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[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].

Identifieur interne : 000950 ( Ncbi/Checkpoint ); précédent : 000949; suivant : 000951

[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].

Auteurs : L A Stepanova ; A A Kovaleva ; M V Potapchuk ; A V Korotkov ; V V Kupriianov ; E A Blokhina ; R Iu Kotliarov ; L M Tsybalova

Source :

RBID : pubmed:24006628

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English descriptors

Abstract

Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses.

PubMed: 24006628


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pubmed:24006628

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<name sortKey="Kovaleva, A A" sort="Kovaleva, A A" uniqKey="Kovaleva A" first="A A" last="Kovaleva">A A Kovaleva</name>
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<name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name>
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<name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name>
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<name sortKey="Kupriianov, V V" sort="Kupriianov, V V" uniqKey="Kupriianov V" first="V V" last="Kupriianov">V V Kupriianov</name>
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<name sortKey="Kotliarov, R Iu" sort="Kotliarov, R Iu" uniqKey="Kotliarov R" first="R Iu" last="Kotliarov">R Iu Kotliarov</name>
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<keywords scheme="KwdEn" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Cross Protection</term>
<term>Flagellin (genetics)</term>
<term>Flagellin (immunology)</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin G (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H2N2 Subtype (genetics)</term>
<term>Influenza A Virus, H2N2 Subtype (immunology)</term>
<term>Influenza A Virus, H3N2 Subtype (genetics)</term>
<term>Influenza A Virus, H3N2 Subtype (immunology)</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza Vaccines (administration & dosage)</term>
<term>Influenza Vaccines (genetics)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Influenza, Human (virology)</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Orthomyxoviridae Infections (immunology)</term>
<term>Orthomyxoviridae Infections (prevention & control)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Protein Structure, Tertiary</term>
<term>Recombinant Fusion Proteins (administration & dosage)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Vaccination</term>
<term>Vaccines, Synthetic</term>
<term>Viral Load</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (immunology)</term>
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<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Charge virale</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Flagelline (génétique)</term>
<term>Flagelline (immunologie)</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (immunologie)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Immunoglobuline G (sang)</term>
<term>Infections à Orthomyxoviridae ()</term>
<term>Infections à Orthomyxoviridae (immunologie)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Protection croisée</term>
<term>Protéines de fusion recombinantes (administration et posologie)</term>
<term>Protéines de fusion recombinantes (génétique)</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (immunologie)</term>
<term>Souris</term>
<term>Sous-type H1N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H2N2 du virus de la grippe A (génétique)</term>
<term>Sous-type H2N2 du virus de la grippe A (immunologie)</term>
<term>Sous-type H3N2 du virus de la grippe A (génétique)</term>
<term>Sous-type H3N2 du virus de la grippe A (immunologie)</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Vaccination</term>
<term>Vaccins antigrippaux (administration et posologie)</term>
<term>Vaccins antigrippaux (génétique)</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins synthétiques</term>
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<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en">
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en">
<term>Antibodies, Viral</term>
<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Flagellin</term>
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en">
<term>Antibodies, Viral</term>
<term>Flagellin</term>
<term>Immunoglobulin G</term>
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr">
<term>Protéines de fusion recombinantes</term>
<term>Vaccins antigrippaux</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>Flagelline</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
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<term>Anticorps antiviraux</term>
<term>Flagelline</term>
<term>Grippe humaine</term>
<term>Immunoglobuline G</term>
<term>Infections à Orthomyxoviridae</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en">
<term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
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<term>Orthomyxoviridae Infections</term>
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<term>Orthomyxoviridae Infections</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr">
<term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr">
<term>Grippe humaine</term>
<term>Infections à Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en">
<term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cross Protection</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
<term>Vaccination</term>
<term>Vaccines, Synthetic</term>
<term>Viral Load</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Animaux</term>
<term>Charge virale</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Infections à Orthomyxoviridae</term>
<term>Protection croisée</term>
<term>Souris</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Vaccination</term>
<term>Vaccins synthétiques</term>
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<front>
<div type="abstract" xml:lang="en">Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses.</div>
</front>
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<name sortKey="Blokhina, E A" sort="Blokhina, E A" uniqKey="Blokhina E" first="E A" last="Blokhina">E A Blokhina</name>
<name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name>
<name sortKey="Kotliarov, R Iu" sort="Kotliarov, R Iu" uniqKey="Kotliarov R" first="R Iu" last="Kotliarov">R Iu Kotliarov</name>
<name sortKey="Kovaleva, A A" sort="Kovaleva, A A" uniqKey="Kovaleva A" first="A A" last="Kovaleva">A A Kovaleva</name>
<name sortKey="Kupriianov, V V" sort="Kupriianov, V V" uniqKey="Kupriianov V" first="V V" last="Kupriianov">V V Kupriianov</name>
<name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name>
<name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name>
<name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name>
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