Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics
Identifieur interne : 000562 ( Ncbi/Merge ); précédent : 000561; suivant : 000563Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics
Auteurs : Giulia Campanini [Italie] ; Antonio Piralla [Italie] ; Stefania Paolucci [Italie] ; Francesca Rovida [Italie] ; Elena Percivalle [Italie] ; Giovanni Maga [Italie] ; Fausto Baldanti [Italie]Source :
- Virology Journal [ 1743-422X ] ; 2010.
Abstract
The Influenza A pandemic sustained by a new H1N1 variant (H1N1v) started in Mexico and the USA at the end of April 2009 spreading worldwide in a few weeks. In this study we investigate the variability of the NS1 gene of the pandemic H1N1v strain with respect to previous seasonal strains circulating in humans and the potential selection of virus variants through isolation in cell culture.
During the period April 27th 2009-Jan 15th 2010, 1633 potential 2009 H1N1v cases have been screened at our center using the CDC detection and typing realtime RT-PCR assays. Virus isolation on MDCK cells was systematically performed in 1/10 positive cases. A subset of 51 H1N1v strains isolated in the period May-September 2009 was selected for NS1 gene sequencing. In addition, 15 H1N1 and 47 H3N2 virus isolates from three previous seasonal epidemics (2006-2009) were analyzed in parallel.
A low variability in the NS1 amino acid (aa) sequence among H1N1v isolates was shown (aa identity 99.5%). A slightly higher NS1 variability was observed among H1N1 and H3N2 strains from previous epidemics (aa identity 98.6% and 98.9%, respectively). The H1N1v strains were closely related (aa identity 92.1%) to swine reference strain (A/swine/Oklahoma/042169/2008). In contrast, substantial divergence (aa identity 83.4%) with respect to human reference strain A/Brevig Mission/1/1918 and previous epidemic strains H1N1 and H3N2 (aa identity 78.9% and 77.6%, respectively) was shown. Specific sequence signatures of uncertain significance in the new virus variant were a C-terminus deletion and a T215P substitution.
The H1N1v NS1 gene was more conserved than that of previous epidemic strains. In addition, a closer genetic identity of H1N1v with the swine than the human reference strains was shown. Hot-spots were shown in the H1N1v NS1 aa sequence whose biologic relevance remains to be investigated.
Url:
DOI: 10.1186/1743-422X-7-209
PubMed: 20809948
PubMed Central: 2936903
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000C38
- to stream Pmc, to step Curation: 000C38
- to stream Pmc, to step Checkpoint: 000946
Links to Exploration step
PMC:2936903Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics</title><author><name sortKey="Campanini, Giulia" sort="Campanini, Giulia" uniqKey="Campanini G" first="Giulia" last="Campanini">Giulia Campanini</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Piralla, Antonio" sort="Piralla, Antonio" uniqKey="Piralla A" first="Antonio" last="Piralla">Antonio Piralla</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Paolucci, Stefania" sort="Paolucci, Stefania" uniqKey="Paolucci S" first="Stefania" last="Paolucci">Stefania Paolucci</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Rovida, Francesca" sort="Rovida, Francesca" uniqKey="Rovida F" first="Francesca" last="Rovida">Francesca Rovida</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Percivalle, Elena" sort="Percivalle, Elena" uniqKey="Percivalle E" first="Elena" last="Percivalle">Elena Percivalle</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Maga, Giovanni" sort="Maga, Giovanni" uniqKey="Maga G" first="Giovanni" last="Maga">Giovanni Maga</name><affiliation wicri:level="1"><nlm:aff id="I2">National Research Center IGBE CNR, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>National Research Center IGBE CNR, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Baldanti, Fausto" sort="Baldanti, Fausto" uniqKey="Baldanti F" first="Fausto" last="Baldanti">Fausto Baldanti</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20809948</idno><idno type="pmc">2936903</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936903</idno><idno type="RBID">PMC:2936903</idno><idno type="doi">10.1186/1743-422X-7-209</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000C38</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C38</idno><idno type="wicri:Area/Pmc/Curation">000C38</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C38</idno><idno type="wicri:Area/Pmc/Checkpoint">000946</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000946</idno><idno type="wicri:Area/Ncbi/Merge">000562</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics</title><author><name sortKey="Campanini, Giulia" sort="Campanini, Giulia" uniqKey="Campanini G" first="Giulia" last="Campanini">Giulia Campanini</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Piralla, Antonio" sort="Piralla, Antonio" uniqKey="Piralla A" first="Antonio" last="Piralla">Antonio Piralla</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Paolucci, Stefania" sort="Paolucci, Stefania" uniqKey="Paolucci S" first="Stefania" last="Paolucci">Stefania Paolucci</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Rovida, Francesca" sort="Rovida, Francesca" uniqKey="Rovida F" first="Francesca" last="Rovida">Francesca Rovida</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Percivalle, Elena" sort="Percivalle, Elena" uniqKey="Percivalle E" first="Elena" last="Percivalle">Elena Percivalle</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Maga, Giovanni" sort="Maga, Giovanni" uniqKey="Maga G" first="Giovanni" last="Maga">Giovanni Maga</name><affiliation wicri:level="1"><nlm:aff id="I2">National Research Center IGBE CNR, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>National Research Center IGBE CNR, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Baldanti, Fausto" sort="Baldanti, Fausto" uniqKey="Baldanti F" first="Fausto" last="Baldanti">Fausto Baldanti</name><affiliation wicri:level="1"><nlm:aff id="I1">Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The Influenza A pandemic sustained by a new H1N1 variant (H1N1v) started in Mexico and the USA at the end of April 2009 spreading worldwide in a few weeks. In this study we investigate the variability of the NS1 gene of the pandemic H1N1v strain with respect to previous seasonal strains circulating in humans and the potential selection of virus variants through isolation in cell culture.</p></sec><sec><title>Methods</title><p>During the period April 27<sup>th </sup>2009-Jan 15<sup>th </sup>2010, 1633 potential 2009 H1N1v cases have been screened at our center using the CDC detection and typing realtime RT-PCR assays. Virus isolation on MDCK cells was systematically performed in 1/10 positive cases. A subset of 51 H1N1v strains isolated in the period May-September 2009 was selected for NS1 gene sequencing. In addition, 15 H1N1 and 47 H3N2 virus isolates from three previous seasonal epidemics (2006-2009) were analyzed in parallel.</p></sec><sec><title>Results</title><p>A low variability in the NS1 amino acid (aa) sequence among H1N1v isolates was shown (aa identity 99.5%). A slightly higher NS1 variability was observed among H1N1 and H3N2 strains from previous epidemics (aa identity 98.6% and 98.9%, respectively). The H1N1v strains were closely related (aa identity 92.1%) to swine reference strain (A/swine/Oklahoma/042169/2008). In contrast, substantial divergence (aa identity 83.4%) with respect to human reference strain A/Brevig Mission/1/1918 and previous epidemic strains H1N1 and H3N2 (aa identity 78.9% and 77.6%, respectively) was shown. Specific sequence signatures of uncertain significance in the new virus variant were a C-terminus deletion and a T215P substitution.</p></sec><sec><title>Conclusions</title><p>The H1N1v NS1 gene was more conserved than that of previous epidemic strains. In addition, a closer genetic identity of H1N1v with the swine than the human reference strains was shown. Hot-spots were shown in the H1N1v NS1 aa sequence whose biologic relevance remains to be investigated.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name></author><author><name sortKey="Biron, Ca" uniqKey="Biron C">CA Biron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Newby, Cm" uniqKey="Newby C">CM Newby</name></author><author><name sortKey="Sabin, L" uniqKey="Sabin L">L Sabin</name></author><author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A Pekosz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fernandez Sesm, A" uniqKey="Fernandez Sesm A">A Fernandez-Sesm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hale, Gh" uniqKey="Hale G">GH Hale</name></author><author><name sortKey="Randall, Re" uniqKey="Randall R">RE Randall</name></author><author><name sortKey="Ortin, J" uniqKey="Ortin J">J Ortìn</name></author><author><name sortKey="Jackson, D" uniqKey="Jackson D">D Jackson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melen, K" uniqKey="Melen K">K Melen</name></author><author><name sortKey="Kinnunen, L" uniqKey="Kinnunen L">L Kinnunen</name></author><author><name sortKey="Fagerlund, R" uniqKey="Fagerlund R">R Fagerlund</name></author><author><name sortKey="Ikonen, N" uniqKey="Ikonen N">N Ikonen</name></author><author><name sortKey="Twu, Ky" uniqKey="Twu K">KY Twu</name></author><author><name sortKey="Krug, Rm" uniqKey="Krug R">RM Krug</name></author><author><name sortKey="Iulkunen, I" uniqKey="Iulkunen I">I Iulkunen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Obenauer, Jc" uniqKey="Obenauer J">JC Obenauer</name></author><author><name sortKey="Denson, J" uniqKey="Denson J">J Denson</name></author><author><name sortKey="Mehta, Pk" uniqKey="Mehta P">PK Mehta</name></author><author><name sortKey="Su, X" uniqKey="Su X">X Su</name></author><author><name sortKey="Mukatira, S" uniqKey="Mukatira S">S Mukatira</name></author><author><name sortKey="Finkelstein, Db" uniqKey="Finkelstein D">DB Finkelstein</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Fan, Y" uniqKey="Fan Y">Y Fan</name></author><author><name sortKey="Rakestraw, Km" uniqKey="Rakestraw K">KM Rakestraw</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name></author><author><name sortKey="Krauss, S" uniqKey="Krauss S">S Krauss</name></author><author><name sortKey="Zheng, J" uniqKey="Zheng J">J Zheng</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Naeve, Cv" uniqKey="Naeve C">CV Naeve</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author><author><name sortKey="Shaw, Ml" uniqKey="Shaw M">ML Shaw</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, Gjd" uniqKey="Smith G">GJD Smith</name></author><author><name sortKey="Vijaykrishna, D" uniqKey="Vijaykrishna D">D Vijaykrishna</name></author><author><name sortKey="Bahl, J" uniqKey="Bahl J">J Bahl</name></author><author><name sortKey="Lycett, Sj" uniqKey="Lycett S">SJ Lycett</name></author><author><name sortKey="Worobey, M" uniqKey="Worobey M">M Worobey</name></author><author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name></author><author><name sortKey="Ma, Sk" uniqKey="Ma S">SK Ma</name></author><author><name sortKey="Cheung, Cm" uniqKey="Cheung C">CM Cheung</name></author><author><name sortKey="Raghwani, J" uniqKey="Raghwani J">J Raghwani</name></author><author><name sortKey="Bhatt, S" uniqKey="Bhatt S">S Bhatt</name></author><author><name sortKey="Peiris, Jsm" uniqKey="Peiris J">JSM Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Geiss, Gk" uniqKey="Geiss G">GK Geiss</name></author><author><name sortKey="Salvatore, M" uniqKey="Salvatore M">M Salvatore</name></author><author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name></author><author><name sortKey="Carter, Vs" uniqKey="Carter V">VS Carter</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Basler, Cf" uniqKey="Basler C">CF Basler</name></author><author><name sortKey="Tauben Berger, Jk" uniqKey="Tauben Berger J">JK Tauben-berger</name></author><author><name sortKey="Bumgarner, Re" uniqKey="Bumgarner R">RE Bumgarner</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author><author><name sortKey="Katze, Mg" uniqKey="Katze M">MG Katze</name></author><author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seo, Sh" uniqKey="Seo S">SH Seo</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seo, Sh" uniqKey="Seo S">SH Seo</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Treanor, Jj" uniqKey="Treanor J">JJ Treanor</name></author><author><name sortKey="Snyder, Mh" uniqKey="Snyder M">MH Snyder</name></author><author><name sortKey="London, Wt" uniqKey="London W">WT London</name></author><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, S" uniqKey="Ludwig S">S Ludwig</name></author><author><name sortKey="Shultz, U" uniqKey="Shultz U">U Shultz</name></author><author><name sortKey="Mandler, J" uniqKey="Mandler J">J Mandler</name></author><author><name sortKey="Fitch, Wm" uniqKey="Fitch W">WM Fitch</name></author><author><name sortKey="Scholtissek, C" uniqKey="Scholtissek C">C Scholtissek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zohari, S" uniqKey="Zohari S">S Zohari</name></author><author><name sortKey="Gyarmati, P" uniqKey="Gyarmati P">P Gyarmati</name></author><author><name sortKey="Thoren, P" uniqKey="Thoren P">P Thoren</name></author><author><name sortKey="Czifra, G" uniqKey="Czifra G">G Czifra</name></author><author><name sortKey="Brojer, C" uniqKey="Brojer C">C Brojer</name></author><author><name sortKey="Belak, S" uniqKey="Belak S">S Belak</name></author><author><name sortKey="Berg, M" uniqKey="Berg M">M Berg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rovida, F" uniqKey="Rovida F">F Rovida</name></author><author><name sortKey="Percivalle, E" uniqKey="Percivalle E">E Percivalle</name></author><author><name sortKey="Zavattoni, M" uniqKey="Zavattoni M">M Zavattoni</name></author><author><name sortKey="Torsellini, M" uniqKey="Torsellini M">M Torsellini</name></author><author><name sortKey="Sarasini, A" uniqKey="Sarasini A">A Sarasini</name></author><author><name sortKey="Campanini, G" uniqKey="Campanini G">G Campanini</name></author><author><name sortKey="Paolucci, S" uniqKey="Paolucci S">S Paolucci</name></author><author><name sortKey="Baldanti, F" uniqKey="Baldanti F">F Baldanti</name></author><author><name sortKey="Revello, Mg" uniqKey="Revello M">MG Revello</name></author><author><name sortKey="Gerna, G" uniqKey="Gerna G">G Gerna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sarasini, A" uniqKey="Sarasini A">A Sarasini</name></author><author><name sortKey="Percivalle, E" uniqKey="Percivalle E">E Percivalle</name></author><author><name sortKey="Rovida, F" uniqKey="Rovida F">F Rovida</name></author><author><name sortKey="Campanini, G" uniqKey="Campanini G">G Campanini</name></author><author><name sortKey="Genini, E" uniqKey="Genini E">E Genini</name></author><author><name sortKey="Torsellini, M" uniqKey="Torsellini M">M Torsellini</name></author><author><name sortKey="Paolucci, S" uniqKey="Paolucci S">S Paolucci</name></author><author><name sortKey="Baldanti, F" uniqKey="Baldanti F">F Baldanti</name></author><author><name sortKey="Marchi, A" uniqKey="Marchi A">A Marchi</name></author><author><name sortKey="Revello, Mg" uniqKey="Revello M">MG Revello</name></author><author><name sortKey="Gerna, G" uniqKey="Gerna G">G Gerna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Percivalle, E" uniqKey="Percivalle E">E Percivalle</name></author><author><name sortKey="Rovida, F" uniqKey="Rovida F">F Rovida</name></author><author><name sortKey="Piralla, A" uniqKey="Piralla A">A Piralla</name></author><author><name sortKey="Rognoni, V" uniqKey="Rognoni V">V Rognoni</name></author><author><name sortKey="Zavattoni, M" uniqKey="Zavattoni M">M Zavattoni</name></author><author><name sortKey="Baldanti, F" uniqKey="Baldanti F">F Baldanti</name></author><author><name sortKey="Marchi, A" uniqKey="Marchi A">A Marchi</name></author><author><name sortKey="Gerna, G" uniqKey="Gerna G">G Gerna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author><author><name sortKey="Dudley, J" uniqKey="Dudley J">J Dudley</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name></author><author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name></author><author><name sortKey="Nelson, Mi" uniqKey="Nelson M">MI Nelson</name></author><author><name sortKey="Viboud, C" uniqKey="Viboud C">C Viboud</name></author><author><name sortKey="Tauenberger, Jk" uniqKey="Tauenberger J">JK Tauenberger</name></author><author><name sortKey="Holmes, Ec" uniqKey="Holmes E">EC Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenbaum, Ja" uniqKey="Greenbaum J">JA Greenbaum</name></author><author><name sortKey="Kotturi, Mf" uniqKey="Kotturi M">MF Kotturi</name></author><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y Kim</name></author><author><name sortKey="Oseroff, C" uniqKey="Oseroff C">C Oseroff</name></author><author><name sortKey="Vaughan, K" uniqKey="Vaughan K">K Vaughan</name></author><author><name sortKey="Salimi, N" uniqKey="Salimi N">N Salimi</name></author><author><name sortKey="Vita, R" uniqKey="Vita R">R Vita</name></author><author><name sortKey="Ponomarenco, J" uniqKey="Ponomarenco J">J Ponomarenco</name></author><author><name sortKey="Scheuermann, Rh" uniqKey="Scheuermann R">RH Scheuermann</name></author><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name></author><author><name sortKey="Peters, B" uniqKey="Peters B">B Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suarez, Dl" uniqKey="Suarez D">DL Suarez</name></author><author><name sortKey="Perdue, Ml" uniqKey="Perdue M">ML Perdue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackson, D" uniqKey="Jackson D">D Jackson</name></author><author><name sortKey="Hossain, Mj" uniqKey="Hossain M">MJ Hossain</name></author><author><name sortKey="Hickman, D" uniqKey="Hickman D">D Hickman</name></author><author><name sortKey="Perez, Dr" uniqKey="Perez D">DR Perez</name></author><author><name sortKey="Lamb, Ra" uniqKey="Lamb R">RA Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dundon, Wg" uniqKey="Dundon W">WG Dundon</name></author><author><name sortKey="Milani, A" uniqKey="Milani A">A Milani</name></author><author><name sortKey="Cattoli, G" uniqKey="Cattoli G">G Cattoli</name></author><author><name sortKey="Capua, I" uniqKey="Capua I">I Capua</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Min, Jy" uniqKey="Min J">JY Min</name></author><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author><author><name sortKey="Sen, Gc" uniqKey="Sen G">GC Sen</name></author><author><name sortKey="Krug, Rm" uniqKey="Krug R">RM Krug</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virol J</journal-id><journal-title-group><journal-title>Virology Journal</journal-title></journal-title-group><issn pub-type="epub">1743-422X</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20809948</article-id><article-id pub-id-type="pmc">2936903</article-id><article-id pub-id-type="publisher-id">1743-422X-7-209</article-id><article-id pub-id-type="doi">10.1186/1743-422X-7-209</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Campanini</surname><given-names>Giulia</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>g.campanini@smatteo.pv.it</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Piralla</surname><given-names>Antonio</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>antoniopiralla@yahoo.it</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Paolucci</surname><given-names>Stefania</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>s.paolucci@smatteo.pv.it</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Rovida</surname><given-names>Francesca</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>frarovi@yahoo.com</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Percivalle</surname><given-names>Elena</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>e.percivalle@smatteo.pv.it</email></contrib><contrib contrib-type="author" id="A6"><name><surname>Maga</surname><given-names>Giovanni</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>maga@igm.cnr.it</email></contrib><contrib contrib-type="author" corresp="yes" id="A7"><name><surname>Baldanti</surname><given-names>Fausto</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>f.baldanti@smatteo.pv.it</email></contrib></contrib-group><aff id="I1"><label>1</label>Molecular Virology Unit, Virology and Microbiology Dept, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy</aff><aff id="I2"><label>2</label>National Research Center IGBE CNR, 27100 Pavia, Italy</aff><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>1</day><month>9</month><year>2010</year></pub-date><volume>7</volume><fpage>209</fpage><lpage>209</lpage><history><date date-type="received"><day>26</day><month>7</month><year>2010</year></date><date date-type="accepted"><day>1</day><month>9</month><year>2010</year></date></history><permissions><copyright-statement>Copyright ©2010 Campanini et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2010</copyright-year><copyright-holder>Campanini et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.virologyj.com/content/7/1/209"></self-uri><abstract><sec><title>Background</title><p>The Influenza A pandemic sustained by a new H1N1 variant (H1N1v) started in Mexico and the USA at the end of April 2009 spreading worldwide in a few weeks. In this study we investigate the variability of the NS1 gene of the pandemic H1N1v strain with respect to previous seasonal strains circulating in humans and the potential selection of virus variants through isolation in cell culture.</p></sec><sec><title>Methods</title><p>During the period April 27<sup>th </sup>2009-Jan 15<sup>th </sup>2010, 1633 potential 2009 H1N1v cases have been screened at our center using the CDC detection and typing realtime RT-PCR assays. Virus isolation on MDCK cells was systematically performed in 1/10 positive cases. A subset of 51 H1N1v strains isolated in the period May-September 2009 was selected for NS1 gene sequencing. In addition, 15 H1N1 and 47 H3N2 virus isolates from three previous seasonal epidemics (2006-2009) were analyzed in parallel.</p></sec><sec><title>Results</title><p>A low variability in the NS1 amino acid (aa) sequence among H1N1v isolates was shown (aa identity 99.5%). A slightly higher NS1 variability was observed among H1N1 and H3N2 strains from previous epidemics (aa identity 98.6% and 98.9%, respectively). The H1N1v strains were closely related (aa identity 92.1%) to swine reference strain (A/swine/Oklahoma/042169/2008). In contrast, substantial divergence (aa identity 83.4%) with respect to human reference strain A/Brevig Mission/1/1918 and previous epidemic strains H1N1 and H3N2 (aa identity 78.9% and 77.6%, respectively) was shown. Specific sequence signatures of uncertain significance in the new virus variant were a C-terminus deletion and a T215P substitution.</p></sec><sec><title>Conclusions</title><p>The H1N1v NS1 gene was more conserved than that of previous epidemic strains. In addition, a closer genetic identity of H1N1v with the swine than the human reference strains was shown. Hot-spots were shown in the H1N1v NS1 aa sequence whose biologic relevance remains to be investigated.</p></sec></abstract></article-meta></front></pmc><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Campanini, Giulia" sort="Campanini, Giulia" uniqKey="Campanini G" first="Giulia" last="Campanini">Giulia Campanini</name></noRegion><name sortKey="Baldanti, Fausto" sort="Baldanti, Fausto" uniqKey="Baldanti F" first="Fausto" last="Baldanti">Fausto Baldanti</name><name sortKey="Maga, Giovanni" sort="Maga, Giovanni" uniqKey="Maga G" first="Giovanni" last="Maga">Giovanni Maga</name><name sortKey="Paolucci, Stefania" sort="Paolucci, Stefania" uniqKey="Paolucci S" first="Stefania" last="Paolucci">Stefania Paolucci</name><name sortKey="Percivalle, Elena" sort="Percivalle, Elena" uniqKey="Percivalle E" first="Elena" last="Percivalle">Elena Percivalle</name><name sortKey="Piralla, Antonio" sort="Piralla, Antonio" uniqKey="Piralla A" first="Antonio" last="Piralla">Antonio Piralla</name><name sortKey="Rovida, Francesca" sort="Rovida, Francesca" uniqKey="Rovida F" first="Francesca" last="Rovida">Francesca Rovida</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000562 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000562 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= PMC:2936903
|texte= Genetic divergence of influenza A NS1 gene in pandemic 2009 H1N1 isolates with respect to H1N1 and H3N2 isolates from previous seasonal epidemics
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:20809948" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |