A new RT-PCR assay specific for influenza A(H2), multiplexed with an assay specific for HPAI A(H5N1).
Identifieur interne : 000559 ( Ncbi/Merge ); précédent : 000558; suivant : 000560A new RT-PCR assay specific for influenza A(H2), multiplexed with an assay specific for HPAI A(H5N1).
Auteurs : C. Yea [Canada] ; M. Petric ; J. Pasick ; R. TellierSource :
- Molecular and cellular probes [ 1096-1194 ] ; 2010.
Descripteurs français
- KwdFr :
- Alignement de séquences, Amorces ADN (métabolisme), Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Dosage biologique (), Grippe chez les oiseaux (virologie), Grippe chez les oiseaux (épidémiologie), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains, Oiseaux (virologie), Pandémies, RT-PCR (), Reproductibilité des résultats, Sensibilité et spécificité, Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (isolement et purification), Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (isolement et purification), Spécificité d'espèce, Sus scrofa (virologie), Séquence nucléotidique.
- MESH :
- génétique : Sous-type H2N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- isolement et purification : Sous-type H2N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- métabolisme : Amorces ADN.
- virologie : Grippe chez les oiseaux, Grippe humaine, Oiseaux, Sus scrofa.
- épidémiologie : Grippe chez les oiseaux, Grippe humaine.
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Dosage biologique, Humains, Pandémies, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Spécificité d'espèce, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Biological Assay (methods), Birds (virology), DNA Primers (metabolism), Humans, Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (isolation & purification), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (isolation & purification), Influenza in Birds (epidemiology), Influenza in Birds (virology), Influenza, Human (epidemiology), Influenza, Human (virology), Molecular Sequence Data, Pandemics, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Sequence Alignment, Sequence Analysis, DNA, Species Specificity, Sus scrofa (virology).
- MESH :
- chemical , metabolism : DNA Primers.
- epidemiology : Influenza in Birds, Influenza, Human.
- genetics : Influenza A Virus, H2N2 Subtype, Influenza A Virus, H5N1 Subtype.
- isolation & purification : Influenza A Virus, H2N2 Subtype, Influenza A Virus, H5N1 Subtype.
- methods : Biological Assay, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Birds, Influenza in Birds, Influenza, Human, Sus scrofa.
- Animals, Base Sequence, Humans, Molecular Sequence Data, Pandemics, Reproducibility of Results, Sensitivity and Specificity, Sequence Alignment, Sequence Analysis, DNA, Species Specificity.
Abstract
Worldwide efforts are ongoing to improve influenza pandemic preparedness, including from the perspective of the clinical virology laboratory. In particular, much work has been devoted to the development of diagnostic assays targeted at the Highly Pathogenic Avian Influenza (HPAI) A(H5N1); much less efforts have been devoted to the A(H2) subtype. Yet, A(H2) subtype has a proven capacity to cause pandemics and is among the subtypes prioritized for surveillance and control. Although the human A(H2N2) virus that caused the pandemic of 1957 no longer circulates, many related avian A(H2) viruses circulate in avian population and could conceivably adapt to infect humans and cause a new pandemic. In this study the design and development of an RT-PCR assay specific for A(H2) subtype is presented. It is shown that the assay is highly sensitive and specific, able to detect human and avian A(H2) viruses, and can be incorporated into a multiplex assay with another previously described assay for HPAI A(H5N1).
DOI: 10.1016/j.mcp.2010.08.001
PubMed: 20727404
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pubmed:20727404Le document en format XML
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It is shown that the assay is highly sensitive and specific, able to detect human and avian A(H2) viruses, and can be incorporated into a multiplex assay with another previously described assay for HPAI A(H5N1).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20727404</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1194</ISSN><JournalIssue CitedMedium="Internet"><Volume>24</Volume><Issue>6</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular and cellular probes</Title><ISOAbbreviation>Mol. Cell. Probes</ISOAbbreviation></Journal><ArticleTitle>A new RT-PCR assay specific for influenza A(H2), multiplexed with an assay specific for HPAI A(H5N1).</ArticleTitle><Pagination><MedlinePgn>364-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mcp.2010.08.001</ELocationID><Abstract><AbstractText>Worldwide efforts are ongoing to improve influenza pandemic preparedness, including from the perspective of the clinical virology laboratory. In particular, much work has been devoted to the development of diagnostic assays targeted at the Highly Pathogenic Avian Influenza (HPAI) A(H5N1); much less efforts have been devoted to the A(H2) subtype. Yet, A(H2) subtype has a proven capacity to cause pandemics and is among the subtypes prioritized for surveillance and control. Although the human A(H2N2) virus that caused the pandemic of 1957 no longer circulates, many related avian A(H2) viruses circulate in avian population and could conceivably adapt to infect humans and cause a new pandemic. In this study the design and development of an RT-PCR assay specific for A(H2) subtype is presented. It is shown that the assay is highly sensitive and specific, able to detect human and avian A(H2) viruses, and can be incorporated into a multiplex assay with another previously described assay for HPAI A(H5N1).</AbstractText><CopyrightInformation>Copyright © 2010 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yea</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Genetics and Genome Biology Program, Research Institute, Hospital for Sick Children, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Petric</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Pasick</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Tellier</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053121" MajorTopicYN="N">Influenza A Virus, H2N2 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName><QualifierName 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Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D034421" MajorTopicYN="N">Sus scrofa</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>12</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>08</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>2</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20727404</ArticleId><ArticleId IdType="pii">S0890-8508(10)00050-2</ArticleId><ArticleId IdType="doi">10.1016/j.mcp.2010.08.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Pasick, J" sort="Pasick, J" uniqKey="Pasick J" first="J" last="Pasick">J. Pasick</name><name sortKey="Petric, M" sort="Petric, M" uniqKey="Petric M" first="M" last="Petric">M. Petric</name><name sortKey="Tellier, R" sort="Tellier, R" uniqKey="Tellier R" first="R" last="Tellier">R. Tellier</name></noCountry><country name="Canada"><noRegion><name sortKey="Yea, C" sort="Yea, C" uniqKey="Yea C" first="C" last="Yea">C. Yea</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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