[The NS gene--a possible determinant of apathogenicity of a cold-adapted donor of attenuation A /Leningrad/134/47/57 and its reassortants].
Identifieur interne : 001294 ( Ncbi/Curation ); précédent : 001293; suivant : 001295[The NS gene--a possible determinant of apathogenicity of a cold-adapted donor of attenuation A /Leningrad/134/47/57 and its reassortants].
Auteurs : A Iu Egorov ; L M Garmashova ; I V Lukashok ; G N Nevedomskaia ; G I Aleksandrova ; A I KlimovSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Adaptation physiologique, Animaux, Basse température, Carnivora, Cellules cultivées, Chiens, Phénotype, Poulets, Protéines virales non structurales (biosynthèse), Protéines virales non structurales (génétique), Souris, Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins atténués (génétique), Vaccins atténués (immunologie), Virulence (génétique), Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Virus de la grippe A (pathogénicité).
- MESH :
- biosynthèse : Protéines virales non structurales.
- génétique : Protéines virales non structurales, Vaccins antiviraux, Vaccins atténués, Virulence, Virus de la grippe A.
- immunologie : Vaccins antiviraux, Vaccins atténués, Virus de la grippe A.
- pathogénicité : Virus de la grippe A.
- Adaptation physiologique, Animaux, Basse température, Carnivora, Cellules cultivées, Chiens, Phénotype, Poulets, Souris.
English descriptors
- KwdEn :
- Adaptation, Physiological, Animals, Carnivora, Cells, Cultured, Chickens, Cold Temperature, Dogs, Influenza A virus (genetics), Influenza A virus (immunology), Influenza A virus (pathogenicity), Mice, Phenotype, Vaccines, Attenuated (genetics), Vaccines, Attenuated (immunology), Viral Nonstructural Proteins (biosynthesis), Viral Nonstructural Proteins (genetics), Viral Vaccines (genetics), Viral Vaccines (immunology), Virulence (genetics).
- MESH :
- chemical , biosynthesis : Viral Nonstructural Proteins.
- chemical , genetics : Vaccines, Attenuated, Viral Nonstructural Proteins, Viral Vaccines.
- genetics : Influenza A virus, Virulence.
- immunology : Influenza A virus, Vaccines, Attenuated, Viral Vaccines.
- pathogenicity : Influenza A virus.
- Adaptation, Physiological, Animals, Carnivora, Cells, Cultured, Chickens, Cold Temperature, Dogs, Mice, Phenotype.
Abstract
Phenotypical properties of single-gene reassortants of attenuated cold-adapted strain A/Leningrad/135/47/57 (H2N2) and strain A/PR8/34 virulent for laboratory animals were studied. Only the group of reassortants inheriting NS gene from cold-adapted virus was fully attenuated for various animals species, similarly as reassortants with 6/2 genomic formula containing all the 6 internal protein genes from strain A/Leningrad/134/47/57. Reassortant 25A-1 single-gene for NS was temperature-sensitive (ts) on mammalian cells but formed plaques at 40 degrees C on chicken kidney cells. Reassortants with genomic formula 6/2 were temperature-sensitive in all types of cells used. Reassortant 25A-1 could synthesize normal amounts of polypeptides in MDCK cells at 39 degrees C, whereas protein synthesis of reassortants with 6/2 genomic formula was noticeably reduced at this temperature. Hence, a similar level of attenuation of both reassortant groups appears to be due to various molecular mechanisms. Possible role of NS2 gene mutation in attenuation of strain A/Leningrad/134/47/57 and its reassortants is discussed.
PubMed: 7716901
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pubmed:7716901Le document en format XML
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Physiological</term><term>Animals</term><term>Carnivora</term><term>Cells, Cultured</term><term>Chickens</term><term>Cold Temperature</term><term>Dogs</term><term>Mice</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Carnivora</term><term>Cellules cultivées</term><term>Chiens</term><term>Phénotype</term><term>Poulets</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phenotypical properties of single-gene reassortants of attenuated cold-adapted strain A/Leningrad/135/47/57 (H2N2) and strain A/PR8/34 virulent for laboratory animals were studied. Only the group of reassortants inheriting NS gene from cold-adapted virus was fully attenuated for various animals species, similarly as reassortants with 6/2 genomic formula containing all the 6 internal protein genes from strain A/Leningrad/134/47/57. Reassortant 25A-1 single-gene for NS was temperature-sensitive (ts) on mammalian cells but formed plaques at 40 degrees C on chicken kidney cells. Reassortants with genomic formula 6/2 were temperature-sensitive in all types of cells used. Reassortant 25A-1 could synthesize normal amounts of polypeptides in MDCK cells at 39 degrees C, whereas protein synthesis of reassortants with 6/2 genomic formula was noticeably reduced at this temperature. Hence, a similar level of attenuation of both reassortant groups appears to be due to various molecular mechanisms. Possible role of NS2 gene mutation in attenuation of strain A/Leningrad/134/47/57 and its reassortants is discussed.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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