Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses
Identifieur interne : 000070 ( PascalFrancis/Curation ); précédent : 000069; suivant : 000071Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses
Auteurs : Jens C. Krause [États-Unis] ; Tshidi Tsibane [États-Unis] ; Terrence M. Tumpey [États-Unis] ; Chelsey J. Huffman [États-Unis] ; Randy Albrecht [États-Unis] ; David L. Blum [États-Unis] ; Irene Ramos [États-Unis] ; Ana Fernandez-Sesma [États-Unis] ; Kathryn M. Edwards [États-Unis] ; Adolfo Garcia-Sastre [États-Unis] ; Christopher F. Basler [États-Unis] ; James E. Jr Crowe [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born between 1950 and 1968. Two MAbs reacted with the pandemic H2N2 virus, two recognized the pandemic H3N2 virus, and remarkably, one reacted with both the pandemic H2N2 and H3N2 viruses. Each of these five naturally occurring MAbs displayed hemagglutination inhibition activity, suggesting specificity for the globular head domain of influenza virus HA. When incubated with virus, MAbs 8F8, 8M2, and 2G1 each elicited H2N2 escape mutations immediately adjacent to the receptor-binding domain on the HA globular head in embryonated chicken eggs. All H2N2-specific MAbs were able to inhibit a 2006 swine H2N3 influenza virus. MAbs 8M2 and 2G1 shared the VH1-69 germ line gene, but these antibodies were otherwise not genetically related. Each antibody was able to protect mice in a lethal H2N2 virus challenge. Thus, even 43 years after circulation of H2N2 viruses, these subjects possessed peripheral blood B cells encoding potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2 HA.
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses</title>
<author><name sortKey="Krause, Jens C" sort="Krause, Jens C" uniqKey="Krause J" first="Jens C." last="Krause">Jens C. Krause</name>
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<author><name sortKey="Albrecht, Randy" sort="Albrecht, Randy" uniqKey="Albrecht R" first="Randy" last="Albrecht">Randy Albrecht</name>
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<author><name sortKey="Blum, David L" sort="Blum, David L" uniqKey="Blum D" first="David L." last="Blum">David L. Blum</name>
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<author><name sortKey="Fernandez Sesma, Ana" sort="Fernandez Sesma, Ana" uniqKey="Fernandez Sesma A" first="Ana" last="Fernandez-Sesma">Ana Fernandez-Sesma</name>
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<author><name sortKey="Edwards, Kathryn M" sort="Edwards, Kathryn M" uniqKey="Edwards K" first="Kathryn M." last="Edwards">Kathryn M. Edwards</name>
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<author><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="Garcia-Sastre">Adolfo Garcia-Sastre</name>
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<s2>New York, New York</s2>
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<s2>New York, New York</s2>
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<author><name sortKey="Crowe, James E Jr" sort="Crowe, James E Jr" uniqKey="Crowe J" first="James E. Jr" last="Crowe">James E. Jr Crowe</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pediatrics, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
<s3>USA</s3>
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<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Microbiology and Immunology, Vanderbilt University Medical Center</s1>
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<series><title level="j" type="main">Journal of virology</title>
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<front><div type="abstract" xml:lang="en">Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born between 1950 and 1968. Two MAbs reacted with the pandemic H2N2 virus, two recognized the pandemic H3N2 virus, and remarkably, one reacted with both the pandemic H2N2 and H3N2 viruses. Each of these five naturally occurring MAbs displayed hemagglutination inhibition activity, suggesting specificity for the globular head domain of influenza virus HA. When incubated with virus, MAbs 8F8, 8M2, and 2G1 each elicited H2N2 escape mutations immediately adjacent to the receptor-binding domain on the HA globular head in embryonated chicken eggs. All H2N2-specific MAbs were able to inhibit a 2006 swine H2N3 influenza virus. MAbs 8M2 and 2G1 shared the V<sub>H</sub>
1-69 germ line gene, but these antibodies were otherwise not genetically related. Each antibody was able to protect mice in a lethal H2N2 virus challenge. Thus, even 43 years after circulation of H2N2 viruses, these subjects possessed peripheral blood B cells encoding potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2 HA.</div>
</front>
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<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0>
</fA01>
<fA03 i2="1"><s0>J. virol.</s0>
</fA03>
<fA05><s2>86</s2>
</fA05>
<fA06><s2>11</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>KRAUSE (Jens C.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>TSIBANE (Tshidi)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>TUMPEY (Terrence M.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>HUFFMAN (Chelsey J.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>ALBRECHT (Randy)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BLUM (David L.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>RAMOS (Irene)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>FERNANDEZ-SESMA (Ana)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>EDWARDS (Kathryn M.)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>GARCIA-SASTRE (Adolfo)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>BASLER (Christopher F.)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>CROWE (James E. JR)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Pediatrics, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Microbiology and Immunology, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
<s3>USA</s3>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Microbiology, Mount Sinai School of Medicine</s1>
<s2>New York, New York</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine</s1>
<s2>New York, New York</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine</s1>
<s2>New York, New York</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Influenza Division, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA20><s1>6334-6340</s1>
</fA20>
<fA21><s1>2012</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
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<s1>© 2012 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>54 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>12-0227810</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born between 1950 and 1968. Two MAbs reacted with the pandemic H2N2 virus, two recognized the pandemic H3N2 virus, and remarkably, one reacted with both the pandemic H2N2 and H3N2 viruses. Each of these five naturally occurring MAbs displayed hemagglutination inhibition activity, suggesting specificity for the globular head domain of influenza virus HA. When incubated with virus, MAbs 8F8, 8M2, and 2G1 each elicited H2N2 escape mutations immediately adjacent to the receptor-binding domain on the HA globular head in embryonated chicken eggs. All H2N2-specific MAbs were able to inhibit a 2006 swine H2N3 influenza virus. MAbs 8M2 and 2G1 shared the V<sub>H</sub>
1-69 germ line gene, but these antibodies were otherwise not genetically related. Each antibody was able to protect mice in a lethal H2N2 virus challenge. Thus, even 43 years after circulation of H2N2 viruses, these subjects possessed peripheral blood B cells encoding potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2 HA.</s0>
</fC01>
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</fC02>
<fC02 i1="02" i2="X"><s0>002A05C07</s0>
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<s5>01</s5>
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<s5>01</s5>
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<fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0>
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<fN21><s1>177</s1>
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