Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers
Identifieur interne : 000364 ( Pmc/Checkpoint ); précédent : 000363; suivant : 000365Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers
Auteurs : Yulia A. Desheva [Russie] ; Tatiana A. Smolonogina [Russie] ; Svetlana A. Donina [Russie] ; Larisa G. Rudenko [Russie]Source :
- BMC Research Notes [ 1756-0500 ] ; 2015.
Abstract
Pre-existing antibodies to influenza virus neuraminidase may provide protection against infection influenza viruses containing novel hemagglutinin (HA). The aim of our study was to evaluate serum neuraminidase-inhibiting (NI) antibodies against А/California/07/2009(H1N1) [H1N1/2009pdm] and А/New Caledonia/20/1999(H1N1) [H1N1/1999] influenza viruses in relation with the age of participants and hemagglutination-inhibition (HI) antibody levels. Anti-H1N1/2009pdm neuraminidase and anti-H1N1/1999 neuraminidase antibody levels were measured in total 219 serum samples from Russian healthy peoples of various ages examined before and a year after pandemic strain appearance. We adjusted peroxidase-linked lectin micro-procedure to measure NI antibody titers using the reassortant A/H7N1 influenza viruses based on A/equine/Prague/1/56(H7N7). Also, HI antibody titers were estimated against H1N1/2009pdm, H1N1/1999 and a panel of seasonal A/H1N1 influenza viruses.
In sera samples collected during the fall of 2010, mean titers of specific HI and NI antibodies to H1N1/2009pdm were 2–2.1 times lower than antibody levels against H1N1/1999. Of the 163 individuals examined, 58 (35.6%) had NI anti-H1N1/2009pdm antibody titers > 1:20, compared to 93 (57.1%) who had NI anti-H1N1/1999 antibody titers > 1:20. There were low correlations between HI and NI antibody levels against either H1N1/1999 or H1N1/2009pdm in the same serum samples. The 24 adults born between 1957 and 1977 expressed very low levels of NI antibodies to A/H1N1 influenza viruses. Persons with low HI anti-H1N1/2009pdm titers but positive to seasonal A/H1N1 demonstrated significantly higher NI anti-A/H1N1 antibody titers than unexposed subjects. In 2005 cross-reactive NI anti-H1N1/2009pdm antibody titers > 1:20 were detected among 7.1% of young people.
Our study confirmed that contact with seasonal influenza viruses may have contributed to generating the cross-reacting anti-H1N1/2009pdm NI antibodies which were detected in the sera of 18-20 years old people examined before the pandemic virus active circulation. The lowest levels of antibodies to the neuraminidase of N1 subtype were in the group of participants born during the circulation of influenza A/H2N2 or A/H3N2 viruses. The low correlation between HI and NI antibody titers suggests that NI antibody detection can be used as an additional test to evaluate the immune response after influenza infections or immunizations.
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DOI: 10.1186/s13104-015-1086-z
PubMed: 25889924
PubMed Central: 4399204
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers</title><author><name sortKey="Desheva, Yulia A" sort="Desheva, Yulia A" uniqKey="Desheva Y" first="Yulia A" last="Desheva">Yulia A. Desheva</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Smolonogina, Tatiana A" sort="Smolonogina, Tatiana A" uniqKey="Smolonogina T" first="Tatiana A" last="Smolonogina">Tatiana A. Smolonogina</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Donina, Svetlana A" sort="Donina, Svetlana A" uniqKey="Donina S" first="Svetlana A" last="Donina">Svetlana A. Donina</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Rudenko, Larisa G" sort="Rudenko, Larisa G" uniqKey="Rudenko L" first="Larisa G" last="Rudenko">Larisa G. Rudenko</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25889924</idno><idno type="pmc">4399204</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399204</idno><idno type="RBID">PMC:4399204</idno><idno type="doi">10.1186/s13104-015-1086-z</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000169</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000169</idno><idno type="wicri:Area/Pmc/Curation">000169</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000169</idno><idno type="wicri:Area/Pmc/Checkpoint">000364</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000364</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers</title><author><name sortKey="Desheva, Yulia A" sort="Desheva, Yulia A" uniqKey="Desheva Y" first="Yulia A" last="Desheva">Yulia A. Desheva</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Smolonogina, Tatiana A" sort="Smolonogina, Tatiana A" uniqKey="Smolonogina T" first="Tatiana A" last="Smolonogina">Tatiana A. Smolonogina</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Donina, Svetlana A" sort="Donina, Svetlana A" uniqKey="Donina S" first="Svetlana A" last="Donina">Svetlana A. Donina</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Rudenko, Larisa G" sort="Rudenko, Larisa G" uniqKey="Rudenko L" first="Larisa G" last="Rudenko">Larisa G. Rudenko</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Research Notes</title><idno type="eISSN">1756-0500</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Pre-existing antibodies to influenza virus neuraminidase may provide protection against infection influenza viruses containing novel hemagglutinin (HA). The aim of our study was to evaluate serum neuraminidase-inhibiting (NI) antibodies against А/California/07/2009(H1N1) [H1N1/2009pdm] and А/New Caledonia/20/1999(H1N1) [H1N1/1999] influenza viruses in relation with the age of participants and hemagglutination-inhibition (HI) antibody levels. Anti-H1N1/2009pdm neuraminidase and anti-H1N1/1999 neuraminidase antibody levels were measured in total 219 serum samples from Russian healthy peoples of various ages examined before and a year after pandemic strain appearance. We adjusted peroxidase-linked lectin micro-procedure to measure NI antibody titers using the reassortant A/H7N1 influenza viruses based on A/equine/Prague/1/56(H7N7). Also, HI antibody titers were estimated against H1N1/2009pdm, H1N1/1999 and a panel of seasonal A/H1N1 influenza viruses.</p></sec><sec><title>Results</title><p>In sera samples collected during the fall of 2010, mean titers of specific HI and NI antibodies to H1N1/2009pdm were 2–2.1 times lower than antibody levels against H1N1/1999. Of the 163 individuals examined, 58 (35.6%) had NI anti-H1N1/2009pdm antibody titers > 1:20, compared to 93 (57.1%) who had NI anti-H1N1/1999 antibody titers > 1:20. There were low correlations between HI and NI antibody levels against either H1N1/1999 or H1N1/2009pdm in the same serum samples. The 24 adults born between 1957 and 1977 expressed very low levels of NI antibodies to A/H1N1 influenza viruses. Persons with low HI anti-H1N1/2009pdm titers but positive to seasonal A/H1N1 demonstrated significantly higher NI anti-A/H1N1 antibody titers than unexposed subjects. In 2005 cross-reactive NI anti-H1N1/2009pdm antibody titers > 1:20 were detected among 7.1% of young people.</p></sec><sec><title>Conclusions</title><p>Our study confirmed that contact with seasonal influenza viruses may have contributed to generating the cross-reacting anti-H1N1/2009pdm NI antibodies which were detected in the sera of 18-20 years old people examined before the pandemic virus active circulation. The lowest levels of antibodies to the neuraminidase of N1 subtype were in the group of participants born during the circulation of influenza A/H2N2 or A/H3N2 viruses. The low correlation between HI and NI antibody titers suggests that NI antibody detection can be used as an additional test to evaluate the immune response after influenza infections or immunizations.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Garten, Rj" uniqKey="Garten R">RJ Garten</name></author><author><name sortKey="Davis, Ct" uniqKey="Davis C">CT Davis</name></author><author><name sortKey="Russell, Ca" uniqKey="Russell C">CA Russell</name></author><author><name sortKey="Shu, B" uniqKey="Shu B">B Shu</name></author><author><name sortKey="Lindstrom, S" uniqKey="Lindstrom S">S Lindstrom</name></author><author><name sortKey="Balish, A" uniqKey="Balish A">A Balish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hancock, K" uniqKey="Hancock K">K Hancock</name></author><author><name sortKey="Veguilla, V" uniqKey="Veguilla V">V Veguilla</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author><author><name sortKey="Zhong, W" uniqKey="Zhong W">W Zhong</name></author><author><name sortKey="Butler, En" uniqKey="Butler E">EN Butler</name></author><author><name sortKey="Sun, H" uniqKey="Sun H">H Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenbaum, Ja" uniqKey="Greenbaum J">JA Greenbaum</name></author><author><name sortKey="Kotturi, Mf" uniqKey="Kotturi M">MF Kotturi</name></author><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y Kim</name></author><author><name sortKey="Oseroff, C" uniqKey="Oseroff C">C Oseroff</name></author><author><name sortKey="Vaughan, K" uniqKey="Vaughan K">K Vaughan</name></author><author><name sortKey="Salimi, N" uniqKey="Salimi N">N Salimi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name></author><author><name sortKey="Bodewes, R" uniqKey="Bodewes R">R Bodewes</name></author><author><name sortKey="De Vries, Rp" uniqKey="De Vries R">RP de Vries</name></author><author><name sortKey="Kreijtz, Jh" uniqKey="Kreijtz J">JH Kreijtz</name></author><author><name sortKey="Bartelink, W" uniqKey="Bartelink W">W Bartelink</name></author><author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G van Amerongen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johansson, Be" uniqKey="Johansson B">BE Johansson</name></author><author><name sortKey="Grajower, B" uniqKey="Grajower B">B Grajower</name></author><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sandbulte, Mr" uniqKey="Sandbulte M">MR Sandbulte</name></author><author><name sortKey="Jimenez, Gs" uniqKey="Jimenez G">GS Jimenez</name></author><author><name sortKey="Boon, Ac" uniqKey="Boon A">AC Boon</name></author><author><name sortKey="Smith, Lr" uniqKey="Smith L">LR Smith</name></author><author><name sortKey="Treanor, Jj" uniqKey="Treanor J">JJ Treanor</name></author><author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schulman, Jl" uniqKey="Schulman J">JL Schulman</name></author><author><name sortKey="Khakpour, M" uniqKey="Khakpour M">M Khakpour</name></author><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monto, As" uniqKey="Monto A">AS Monto</name></author><author><name sortKey="Kendal, Ap" uniqKey="Kendal A">AP Kendal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cate, Tr" uniqKey="Cate T">TR Cate</name></author><author><name sortKey="Rayford, Y" uniqKey="Rayford Y">Y Rayford</name></author><author><name sortKey="Nino, D" uniqKey="Nino D">D Nino</name></author><author><name sortKey="Winokur, P" uniqKey="Winokur P">P Winokur</name></author><author><name sortKey="Brady, R" uniqKey="Brady R">R Brady</name></author><author><name sortKey="Belshe, R" uniqKey="Belshe R">R Belshe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marcelin, G" uniqKey="Marcelin G">G Marcelin</name></author><author><name sortKey="Bland, Hm" uniqKey="Bland H">HM Bland</name></author><author><name sortKey="Negovetich, Nj" uniqKey="Negovetich N">NJ Negovetich</name></author><author><name sortKey="Sandbulte, Mr" uniqKey="Sandbulte M">MR Sandbulte</name></author><author><name sortKey="Ellebedy, Ah" uniqKey="Ellebedy A">AH Ellebedy</name></author><author><name sortKey="Webb, Ad" uniqKey="Webb A">AD Webb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nauta, Jjp" uniqKey="Nauta J">JJP Nauta</name></author><author><name sortKey="Beyer, Wep" uniqKey="Beyer W">WEP Beyer</name></author><author><name sortKey="Osterhaus, Adme" uniqKey="Osterhaus A">ADME Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cramer, J" uniqKey="Cramer J">J Cramer</name></author><author><name sortKey="Mac, T" uniqKey="Mac T">T Mac</name></author><author><name sortKey="Hogan, B" uniqKey="Hogan B">B Hogan</name></author><author><name sortKey="Stauga, S" uniqKey="Stauga S">S Stauga</name></author><author><name sortKey="Eberhardt, S" uniqKey="Eberhardt S">S Eberhardt</name></author><author><name sortKey="Wichmann, O" uniqKey="Wichmann O">O Wichmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Broberg, E" uniqKey="Broberg E">E Broberg</name></author><author><name sortKey="Nicoll, A" uniqKey="Nicoll A">A Nicoll</name></author><author><name sortKey="Amato Gauci, A" uniqKey="Amato Gauci A">A Amato-Gauci</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jennings, R" uniqKey="Jennings R">R Jennings</name></author><author><name sortKey="Potter, Cw" uniqKey="Potter C">CW Potter</name></author><author><name sortKey="Massey, Pm" uniqKey="Massey P">PM Massey</name></author><author><name sortKey="Duerden, Bi" uniqKey="Duerden B">BI Duerden</name></author><author><name sortKey="Martin, J" uniqKey="Martin J">J Martin</name></author><author><name sortKey="Bevan, Am" uniqKey="Bevan A">AM Bevan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kendal, Ap" uniqKey="Kendal A">AP Kendal</name></author><author><name sortKey="Bozeman, Fm" uniqKey="Bozeman F">FM Bozeman</name></author><author><name sortKey="Ennis, Fa" uniqKey="Ennis F">FA Ennis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schild, Gc" uniqKey="Schild G">GC Schild</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, Aj" uniqKey="Smith A">AJ Smith</name></author><author><name sortKey="Davies, Jr" uniqKey="Davies J">JR Davies</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author><author><name sortKey="Kasel, Ja" uniqKey="Kasel J">JA Kasel</name></author><author><name sortKey="Chanock, Rm" uniqKey="Chanock R">RM Chanock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Desheva, Y" uniqKey="Desheva Y">Y Desheva</name></author><author><name sortKey="Smolonogina, T" uniqKey="Smolonogina T">T Smolonogina</name></author><author><name sortKey="Rudenko, L" uniqKey="Rudenko L">L Rudenko</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Lambre, Cr" uniqKey="Lambre C">CR Lambré</name></author><author><name sortKey="Terzidis, H" uniqKey="Terzidis H">H Terzidis</name></author><author><name sortKey="Greffard, A" uniqKey="Greffard A">A Greffard</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Res Notes</journal-id><journal-id journal-id-type="iso-abbrev">BMC Res Notes</journal-id><journal-title-group><journal-title>BMC Research Notes</journal-title></journal-title-group><issn pub-type="epub">1756-0500</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25889924</article-id><article-id pub-id-type="pmc">4399204</article-id><article-id pub-id-type="publisher-id">1086</article-id><article-id pub-id-type="doi">10.1186/s13104-015-1086-z</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Serum strain-specific or cross-reactive neuraminidase inhibiting antibodies against pandemic А/California/07/2009(H1N1) influenza in healthy volunteers</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Desheva</surname><given-names>Yulia A</given-names></name><address><email>desheva@mail.ru</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Smolonogina</surname><given-names>Tatiana A</given-names></name><address><email>smolonogina@mail.ru</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Donina</surname><given-names>Svetlana A</given-names></name><address><email>sveta.donina@gmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Rudenko</surname><given-names>Larisa G</given-names></name><address><email>vaccine@mail.ru</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1">Institute of Experimental Medicine, Russian Academy of the Medical Sciences, Acad. Pavlov’s Street, 12, Saint Petersburg, 197376 Russian Federation</aff></contrib-group><pub-date pub-type="epub"><day>10</day><month>4</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>10</day><month>4</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>8</volume><elocation-id>136</elocation-id><history><date date-type="received"><day>6</day><month>12</month><year>2014</year></date><date date-type="accepted"><day>23</day><month>3</month><year>2015</year></date></history><permissions><copyright-statement>© Desheva et al.; licensee BioMed Central. 2015</copyright-statement><license license-type="open-access"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Pre-existing antibodies to influenza virus neuraminidase may provide protection against infection influenza viruses containing novel hemagglutinin (HA). The aim of our study was to evaluate serum neuraminidase-inhibiting (NI) antibodies against А/California/07/2009(H1N1) [H1N1/2009pdm] and А/New Caledonia/20/1999(H1N1) [H1N1/1999] influenza viruses in relation with the age of participants and hemagglutination-inhibition (HI) antibody levels. Anti-H1N1/2009pdm neuraminidase and anti-H1N1/1999 neuraminidase antibody levels were measured in total 219 serum samples from Russian healthy peoples of various ages examined before and a year after pandemic strain appearance. We adjusted peroxidase-linked lectin micro-procedure to measure NI antibody titers using the reassortant A/H7N1 influenza viruses based on A/equine/Prague/1/56(H7N7). Also, HI antibody titers were estimated against H1N1/2009pdm, H1N1/1999 and a panel of seasonal A/H1N1 influenza viruses.</p></sec><sec><title>Results</title><p>In sera samples collected during the fall of 2010, mean titers of specific HI and NI antibodies to H1N1/2009pdm were 2–2.1 times lower than antibody levels against H1N1/1999. Of the 163 individuals examined, 58 (35.6%) had NI anti-H1N1/2009pdm antibody titers > 1:20, compared to 93 (57.1%) who had NI anti-H1N1/1999 antibody titers > 1:20. There were low correlations between HI and NI antibody levels against either H1N1/1999 or H1N1/2009pdm in the same serum samples. The 24 adults born between 1957 and 1977 expressed very low levels of NI antibodies to A/H1N1 influenza viruses. Persons with low HI anti-H1N1/2009pdm titers but positive to seasonal A/H1N1 demonstrated significantly higher NI anti-A/H1N1 antibody titers than unexposed subjects. In 2005 cross-reactive NI anti-H1N1/2009pdm antibody titers > 1:20 were detected among 7.1% of young people.</p></sec><sec><title>Conclusions</title><p>Our study confirmed that contact with seasonal influenza viruses may have contributed to generating the cross-reacting anti-H1N1/2009pdm NI antibodies which were detected in the sera of 18-20 years old people examined before the pandemic virus active circulation. The lowest levels of antibodies to the neuraminidase of N1 subtype were in the group of participants born during the circulation of influenza A/H2N2 or A/H3N2 viruses. The low correlation between HI and NI antibody titers suggests that NI antibody detection can be used as an additional test to evaluate the immune response after influenza infections or immunizations.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Influenza virus</kwd><kwd>Neuraminidase</kwd><kwd>Anti-neuraminidase antibodies</kwd><kwd>Pandemic influenza</kwd><kwd>A/H1N1</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2015</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Desheva, Yulia A" sort="Desheva, Yulia A" uniqKey="Desheva Y" first="Yulia A" last="Desheva">Yulia A. Desheva</name></noRegion><name sortKey="Donina, Svetlana A" sort="Donina, Svetlana A" uniqKey="Donina S" first="Svetlana A" last="Donina">Svetlana A. Donina</name><name sortKey="Rudenko, Larisa G" sort="Rudenko, Larisa G" uniqKey="Rudenko L" first="Larisa G" last="Rudenko">Larisa G. Rudenko</name><name sortKey="Smolonogina, Tatiana A" sort="Smolonogina, Tatiana A" uniqKey="Smolonogina T" first="Tatiana A" last="Smolonogina">Tatiana A. Smolonogina</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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