Neutralization and Binding Profile of Monoclonal Antibodies Generated Against Influenza A H1N1 Viruses.
Identifieur interne : 000C98 ( Ncbi/Checkpoint ); précédent : 000C97; suivant : 000C99Neutralization and Binding Profile of Monoclonal Antibodies Generated Against Influenza A H1N1 Viruses.
Auteurs : Nachiket Shembekar [Inde] ; Vamsee V Aditya Mallajosyula [Inde] ; Ankita Malik [Inde] ; Ashok Saini [Inde] ; Raghavan Varadarajan [Inde] ; Satish Kumar Gupta [Inde]Source :
- Monoclonal antibodies in immunodiagnosis and immunotherapy [ 2167-9436 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Anticorps neutralisants (immunologie), Glycoprotéine hémagglutinine du virus influenza (génétique), Glycoprotéine hémagglutinine du virus influenza (immunologie), Grippe humaine (), Grippe humaine (immunologie), Grippe humaine (virologie), Humains, Souris, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H1N1 du virus de la grippe A (pathogénicité), Tests d'inhibition de l'hémagglutination, Vaccins antigrippaux (immunologie).
- MESH :
- génétique : Glycoprotéine hémagglutinine du virus influenza.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Anticorps neutralisants, Glycoprotéine hémagglutinine du virus influenza, Grippe humaine, Sous-type H1N1 du virus de la grippe A, Vaccins antigrippaux.
- pathogénicité : Sous-type H1N1 du virus de la grippe A.
- virologie : Grippe humaine.
- Animaux, Grippe humaine, Humains, Souris, Tests d'inhibition de l'hémagglutination.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H1N1 Subtype (pathogenicity), Influenza Vaccines (immunology), Influenza, Human (immunology), Influenza, Human (prevention & control), Influenza, Human (virology), Mice.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza, Human.
- pathogenicity : Influenza A Virus, H1N1 Subtype.
- prevention & control : Influenza, Human.
- virology : Influenza, Human.
- Animals, Hemagglutination Inhibition Tests, Humans, Mice.
Abstract
Monoclonal antibodies (MAbs) provide scope for the development of better therapeutics and diagnostic tools. Herein, we describe the binding and neutralization profile(s) for a panel of murine MAbs generated against influenza A H1N1 viruses elicited by immunization with pandemic H1 recombinant hemagglutinin (rHA)/whole virus or seasonal H1 rHA. Neutralizing MAbs, MA-2070 and MA-M, were obtained after pandemic A/California/07/2009 (H1N1) virus/rHA immunization(s). Both MAbs reacted specifically with rHA from A/California/07/2009 and A/England/195/2009 in ELISA. MA-2070 bound rHA of A/California/07/2009 with high affinity (KD = 51.36 ± 9.20 nM) and exhibited potent in vitro neutralization (IC50 = 2.50 μg/mL). MA-2070 bound within the stem domain of HA. MA-M exhibited both hemagglutination inhibition (HI, 1.50 μg/mL) and in vitro neutralization (IC50 = 0.66 μg/mL) activity against the pandemic A/California/07/2009 virus and showed higher binding affinity (KD = 9.80 ± 0.67 nM) than MA-2070. MAb, MA-H generated against the seasonal A/Solomon Islands/03/2006 (H1N1) rHA binds within the head domain and bound the seasonal H1N1 (A/Solomon Islands/03/2006 and A/New Caledonia/20/1990) rHAs with high affinity (KD; 0.72-8.23 nM). MA-H showed high HI (2.50 μg/mL) and in vitro neutralization (IC50 = 2.61 μg/mL) activity against the A/Solomon Islands/03/2006 virus. All 3 MAbs failed to react in ELISA with rHA from various strains of H2N2, H3N2, H5N1, H7N9, and influenza virus B, suggesting their specificity for either pandemic or seasonal H1N1 influenza virus. The MAbs reported here may be useful in developing diagnostic assays.
DOI: 10.1089/mab.2016.0015
PubMed: 27463230
Affiliations:
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<term>Antibodies, Viral (immunology)</term>
<term>Hemagglutination Inhibition Tests</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (pathogenicity)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Influenza, Human (virology)</term>
<term>Mice</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (immunologie)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Souris</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H1N1 du virus de la grippe A (pathogénicité)</term>
<term>Tests d'inhibition de l'hémagglutination</term>
<term>Vaccins antigrippaux (immunologie)</term>
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<term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Influenza Vaccines</term>
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<term>Anticorps monoclonaux</term>
<term>Anticorps neutralisants</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Grippe humaine</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
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<term>Influenza, Human</term>
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</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term>
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<front><div type="abstract" xml:lang="en">Monoclonal antibodies (MAbs) provide scope for the development of better therapeutics and diagnostic tools. Herein, we describe the binding and neutralization profile(s) for a panel of murine MAbs generated against influenza A H1N1 viruses elicited by immunization with pandemic H1 recombinant hemagglutinin (rHA)/whole virus or seasonal H1 rHA. Neutralizing MAbs, MA-2070 and MA-M, were obtained after pandemic A/California/07/2009 (H1N1) virus/rHA immunization(s). Both MAbs reacted specifically with rHA from A/California/07/2009 and A/England/195/2009 in ELISA. MA-2070 bound rHA of A/California/07/2009 with high affinity (KD = 51.36 ± 9.20 nM) and exhibited potent in vitro neutralization (IC50 = 2.50 μg/mL). MA-2070 bound within the stem domain of HA. MA-M exhibited both hemagglutination inhibition (HI, 1.50 μg/mL) and in vitro neutralization (IC50 = 0.66 μg/mL) activity against the pandemic A/California/07/2009 virus and showed higher binding affinity (KD = 9.80 ± 0.67 nM) than MA-2070. MAb, MA-H generated against the seasonal A/Solomon Islands/03/2006 (H1N1) rHA binds within the head domain and bound the seasonal H1N1 (A/Solomon Islands/03/2006 and A/New Caledonia/20/1990) rHAs with high affinity (KD; 0.72-8.23 nM). MA-H showed high HI (2.50 μg/mL) and in vitro neutralization (IC50 = 2.61 μg/mL) activity against the A/Solomon Islands/03/2006 virus. All 3 MAbs failed to react in ELISA with rHA from various strains of H2N2, H3N2, H5N1, H7N9, and influenza virus B, suggesting their specificity for either pandemic or seasonal H1N1 influenza virus. The MAbs reported here may be useful in developing diagnostic assays. </div>
</front>
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<tree><country name="Inde"><noRegion><name sortKey="Shembekar, Nachiket" sort="Shembekar, Nachiket" uniqKey="Shembekar N" first="Nachiket" last="Shembekar">Nachiket Shembekar</name>
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<name sortKey="Gupta, Satish Kumar" sort="Gupta, Satish Kumar" uniqKey="Gupta S" first="Satish Kumar" last="Gupta">Satish Kumar Gupta</name>
<name sortKey="Malik, Ankita" sort="Malik, Ankita" uniqKey="Malik A" first="Ankita" last="Malik">Ankita Malik</name>
<name sortKey="Mallajosyula, Vamsee V Aditya" sort="Mallajosyula, Vamsee V Aditya" uniqKey="Mallajosyula V" first="Vamsee V Aditya" last="Mallajosyula">Vamsee V Aditya Mallajosyula</name>
<name sortKey="Saini, Ashok" sort="Saini, Ashok" uniqKey="Saini A" first="Ashok" last="Saini">Ashok Saini</name>
<name sortKey="Varadarajan, Raghavan" sort="Varadarajan, Raghavan" uniqKey="Varadarajan R" first="Raghavan" last="Varadarajan">Raghavan Varadarajan</name>
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