The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses
Identifieur interne : 002543 ( Main/Merge ); précédent : 002542; suivant : 002544The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses
Auteurs : C. Scholtissek [Allemagne] ; H. Bürger [Allemagne] ; O. Kistner [Allemagne] ; K. F. Shortridge [Hong Kong]Source :
- Virology [ 0042-6822 ] ; 1985.
English descriptors
- Teeft :
- Academic press, Antigenic, Antigenic shift, Antigenic variation, Avian, Avian influenza, Avian origin, Avian strains, Avian viruses, Cell culture, Different species, Double infection, Fowl plague, Fowl plague virus, Gene constellation, Genes coding, Genetic relatedness, Geographical region, Hong kong, Host specificity, Human strains, Hybridization, Hybridization technique, Identical viruses, Influenza, Influenza viruses, Interspecies transmission, Kistner, Lysis buffer, Monoclonal antibodies, Mutant, Nonlabeled crna, Nucleoprotein, Optical density, Page patterns, Pandemic influenza, Phosphopeptide, Phosphopeptide fingerprints, Porcine, Porcine viruses, Rescue experiments, Rnase resistance, Rott, Scholtissek, Shortridge, Southern china, Species barrier, Squirrel monkey, Surface glycoproteins, Swine, Swine viruses, Tissue tropism, Unpublished results, Virol privalsky, Virology, Virology scholtissek, Virus, Virus strains, Vrna segment.
Abstract
Abstract: In an attempt to assess the importance of the nucleoprotein (NP) in the determination of host specificity, a series of experiments was performed on influenza A viruses of the H3N2 subtype. We have examined rescue of mutants of A/FPV/Rostock/34 with temperature-sensitive (ts) lesions in the nucleoprotein (NP) gene by double infection of chick embryo cells with H3N2 strains isolated from different species. The is mutants could be rescued by all avian H3N2 strains but not by any of the human H3N2 isolates. Only two of the swine H3N2 strains tested were able to rescue our mutants. The NP gene of these two swine isolates resembled the NP gene of the avian strains genetically in the hybridization test. However, their NPs reacted differently with a set of monoclonal antibodies when compared with NPs of avian H3N2 strains. Concerning multiplication in ducks they behaved like the other swine and human strains. The phosphopeptide fingerprints of all swine isolates tested were alike and were different from those of human or avian origin. Our observations are compatible with the idea that human H3N2 strains might not be able to cross the species barrier to birds directly, and possibly also not the other way around, without prior reassortment in pigs, which seem to have a broader host range concerning the compatibility of the NP gene in reassortants.
Url:
DOI: 10.1016/0042-6822(85)90131-X
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Kistner</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut fur Virologie, Justus-Liebig-Universität Giessen, Giessen D-6300</wicri:regionArea><wicri:noRegion>Giessen D-6300</wicri:noRegion><wicri:noRegion>Giessen D-6300</wicri:noRegion></affiliation></author><author><name sortKey="Shortridge, K F" sort="Shortridge, K F" uniqKey="Shortridge K" first="K. F." last="Shortridge">K. F. Shortridge</name><affiliation wicri:level="1"><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Department of Microbiology, University of Hong Kong</wicri:regionArea><wicri:noRegion>University of Hong Kong</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">147</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="287">287</biblScope><biblScope unit="page" to="294">294</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Antigenic</term><term>Antigenic shift</term><term>Antigenic variation</term><term>Avian</term><term>Avian influenza</term><term>Avian origin</term><term>Avian strains</term><term>Avian viruses</term><term>Cell culture</term><term>Different species</term><term>Double infection</term><term>Fowl plague</term><term>Fowl plague virus</term><term>Gene constellation</term><term>Genes coding</term><term>Genetic relatedness</term><term>Geographical region</term><term>Hong kong</term><term>Host specificity</term><term>Human strains</term><term>Hybridization</term><term>Hybridization technique</term><term>Identical viruses</term><term>Influenza</term><term>Influenza viruses</term><term>Interspecies transmission</term><term>Kistner</term><term>Lysis buffer</term><term>Monoclonal antibodies</term><term>Mutant</term><term>Nonlabeled crna</term><term>Nucleoprotein</term><term>Optical density</term><term>Page patterns</term><term>Pandemic influenza</term><term>Phosphopeptide</term><term>Phosphopeptide fingerprints</term><term>Porcine</term><term>Porcine viruses</term><term>Rescue experiments</term><term>Rnase resistance</term><term>Rott</term><term>Scholtissek</term><term>Shortridge</term><term>Southern china</term><term>Species barrier</term><term>Squirrel monkey</term><term>Surface glycoproteins</term><term>Swine</term><term>Swine viruses</term><term>Tissue tropism</term><term>Unpublished results</term><term>Virol privalsky</term><term>Virology</term><term>Virology scholtissek</term><term>Virus</term><term>Virus strains</term><term>Vrna segment</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In an attempt to assess the importance of the nucleoprotein (NP) in the determination of host specificity, a series of experiments was performed on influenza A viruses of the H3N2 subtype. We have examined rescue of mutants of A/FPV/Rostock/34 with temperature-sensitive (ts) lesions in the nucleoprotein (NP) gene by double infection of chick embryo cells with H3N2 strains isolated from different species. The is mutants could be rescued by all avian H3N2 strains but not by any of the human H3N2 isolates. Only two of the swine H3N2 strains tested were able to rescue our mutants. The NP gene of these two swine isolates resembled the NP gene of the avian strains genetically in the hybridization test. However, their NPs reacted differently with a set of monoclonal antibodies when compared with NPs of avian H3N2 strains. Concerning multiplication in ducks they behaved like the other swine and human strains. The phosphopeptide fingerprints of all swine isolates tested were alike and were different from those of human or avian origin. Our observations are compatible with the idea that human H3N2 strains might not be able to cross the species barrier to birds directly, and possibly also not the other way around, without prior reassortment in pigs, which seem to have a broader host range concerning the compatibility of the NP gene in reassortants.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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