H2N2V1, PascalFrancis, Corpus, bibRecord, 000013

A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

Identifieur interne : 000013 ( PascalFrancis/Corpus ); précédent : 000012; suivant : 000014

A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

Auteurs : DUJUAN LI ; JIANPING WANG ; RONGHUI WANG ; YANBIN LI ; Daad Abi-Ghanem ; Luc Berghman ; Billy Hargis ; HUAGUANG LU

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2011.

RBID : Pascal:11-0290321

Descripteurs français

Pascal (Inist)
- Microbalance quartz, Immunodétecteur, Détection, Grippe aviaire, Magnétique, Influenzavirus aviaire, Cristal, Anticorps, Souche H5N1, Influenzavirus A(H5N1).

English descriptors

KwdEn :
- Antibody, Avian influenza, Avian influenzavirus, Crystals, Detection, H5N1, Immunosensor, Influenzavirus A(H5N1), Magnetic, Quartz microbalance.

Abstract

As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 11-0290321 INIST
ET :	A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1
AU :	DUJUAN LI; JIANPING WANG; RONGHUI WANG; YANBIN LI; ABI-GHANEM (Daad); BERGHMAN (Luc); HARGIS (Billy); HUAGUANG LU
AF :	College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road/Hangzhou 310058/Chine (1 aut., 2 aut.); Department of Biological and Agricultural Engineering, University of Arkansas/Fayetteville, AR 72701/Etats-Unis (1 aut., 3 aut., 4 aut.); Center of Excellence for Poultry Science, University of Arkansas/Fayetteville, AR 72701/Etats-Unis (4 aut., 7 aut.); Department of Poultry Science and Veterinary Pathobiology, Texas A&M University/College Station, TX 77843/Etats-Unis (5 aut., 6 aut.); Animal Diagnostic Laboratory, Penn State University/State College, PA 16802/Etats-Unis (8 aut.)
DT :	Publication en série; Niveau analytique
SO :	Biosensors & bioelectronics; ISSN 0956-5663; Royaume-Uni; Da. 2011; Vol. 26; No. 10; Pp. 4146-4154; Bibl. 3/4 p.
LA :	Anglais
EA :	As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.
CC :	002A31C09B; 215
FD :	Microbalance quartz; Immunodétecteur; Détection; Grippe aviaire; Magnétique; Influenzavirus aviaire; Cristal; Anticorps; Souche H5N1; Influenzavirus A(H5N1)
FG :	Biodétecteur; Virose; Infection; Influenzavirus A; Orthomyxoviridae; Virus
ED :	Quartz microbalance; Immunosensor; Detection; Avian influenza; Magnetic; Avian influenzavirus; Crystals; Antibody; H5N1; Influenzavirus A(H5N1)
EG :	Biosensor; Viral disease; Infection; Influenzavirus A; Orthomyxoviridae; Virus
SD :	Microbalanza cuarzo; Inmunodetector; Detección; Gripe aviar; Magnético; Avian influenzavirus; Cristal; Anticuerpo
LO :	INIST-20668.354000190343220270
ID :	11-0290321

Links to Exploration step

Pascal:11-0290321

Le document en format XML

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<front><div type="abstract" xml:lang="en">As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.</div>
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<fA08 i1="01" i2="1" l="ENG"><s1>A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>DUJUAN LI</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>JIANPING WANG</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>RONGHUI WANG</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>YANBIN LI</s1>
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<fA11 i1="05" i2="1"><s1>ABI-GHANEM (Daad)</s1>
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<fA11 i1="06" i2="1"><s1>BERGHMAN (Luc)</s1>
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<fA11 i1="07" i2="1"><s1>HARGIS (Billy)</s1>
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<fA11 i1="08" i2="1"><s1>HUAGUANG LU</s1>
</fA11>
<fA14 i1="01"><s1>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road</s1>
<s2>Hangzhou 310058</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Biological and Agricultural Engineering, University of Arkansas</s1>
<s2>Fayetteville, AR 72701</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Center of Excellence for Poultry Science, University of Arkansas</s1>
<s2>Fayetteville, AR 72701</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Poultry Science and Veterinary Pathobiology, Texas A&M University</s1>
<s2>College Station, TX 77843</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
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<fA14 i1="05"><s1>Animal Diagnostic Laboratory, Penn State University</s1>
<s2>State College, PA 16802</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
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<fA64 i1="01" i2="1"><s0>Biosensors & bioelectronics</s0>
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<fC01 i1="01" l="ENG"><s0>As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.</s0>
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<s5>11</s5>
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<fN21><s1>192</s1>
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<server><NO>PASCAL 11-0290321 INIST</NO>
<ET>A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1</ET>
<AU>DUJUAN LI; JIANPING WANG; RONGHUI WANG; YANBIN LI; ABI-GHANEM (Daad); BERGHMAN (Luc); HARGIS (Billy); HUAGUANG LU</AU>
<AF>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road/Hangzhou 310058/Chine (1 aut., 2 aut.); Department of Biological and Agricultural Engineering, University of Arkansas/Fayetteville, AR 72701/Etats-Unis (1 aut., 3 aut., 4 aut.); Center of Excellence for Poultry Science, University of Arkansas/Fayetteville, AR 72701/Etats-Unis (4 aut., 7 aut.); Department of Poultry Science and Veterinary Pathobiology, Texas A&M University/College Station, TX 77843/Etats-Unis (5 aut., 6 aut.); Animal Diagnostic Laboratory, Penn State University/State College, PA 16802/Etats-Unis (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Biosensors & bioelectronics; ISSN 0956-5663; Royaume-Uni; Da. 2011; Vol. 26; No. 10; Pp. 4146-4154; Bibl. 3/4 p.</SO>
<LA>Anglais</LA>
<EA>As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.</EA>
<CC>002A31C09B; 215</CC>
<FD>Microbalance quartz; Immunodétecteur; Détection; Grippe aviaire; Magnétique; Influenzavirus aviaire; Cristal; Anticorps; Souche H5N1; Influenzavirus A(H5N1)</FD>
<FG>Biodétecteur; Virose; Infection; Influenzavirus A; Orthomyxoviridae; Virus</FG>
<ED>Quartz microbalance; Immunosensor; Detection; Avian influenza; Magnetic; Avian influenzavirus; Crystals; Antibody; H5N1; Influenzavirus A(H5N1)</ED>
<EG>Biosensor; Viral disease; Infection; Influenzavirus A; Orthomyxoviridae; Virus</EG>
<SD>Microbalanza cuarzo; Inmunodetector; Detección; Gripe aviar; Magnético; Avian influenzavirus; Cristal; Anticuerpo</SD>
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A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

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