The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance
Identifieur interne : 000E43 ( Ncbi/Merge ); précédent : 000E42; suivant : 000E44The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance
Auteurs : Kathleen A. Stellrecht [États-Unis]Source :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2018.
Abstract
Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 years, subtype H3N2 viruses evolved to viral clades with mutations in the WHO-recommended target region, such that almost all isolates worldwide have significantly reduced sensitivities with many commercial reverse transcription-PCR tests.
Url:
DOI: 10.1128/JCM.01531-17
PubMed: 29305549
PubMed Central: 5824055
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PMC:5824055Le document en format XML
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 years, subtype H3N2 viruses evolved to viral clades with mutations in the WHO-recommended target region, such that almost all isolates worldwide have significantly reduced sensitivities with many commercial reverse transcription-PCR tests.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
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<title-group><article-title>The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance</article-title>
<alt-title alt-title-type="running-head">Minireview</alt-title>
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Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital and Albany Medical College, Albany, New York, USA</aff>
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<contrib-group><contrib contrib-type="editor"><name><surname>Kraft</surname>
<given-names>Colleen Suzanne</given-names>
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<aff>Emory University</aff>
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<author-notes><corresp id="cor1">Address correspondence to <email>stellrk@mail.amc.edu</email>
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<fn fn-type="other"><p><bold>Citation</bold>
Stellrecht KA. 2018. The drift in molecular testing for influenza: mutations affecting assay performance. J Clin Microbiol 56:e01531-17. <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.01531-17">https://doi.org/10.1128/JCM.01531-17</ext-link>
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<year>2018</year>
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<year>2018</year>
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<volume>56</volume>
<issue>3</issue>
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<permissions><copyright-statement>Copyright © 2018 American Society for Microbiology.</copyright-statement>
<copyright-year>2018</copyright-year>
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<abstract><title>ABSTRACT</title>
<p>Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 years, subtype H3N2 viruses evolved to viral clades with mutations in the WHO-recommended target region, such that almost all isolates worldwide have significantly reduced sensitivities with many commercial reverse transcription-PCR tests.</p>
</abstract>
<kwd-group><title>KEYWORDS</title>
<kwd>influenza</kwd>
<kwd>genetic drift</kwd>
<kwd>clades</kwd>
<kwd>diagnostics</kwd>
<kwd>RT-PCR</kwd>
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