[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].
Identifieur interne : 000950 ( Ncbi/Checkpoint ); précédent : 000949; suivant : 000951[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].
Auteurs : L A Stepanova ; A A Kovaleva ; M V Potapchuk ; A V Korotkov ; V V Kupriianov ; E A Blokhina ; R Iu Kotliarov ; L M TsybalovaSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Charge virale, Données de séquences moléculaires, Expression des gènes, Flagelline (génétique), Flagelline (immunologie), Grippe humaine (), Grippe humaine (immunologie), Grippe humaine (virologie), Humains, Immunoglobuline G (immunologie), Immunoglobuline G (sang), Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (immunologie), Infections à Orthomyxoviridae (virologie), Protection croisée, Protéines de fusion recombinantes (administration et posologie), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (immunologie), Protéines de la matrice virale (génétique), Protéines de la matrice virale (immunologie), Souris, Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (immunologie), Sous-type H3N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (immunologie), Structure tertiaire des protéines, Séquence d'acides aminés, Vaccination, Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins synthétiques.
- MESH :
- administration et posologie : Protéines de fusion recombinantes, Vaccins antigrippaux.
- génétique : Flagelline, Protéines de fusion recombinantes, Protéines de la matrice virale, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A, Vaccins antigrippaux.
- immunologie : Anticorps antiviraux, Flagelline, Grippe humaine, Immunoglobuline G, Infections à Orthomyxoviridae, Protéines de fusion recombinantes, Protéines de la matrice virale, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A, Vaccins antigrippaux.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Grippe humaine, Infections à Orthomyxoviridae.
- Animaux, Charge virale, Données de séquences moléculaires, Expression des gènes, Grippe humaine, Humains, Infections à Orthomyxoviridae, Protection croisée, Souris, Structure tertiaire des protéines, Séquence d'acides aminés, Vaccination, Vaccins synthétiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Cross Protection, Flagellin (genetics), Flagellin (immunology), Gene Expression, Humans, Immunoglobulin G (blood), Immunoglobulin G (immunology), Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (immunology), Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (immunology), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (immunology), Influenza Vaccines (administration & dosage), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Influenza, Human (immunology), Influenza, Human (prevention & control), Influenza, Human (virology), Mice, Molecular Sequence Data, Orthomyxoviridae Infections (immunology), Orthomyxoviridae Infections (prevention & control), Orthomyxoviridae Infections (virology), Protein Structure, Tertiary, Recombinant Fusion Proteins (administration & dosage), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Vaccination, Vaccines, Synthetic, Viral Load, Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
- MESH :
- chemical , administration & dosage : Influenza Vaccines, Recombinant Fusion Proteins.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Flagellin, Influenza Vaccines, Recombinant Fusion Proteins, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Flagellin, Immunoglobulin G, Influenza Vaccines, Recombinant Fusion Proteins, Viral Matrix Proteins.
- genetics : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype, Influenza, Human, Orthomyxoviridae Infections.
- prevention & control : Influenza, Human, Orthomyxoviridae Infections.
- virology : Influenza, Human, Orthomyxoviridae Infections.
- Amino Acid Sequence, Animals, Cross Protection, Gene Expression, Humans, Mice, Molecular Sequence Data, Protein Structure, Tertiary, Vaccination, Vaccines, Synthetic, Viral Load.
Abstract
Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses.
PubMed: 24006628
Affiliations:
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pubmed:24006628Le document en format XML
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<author><name sortKey="Kovaleva, A A" sort="Kovaleva, A A" uniqKey="Kovaleva A" first="A A" last="Kovaleva">A A Kovaleva</name>
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<author><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name>
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<author><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name>
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<author><name sortKey="Kupriianov, V V" sort="Kupriianov, V V" uniqKey="Kupriianov V" first="V V" last="Kupriianov">V V Kupriianov</name>
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<series><title level="j">Voprosy virusologii</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Cross Protection</term>
<term>Flagellin (genetics)</term>
<term>Flagellin (immunology)</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin G (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H2N2 Subtype (genetics)</term>
<term>Influenza A Virus, H2N2 Subtype (immunology)</term>
<term>Influenza A Virus, H3N2 Subtype (genetics)</term>
<term>Influenza A Virus, H3N2 Subtype (immunology)</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza Vaccines (administration & dosage)</term>
<term>Influenza Vaccines (genetics)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Influenza, Human (virology)</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Orthomyxoviridae Infections (immunology)</term>
<term>Orthomyxoviridae Infections (prevention & control)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Protein Structure, Tertiary</term>
<term>Recombinant Fusion Proteins (administration & dosage)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Vaccination</term>
<term>Vaccines, Synthetic</term>
<term>Viral Load</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Charge virale</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Flagelline (génétique)</term>
<term>Flagelline (immunologie)</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (immunologie)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Immunoglobuline G (sang)</term>
<term>Infections à Orthomyxoviridae ()</term>
<term>Infections à Orthomyxoviridae (immunologie)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Protection croisée</term>
<term>Protéines de fusion recombinantes (administration et posologie)</term>
<term>Protéines de fusion recombinantes (génétique)</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (immunologie)</term>
<term>Souris</term>
<term>Sous-type H1N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H2N2 du virus de la grippe A (génétique)</term>
<term>Sous-type H2N2 du virus de la grippe A (immunologie)</term>
<term>Sous-type H3N2 du virus de la grippe A (génétique)</term>
<term>Sous-type H3N2 du virus de la grippe A (immunologie)</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Vaccination</term>
<term>Vaccins antigrippaux (administration et posologie)</term>
<term>Vaccins antigrippaux (génétique)</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins synthétiques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Flagellin</term>
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Flagellin</term>
<term>Immunoglobulin G</term>
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Protéines de fusion recombinantes</term>
<term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Flagelline</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Flagelline</term>
<term>Grippe humaine</term>
<term>Immunoglobuline G</term>
<term>Infections à Orthomyxoviridae</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H2N2 Subtype</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Infections à Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cross Protection</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
<term>Vaccination</term>
<term>Vaccines, Synthetic</term>
<term>Viral Load</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Charge virale</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Infections à Orthomyxoviridae</term>
<term>Protection croisée</term>
<term>Souris</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Vaccination</term>
<term>Vaccins synthétiques</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en">Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses.</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Blokhina, E A" sort="Blokhina, E A" uniqKey="Blokhina E" first="E A" last="Blokhina">E A Blokhina</name>
<name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name>
<name sortKey="Kotliarov, R Iu" sort="Kotliarov, R Iu" uniqKey="Kotliarov R" first="R Iu" last="Kotliarov">R Iu Kotliarov</name>
<name sortKey="Kovaleva, A A" sort="Kovaleva, A A" uniqKey="Kovaleva A" first="A A" last="Kovaleva">A A Kovaleva</name>
<name sortKey="Kupriianov, V V" sort="Kupriianov, V V" uniqKey="Kupriianov V" first="V V" last="Kupriianov">V V Kupriianov</name>
<name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name>
<name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name>
<name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name>
</noCountry>
</tree>
</affiliations>
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