Reliability of Pseudotyped Influenza Viral Particles in Neutralizing Antibody Detection
Identifieur interne : 000B05 ( Ncbi/Checkpoint ); précédent : 000B04; suivant : 000B06Reliability of Pseudotyped Influenza Viral Particles in Neutralizing Antibody Detection
Auteurs : Jinghui Yang [République populaire de Chine] ; Weidong Li [République populaire de Chine] ; Yunfeng Long [République populaire de Chine] ; Shaohui Song [République populaire de Chine] ; Jing Liu [République populaire de Chine] ; Xinwen Zhang [République populaire de Chine] ; Xiaoguang Wang [République populaire de Chine] ; Shude Jiang [République populaire de Chine] ; Guoyang Liao [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
Current influenza control strategies require an active surveillance system. Pseudotyped viral particles (pp) together with the evaluation of pre-existing immunity in a population might satisfy this requirement. However, the reliability of using pp in neutralizing antibody (nAb) detection are undefined.
Pseudotyped particles of A(H1N1)pmd09 (A/California/7/2009) and HPAI H5N1 (A/Anhui/1/2005), as well as their reassortants, were generated. The reliability of using these pp in nAb detection were compared concurrently with the corresponding viruses by a hemagglutination inhibition test, as well as ELISA-, cytopathic effect-, and fluorescence-based microneutralization assays. In the qualitative detection on nAbs, the pp and their corresponding viruses were in complete agreement, with an R2 value equal to or near 1 in two different populations. In the quantitative detection on nAbs, although the geometric mean titers (95% confidence interval) differed between the pp and viruses, no significant difference was observed. Furthermore, humoral immunity against the reassortants was evaluated; our results indicated strong consistency between the nAbs against reassortant pp and those against naïve pp harboring the same hemagglutinin.
The pp displayed high reliability in influenza virus nAb detection. The use of reassortant pp is a safe and convenient strategy for characterizing emerging influenza viruses and surveying the disease burden.
Url:
DOI: 10.1371/journal.pone.0113629
PubMed: 25436460
PubMed Central: 4249968
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Reliability of Pseudotyped Influenza Viral Particles in Neutralizing Antibody Detection</title><author><name sortKey="Yang, Jinghui" sort="Yang, Jinghui" uniqKey="Yang J" first="Jinghui" last="Yang">Jinghui Yang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Li, Weidong" sort="Li, Weidong" uniqKey="Li W" first="Weidong" last="Li">Weidong Li</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Long, Yunfeng" sort="Long, Yunfeng" uniqKey="Long Y" first="Yunfeng" last="Long">Yunfeng Long</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Song, Shaohui" sort="Song, Shaohui" uniqKey="Song S" first="Shaohui" last="Song">Shaohui Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xinwen" sort="Zhang, Xinwen" uniqKey="Zhang X" first="Xinwen" last="Zhang">Xinwen Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiaoguang" sort="Wang, Xiaoguang" uniqKey="Wang X" first="Xiaoguang" last="Wang">Xiaoguang Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Minhang District Center for Disease Control and Prevention, Shanghai, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Minhang District Center for Disease Control and Prevention, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Shude" sort="Jiang, Shude" uniqKey="Jiang S" first="Shude" last="Jiang">Shude Jiang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Liao, Guoyang" sort="Liao, Guoyang" uniqKey="Liao G" first="Guoyang" last="Liao">Guoyang Liao</name><affiliation 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Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Li, Weidong" sort="Li, Weidong" uniqKey="Li W" first="Weidong" last="Li">Weidong Li</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Long, Yunfeng" sort="Long, Yunfeng" uniqKey="Long Y" first="Yunfeng" last="Long">Yunfeng Long</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Song, Shaohui" sort="Song, Shaohui" uniqKey="Song S" first="Shaohui" last="Song">Shaohui Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xinwen" sort="Zhang, Xinwen" uniqKey="Zhang X" first="Xinwen" last="Zhang">Xinwen Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiaoguang" sort="Wang, Xiaoguang" uniqKey="Wang X" first="Xiaoguang" last="Wang">Xiaoguang Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Minhang District Center for Disease Control and Prevention, Shanghai, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Minhang District Center for Disease Control and Prevention, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Shude" sort="Jiang, Shude" uniqKey="Jiang S" first="Shude" last="Jiang">Shude Jiang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author><author><name sortKey="Liao, Guoyang" sort="Liao, Guoyang" uniqKey="Liao G" first="Guoyang" last="Liao">Guoyang Liao</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan</wicri:regionArea><wicri:noRegion>Yunnan</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Current influenza control strategies require an active surveillance system. Pseudotyped viral particles (pp) together with the evaluation of pre-existing immunity in a population might satisfy this requirement. However, the reliability of using pp in neutralizing antibody (nAb) detection are undefined.</p></sec><sec><title>Methodology/Principal Findings</title><p>Pseudotyped particles of A(H1N1)pmd09 (A/California/7/2009) and HPAI H5N1 (A/Anhui/1/2005), as well as their reassortants, were generated. The reliability of using these pp in nAb detection were compared concurrently with the corresponding viruses by a hemagglutination inhibition test, as well as ELISA-, cytopathic effect-, and fluorescence-based microneutralization assays. In the qualitative detection on nAbs, the pp and their corresponding viruses were in complete agreement, with an R<sup>2</sup> value equal to or near 1 in two different populations. In the quantitative detection on nAbs, although the geometric mean titers (95% confidence interval) differed between the pp and viruses, no significant difference was observed. Furthermore, humoral immunity against the reassortants was evaluated; our results indicated strong consistency between the nAbs against reassortant pp and those against naïve pp harboring the same hemagglutinin.</p></sec><sec><title>Conclusion/Significance</title><p>The pp displayed high reliability in influenza virus nAb detection. The use of reassortant pp is a safe and convenient strategy for characterizing emerging influenza viruses and surveying the disease burden.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Dawood, Fs" uniqKey="Dawood F">FS Dawood</name></author><author><name sortKey="Iuliano, Ad" uniqKey="Iuliano A">AD Iuliano</name></author><author><name sortKey="Reed, C" uniqKey="Reed C">C Reed</name></author><author><name sortKey="Meltzer, Mi" uniqKey="Meltzer M">MI Meltzer</name></author><author><name sortKey="Shay, Dk" uniqKey="Shay D">DK Shay</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Tang, Z" uniqKey="Tang Z">Z Tang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Skountzou, I" uniqKey="Skountzou I">I Skountzou</name></author><author><name sortKey="Koutsonanos, Dg" uniqKey="Koutsonanos D">DG Koutsonanos</name></author><author><name sortKey="Kim, Jh" uniqKey="Kim J">JH Kim</name></author><author><name sortKey="Powers, R" uniqKey="Powers R">R Powers</name></author><author><name sortKey="Satyabhama, L" uniqKey="Satyabhama L">L Satyabhama</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Xing, Z" uniqKey="Xing Z">Z Xing</name></author><author><name sortKey="Cardona, Cj" uniqKey="Cardona C">CJ Cardona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, R" uniqKey="Xu R">R Xu</name></author><author><name sortKey="Ekiert, Dc" uniqKey="Ekiert D">DC Ekiert</name></author><author><name sortKey="Krause, Jc" uniqKey="Krause J">JC Krause</name></author><author><name sortKey="Hai, R" uniqKey="Hai R">R Hai</name></author><author><name sortKey="Crowe, Je" uniqKey="Crowe J">JE Crowe</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Hoofnagle, Jh" uniqKey="Hoofnagle J">JH Hoofnagle</name></author><author><name sortKey="Nelson, Ke" uniqKey="Nelson K">KE Nelson</name></author><author><name sortKey="Purcell, Rh" uniqKey="Purcell R">RH Purcell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, J" uniqKey="Lu J">J Lu</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Lin, X" uniqKey="Lin X">X Lin</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y Jiang</name></author><author><name sortKey="Tian, R" uniqKey="Tian R">R Tian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shu, Y" uniqKey="Shu Y">Y Shu</name></author><author><name sortKey="Yu, H" uniqKey="Yu H">H Yu</name></author><author><name sortKey="Li, D" uniqKey="Li D">D Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Lin, X" uniqKey="Lin X">X Lin</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Tan, W" uniqKey="Tan W">W Tan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Lin, X" uniqKey="Lin X">X Lin</name></author><author><name sortKey="Wang, G" uniqKey="Wang G">G Wang</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author><author><name sortKey="Lu, J" uniqKey="Lu J">J Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Xu, C" uniqKey="Xu C">C Xu</name></author><author><name sortKey="Song, J" uniqKey="Song J">J Song</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Cui, D" uniqKey="Cui D">D Cui</name></author><author><name sortKey="Yao, H" uniqKey="Yao H">H Yao</name></author><author><name sortKey="Lou, J" uniqKey="Lou J">J Lou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goff, Ph" uniqKey="Goff P">PH Goff</name></author><author><name sortKey="Krammer, F" uniqKey="Krammer F">F Krammer</name></author><author><name sortKey="Hai, R" uniqKey="Hai R">R Hai</name></author><author><name sortKey="Seibert, Cw" uniqKey="Seibert C">CW Seibert</name></author><author><name sortKey="Margine, I" uniqKey="Margine I">I Margine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boni, Mf" uniqKey="Boni M">MF Boni</name></author><author><name sortKey="Chau, Nv" uniqKey="Chau N">NV Chau</name></author><author><name sortKey="Dong, N" uniqKey="Dong N">N Dong</name></author><author><name sortKey="Todd, S" uniqKey="Todd S">S Todd</name></author><author><name sortKey="Nhat, Nt" uniqKey="Nhat N">NT Nhat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hancock, K" uniqKey="Hancock K">K Hancock</name></author><author><name sortKey="Veguilla, V" uniqKey="Veguilla V">V Veguilla</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author><author><name sortKey="Zhong, W" uniqKey="Zhong W">W Zhong</name></author><author><name sortKey="Butler, En" uniqKey="Butler E">EN Butler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Dong, B" uniqKey="Dong B">B Dong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Itoh, Y" uniqKey="Itoh Y">Y Itoh</name></author><author><name sortKey="Shinya, K" uniqKey="Shinya K">K Shinya</name></author><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name></author><author><name sortKey="Watanabe, T" uniqKey="Watanabe T">T Watanabe</name></author><author><name sortKey="Sakoda, Y" uniqKey="Sakoda Y">Y Sakoda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, E" uniqKey="Miller E">E Miller</name></author><author><name sortKey="Hoschler, K" uniqKey="Hoschler K">K Hoschler</name></author><author><name sortKey="Hardelid, P" uniqKey="Hardelid P">P Hardelid</name></author><author><name sortKey="Stanford, E" uniqKey="Stanford E">E Stanford</name></author><author><name sortKey="Andrews, N" uniqKey="Andrews N">N Andrews</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rizzo, C" uniqKey="Rizzo C">C Rizzo</name></author><author><name sortKey="Rota, Mc" uniqKey="Rota M">MC Rota</name></author><author><name sortKey="Bella, A" uniqKey="Bella A">A Bella</name></author><author><name sortKey="Alfonsi, V" uniqKey="Alfonsi V">V Alfonsi</name></author><author><name sortKey="Declich, S" uniqKey="Declich S">S Declich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chi, Cy" uniqKey="Chi C">CY Chi</name></author><author><name sortKey="Liu, Cc" uniqKey="Liu C">CC Liu</name></author><author><name sortKey="Lin, Cc" uniqKey="Lin C">CC Lin</name></author><author><name sortKey="Wang, Hc" uniqKey="Wang H">HC Wang</name></author><author><name sortKey="Cheng, Yt" uniqKey="Cheng Y">YT Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alberini, I" uniqKey="Alberini I">I Alberini</name></author><author><name sortKey="Del Tordello, E" uniqKey="Del Tordello E">E Del Tordello</name></author><author><name sortKey="Fasolo, A" uniqKey="Fasolo A">A Fasolo</name></author><author><name sortKey="Temperton, Nj" uniqKey="Temperton N">NJ Temperton</name></author><author><name sortKey="Galli, G" uniqKey="Galli G">G Galli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia, Jm" uniqKey="Garcia J">JM Garcia</name></author><author><name sortKey="Lagarde, N" uniqKey="Lagarde N">N Lagarde</name></author><author><name sortKey="Ma, Es" uniqKey="Ma E">ES Ma</name></author><author><name sortKey="De Jong, Md" uniqKey="De Jong M">MD de Jong</name></author><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Labrosse, B" uniqKey="Labrosse B">B Labrosse</name></author><author><name sortKey="Tourdjman, M" uniqKey="Tourdjman M">M Tourdjman</name></author><author><name sortKey="Porcher, R" uniqKey="Porcher R">R Porcher</name></author><author><name sortKey="Legoff, J" uniqKey="Legoff J">J LeGoff</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X de Lamballerie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gavrilov, V" uniqKey="Gavrilov V">V Gavrilov</name></author><author><name sortKey="Orekov, T" uniqKey="Orekov T">T Orekov</name></author><author><name sortKey="Alabanza, C" uniqKey="Alabanza C">C Alabanza</name></author><author><name sortKey="Porika, U" uniqKey="Porika U">U Porika</name></author><author><name sortKey="Jiang, H" uniqKey="Jiang H">H Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, C" uniqKey="Qiu C">C Qiu</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Zhang, A" uniqKey="Zhang A">A Zhang</name></author><author><name sortKey="Tian, D" uniqKey="Tian D">D Tian</name></author><author><name sortKey="Wan, Y" uniqKey="Wan Y">Y Wan</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yang, Jinghui" sort="Yang, Jinghui" uniqKey="Yang J" first="Jinghui" last="Yang">Jinghui Yang</name></noRegion><name sortKey="Jiang, Shude" sort="Jiang, Shude" uniqKey="Jiang S" first="Shude" last="Jiang">Shude Jiang</name><name sortKey="Li, Weidong" sort="Li, Weidong" uniqKey="Li W" first="Weidong" last="Li">Weidong Li</name><name sortKey="Liao, Guoyang" sort="Liao, Guoyang" uniqKey="Liao G" first="Guoyang" last="Liao">Guoyang Liao</name><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name><name sortKey="Long, Yunfeng" sort="Long, Yunfeng" uniqKey="Long Y" first="Yunfeng" last="Long">Yunfeng Long</name><name sortKey="Song, Shaohui" sort="Song, Shaohui" uniqKey="Song S" first="Shaohui" last="Song">Shaohui Song</name><name sortKey="Wang, Xiaoguang" sort="Wang, Xiaoguang" uniqKey="Wang X" first="Xiaoguang" last="Wang">Xiaoguang Wang</name><name sortKey="Zhang, Xinwen" sort="Zhang, Xinwen" uniqKey="Zhang X" first="Xinwen" last="Zhang">Xinwen Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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