Antibody response to the M2 protein of influenza A virus expressed in insect cells.
Identifieur interne : 001F42 ( Main/Merge ); précédent : 001F41; suivant : 001F43Antibody response to the M2 protein of influenza A virus expressed in insect cells.
Auteurs : R A Black [États-Unis] ; P A Rota ; N. Gorodkova ; H D Klenk ; A P KendalSource :
- The Journal of general virology [ 0022-1317 ] ; 1993.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Anticorps monoclonaux, Données de séquences moléculaires, Furets, Humains, Papillons de nuit, Protéines de la matrice virale (immunologie), Protéines recombinantes (immunologie), Séquence nucléotidique, Technique de Western, Techniques immunoenzymatiques, Virus de la grippe A (immunologie).
- MESH :
- immunologie : Protéines de la matrice virale, Protéines recombinantes, Virus de la grippe A.
- sang : Anticorps antiviraux.
- Animaux, Anticorps monoclonaux, Données de séquences moléculaires, Furets, Humains, Papillons de nuit, Séquence nucléotidique, Technique de Western, Techniques immunoenzymatiques.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Recombinant Proteins, Viral Matrix Proteins.
- chemical : Antibodies, Monoclonal.
- immunology : Influenza A virus.
- Animals, Base Sequence, Blotting, Western, Ferrets, Humans, Immunoenzyme Techniques, Molecular Sequence Data, Moths.
Abstract
A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS-polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses.
DOI: 10.1099/0022-1317-74-1-143
PubMed: 8423445
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pubmed:8423445Le document en format XML
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Gorodkova</name></author><author><name sortKey="Klenk, H D" sort="Klenk, H D" uniqKey="Klenk H" first="H D" last="Klenk">H D Klenk</name></author><author><name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8423445</idno><idno type="pmid">8423445</idno><idno type="doi">10.1099/0022-1317-74-1-143</idno><idno type="wicri:Area/PubMed/Corpus">000429</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000429</idno><idno type="wicri:Area/PubMed/Curation">000429</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000429</idno><idno type="wicri:Area/PubMed/Checkpoint">000386</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000386</idno><idno type="wicri:Area/Ncbi/Merge">001320</idno><idno type="wicri:Area/Ncbi/Curation">001320</idno><idno type="wicri:Area/Ncbi/Checkpoint">001320</idno><idno type="wicri:doubleKey">0022-1317:1993:Black R:antibody:response:to</idno><idno type="wicri:Area/Main/Merge">001F42</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Antibody response to the M2 protein of influenza A virus expressed in insect cells.</title><author><name sortKey="Black, R A" sort="Black, R A" uniqKey="Black R" first="R A" last="Black">R A Black</name><affiliation wicri:level="2"><nlm:affiliation>Influenza Branch, Centers for Disease Control, Atlanta, Georgia 30333.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Branch, Centers for Disease Control, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Rota, P A" sort="Rota, P A" uniqKey="Rota P" first="P A" last="Rota">P A Rota</name></author><author><name sortKey="Gorodkova, N" sort="Gorodkova, N" uniqKey="Gorodkova N" first="N" last="Gorodkova">N. Gorodkova</name></author><author><name sortKey="Klenk, H D" sort="Klenk, H D" uniqKey="Klenk H" first="H D" last="Klenk">H D Klenk</name></author><author><name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal</term><term>Antibodies, Viral (blood)</term><term>Base Sequence</term><term>Blotting, Western</term><term>Ferrets</term><term>Humans</term><term>Immunoenzyme Techniques</term><term>Influenza A virus (immunology)</term><term>Molecular Sequence Data</term><term>Moths</term><term>Recombinant Proteins (immunology)</term><term>Viral Matrix Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Anticorps monoclonaux</term><term>Données de séquences moléculaires</term><term>Furets</term><term>Humains</term><term>Papillons de nuit</term><term>Protéines de la matrice virale (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Séquence nucléotidique</term><term>Technique de Western</term><term>Techniques immunoenzymatiques</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Recombinant Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Blotting, Western</term><term>Ferrets</term><term>Humans</term><term>Immunoenzyme Techniques</term><term>Molecular Sequence Data</term><term>Moths</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Données de séquences moléculaires</term><term>Furets</term><term>Humains</term><term>Papillons de nuit</term><term>Séquence nucléotidique</term><term>Technique de Western</term><term>Techniques immunoenzymatiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS-polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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