H2N2V1, PascalFrancis, Checkpoint, bibRecord, 000088

Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion

Identifieur interne : 000088 ( PascalFrancis/Checkpoint ); précédent : 000087; suivant : 000089

Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion

Auteurs : S. Sakamoto [Japon] ; Y. Kino [Japon] ; T. Oka [Japon] ; M. L. Herlocher ; H. F. Maasab

Source :

Journal of virological methods [ 0166-0934 ] ; 1996.

RBID : Pascal:96-0165555

Descripteurs français

Pascal (Inist)
- Influenzavirus, Souche vaccinale, Souche atténuée, Virus recombinant, RNA, Organisation génome, Méthode analyse, Amplification génique, Réaction chaîne polymérase, Réassortiment génétique.

English descriptors

KwdEn :
- Analysis method, Attenuated strain, Gene amplification, Genetic reassortment, Genome organization, Influenzavirus, Polymerase chain reaction, RNA, Recombinant virus, Vaccine strain.

Abstract

An amplification system for nearly full length cDNA coding the eight influenza virus segments of A type (H1N1, H2N2, H3N2) and B type influenza viruses is described. Each of the segments of PB1, PB2, PA, NP, M, and NS can be amplified using one 5' primer and one 3' primer for A-type influenza viruses. The RT-PCR amplification system was applied to define the gene composition of three subtype cold-recombinant, live attenuated influenza viruses. Each segment of the attenuated influenza virus could be identified as deriving from segments of the Ca donor or wild virus by comparing the representative restriction enzyme digestion patterns of the three PCR products obtained from the Ca donor, the cold-live attenuated influenza viruses and the wild virus. This RT-PCR method, using RT-PCR followed by digestion of PCR products with restriction enzymes, was very beneficial for analyzing the genome of reassortant influenza viruses.

Affiliations:

Japon

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Pascal:96-0165555

Le document en format XML

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Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion

Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri