Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses.
Identifieur interne : 001697 ( Main/Merge ); précédent : 001696; suivant : 001698Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses.
Auteurs : I. Kiseleva [États-Unis] ; Q. Su ; T J Toner ; C. Szymkowiak ; W-S Kwan ; L. Rudenko ; A R Shaw ; R. YouilSource :
- Journal of virological methods [ 0166-0934 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Basse température, Chiens, Embryon de poulet, Lignée cellulaire, Phénotype, Réplication virale, Température, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A (génétique), Virus de la grippe A (pathogénicité), Virus de la grippe A (physiologie), Virus influenza B (pathogénicité), Virus influenza B (physiologie), Virus recombinants (génétique), Virus recombinants (physiologie).
- MESH :
- génétique : Virus de la grippe A, Virus recombinants.
- pathogénicité : Virus de la grippe A, Virus influenza B.
- physiologie : Virus de la grippe A, Virus influenza B, Virus recombinants.
- Animaux, Basse température, Chiens, Embryon de poulet, Lignée cellulaire, Phénotype, Réplication virale, Température, Vaccins antigrippaux, Vaccins atténués.
English descriptors
- KwdEn :
- Animals, Cell Line, Chick Embryo, Cold Temperature, Dogs, Influenza A virus (genetics), Influenza A virus (pathogenicity), Influenza A virus (physiology), Influenza B virus (pathogenicity), Influenza B virus (physiology), Influenza Vaccines, Phenotype, Reassortant Viruses (genetics), Reassortant Viruses (physiology), Temperature, Vaccines, Attenuated, Virus Replication.
- MESH :
- chemical : Influenza Vaccines, Vaccines, Attenuated.
- genetics : Influenza A virus, Reassortant Viruses.
- pathogenicity : Influenza A virus, Influenza B virus.
- physiology : Influenza A virus, Influenza B virus, Reassortant Viruses.
- Animals, Cell Line, Chick Embryo, Cold Temperature, Dogs, Phenotype, Temperature, Virus Replication.
Abstract
The determination of temperature sensitive (ts) and cold adapted (ca) phenotype for influenza A and B strains has been conducted traditionally using embryonated chicken eggs. As attempts are made to move away from the use of eggs in the manufacturing process of influenza vaccines, it will become useful to develop cell-based assays to support cell culture-based vaccine production. In this study, MDCK cells have been evaluated as a tool for determining the ts and ca phenotypes associated with live attenuated influenza viruses. Direct comparisons were made of these phenotypes carried out in eggs. Reassortants made from the Russian live attenuated influenza donor strains A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 were prepared entirely in MDCK cells and their phenotypes evaluated using the MDCK cell-based assay. It is concluded that MDCK cells are more sensitive than eggs for the measurement of ts and ca phenotype of influenza viruses (particularly for influenza A) and they provide an alternative means for screening candidate reassortants prior to determining their genome composition.
DOI: 10.1016/j.jviromet.2003.10.012
PubMed: 14715309
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pubmed:14715309Le document en format XML
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Su</name></author><author><name sortKey="Toner, T J" sort="Toner, T J" uniqKey="Toner T" first="T J" last="Toner">T J Toner</name></author><author><name sortKey="Szymkowiak, C" sort="Szymkowiak, C" uniqKey="Szymkowiak C" first="C" last="Szymkowiak">C. Szymkowiak</name></author><author><name sortKey="Kwan, W S" sort="Kwan, W S" uniqKey="Kwan W" first="W-S" last="Kwan">W-S Kwan</name></author><author><name sortKey="Rudenko, L" sort="Rudenko, L" uniqKey="Rudenko L" first="L" last="Rudenko">L. Rudenko</name></author><author><name sortKey="Shaw, A R" sort="Shaw, A R" uniqKey="Shaw A" first="A R" last="Shaw">A R Shaw</name></author><author><name sortKey="Youil, R" sort="Youil, R" uniqKey="Youil R" first="R" last="Youil">R. 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Kiseleva</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virus and Cell Biology, Merck & Co Inc, 770 Sumneytown Pike, WP44L-206B, West Point, PA 19486, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Virus and Cell Biology, Merck & Co Inc, 770 Sumneytown Pike, WP44L-206B, West Point, PA 19486</wicri:regionArea><wicri:noRegion>PA 19486</wicri:noRegion></affiliation></author><author><name sortKey="Su, Q" sort="Su, Q" uniqKey="Su Q" first="Q" last="Su">Q. Su</name></author><author><name sortKey="Toner, T J" sort="Toner, T J" uniqKey="Toner T" first="T J" last="Toner">T J Toner</name></author><author><name sortKey="Szymkowiak, C" sort="Szymkowiak, C" uniqKey="Szymkowiak C" first="C" last="Szymkowiak">C. 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Youil</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Dogs</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (pathogenicity)</term><term>Influenza A virus (physiology)</term><term>Influenza B virus (pathogenicity)</term><term>Influenza B virus (physiology)</term><term>Influenza Vaccines</term><term>Phenotype</term><term>Reassortant Viruses (genetics)</term><term>Reassortant Viruses (physiology)</term><term>Temperature</term><term>Vaccines, Attenuated</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Chiens</term><term>Embryon de poulet</term><term>Lignée cellulaire</term><term>Phénotype</term><term>Réplication virale</term><term>Température</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (pathogénicité)</term><term>Virus de la grippe A (physiologie)</term><term>Virus influenza B (pathogénicité)</term><term>Virus influenza B (physiologie)</term><term>Virus recombinants (génétique)</term><term>Virus recombinants (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Dogs</term><term>Phenotype</term><term>Temperature</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Chiens</term><term>Embryon de poulet</term><term>Lignée cellulaire</term><term>Phénotype</term><term>Réplication virale</term><term>Température</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The determination of temperature sensitive (ts) and cold adapted (ca) phenotype for influenza A and B strains has been conducted traditionally using embryonated chicken eggs. As attempts are made to move away from the use of eggs in the manufacturing process of influenza vaccines, it will become useful to develop cell-based assays to support cell culture-based vaccine production. In this study, MDCK cells have been evaluated as a tool for determining the ts and ca phenotypes associated with live attenuated influenza viruses. Direct comparisons were made of these phenotypes carried out in eggs. Reassortants made from the Russian live attenuated influenza donor strains A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 were prepared entirely in MDCK cells and their phenotypes evaluated using the MDCK cell-based assay. It is concluded that MDCK cells are more sensitive than eggs for the measurement of ts and ca phenotype of influenza viruses (particularly for influenza A) and they provide an alternative means for screening candidate reassortants prior to determining their genome composition.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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