[Analysis of the potentials of molecular hybridization of nucleic acids as a method of the laboratory diagnosis of influenza].
Identifieur interne : 001064 ( Ncbi/Checkpoint ); précédent : 001063; suivant : 001065[Analysis of the potentials of molecular hybridization of nucleic acids as a method of the laboratory diagnosis of influenza].
Auteurs : A Z Pliusnin ; S A Rozhkova ; O V Nolandt ; E A Briantseva ; F S NoskovSource :
- Zhurnal mikrobiologii, epidemiologii, i immunobiologii [ 0372-9311 ] ; 1988.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ADN recombiné, ADN viral (génétique), ARN viral (génétique), Animaux, Embryon de poulet, Grippe humaine (diagnostic), Grippe humaine (microbiologie), Gènes viraux, Humains, Hybridation d'acides nucléiques, Plasmides, Techniques génétiques, Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification).
- MESH :
- diagnostic : Grippe humaine.
- génétique : ADN bactérien, ADN viral, ARN viral, Virus de la grippe A.
- isolement et purification : Virus de la grippe A.
- microbiologie : Grippe humaine.
- ADN recombiné, Animaux, Embryon de poulet, Gènes viraux, Humains, Hybridation d'acides nucléiques, Plasmides, Techniques génétiques.
English descriptors
- KwdEn :
- Animals, Chick Embryo, DNA, Bacterial (genetics), DNA, Recombinant, DNA, Viral (genetics), Genes, Viral, Genetic Techniques, Humans, Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (microbiology), Nucleic Acid Hybridization, Plasmids, RNA, Viral (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial, DNA, Viral, RNA, Viral.
- diagnosis : Influenza, Human.
- genetics : Influenza A virus.
- isolation & purification : Influenza A virus.
- microbiology : Influenza, Human.
- Animals, Chick Embryo, DNA, Recombinant, Genes, Viral, Genetic Techniques, Humans, Nucleic Acid Hybridization, Plasmids.
Abstract
The possibilities of using the DNA copies of different genes of influenza A virus for the detection of virus-specific RNa by molecular dot hybridization have been studied. High specificity and sensitivity of the RNA determination techniques have been demonstrated, as well as the efficacy of using DNA probes with the sequences of conservative genes (polymerase, nucleoprotein and matrix genes) for the detection of influenza A virus subtypes H1N1, H2N2, H3N2 and probes with the copies of the corresponding hemagglutinin genes for the differential determination of subtypes H3N2 and H1N1. The complex analysis of nasopharyngeal washings has confirmed the efficacy of the dot hybridization method for epidemiological investigations, particularly for deciphering influenza outbreaks, especially those of mixed etiology.
PubMed: 3291499
Affiliations:
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pubmed:3291499Le document en format XML
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<series><title level="j">Zhurnal mikrobiologii, epidemiologii, i immunobiologii</title>
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<term>Chick Embryo</term>
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<term>DNA, Recombinant</term>
<term>DNA, Viral (genetics)</term>
<term>Genes, Viral</term>
<term>Genetic Techniques</term>
<term>Humans</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (microbiology)</term>
<term>Nucleic Acid Hybridization</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term>
<term>ADN recombiné</term>
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<term>ARN viral (génétique)</term>
<term>Animaux</term>
<term>Embryon de poulet</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (microbiologie)</term>
<term>Gènes viraux</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Plasmides</term>
<term>Techniques génétiques</term>
<term>Virus de la grippe A (génétique)</term>
<term>Virus de la grippe A (isolement et purification)</term>
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<term>RNA, Viral</term>
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<term>Chick Embryo</term>
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<term>Genes, Viral</term>
<term>Genetic Techniques</term>
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<term>Nucleic Acid Hybridization</term>
<term>Plasmids</term>
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<term>Embryon de poulet</term>
<term>Gènes viraux</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">The possibilities of using the DNA copies of different genes of influenza A virus for the detection of virus-specific RNa by molecular dot hybridization have been studied. High specificity and sensitivity of the RNA determination techniques have been demonstrated, as well as the efficacy of using DNA probes with the sequences of conservative genes (polymerase, nucleoprotein and matrix genes) for the detection of influenza A virus subtypes H1N1, H2N2, H3N2 and probes with the copies of the corresponding hemagglutinin genes for the differential determination of subtypes H3N2 and H1N1. The complex analysis of nasopharyngeal washings has confirmed the efficacy of the dot hybridization method for epidemiological investigations, particularly for deciphering influenza outbreaks, especially those of mixed etiology.</div>
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<name sortKey="Nolandt, O V" sort="Nolandt, O V" uniqKey="Nolandt O" first="O V" last="Nolandt">O V Nolandt</name>
<name sortKey="Noskov, F S" sort="Noskov, F S" uniqKey="Noskov F" first="F S" last="Noskov">F S Noskov</name>
<name sortKey="Pliusnin, A Z" sort="Pliusnin, A Z" uniqKey="Pliusnin A" first="A Z" last="Pliusnin">A Z Pliusnin</name>
<name sortKey="Rozhkova, S A" sort="Rozhkova, S A" uniqKey="Rozhkova S" first="S A" last="Rozhkova">S A Rozhkova</name>
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