Adjuvants and Immunization Strategies to Induce Influenza Virus Hemagglutinin Stalk Antibodies
Identifieur interne : 000980 ( Ncbi/Merge ); précédent : 000979; suivant : 000981Adjuvants and Immunization Strategies to Induce Influenza Virus Hemagglutinin Stalk Antibodies
Auteurs : Peter H. Goff [États-Unis] ; Dirk Eggink [États-Unis] ; Christopher W. Seibert [États-Unis] ; Rong Hai [États-Unis] ; Luis Martínez-Gil [États-Unis] ; Florian Krammer [États-Unis] ; Peter Palese [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.
Url:
DOI: 10.1371/journal.pone.0079194
PubMed: 24223176
PubMed Central: 3819267
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Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Smith, Gjd" uniqKey="Smith G">GJD Smith</name></author><author><name sortKey="Bahl, J" uniqKey="Bahl J">J Bahl</name></author><author><name sortKey="Vijaykrishna, D" uniqKey="Vijaykrishna D">D Vijaykrishna</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author><author><name sortKey="Shaw, M" uniqKey="Shaw M">M Shaw</name></author><author><name sortKey="Wright, P" uniqKey="Wright P">P Wright</name></author><author><name sortKey="Neumann, G" uniqKey="Neumann G">G Neumann</name></author><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24223176</article-id><article-id pub-id-type="pmc">3819267</article-id><article-id pub-id-type="publisher-id">PONE-D-13-16326</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0079194</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Adjuvants and Immunization Strategies to Induce Influenza Virus Hemagglutinin Stalk Antibodies</article-title><alt-title alt-title-type="running-head">Adjuvants and Hemagglutinin Stalk Antibodies</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Goff</surname><given-names>Peter H.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Eggink</surname><given-names>Dirk</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Seibert</surname><given-names>Christopher W.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hai</surname><given-names>Rong</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Martínez-Gil</surname><given-names>Luis</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Krammer</surname><given-names>Florian</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Palese</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Jiang</surname><given-names>Shibo</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Shanghai Medical College, Fudan University, China</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>peter.palese@mssm.edu</email></corresp><fn fn-type="COI-statement"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: PHG DE PP. Performed the experiments: PHG DE CWS. Analyzed the data: PHG DE PP. Contributed reagents/materials/analysis tools: PHG DE CWS RH LMG FK PP. Wrote the manuscript: PHG DE CWS RH LMG FK PP. </p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>6</day><month>11</month><year>2013</year></pub-date><volume>8</volume><issue>11</issue><elocation-id>e79194</elocation-id><history><date date-type="received"><day>22</day><month>4</month><year>2013</year></date><date date-type="accepted"><day>22</day><month>9</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 Goff et al</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Goff et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.</p></abstract><funding-group><funding-statement>This work was partially supported by a National Institutes of Health adjuvant development contract (HHSN272200900032C) and grant (1P01AI097092-353 01A1). Florian Krammer was supported by an Erwin Schrödinger fellowship (J 3232) from the Austrian Science Fund (FWF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Goff, Peter H" sort="Goff, Peter H" uniqKey="Goff P" first="Peter H." last="Goff">Peter H. Goff</name></region><name sortKey="Eggink, Dirk" sort="Eggink, Dirk" uniqKey="Eggink D" first="Dirk" last="Eggink">Dirk Eggink</name><name sortKey="Goff, Peter H" sort="Goff, Peter H" uniqKey="Goff P" first="Peter H." last="Goff">Peter H. Goff</name><name sortKey="Hai, Rong" sort="Hai, Rong" uniqKey="Hai R" first="Rong" last="Hai">Rong Hai</name><name sortKey="Krammer, Florian" sort="Krammer, Florian" uniqKey="Krammer F" first="Florian" last="Krammer">Florian Krammer</name><name sortKey="Martinez Gil, Luis" sort="Martinez Gil, Luis" uniqKey="Martinez Gil L" first="Luis" last="Martínez-Gil">Luis Martínez-Gil</name><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name><name sortKey="Seibert, Christopher W" sort="Seibert, Christopher W" uniqKey="Seibert C" first="Christopher W." last="Seibert">Christopher W. Seibert</name><name sortKey="Seibert, Christopher W" sort="Seibert, Christopher W" uniqKey="Seibert C" first="Christopher W." last="Seibert">Christopher W. Seibert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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