The Receptor Binding Specificity of the Live Attenuated Influenza H2 and H6 Vaccine Viruses Contributes to Vaccine Immunogenicity and Protection in Ferrets
Identifieur interne : 000A07 ( Main/Merge ); précédent : 000A06; suivant : 000A08The Receptor Binding Specificity of the Live Attenuated Influenza H2 and H6 Vaccine Viruses Contributes to Vaccine Immunogenicity and Protection in Ferrets
Auteurs : Zhongying Chen ; Helen Zhou ; Lomi Kim ; Hong JinSource :
- Journal of Virology [ 0022-538X ] ; 2012.
Abstract
To prepare for influenza pandemics that may be caused by the H2 and H6 subtype influenza viruses, live attenuated influenza virus (LAIV) H2 and H6 vaccines are being developed and evaluated. The H2 and H6 vaccine candidates with different receptor binding preferences specified by amino acid substitutions at residues 226 and 228 were generated and evaluated for their growth in embryonated chicken eggs and their immunogenicity and protection against wild-type virus challenge in the ferret model. The viruses containing Q226 and G228 in the hemagglutinin (HA) protein bound to the avian-like α2,3-sialic acid (SA) receptor and replicated efficiently in chicken eggs. The viruses with L226 and G228 bound preferentially to the human-like α2,6-SA receptor. The viruses containing L226 and S228 displayed dual binding to both α2,3-SA and α2,6-SA receptors and replicated efficiently in eggs. The strains containing L226/G228 or L226/S228 that preferentially bound to α2,6-SA receptors replicated efficiently in the upper respiratory tract of ferrets, induced high levels of neutralizing antibody, and conferred a high level of protection against wild-type virus challenge infection compared to the strain with the Q226/G228 residues. Our data suggest that pandemic vaccines with receptor binding preference to both avian- and human-like receptors might be desired for efficient viral replication in eggs and for inducing protective immune responses in humans.
Url:
DOI: 10.1128/JVI.06219-11
PubMed: 22190726
PubMed Central: 3302243
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000643
- to stream Pmc, to step Curation: 000643
- to stream Pmc, to step Checkpoint: 000672
- to stream Ncbi, to step Merge: 000724
- to stream Ncbi, to step Curation: 000724
- to stream Ncbi, to step Checkpoint: 000724
Links to Exploration step
PMC:3302243Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Receptor Binding Specificity of the Live Attenuated Influenza H2 and H6 Vaccine Viruses Contributes to Vaccine Immunogenicity and Protection in Ferrets</title>
<author><name sortKey="Chen, Zhongying" sort="Chen, Zhongying" uniqKey="Chen Z" first="Zhongying" last="Chen">Zhongying Chen</name>
</author>
<author><name sortKey="Zhou, Helen" sort="Zhou, Helen" uniqKey="Zhou H" first="Helen" last="Zhou">Helen Zhou</name>
</author>
<author><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name>
</author>
<author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22190726</idno>
<idno type="pmc">3302243</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302243</idno>
<idno type="RBID">PMC:3302243</idno>
<idno type="doi">10.1128/JVI.06219-11</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000643</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000643</idno>
<idno type="wicri:Area/Pmc/Curation">000643</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000643</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000672</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000672</idno>
<idno type="wicri:Area/Ncbi/Merge">000724</idno>
<idno type="wicri:Area/Ncbi/Curation">000724</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000724</idno>
<idno type="wicri:doubleKey">0022-538X:2012:Chen Z:the:receptor:binding</idno>
<idno type="wicri:Area/Main/Merge">000A07</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Receptor Binding Specificity of the Live Attenuated Influenza H2 and H6 Vaccine Viruses Contributes to Vaccine Immunogenicity and Protection in Ferrets</title>
<author><name sortKey="Chen, Zhongying" sort="Chen, Zhongying" uniqKey="Chen Z" first="Zhongying" last="Chen">Zhongying Chen</name>
</author>
<author><name sortKey="Zhou, Helen" sort="Zhou, Helen" uniqKey="Zhou H" first="Helen" last="Zhou">Helen Zhou</name>
</author>
<author><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name>
</author>
<author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>To prepare for influenza pandemics that may be caused by the H2 and H6 subtype influenza viruses, live attenuated influenza virus (LAIV) H2 and H6 vaccines are being developed and evaluated. The H2 and H6 vaccine candidates with different receptor binding preferences specified by amino acid substitutions at residues 226 and 228 were generated and evaluated for their growth in embryonated chicken eggs and their immunogenicity and protection against wild-type virus challenge in the ferret model. The viruses containing Q226 and G228 in the hemagglutinin (HA) protein bound to the avian-like α2,3-sialic acid (SA) receptor and replicated efficiently in chicken eggs. The viruses with L226 and G228 bound preferentially to the human-like α2,6-SA receptor. The viruses containing L226 and S228 displayed dual binding to both α2,3-SA and α2,6-SA receptors and replicated efficiently in eggs. The strains containing L226/G228 or L226/S228 that preferentially bound to α2,6-SA receptors replicated efficiently in the upper respiratory tract of ferrets, induced high levels of neutralizing antibody, and conferred a high level of protection against wild-type virus challenge infection compared to the strain with the Q226/G228 residues. Our data suggest that pandemic vaccines with receptor binding preference to both avian- and human-like receptors might be desired for efficient viral replication in eggs and for inducing protective immune responses in humans.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A07 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000A07 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Merge |type= RBID |clé= PMC:3302243 |texte= The Receptor Binding Specificity of the Live Attenuated Influenza H2 and H6 Vaccine Viruses Contributes to Vaccine Immunogenicity and Protection in Ferrets }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:22190726" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |