Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury
Identifieur interne : 000908 ( Ncbi/Checkpoint ); précédent : 000907; suivant : 000909Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury
Auteurs : Cristiana C. Garcia [Brésil] ; Wynne Weston-Davies [Royaume-Uni] ; Remo C. Russo [Brésil] ; Luciana P. Tavares [Brésil] ; Milene A. Rachid [Brésil] ; José C. Alves-Filho [Brésil] ; Alexandre V. Machado [Brésil] ; Bernhard Ryffel [Afrique du Sud] ; Miles A. Nunn [Royaume-Uni] ; Mauro M. Teixeira [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Influenza virus A (IAV) causes annual epidemics and intermittent pandemics that affect millions of people worldwide. Potent inflammatory responses are commonly associated with severe cases of IAV infection. The complement system, an important mechanism of innate and humoral immune responses to infections, is activated during primary IAV infection and mediates, in association with natural IgM, viral neutralization by virion aggregation and coating of viral hemmagglutinin. Increased levels of the anaphylatoxin C5a were found in patients fatally infected with the most recent H1N1 pandemic virus. In this study, our aim was to evaluate whether targeting C5 activation alters inflammatory lung injury and viral load in a murine model of IAV infection. To address this question C57Bl/6j mice were infected intranasally with 104 PFU of the mouse adapted Influenza A virus A/WSN/33 (H1N1) or inoculated with PBS (Mock). We demonstrated that C5a is increased in bronchoalveolar lavage fluid (BALF) upon experimental IAV infection. To evaluate the role of C5, we used OmCI, a potent arthropod-derived inhibitor of C5 activation that binds to C5 and prevents release of C5a by complement. OmCI was given daily by intraperitoneal injection from the day of IAV infection until day 5. Treatment with OmCI only partially reduced C5a levels in BALF. However, there was significant inhibition of neutrophil and macrophage infiltration in the airways, Neutrophil Extracellular Traps (NETs) formation, death of leukocytes, lung epithelial injury and overall lung damage induced by the infection. There was no effect on viral load. Taken together, these data suggest that targeting C5 activation with OmCI during IAV infection could be a promising approach to reduce excessive inflammatory reactions associated with the severe forms of IAV infections.
Url:
DOI: 10.1371/journal.pone.0064443
PubMed: 23696894
PubMed Central: 3655967
Affiliations:
- Afrique du Sud, Brésil, Royaume-Uni
- Angleterre, Grand Londres, Minas Gerais, État de São Paulo
- Belo Horizonte, Londres, São Paulo
- Université de São Paulo, Université fédérale du Minas Gerais
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000B62
- to stream Pmc, to step Curation: 000B62
- to stream Pmc, to step Checkpoint: 000639
- to stream Ncbi, to step Merge: 000908
- to stream Ncbi, to step Curation: 000908
Links to Exploration step
PMC:3655967Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury</title>
<author><name sortKey="Garcia, Cristiana C" sort="Garcia, Cristiana C" uniqKey="Garcia C" first="Cristiana C." last="Garcia">Cristiana C. Garcia</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Weston Davies, Wynne" sort="Weston Davies, Wynne" uniqKey="Weston Davies W" first="Wynne" last="Weston-Davies">Wynne Weston-Davies</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Varleigh Ltd, London, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Varleigh Ltd, London</wicri:regionArea>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Russo, Remo C" sort="Russo, Remo C" uniqKey="Russo R" first="Remo C." last="Russo">Remo C. Russo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Departamento de Fisiologia e Biofísica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Fisiologia e Biofísica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Tavares, Luciana P" sort="Tavares, Luciana P" uniqKey="Tavares L" first="Luciana P." last="Tavares">Luciana P. Tavares</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Rachid, Milene A" sort="Rachid, Milene A" uniqKey="Rachid M" first="Milene A." last="Rachid">Milene A. Rachid</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff4"><addr-line>Departamento de Patologia Geral, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Patologia Geral, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Alves Filho, Jose C" sort="Alves Filho, Jose C" uniqKey="Alves Filho J" first="José C." last="Alves-Filho">José C. Alves-Filho</name>
<affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Machado, Alexandre V" sort="Machado, Alexandre V" uniqKey="Machado A" first="Alexandre V." last="Machado">Alexandre V. Machado</name>
<affiliation wicri:level="2"><nlm:aff id="aff6"><addr-line>Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ryffel, Bernhard" sort="Ryffel, Bernhard" uniqKey="Ryffel B" first="Bernhard" last="Ryffel">Bernhard Ryffel</name>
<affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>CNRS UMR7355, CNRS and University Orleans, France and IIDMM, University of Cape Town, Cape Town, South Africa</addr-line>
</nlm:aff>
<country xml:lang="fr">Afrique du Sud</country>
<wicri:regionArea>CNRS UMR7355, CNRS and University Orleans, France and IIDMM, University of Cape Town, Cape Town</wicri:regionArea>
<wicri:noRegion>Cape Town</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunn, Miles A" sort="Nunn, Miles A" uniqKey="Nunn M" first="Miles A." last="Nunn">Miles A. Nunn</name>
<affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Centre for Ecology and Hydrology, Wallingford, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Ecology and Hydrology, Wallingford</wicri:regionArea>
<wicri:noRegion>Wallingford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Teixeira, Mauro M" sort="Teixeira, Mauro M" uniqKey="Teixeira M" first="Mauro M." last="Teixeira">Mauro M. Teixeira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23696894</idno>
<idno type="pmc">3655967</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655967</idno>
<idno type="RBID">PMC:3655967</idno>
<idno type="doi">10.1371/journal.pone.0064443</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000B62</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B62</idno>
<idno type="wicri:Area/Pmc/Curation">000B62</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B62</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000639</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000639</idno>
<idno type="wicri:Area/Ncbi/Merge">000908</idno>
<idno type="wicri:Area/Ncbi/Curation">000908</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000908</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury</title>
<author><name sortKey="Garcia, Cristiana C" sort="Garcia, Cristiana C" uniqKey="Garcia C" first="Cristiana C." last="Garcia">Cristiana C. Garcia</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Weston Davies, Wynne" sort="Weston Davies, Wynne" uniqKey="Weston Davies W" first="Wynne" last="Weston-Davies">Wynne Weston-Davies</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Varleigh Ltd, London, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Varleigh Ltd, London</wicri:regionArea>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Russo, Remo C" sort="Russo, Remo C" uniqKey="Russo R" first="Remo C." last="Russo">Remo C. Russo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Departamento de Fisiologia e Biofísica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Fisiologia e Biofísica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Tavares, Luciana P" sort="Tavares, Luciana P" uniqKey="Tavares L" first="Luciana P." last="Tavares">Luciana P. Tavares</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Rachid, Milene A" sort="Rachid, Milene A" uniqKey="Rachid M" first="Milene A." last="Rachid">Milene A. Rachid</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff4"><addr-line>Departamento de Patologia Geral, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Patologia Geral, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
<author><name sortKey="Alves Filho, Jose C" sort="Alves Filho, Jose C" uniqKey="Alves Filho J" first="José C." last="Alves-Filho">José C. Alves-Filho</name>
<affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Machado, Alexandre V" sort="Machado, Alexandre V" uniqKey="Machado A" first="Alexandre V." last="Machado">Alexandre V. Machado</name>
<affiliation wicri:level="2"><nlm:aff id="aff6"><addr-line>Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ryffel, Bernhard" sort="Ryffel, Bernhard" uniqKey="Ryffel B" first="Bernhard" last="Ryffel">Bernhard Ryffel</name>
<affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>CNRS UMR7355, CNRS and University Orleans, France and IIDMM, University of Cape Town, Cape Town, South Africa</addr-line>
</nlm:aff>
<country xml:lang="fr">Afrique du Sud</country>
<wicri:regionArea>CNRS UMR7355, CNRS and University Orleans, France and IIDMM, University of Cape Town, Cape Town</wicri:regionArea>
<wicri:noRegion>Cape Town</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunn, Miles A" sort="Nunn, Miles A" uniqKey="Nunn M" first="Miles A." last="Nunn">Miles A. Nunn</name>
<affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Centre for Ecology and Hydrology, Wallingford, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Ecology and Hydrology, Wallingford</wicri:regionArea>
<wicri:noRegion>Wallingford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Teixeira, Mauro M" sort="Teixeira, Mauro M" uniqKey="Teixeira M" first="Mauro M." last="Teixeira">Mauro M. Teixeira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
<settlement type="city">Belo Horizonte</settlement>
</placeName>
<orgName type="university">Université fédérale du Minas Gerais</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Influenza virus A (IAV) causes annual epidemics and intermittent pandemics that affect millions of people worldwide. Potent inflammatory responses are commonly associated with severe cases of IAV infection. The complement system, an important mechanism of innate and humoral immune responses to infections, is activated during primary IAV infection and mediates, in association with natural IgM, viral neutralization by virion aggregation and coating of viral hemmagglutinin. Increased levels of the anaphylatoxin C5a were found in patients fatally infected with the most recent H1N1 pandemic virus. In this study, our aim was to evaluate whether targeting C5 activation alters inflammatory lung injury and viral load in a murine model of IAV infection. To address this question C57Bl/6j mice were infected intranasally with 10<sup>4</sup>
PFU of the mouse adapted Influenza A virus A/WSN/33 (H1N1) or inoculated with PBS (Mock). We demonstrated that C5a is increased in bronchoalveolar lavage fluid (BALF) upon experimental IAV infection. To evaluate the role of C5, we used OmCI, a potent arthropod-derived inhibitor of C5 activation that binds to C5 and prevents release of C5a by complement. OmCI was given daily by intraperitoneal injection from the day of IAV infection until day 5. Treatment with OmCI only partially reduced C5a levels in BALF. However, there was significant inhibition of neutrophil and macrophage infiltration in the airways, Neutrophil Extracellular Traps (NETs) formation, death of leukocytes, lung epithelial injury and overall lung damage induced by the infection. There was no effect on viral load. Taken together, these data suggest that targeting C5 activation with OmCI during IAV infection could be a promising approach to reduce excessive inflammatory reactions associated with the severe forms of IAV infections.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Nascimento, Ej" uniqKey="Nascimento E">EJ Nascimento</name>
</author>
<author><name sortKey="Silva, Am" uniqKey="Silva A">AM Silva</name>
</author>
<author><name sortKey="Cordeiro, Mt" uniqKey="Cordeiro M">MT Cordeiro</name>
</author>
<author><name sortKey="Brito, Ca" uniqKey="Brito C">CA Brito</name>
</author>
<author><name sortKey="Gil, Lh" uniqKey="Gil L">LH Gil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kanmura, S" uniqKey="Kanmura S">S Kanmura</name>
</author>
<author><name sortKey="Uto, H" uniqKey="Uto H">H Uto</name>
</author>
<author><name sortKey="Sato, Y" uniqKey="Sato Y">Y Sato</name>
</author>
<author><name sortKey="Kumagai, K" uniqKey="Kumagai K">K Kumagai</name>
</author>
<author><name sortKey="Sasaki, F" uniqKey="Sasaki F">F Sasaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huber Lang, M" uniqKey="Huber Lang M">M Huber-Lang</name>
</author>
<author><name sortKey="Sarma, Vj" uniqKey="Sarma V">VJ Sarma</name>
</author>
<author><name sortKey="Lu, Kt" uniqKey="Lu K">KT Lu</name>
</author>
<author><name sortKey="Mcguire, Sr" uniqKey="Mcguire S">SR McGuire</name>
</author>
<author><name sortKey="Padgaonkar, Va" uniqKey="Padgaonkar V">VA Padgaonkar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvier, Nm" uniqKey="Bouvier N">NM Bouvier</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bjornson, Ab" uniqKey="Bjornson A">AB Bjornson</name>
</author>
<author><name sortKey="Mellencamp, Ma" uniqKey="Mellencamp M">MA Mellencamp</name>
</author>
<author><name sortKey="Schiff, Gm" uniqKey="Schiff G">GM Schiff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jayasekera, Jp" uniqKey="Jayasekera J">JP Jayasekera</name>
</author>
<author><name sortKey="Moseman, Ea" uniqKey="Moseman E">EA Moseman</name>
</author>
<author><name sortKey="Carroll, Mc" uniqKey="Carroll M">MC Carroll</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name>
</author>
<author><name sortKey="Li, G" uniqKey="Li G">G Li</name>
</author>
<author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name>
</author>
<author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
<author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="La Gruta, Nl" uniqKey="La Gruta N">NL La Gruta</name>
</author>
<author><name sortKey="Kedzierska, K" uniqKey="Kedzierska K">K Kedzierska</name>
</author>
<author><name sortKey="Stambas, J" uniqKey="Stambas J">J Stambas</name>
</author>
<author><name sortKey="Doherty, Pc" uniqKey="Doherty P">PC Doherty</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="O Brien, Kb" uniqKey="O Brien K">KB O'Brien</name>
</author>
<author><name sortKey="Morrison, Te" uniqKey="Morrison T">TE Morrison</name>
</author>
<author><name sortKey="Dundore, Dy" uniqKey="Dundore D">DY Dundore</name>
</author>
<author><name sortKey="Heise, Mt" uniqKey="Heise M">MT Heise</name>
</author>
<author><name sortKey="Schultz Cherry, S" uniqKey="Schultz Cherry S">S Schultz-Cherry</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guo, Rf" uniqKey="Guo R">RF Guo</name>
</author>
<author><name sortKey="Ward, Pa" uniqKey="Ward P">PA Ward</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia, Cc" uniqKey="Garcia C">CC Garcia</name>
</author>
<author><name sortKey="Russo, Rc" uniqKey="Russo R">RC Russo</name>
</author>
<author><name sortKey="Guabiraba, R" uniqKey="Guabiraba R">R Guabiraba</name>
</author>
<author><name sortKey="Fagundes, Ct" uniqKey="Fagundes C">CT Fagundes</name>
</author>
<author><name sortKey="Polidoro, Rb" uniqKey="Polidoro R">RB Polidoro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perrone, La" uniqKey="Perrone L">LA Perrone</name>
</author>
<author><name sortKey="Plowden, Jk" uniqKey="Plowden J">JK Plowden</name>
</author>
<author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name>
</author>
<author><name sortKey="Katz, Jm" uniqKey="Katz J">JM Katz</name>
</author>
<author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nataf, S" uniqKey="Nataf S">S Nataf</name>
</author>
<author><name sortKey="Davoust, N" uniqKey="Davoust N">N Davoust</name>
</author>
<author><name sortKey="Ames, Rs" uniqKey="Ames R">RS Ames</name>
</author>
<author><name sortKey="Barnum, Sr" uniqKey="Barnum S">SR Barnum</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kwan, Wh" uniqKey="Kwan W">WH Kwan</name>
</author>
<author><name sortKey="Van Der Touw, W" uniqKey="Van Der Touw W">W van der Touw</name>
</author>
<author><name sortKey="Paz Artal, E" uniqKey="Paz Artal E">E Paz-Artal</name>
</author>
<author><name sortKey="Li, Mo" uniqKey="Li M">MO Li</name>
</author>
<author><name sortKey="Heeger, Ps" uniqKey="Heeger P">PS Heeger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woolhouse, Me" uniqKey="Woolhouse M">ME Woolhouse</name>
</author>
<author><name sortKey="Webster, Jp" uniqKey="Webster J">JP Webster</name>
</author>
<author><name sortKey="Domingo, E" uniqKey="Domingo E">E Domingo</name>
</author>
<author><name sortKey="Charlesworth, B" uniqKey="Charlesworth B">B Charlesworth</name>
</author>
<author><name sortKey="Levin, Br" uniqKey="Levin B">BR Levin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hepburn, Nj" uniqKey="Hepburn N">NJ Hepburn</name>
</author>
<author><name sortKey="Williams, As" uniqKey="Williams A">AS Williams</name>
</author>
<author><name sortKey="Nunn, Ma" uniqKey="Nunn M">MA Nunn</name>
</author>
<author><name sortKey="Chamberlain Banoub, Jc" uniqKey="Chamberlain Banoub J">JC Chamberlain-Banoub</name>
</author>
<author><name sortKey="Hamer, J" uniqKey="Hamer J">J Hamer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nunn, Ma" uniqKey="Nunn M">MA Nunn</name>
</author>
<author><name sortKey="Sharma, A" uniqKey="Sharma A">A Sharma</name>
</author>
<author><name sortKey="Paesen, Gc" uniqKey="Paesen G">GC Paesen</name>
</author>
<author><name sortKey="Adamson, S" uniqKey="Adamson S">S Adamson</name>
</author>
<author><name sortKey="Lissina, O" uniqKey="Lissina O">O Lissina</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Soltys, J" uniqKey="Soltys J">J Soltys</name>
</author>
<author><name sortKey="Kusner, Ll" uniqKey="Kusner L">LL Kusner</name>
</author>
<author><name sortKey="Young, A" uniqKey="Young A">A Young</name>
</author>
<author><name sortKey="Richmonds, C" uniqKey="Richmonds C">C Richmonds</name>
</author>
<author><name sortKey="Hatala, D" uniqKey="Hatala D">D Hatala</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Halstead, Sk" uniqKey="Halstead S">SK Halstead</name>
</author>
<author><name sortKey="Humphreys, Pd" uniqKey="Humphreys P">PD Humphreys</name>
</author>
<author><name sortKey="Zitman, Fm" uniqKey="Zitman F">FM Zitman</name>
</author>
<author><name sortKey="Hamer, J" uniqKey="Hamer J">J Hamer</name>
</author>
<author><name sortKey="Plomp, Jj" uniqKey="Plomp J">JJ Plomp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barratt Due, A" uniqKey="Barratt Due A">A Barratt-Due</name>
</author>
<author><name sortKey="Thorgersen, Eb" uniqKey="Thorgersen E">EB Thorgersen</name>
</author>
<author><name sortKey="Lindstad, Jk" uniqKey="Lindstad J">JK Lindstad</name>
</author>
<author><name sortKey="Pharo, A" uniqKey="Pharo A">A Pharo</name>
</author>
<author><name sortKey="Lissina, O" uniqKey="Lissina O">O Lissina</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Narasaraju, T" uniqKey="Narasaraju T">T Narasaraju</name>
</author>
<author><name sortKey="Ng, Hh" uniqKey="Ng H">HH Ng</name>
</author>
<author><name sortKey="Phoon, Mc" uniqKey="Phoon M">MC Phoon</name>
</author>
<author><name sortKey="Chow, Vt" uniqKey="Chow V">VT Chow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Russo, Rc" uniqKey="Russo R">RC Russo</name>
</author>
<author><name sortKey="Guabiraba, R" uniqKey="Guabiraba R">R Guabiraba</name>
</author>
<author><name sortKey="Garcia, Cc" uniqKey="Garcia C">CC Garcia</name>
</author>
<author><name sortKey="Barcelos, Ls" uniqKey="Barcelos L">LS Barcelos</name>
</author>
<author><name sortKey="Roffe, E" uniqKey="Roffe E">E Roffe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Horvat, Jc" uniqKey="Horvat J">JC Horvat</name>
</author>
<author><name sortKey="Beagley, Kw" uniqKey="Beagley K">KW Beagley</name>
</author>
<author><name sortKey="Wade, Ma" uniqKey="Wade M">MA Wade</name>
</author>
<author><name sortKey="Preston, Ja" uniqKey="Preston J">JA Preston</name>
</author>
<author><name sortKey="Hansbro, Ng" uniqKey="Hansbro N">NG Hansbro</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Lin, Kl" uniqKey="Lin K">KL Lin</name>
</author>
<author><name sortKey="Suzuki, Y" uniqKey="Suzuki Y">Y Suzuki</name>
</author>
<author><name sortKey="Nakano, H" uniqKey="Nakano H">H Nakano</name>
</author>
<author><name sortKey="Ramsburg, E" uniqKey="Ramsburg E">E Ramsburg</name>
</author>
<author><name sortKey="Gunn, Md" uniqKey="Gunn M">MD Gunn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Papayannopoulos, V" uniqKey="Papayannopoulos V">V Papayannopoulos</name>
</author>
<author><name sortKey="Zychlinsky, A" uniqKey="Zychlinsky A">A Zychlinsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Logters, T" uniqKey="Logters T">T Logters</name>
</author>
<author><name sortKey="Margraf, S" uniqKey="Margraf S">S Margraf</name>
</author>
<author><name sortKey="Altrichter, J" uniqKey="Altrichter J">J Altrichter</name>
</author>
<author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
<author><name sortKey="Mitzner, S" uniqKey="Mitzner S">S Mitzner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Douda, Dn" uniqKey="Douda D">DN Douda</name>
</author>
<author><name sortKey="Jackson, R" uniqKey="Jackson R">R Jackson</name>
</author>
<author><name sortKey="Grasemann, H" uniqKey="Grasemann H">H Grasemann</name>
</author>
<author><name sortKey="Palaniyar, N" uniqKey="Palaniyar N">N Palaniyar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pisetsky, Ds" uniqKey="Pisetsky D">DS Pisetsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tran, At" uniqKey="Tran A">AT Tran</name>
</author>
<author><name sortKey="Cortens, Jp" uniqKey="Cortens J">JP Cortens</name>
</author>
<author><name sortKey="Du, Q" uniqKey="Du Q">Q Du</name>
</author>
<author><name sortKey="Wilkins, Ja" uniqKey="Wilkins J">JA Wilkins</name>
</author>
<author><name sortKey="Coombs, Km" uniqKey="Coombs K">KM Coombs</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kopf, M" uniqKey="Kopf M">M Kopf</name>
</author>
<author><name sortKey="Abel, B" uniqKey="Abel B">B Abel</name>
</author>
<author><name sortKey="Gallimore, A" uniqKey="Gallimore A">A Gallimore</name>
</author>
<author><name sortKey="Carroll, M" uniqKey="Carroll M">M Carroll</name>
</author>
<author><name sortKey="Bachmann, Mf" uniqKey="Bachmann M">MF Bachmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, Ah" uniqKey="Kim A">AH Kim</name>
</author>
<author><name sortKey="Dimitriou, Id" uniqKey="Dimitriou I">ID Dimitriou</name>
</author>
<author><name sortKey="Holland, Mc" uniqKey="Holland M">MC Holland</name>
</author>
<author><name sortKey="Mastellos, D" uniqKey="Mastellos D">D Mastellos</name>
</author>
<author><name sortKey="Mueller, Ym" uniqKey="Mueller Y">YM Mueller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name>
</author>
<author><name sortKey="Mitamura, K" uniqKey="Mitamura K">K Mitamura</name>
</author>
<author><name sortKey="Sakai Tagawa, Y" uniqKey="Sakai Tagawa Y">Y Sakai-Tagawa</name>
</author>
<author><name sortKey="Shiraishi, K" uniqKey="Shiraishi K">K Shiraishi</name>
</author>
<author><name sortKey="Kawakami, C" uniqKey="Kawakami C">C Kawakami</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name>
</author>
<author><name sortKey="Chan, Kw" uniqKey="Chan K">KW Chan</name>
</author>
<author><name sortKey="Lin, Yp" uniqKey="Lin Y">YP Lin</name>
</author>
<author><name sortKey="Zhao, Gy" uniqKey="Zhao G">GY Zhao</name>
</author>
<author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kobasa, D" uniqKey="Kobasa D">D Kobasa</name>
</author>
<author><name sortKey="Takada, A" uniqKey="Takada A">A Takada</name>
</author>
<author><name sortKey="Shinya, K" uniqKey="Shinya K">K Shinya</name>
</author>
<author><name sortKey="Hatta, M" uniqKey="Hatta M">M Hatta</name>
</author>
<author><name sortKey="Halfmann, P" uniqKey="Halfmann P">P Halfmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lim, H" uniqKey="Lim H">H Lim</name>
</author>
<author><name sortKey="Kim, Yu" uniqKey="Kim Y">YU Kim</name>
</author>
<author><name sortKey="Drouin, Sm" uniqKey="Drouin S">SM Drouin</name>
</author>
<author><name sortKey="Mueller Ortiz, S" uniqKey="Mueller Ortiz S">S Mueller-Ortiz</name>
</author>
<author><name sortKey="Yun, K" uniqKey="Yun K">K Yun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huber Lang, M" uniqKey="Huber Lang M">M Huber-Lang</name>
</author>
<author><name sortKey="Sarma, Jv" uniqKey="Sarma J">JV Sarma</name>
</author>
<author><name sortKey="Zetoune, Fs" uniqKey="Zetoune F">FS Zetoune</name>
</author>
<author><name sortKey="Rittirsch, D" uniqKey="Rittirsch D">D Rittirsch</name>
</author>
<author><name sortKey="Neff, Ta" uniqKey="Neff T">TA Neff</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Yousefi, S" uniqKey="Yousefi S">S Yousefi</name>
</author>
<author><name sortKey="Mihalache, C" uniqKey="Mihalache C">C Mihalache</name>
</author>
<author><name sortKey="Kozlowski, E" uniqKey="Kozlowski E">E Kozlowski</name>
</author>
<author><name sortKey="Schmid, I" uniqKey="Schmid I">I Schmid</name>
</author>
<author><name sortKey="Simon, Hu" uniqKey="Simon H">HU Simon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Narasaraju, T" uniqKey="Narasaraju T">T Narasaraju</name>
</author>
<author><name sortKey="Yang, E" uniqKey="Yang E">E Yang</name>
</author>
<author><name sortKey="Samy, Rp" uniqKey="Samy R">RP Samy</name>
</author>
<author><name sortKey="Ng, Hh" uniqKey="Ng H">HH Ng</name>
</author>
<author><name sortKey="Poh, Wp" uniqKey="Poh W">WP Poh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name>
</author>
<author><name sortKey="Park, Pw" uniqKey="Park P">PW Park</name>
</author>
<author><name sortKey="Wilson, Cl" uniqKey="Wilson C">CL Wilson</name>
</author>
<author><name sortKey="Parks, Wc" uniqKey="Parks W">WC Parks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dunkelberger, Jr" uniqKey="Dunkelberger J">JR Dunkelberger</name>
</author>
<author><name sortKey="Song, Wc" uniqKey="Song W">WC Song</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia, Cc" uniqKey="Garcia C">CC Garcia</name>
</author>
<author><name sortKey="Guabiraba, R" uniqKey="Guabiraba R">R Guabiraba</name>
</author>
<author><name sortKey="Soriani, Fm" uniqKey="Soriani F">FM Soriani</name>
</author>
<author><name sortKey="Teixeira, Mm" uniqKey="Teixeira M">MM Teixeira</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martin, El" uniqKey="Martin E">EL Martin</name>
</author>
<author><name sortKey="Souza, Dg" uniqKey="Souza D">DG Souza</name>
</author>
<author><name sortKey="Fagundes, Ct" uniqKey="Fagundes C">CT Fagundes</name>
</author>
<author><name sortKey="Amaral, Fa" uniqKey="Amaral F">FA Amaral</name>
</author>
<author><name sortKey="Assenzio, B" uniqKey="Assenzio B">B Assenzio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Souza, Dg" uniqKey="Souza D">DG Souza</name>
</author>
<author><name sortKey="Fagundes, Ct" uniqKey="Fagundes C">CT Fagundes</name>
</author>
<author><name sortKey="Sousa, Lp" uniqKey="Sousa L">LP Sousa</name>
</author>
<author><name sortKey="Amaral, Fa" uniqKey="Amaral F">FA Amaral</name>
</author>
<author><name sortKey="Souza, Rs" uniqKey="Souza R">RS Souza</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lacerda Queiroz, N" uniqKey="Lacerda Queiroz N">N Lacerda-Queiroz</name>
</author>
<author><name sortKey="Rodrigues, Dh" uniqKey="Rodrigues D">DH Rodrigues</name>
</author>
<author><name sortKey="Vilela, Mc" uniqKey="Vilela M">MC Vilela</name>
</author>
<author><name sortKey="Rachid, Ma" uniqKey="Rachid M">MA Rachid</name>
</author>
<author><name sortKey="Soriani, Fm" uniqKey="Soriani F">FM Soriani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Longhi, Mp" uniqKey="Longhi M">MP Longhi</name>
</author>
<author><name sortKey="Williams, A" uniqKey="Williams A">A Williams</name>
</author>
<author><name sortKey="Wise, M" uniqKey="Wise M">M Wise</name>
</author>
<author><name sortKey="Morgan, Bp" uniqKey="Morgan B">BP Morgan</name>
</author>
<author><name sortKey="Gallimore, A" uniqKey="Gallimore A">A Gallimore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sanderson, Sd" uniqKey="Sanderson S">SD Sanderson</name>
</author>
<author><name sortKey="Thoman, Ml" uniqKey="Thoman M">ML Thoman</name>
</author>
<author><name sortKey="Kis, K" uniqKey="Kis K">K Kis</name>
</author>
<author><name sortKey="Virts, El" uniqKey="Virts E">EL Virts</name>
</author>
<author><name sortKey="Herrera, Eb" uniqKey="Herrera E">EB Herrera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Damjanovic, D" uniqKey="Damjanovic D">D Damjanovic</name>
</author>
<author><name sortKey="Small, Cl" uniqKey="Small C">CL Small</name>
</author>
<author><name sortKey="Jeyananthan, M" uniqKey="Jeyananthan M">M Jeyananthan</name>
</author>
<author><name sortKey="Mccormick, S" uniqKey="Mccormick S">S McCormick</name>
</author>
<author><name sortKey="Xing, Z" uniqKey="Xing Z">Z Xing</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Afrique du Sud</li>
<li>Brésil</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Grand Londres</li>
<li>Minas Gerais</li>
<li>État de São Paulo</li>
</region>
<settlement><li>Belo Horizonte</li>
<li>Londres</li>
<li>São Paulo</li>
</settlement>
<orgName><li>Université de São Paulo</li>
<li>Université fédérale du Minas Gerais</li>
</orgName>
</list>
<tree><country name="Brésil"><region name="Minas Gerais"><name sortKey="Garcia, Cristiana C" sort="Garcia, Cristiana C" uniqKey="Garcia C" first="Cristiana C." last="Garcia">Cristiana C. Garcia</name>
</region>
<name sortKey="Alves Filho, Jose C" sort="Alves Filho, Jose C" uniqKey="Alves Filho J" first="José C." last="Alves-Filho">José C. Alves-Filho</name>
<name sortKey="Machado, Alexandre V" sort="Machado, Alexandre V" uniqKey="Machado A" first="Alexandre V." last="Machado">Alexandre V. Machado</name>
<name sortKey="Rachid, Milene A" sort="Rachid, Milene A" uniqKey="Rachid M" first="Milene A." last="Rachid">Milene A. Rachid</name>
<name sortKey="Rachid, Milene A" sort="Rachid, Milene A" uniqKey="Rachid M" first="Milene A." last="Rachid">Milene A. Rachid</name>
<name sortKey="Russo, Remo C" sort="Russo, Remo C" uniqKey="Russo R" first="Remo C." last="Russo">Remo C. Russo</name>
<name sortKey="Russo, Remo C" sort="Russo, Remo C" uniqKey="Russo R" first="Remo C." last="Russo">Remo C. Russo</name>
<name sortKey="Tavares, Luciana P" sort="Tavares, Luciana P" uniqKey="Tavares L" first="Luciana P." last="Tavares">Luciana P. Tavares</name>
<name sortKey="Teixeira, Mauro M" sort="Teixeira, Mauro M" uniqKey="Teixeira M" first="Mauro M." last="Teixeira">Mauro M. Teixeira</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Weston Davies, Wynne" sort="Weston Davies, Wynne" uniqKey="Weston Davies W" first="Wynne" last="Weston-Davies">Wynne Weston-Davies</name>
</region>
<name sortKey="Nunn, Miles A" sort="Nunn, Miles A" uniqKey="Nunn M" first="Miles A." last="Nunn">Miles A. Nunn</name>
</country>
<country name="Afrique du Sud"><noRegion><name sortKey="Ryffel, Bernhard" sort="Ryffel, Bernhard" uniqKey="Ryffel B" first="Bernhard" last="Ryffel">Bernhard Ryffel</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000908 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000908 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= PMC:3655967 |texte= Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:23696894" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |