In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
Identifieur interne : 000354 ( Main/Merge ); précédent : 000353; suivant : 000355In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
Auteurs : Nicholas C. Wu [États-Unis] ; Geramie Grande [États-Unis] ; Hannah L. Turner [États-Unis] ; Andrew B. Ward [États-Unis] ; Jia Xie [États-Unis] ; Richard A. Lerner [États-Unis] ; Ian A. Wilson [États-Unis]Source :
- Nature Communications [ 2041-1723 ] ; 2017.
Abstract
The relatively recent discovery and characterization of human broadly neutralizing antibodies (bnAbs) against influenza virus provide valuable insights into antiviral and vaccine development. However, the factors that influence the evolution of high-affinity bnAbs remain elusive. We therefore explore the functional sequence space of bnAb C05, which targets the receptor-binding site (RBS) of influenza haemagglutinin (HA) via a long CDR H3. We combine saturation mutagenesis with yeast display to enrich for C05 variants of CDR H3 that bind to H1 and H3 HAs. The C05 variants evolve up to 20-fold higher affinity but increase specificity to each HA subtype used in the selection. Structural analysis reveals that the fine specificity is strongly influenced by a highly conserved substitution that regulates receptor binding in different subtypes. Overall, this study suggests that subtle natural variations in the HA RBS between subtypes and species may differentially influence the evolution of high-affinity bnAbs.
Url:
DOI: 10.1038/ncomms15371
PubMed: 28504265
PubMed Central: 5440694
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000096
- to stream Pmc, to step Curation: 000096
- to stream Pmc, to step Checkpoint: 000272
- to stream Ncbi, to step Merge: 000D78
- to stream Ncbi, to step Curation: 000D78
- to stream Ncbi, to step Checkpoint: 000D78
Links to Exploration step
PMC:5440694Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In vitro</italic>
evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity</title>
<author><name sortKey="Wu, Nicholas C" sort="Wu, Nicholas C" uniqKey="Wu N" first="Nicholas C." last="Wu">Nicholas C. Wu</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Grande, Geramie" sort="Grande, Geramie" uniqKey="Grande G" first="Geramie" last="Grande">Geramie Grande</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Turner, Hannah L" sort="Turner, Hannah L" uniqKey="Turner H" first="Hannah L." last="Turner">Hannah L. Turner</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ward, Andrew B" sort="Ward, Andrew B" uniqKey="Ward A" first="Andrew B." last="Ward">Andrew B. Ward</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xie, Jia" sort="Xie, Jia" uniqKey="Xie J" first="Jia" last="Xie">Jia Xie</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lerner, Richard A" sort="Lerner, Richard A" uniqKey="Lerner R" first="Richard A." last="Lerner">Richard A. Lerner</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wilson, Ian A" sort="Wilson, Ian A" uniqKey="Wilson I" first="Ian A." last="Wilson">Ian A. Wilson</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>The Skaggs Institute for Chemical Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">28504265</idno>
<idno type="pmc">5440694</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440694</idno>
<idno type="RBID">PMC:5440694</idno>
<idno type="doi">10.1038/ncomms15371</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000096</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000096</idno>
<idno type="wicri:Area/Pmc/Curation">000096</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000096</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000272</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000272</idno>
<idno type="wicri:Area/Ncbi/Merge">000D78</idno>
<idno type="wicri:Area/Ncbi/Curation">000D78</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000D78</idno>
<idno type="wicri:Area/Main/Merge">000354</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vitro</italic>
evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity</title>
<author><name sortKey="Wu, Nicholas C" sort="Wu, Nicholas C" uniqKey="Wu N" first="Nicholas C." last="Wu">Nicholas C. Wu</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Grande, Geramie" sort="Grande, Geramie" uniqKey="Grande G" first="Geramie" last="Grande">Geramie Grande</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Turner, Hannah L" sort="Turner, Hannah L" uniqKey="Turner H" first="Hannah L." last="Turner">Hannah L. Turner</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ward, Andrew B" sort="Ward, Andrew B" uniqKey="Ward A" first="Andrew B." last="Ward">Andrew B. Ward</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xie, Jia" sort="Xie, Jia" uniqKey="Xie J" first="Jia" last="Xie">Jia Xie</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lerner, Richard A" sort="Lerner, Richard A" uniqKey="Lerner R" first="Richard A." last="Lerner">Richard A. Lerner</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Chemistry, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wilson, Ian A" sort="Wilson, Ian A" uniqKey="Wilson I" first="Ian A." last="Wilson">Ian A. Wilson</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Integrative Structural and Computational Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>The Skaggs Institute for Chemical Biology, The Scripps Research Institute</institution>
, La Jolla, California 92037,<country>USA</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature Communications</title>
<idno type="eISSN">2041-1723</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The relatively recent discovery and characterization of human broadly neutralizing antibodies (bnAbs) against influenza virus provide valuable insights into antiviral and vaccine development. However, the factors that influence the evolution of high-affinity bnAbs remain elusive. We therefore explore the functional sequence space of bnAb C05, which targets the receptor-binding site (RBS) of influenza haemagglutinin (HA) via a long CDR H3. We combine saturation mutagenesis with yeast display to enrich for C05 variants of CDR H3 that bind to H1 and H3 HAs. The C05 variants evolve up to 20-fold higher affinity but increase specificity to each HA subtype used in the selection. Structural analysis reveals that the fine specificity is strongly influenced by a highly conserved substitution that regulates receptor binding in different subtypes. Overall, this study suggests that subtle natural variations in the HA RBS between subtypes and species may differentially influence the evolution of high-affinity bnAbs.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Neher, R A" uniqKey="Neher R">R. A. Neher</name>
</author>
<author><name sortKey="Bedford, T" uniqKey="Bedford T">T. Bedford</name>
</author>
<author><name sortKey="Daniels, R S" uniqKey="Daniels R">R. S. Daniels</name>
</author>
<author><name sortKey="Russell, C A" uniqKey="Russell C">C. A. Russell</name>
</author>
<author><name sortKey="Shraiman, B I" uniqKey="Shraiman B">B. I. Shraiman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tricco, A C" uniqKey="Tricco A">A. C. Tricco</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hurt, A C" uniqKey="Hurt A">A. C. Hurt</name>
</author>
<author><name sortKey="Holien, J K" uniqKey="Holien J">J. K. Holien</name>
</author>
<author><name sortKey="Parker, M W" uniqKey="Parker M">M. W. Parker</name>
</author>
<author><name sortKey="Barr, I G" uniqKey="Barr I">I. G. Barr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furuse, Y" uniqKey="Furuse Y">Y. Furuse</name>
</author>
<author><name sortKey="Suzuki, A" uniqKey="Suzuki A">A. Suzuki</name>
</author>
<author><name sortKey="Oshitani, H" uniqKey="Oshitani H">H. Oshitani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Throsby, M" uniqKey="Throsby M">M. Throsby</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekiert, D C" uniqKey="Ekiert D">D. C. Ekiert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekiert, D C" uniqKey="Ekiert D">D. C. Ekiert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dreyfus, C" uniqKey="Dreyfus C">C. Dreyfus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dreyfus, C" uniqKey="Dreyfus C">C. Dreyfus</name>
</author>
<author><name sortKey="Ekiert, D C" uniqKey="Ekiert D">D. C. Ekiert</name>
</author>
<author><name sortKey="Wilson, I A" uniqKey="Wilson I">I. A. Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kashyap, A K" uniqKey="Kashyap A">A. K. Kashyap</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kallewaard, N L" uniqKey="Kallewaard N">N. L. Kallewaard</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fleishman, S J" uniqKey="Fleishman S">S. J. Fleishman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Whitehead, T A" uniqKey="Whitehead T">T. A. Whitehead</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koday, M T" uniqKey="Koday M">M. T. Koday</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Impagliazzo, A" uniqKey="Impagliazzo A">A. Impagliazzo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yassine, H M" uniqKey="Yassine H">H. M. Yassine</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Valkenburg, S A" uniqKey="Valkenburg S">S. A. Valkenburg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekiert, D C" uniqKey="Ekiert D">D. C. Ekiert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yoshida, R" uniqKey="Yoshida R">R. Yoshida</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, P S" uniqKey="Lee P">P. S. Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, P S" uniqKey="Lee P">P. S. Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ohshima, N" uniqKey="Ohshima N">N. Ohshima</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krause, J C" uniqKey="Krause J">J. C. Krause</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, R" uniqKey="Xu R">R. Xu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Whittle, J R" uniqKey="Whittle J">J. R. Whittle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hong, M" uniqKey="Hong M">M. Hong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krause, J C" uniqKey="Krause J">J. C. Krause</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, P S" uniqKey="Lee P">P. S. Lee</name>
</author>
<author><name sortKey="Wilson, I A" uniqKey="Wilson I">I. A. Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Skehel, J J" uniqKey="Skehel J">J. J. Skehel</name>
</author>
<author><name sortKey="Wiley, D C" uniqKey="Wiley D">D. C. Wiley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, J H" uniqKey="Kim J">J. H. Kim</name>
</author>
<author><name sortKey="Skountzou, I" uniqKey="Skountzou I">I. Skountzou</name>
</author>
<author><name sortKey="Compans, R" uniqKey="Compans R">R. Compans</name>
</author>
<author><name sortKey="Jacob, J" uniqKey="Jacob J">J. Jacob</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Choi, Y S" uniqKey="Choi Y">Y. S. Choi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mclean, H Q" uniqKey="Mclean H">H. Q. McLean</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Andrews, S F" uniqKey="Andrews S">S. F. Andrews</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wrammert, J" uniqKey="Wrammert J">J. Wrammert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoa Le, N M" uniqKey="Hoa Le N">N. M. Hoa le</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Packer, M S" uniqKey="Packer M">M. S. Packer</name>
</author>
<author><name sortKey="Liu, D R" uniqKey="Liu D">D. R. Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gera, N" uniqKey="Gera N">N. Gera</name>
</author>
<author><name sortKey="Hussain, M" uniqKey="Hussain M">M. Hussain</name>
</author>
<author><name sortKey="Rao, B M" uniqKey="Rao B">B. M. Rao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilmot, C M" uniqKey="Wilmot C">C. M. Wilmot</name>
</author>
<author><name sortKey="Thornton, J M" uniqKey="Thornton J">J. M. Thornton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, N C" uniqKey="Wu N">N. C. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thyagarajan, B" uniqKey="Thyagarajan B">B. Thyagarajan</name>
</author>
<author><name sortKey="Bloom, J D" uniqKey="Bloom J">J. D. Bloom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glaser, L" uniqKey="Glaser L">L. Glaser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nobusawa, E" uniqKey="Nobusawa E">E. Nobusawa</name>
</author>
<author><name sortKey="Ishihara, H" uniqKey="Ishihara H">H. Ishihara</name>
</author>
<author><name sortKey="Morishita, T" uniqKey="Morishita T">T. Morishita</name>
</author>
<author><name sortKey="Sato, K" uniqKey="Sato K">K. Sato</name>
</author>
<author><name sortKey="Nakajima, K" uniqKey="Nakajima K">K. Nakajima</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A. Pekosz</name>
</author>
<author><name sortKey="Newby, C" uniqKey="Newby C">C. Newby</name>
</author>
<author><name sortKey="Bose, P S" uniqKey="Bose P">P. S. Bose</name>
</author>
<author><name sortKey="Lutz, A" uniqKey="Lutz A">A. Lutz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matrosovich, M" uniqKey="Matrosovich M">M. Matrosovich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vines, A" uniqKey="Vines A">A. Vines</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, R" uniqKey="Xu R">R. Xu</name>
</author>
<author><name sortKey="Mcbride, R" uniqKey="Mcbride R">R. McBride</name>
</author>
<author><name sortKey="Paulson, J C" uniqKey="Paulson J">J. C. Paulson</name>
</author>
<author><name sortKey="Basler, C F" uniqKey="Basler C">C. F. Basler</name>
</author>
<author><name sortKey="Wilson, I A" uniqKey="Wilson I">I. A. Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ha, Y" uniqKey="Ha Y">Y. Ha</name>
</author>
<author><name sortKey="Stevens, D J" uniqKey="Stevens D">D. J. Stevens</name>
</author>
<author><name sortKey="Skehel, J J" uniqKey="Skehel J">J. J. Skehel</name>
</author>
<author><name sortKey="Wiley, D C" uniqKey="Wiley D">D. C. Wiley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lin, Y P" uniqKey="Lin Y">Y. P. Lin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nakamura, G" uniqKey="Nakamura G">G. Nakamura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, X" uniqKey="Wu X">X. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scheid, J F" uniqKey="Scheid J">J. F. Scheid</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pappas, L" uniqKey="Pappas L">L. Pappas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lerner, R A" uniqKey="Lerner R">R. A. Lerner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klein, J S" uniqKey="Klein J">J. S. Klein</name>
</author>
<author><name sortKey="Bjorkman, P J" uniqKey="Bjorkman P">P. J. Bjorkman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krammer, F" uniqKey="Krammer F">F. Krammer</name>
</author>
<author><name sortKey="Pica, N" uniqKey="Pica N">N. Pica</name>
</author>
<author><name sortKey="Hai, R" uniqKey="Hai R">R. Hai</name>
</author>
<author><name sortKey="Margine, I" uniqKey="Margine I">I. Margine</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P. Palese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Otwinowski, Z" uniqKey="Otwinowski Z">Z. Otwinowski</name>
</author>
<author><name sortKey="Minor, W" uniqKey="Minor W">W. Minor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mccoy, A J" uniqKey="Mccoy A">A. J. McCoy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P. Emsley</name>
</author>
<author><name sortKey="Lohkamp, B" uniqKey="Lohkamp B">B. Lohkamp</name>
</author>
<author><name sortKey="Scott, W G" uniqKey="Scott W">W. G. Scott</name>
</author>
<author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K. Cowtan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murshudov, G N" uniqKey="Murshudov G">G. N. Murshudov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, V B" uniqKey="Chen V">V. B. Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suloway, C" uniqKey="Suloway C">C. Suloway</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lander, G C" uniqKey="Lander G">G. C. Lander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Voss, N R" uniqKey="Voss N">N. R. Voss</name>
</author>
<author><name sortKey="Yoshioka, C K" uniqKey="Yoshioka C">C. K. Yoshioka</name>
</author>
<author><name sortKey="Radermacher, M" uniqKey="Radermacher M">M. Radermacher</name>
</author>
<author><name sortKey="Potter, C S" uniqKey="Potter C">C. S. Potter</name>
</author>
<author><name sortKey="Carragher, B" uniqKey="Carragher B">B. Carragher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, G" uniqKey="Tang G">G. Tang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crooks, G E" uniqKey="Crooks G">G. E. Crooks</name>
</author>
<author><name sortKey="Hon, G" uniqKey="Hon G">G. Hon</name>
</author>
<author><name sortKey="Chandonia, J M" uniqKey="Chandonia J">J. M. Chandonia</name>
</author>
<author><name sortKey="Brenner, S E" uniqKey="Brenner S">S. E. Brenner</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000354 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000354 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Merge |type= RBID |clé= PMC:5440694 |texte= In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:28504265" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |