Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs
Identifieur interne : 000D33 ( Ncbi/Checkpoint ); précédent : 000D32; suivant : 000D34Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs
Auteurs : Jinhwa Lee ; Jamie Henningson ; Jingjiao Ma ; Michael Duff ; Yuekun Lang ; Yonghai Li ; Yuhao Li ; Abdou Nagy ; Sunyoung Sunwoo ; Bhupinder Bawa ; Jianmei Yang ; Dingping Bai ; Juergen A. Richt ; Wenjun MaSource :
- The Journal of General Virology [ 0022-1317 ] ; 2017.
Abstract
Although several studies have exploited the effects of PB1-F2 in swine influenza viruses, its contribution to the pathogenicity of swine influenza viruses remains unclear. Herein, we investigated the effects of PB1-F2 on the pathogenicity of influenza virus using a virulent H1N1 A/swine/Kansas/77778/2007 (KS07) virus, which expresses a full-length PB1-F2, in mice and pigs. Using reverse genetics, we generated the wild-type KS07 (KS07_WT), a PB1-F2 knockout mutant (KS07_K/O) and its N66S variant (KS07_N66S). KS07_K/O showed similar pathogenicity in mice to the KS07_WT, whereas KS07_N66S displayed enhanced virulence when compared to the other two viruses. KS07_WT exhibited more efficient replication in lungs and nasal shedding in infected pigs than the other two viruses. Pigs infected with the KS07_WT had higher pulmonary levels of granulocyte-macrophage colony-stimulating factor, IFN-γ, IL-6 and IL-8 at 3 and 5 days post-infection, as well as lower levels of IL-2, IL-4 and IL-12 at 1 day post-infection compared to those infected with the KS07_K/O. These results indicate that PB1-F2 modulates KS07 H1N1 virus replication, pathogenicity and innate immune responses in pigs and the single substitution at position 66 (N/S) in the PB1-F2 plays a critical role in virulence in mice. Taken together, our results provide new insights into the effects of PB1-F2 on the virulence of influenza virus in swine and support PB1-F2 as a virulence factor of influenza A virus in a strain- and host-dependent manner.
Url:
DOI: 10.1099/jgv.0.000695
PubMed: 28008819
PubMed Central: 5797944
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000627
- to stream Pmc, to step Curation: 000627
- to stream Pmc, to step Checkpoint: 000245
- to stream Ncbi, to step Merge: 000D33
- to stream Ncbi, to step Curation: 000D33
Links to Exploration step
PMC:5797944Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs</title>
<author><name sortKey="Lee, Jinhwa" sort="Lee, Jinhwa" uniqKey="Lee J" first="Jinhwa" last="Lee">Jinhwa Lee</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Henningson, Jamie" sort="Henningson, Jamie" uniqKey="Henningson J" first="Jamie" last="Henningson">Jamie Henningson</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ma, Jingjiao" sort="Ma, Jingjiao" uniqKey="Ma J" first="Jingjiao" last="Ma">Jingjiao Ma</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duff, Michael" sort="Duff, Michael" uniqKey="Duff M" first="Michael" last="Duff">Michael Duff</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lang, Yuekun" sort="Lang, Yuekun" uniqKey="Lang Y" first="Yuekun" last="Lang">Yuekun Lang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yonghai" sort="Li, Yonghai" uniqKey="Li Y" first="Yonghai" last="Li">Yonghai Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yuhao" sort="Li, Yuhao" uniqKey="Li Y" first="Yuhao" last="Li">Yuhao Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nagy, Abdou" sort="Nagy, Abdou" uniqKey="Nagy A" first="Abdou" last="Nagy">Abdou Nagy</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sunwoo, Sunyoung" sort="Sunwoo, Sunyoung" uniqKey="Sunwoo S" first="Sunyoung" last="Sunwoo">Sunyoung Sunwoo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bawa, Bhupinder" sort="Bawa, Bhupinder" uniqKey="Bawa B" first="Bhupinder" last="Bawa">Bhupinder Bawa</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Jianmei" sort="Yang, Jianmei" uniqKey="Yang J" first="Jianmei" last="Yang">Jianmei Yang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bai, Dingping" sort="Bai, Dingping" uniqKey="Bai D" first="Dingping" last="Bai">Dingping Bai</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Richt, Juergen A" sort="Richt, Juergen A" uniqKey="Richt J" first="Juergen A." last="Richt">Juergen A. Richt</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ma, Wenjun" sort="Ma, Wenjun" uniqKey="Ma W" first="Wenjun" last="Ma">Wenjun Ma</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">28008819</idno>
<idno type="pmc">5797944</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797944</idno>
<idno type="RBID">PMC:5797944</idno>
<idno type="doi">10.1099/jgv.0.000695</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000627</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000627</idno>
<idno type="wicri:Area/Pmc/Curation">000627</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000627</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000245</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000245</idno>
<idno type="wicri:Area/Ncbi/Merge">000D33</idno>
<idno type="wicri:Area/Ncbi/Curation">000D33</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000D33</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs</title>
<author><name sortKey="Lee, Jinhwa" sort="Lee, Jinhwa" uniqKey="Lee J" first="Jinhwa" last="Lee">Jinhwa Lee</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Henningson, Jamie" sort="Henningson, Jamie" uniqKey="Henningson J" first="Jamie" last="Henningson">Jamie Henningson</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ma, Jingjiao" sort="Ma, Jingjiao" uniqKey="Ma J" first="Jingjiao" last="Ma">Jingjiao Ma</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duff, Michael" sort="Duff, Michael" uniqKey="Duff M" first="Michael" last="Duff">Michael Duff</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lang, Yuekun" sort="Lang, Yuekun" uniqKey="Lang Y" first="Yuekun" last="Lang">Yuekun Lang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yonghai" sort="Li, Yonghai" uniqKey="Li Y" first="Yonghai" last="Li">Yonghai Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yuhao" sort="Li, Yuhao" uniqKey="Li Y" first="Yuhao" last="Li">Yuhao Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nagy, Abdou" sort="Nagy, Abdou" uniqKey="Nagy A" first="Abdou" last="Nagy">Abdou Nagy</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sunwoo, Sunyoung" sort="Sunwoo, Sunyoung" uniqKey="Sunwoo S" first="Sunyoung" last="Sunwoo">Sunyoung Sunwoo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bawa, Bhupinder" sort="Bawa, Bhupinder" uniqKey="Bawa B" first="Bhupinder" last="Bawa">Bhupinder Bawa</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Jianmei" sort="Yang, Jianmei" uniqKey="Yang J" first="Jianmei" last="Yang">Jianmei Yang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bai, Dingping" sort="Bai, Dingping" uniqKey="Bai D" first="Dingping" last="Bai">Dingping Bai</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Richt, Juergen A" sort="Richt, Juergen A" uniqKey="Richt J" first="Juergen A." last="Richt">Juergen A. Richt</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ma, Wenjun" sort="Ma, Wenjun" uniqKey="Ma W" first="Wenjun" last="Ma">Wenjun Ma</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of General Virology</title>
<idno type="ISSN">0022-1317</idno>
<idno type="eISSN">1465-2099</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Although several studies have exploited the effects of PB1-F2 in swine influenza viruses, its contribution to the pathogenicity of swine influenza viruses remains unclear. Herein, we investigated the effects of PB1-F2 on the pathogenicity of influenza virus using a virulent H1N1 A/swine/Kansas/77778/2007 (KS07) virus, which expresses a full-length PB1-F2, in mice and pigs. Using reverse genetics, we generated the wild-type KS07 (KS07_WT), a PB1-F2 knockout mutant (KS07_K/O) and its N66S variant (KS07_N66S). KS07_K/O showed similar pathogenicity in mice to the KS07_WT, whereas KS07_N66S displayed enhanced virulence when compared to the other two viruses. KS07_WT exhibited more efficient replication in lungs and nasal shedding in infected pigs than the other two viruses. Pigs infected with the KS07_WT had higher pulmonary levels of granulocyte-macrophage colony-stimulating factor, IFN-γ, IL-6 and IL-8 at 3 and 5 days post-infection, as well as lower levels of IL-2, IL-4 and IL-12 at 1 day post-infection compared to those infected with the KS07_K/O. These results indicate that PB1-F2 modulates KS07 H1N1 virus replication, pathogenicity and innate immune responses in pigs and the single substitution at position 66 (N/S) in the PB1-F2 plays a critical role in virulence in mice. Taken together, our results provide new insights into the effects of PB1-F2 on the virulence of influenza virus in swine and support PB1-F2 as a virulence factor of influenza A virus in a strain- and host-dependent manner.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Bai, Dingping" sort="Bai, Dingping" uniqKey="Bai D" first="Dingping" last="Bai">Dingping Bai</name>
<name sortKey="Bawa, Bhupinder" sort="Bawa, Bhupinder" uniqKey="Bawa B" first="Bhupinder" last="Bawa">Bhupinder Bawa</name>
<name sortKey="Duff, Michael" sort="Duff, Michael" uniqKey="Duff M" first="Michael" last="Duff">Michael Duff</name>
<name sortKey="Henningson, Jamie" sort="Henningson, Jamie" uniqKey="Henningson J" first="Jamie" last="Henningson">Jamie Henningson</name>
<name sortKey="Lang, Yuekun" sort="Lang, Yuekun" uniqKey="Lang Y" first="Yuekun" last="Lang">Yuekun Lang</name>
<name sortKey="Lee, Jinhwa" sort="Lee, Jinhwa" uniqKey="Lee J" first="Jinhwa" last="Lee">Jinhwa Lee</name>
<name sortKey="Li, Yonghai" sort="Li, Yonghai" uniqKey="Li Y" first="Yonghai" last="Li">Yonghai Li</name>
<name sortKey="Li, Yuhao" sort="Li, Yuhao" uniqKey="Li Y" first="Yuhao" last="Li">Yuhao Li</name>
<name sortKey="Ma, Jingjiao" sort="Ma, Jingjiao" uniqKey="Ma J" first="Jingjiao" last="Ma">Jingjiao Ma</name>
<name sortKey="Ma, Wenjun" sort="Ma, Wenjun" uniqKey="Ma W" first="Wenjun" last="Ma">Wenjun Ma</name>
<name sortKey="Nagy, Abdou" sort="Nagy, Abdou" uniqKey="Nagy A" first="Abdou" last="Nagy">Abdou Nagy</name>
<name sortKey="Richt, Juergen A" sort="Richt, Juergen A" uniqKey="Richt J" first="Juergen A." last="Richt">Juergen A. Richt</name>
<name sortKey="Sunwoo, Sunyoung" sort="Sunwoo, Sunyoung" uniqKey="Sunwoo S" first="Sunyoung" last="Sunwoo">Sunyoung Sunwoo</name>
<name sortKey="Yang, Jianmei" sort="Yang, Jianmei" uniqKey="Yang J" first="Jianmei" last="Yang">Jianmei Yang</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D33 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000D33 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= PMC:5797944 |texte= Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:28008819" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |