Molecular detection of a novel human influenza (H1N1) of pandemic potential by conventional and real-time quantitative RT-PCR assays.
Identifieur interne : 001077 ( Main/Merge ); précédent : 001076; suivant : 001078Molecular detection of a novel human influenza (H1N1) of pandemic potential by conventional and real-time quantitative RT-PCR assays.
Auteurs : Leo L M. Poon [République populaire de Chine] ; K H Chan ; G J Smith ; C S W. Leung ; Y. Guan ; K Y Yuen ; J S M. PeirisSource :
- Clinical chemistry [ 1530-8561 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN viral (analyse), ADN viral (génétique), ARN viral (analyse), ARN viral (génétique), ARN viral (isolement et purification), Animaux, Facteurs temps, Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Infections à Orthomyxoviridae (diagnostic), Infections à Orthomyxoviridae (virologie), RT-PCR (), RT-PCR (économie), Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A (), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (isolement et purification), Sous-type H2N2 du virus de la grippe A (), Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (isolement et purification), Sous-type H3N2 du virus de la grippe A (), Sous-type H3N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (isolement et purification), Sous-type H5N1 du virus de la grippe A (), Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (isolement et purification), Suidae (virologie), Séquence nucléotidique, Techniques de diagnostic moléculaire (), Techniques de diagnostic moléculaire (économie).
- MESH :
- analyse : ADN viral, ARN viral.
- diagnostic : Grippe humaine, Infections à Orthomyxoviridae.
- génétique : ADN viral, ARN viral, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- isolement et purification : ARN viral, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- virologie : Grippe humaine, Infections à Orthomyxoviridae, Suidae.
- économie : RT-PCR, Techniques de diagnostic moléculaire.
- Animaux, Facteurs temps, Humains, RT-PCR, Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A, Séquence nucléotidique, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Animals, Base Sequence, DNA, Viral (analysis), DNA, Viral (genetics), Humans, Influenza A Virus, H1N1 Subtype (classification), Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza A Virus, H2N2 Subtype (classification), Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (isolation & purification), Influenza A Virus, H3N2 Subtype (classification), Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (isolation & purification), Influenza A Virus, H5N1 Subtype (classification), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Molecular Diagnostic Techniques (economics), Molecular Diagnostic Techniques (methods), Orthomyxoviridae Infections (diagnosis), Orthomyxoviridae Infections (virology), RNA, Viral (analysis), RNA, Viral (genetics), RNA, Viral (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (economics), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Swine (virology), Time Factors.
- MESH :
- chemical , analysis : DNA, Viral, RNA, Viral.
- chemical , genetics : DNA, Viral, RNA, Viral.
- classification : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype.
- diagnosis : Influenza, Human, Orthomyxoviridae Infections.
- economics : Molecular Diagnostic Techniques, Reverse Transcriptase Polymerase Chain Reaction.
- genetics : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype, RNA, Viral.
- methods : Molecular Diagnostic Techniques, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human, Orthomyxoviridae Infections, Swine.
- Animals, Base Sequence, Humans, Sensitivity and Specificity, Time Factors.
Abstract
Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed.
Url:
DOI: 10.1373/clinchem.2009.130229
PubMed: 19439731
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Links to Exploration step
pubmed:19439731Le document en format XML
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<term>Influenza A Virus, H1N1 Subtype (classification)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term>
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<term>RNA, Viral (isolation & purification)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (economics)</term>
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<term>Grippe humaine (virologie)</term>
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<term>Infections à Orthomyxoviridae (virologie)</term>
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<front><div type="abstract" xml:lang="en">Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed.</div>
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<author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name>
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<author><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K. H." last="Chan">K. H. Chan</name>
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<author><name sortKey="Smith, G J" sort="Smith, G J" uniqKey="Smith G" first="G. J." last="Smith">G. J. Smith</name>
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<author><name sortKey="Leung, C S W" sort="Leung, C S W" uniqKey="Leung C" first="C. S. W." last="Leung">C. S. W. Leung</name>
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<author><name sortKey="Guan, Y" sort="Guan, Y" uniqKey="Guan Y" first="Y." last="Guan">Y. Guan</name>
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</affiliation>
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<author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y." last="Yuen">K. Y. Yuen</name>
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<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
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<idno type="DOI">10.1373/clinchem.2009.130229</idno>
<series><title level="j">Clinical Chemistry</title>
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<front><div type="abstract" xml:lang="en"> <p>BACKGROUND: Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed. METHODS: We developed a conventional 1-step RT-PCR assay and a 1-step quantitative real-time RT-PCR assay to detect the novel H1N1 virus, but not the seasonal H1N1 viruses. We also developed an additional real-time RT-PCR that can discriminate the novel H1N1 from other swine and human H1 subtype viruses. RESULTS: All of the assays had detection limits for the positive control in the range of 1.0 x 10(-4) to 2.0 x 10(-3) of the median tissue culture infective dose. Assay specificities were high, and for the conventional and real-time assays, all negative control samples were negative, including 7 human seasonal H1N1 viruses, 1 human H2N2 virus, 2 human seasonal H3N2 viruses, 1 human H5N1 virus, 7 avian influenza viruses (HA subtypes 4, 5, 7, 8, 9, and 10), and 48 nasopharyngeal aspirates (NPAs) from patients with noninfluenza respiratory diseases; for the assay that discriminates the novel H1N1 from other swine and human H1 subtype viruses, all negative controls were also negative, including 20 control NPAs, 2 seasonal human H1N1 viruses, 2 seasonal human H3N2 viruses, and 2 human H5N1 viruses. CONCLUSIONS: These assays appear useful for the rapid diagnosis of cases with the novel H1N1 virus, thereby allowing better pandemic preparedness.</p>
</div>
</front>
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<author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea>
<wicri:noRegion>Hong Kong SAR</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K H" last="Chan">K H Chan</name>
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<author><name sortKey="Smith, G J" sort="Smith, G J" uniqKey="Smith G" first="G J" last="Smith">G J Smith</name>
</author>
<author><name sortKey="Leung, C S W" sort="Leung, C S W" uniqKey="Leung C" first="C S W" last="Leung">C S W. Leung</name>
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<author><name sortKey="Guan, Y" sort="Guan, Y" uniqKey="Guan Y" first="Y" last="Guan">Y. Guan</name>
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<author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name>
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<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name>
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<country xml:lang="fr">République populaire de Chine</country>
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</affiliation>
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<author><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K H" last="Chan">K H Chan</name>
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<author><name sortKey="Smith, G J" sort="Smith, G J" uniqKey="Smith G" first="G J" last="Smith">G J Smith</name>
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<author><name sortKey="Guan, Y" sort="Guan, Y" uniqKey="Guan Y" first="Y" last="Guan">Y. Guan</name>
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<author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name>
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<idno type="eISSN">1530-8561</idno>
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<term>Base Sequence</term>
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<term>DNA, Viral (genetics)</term>
<term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype (classification)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term>
<term>Influenza A Virus, H2N2 Subtype (classification)</term>
<term>Influenza A Virus, H2N2 Subtype (genetics)</term>
<term>Influenza A Virus, H2N2 Subtype (isolation & purification)</term>
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<term>Influenza A Virus, H3N2 Subtype (isolation & purification)</term>
<term>Influenza A Virus, H5N1 Subtype (classification)</term>
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<term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
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<term>Molecular Diagnostic Techniques (economics)</term>
<term>Molecular Diagnostic Techniques (methods)</term>
<term>Orthomyxoviridae Infections (diagnosis)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (economics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
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<term>ARN viral (génétique)</term>
<term>ARN viral (isolement et purification)</term>
<term>Animaux</term>
<term>Facteurs temps</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
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<term>Base Sequence</term>
<term>Humans</term>
<term>Sensitivity and Specificity</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Facteurs temps</term>
<term>Humains</term>
<term>RT-PCR</term>
<term>Sensibilité et spécificité</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Séquence nucléotidique</term>
<term>Techniques de diagnostic moléculaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed.</div>
</front>
</TEI>
</PubMed>
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