relevant role in driving viral reassortment of influenza A virus.</p>
</abstract>
<counts><page-count count="9"></page-count>
</counts>
<custom-meta-group><custom-meta><meta-name>cover-date</meta-name>
<meta-value>March/April 2014</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Del Real, Gustavo" sort="Del Real, Gustavo" uniqKey="Del Real G" first="Gustavo" last="Del Real">Gustavo Del Real</name>
<name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name>
<name sortKey="Liu, Qinfang" sort="Liu, Qinfang" uniqKey="Liu Q" first="Qinfang" last="Liu">Qinfang Liu</name>
<name sortKey="Ma, Wenjun" sort="Ma, Wenjun" uniqKey="Ma W" first="Wenjun" last="Ma">Wenjun Ma</name>
<name sortKey="Qiao, Chuanling" sort="Qiao, Chuanling" uniqKey="Qiao C" first="Chuanling" last="Qiao">Chuanling Qiao</name>
<name sortKey="Richt, Jurgen A" sort="Richt, Jurgen A" uniqKey="Richt J" first="Jürgen A." last="Richt">Jürgen A. Richt</name>
<name sortKey="Webby, Richard J" sort="Webby, Richard J" uniqKey="Webby R" first="Richard J." last="Webby">Richard J. Webby</name>
</noCountry>
</tree>
</affiliations>
</record>
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