Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.
Identifieur interne : 002A43 ( Main/Exploration ); précédent : 002A42; suivant : 002A44Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.
Auteurs : A P Kendal ; N J Cox ; B R Murphy ; S B Spring ; H F MaassabSource :
- The Journal of general virology [ 0022-1317 ] ; 1977.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Adaptation biologique, Basse température, Gènes viraux, Mutation, Peptides (analyse), Protéines virales (analyse), Protéines virales (génétique), Recombinaison génétique, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A (analyse), Virus de la grippe A (croissance et développement), Virus de la grippe A (génétique).
- MESH :
- analyse : ARN viral, Peptides, Protéines virales, Virus de la grippe A.
- croissance et développement : Virus de la grippe A.
- génétique : Protéines virales, Virus de la grippe A.
- Adaptation biologique, Basse température, Gènes viraux, Mutation, Recombinaison génétique, Sous-type H3N2 du virus de la grippe A.
English descriptors
- KwdEn :
- Adaptation, Biological, Cold Temperature, Genes, Viral, Influenza A Virus, H3N2 Subtype, Influenza A virus (analysis), Influenza A virus (genetics), Influenza A virus (growth & development), Mutation, Peptides (analysis), RNA, Viral (analysis), Recombination, Genetic, Viral Proteins (analysis), Viral Proteins (genetics).
- MESH :
- chemical , analysis : Peptides, RNA, Viral, Viral Proteins.
- analysis : Influenza A virus.
- genetics : Influenza A virus, Viral Proteins.
- growth & development : Influenza A virus.
- Adaptation, Biological, Cold Temperature, Genes, Viral, Influenza A Virus, H3N2 Subtype, Mutation, Recombination, Genetic.
Abstract
The matrix (M) protein of the H2N2 virus A/Ann Arbor/6/60 may be distinguished from M protein of several H3N2 viruses and A/New Jersey/76 (HSWINI) by SDS acrylamide gel electrophoresis using a discontinuous buffer system. The smallest RNA (RNA 8) of the A/Ann Arbor/6/60 virus may be distinguished from RNA 8 of several H3N2 viruses by acrylamide gel electrophoresis in 3% or 3-6% gels in the absence of urea, if electrophoresis is done at 30 to 36 degrees C or 20 degrees C respectively. Ten clones of conditionally-lethal temperature-sensitive (ts) mutants were studied, which derived their cold-adaption and ts genes from mutant A/Ann Arbor/6/60, and their haemagglutinin from the H3N2 virus A/Scotland/840/74. Each clone was found to derive its M protein from A/Ann Arbor/6/60 mutant, and its RNA 8 from A/Scotland/840/74. The only assignment of genes 7 and 8 consistent with these findings for the recombinants is that in each parent virus (and in the recombinants) gene 7 codes for M protein, and gene 8 for NS protein. Furthermore, it may be concluded from the results that the biologically important ts lesions in the A/Ann Arbor/6/60 mutant parent are not present in the NS gene. In addition to the recombinants of A/Ann Arbor/6/60 and A/Scotland/840/74, five independent ts/cold-adapted recombinants of A/Ann Arbor/6/60 mutant with H3N2 and HSWINI wild-type viruses were examined, and all were found to contain the M protein of the A/Ann Arbor/6/60 mutant parent. This is suggestive that M protein may be at least partially responsible for the cold-adaptation and/or ts properties of the A/Ann Arbor/6/60 mutant and the recombinants.
DOI: 10.1099/0022-1317-37-1-145
PubMed: 915481
Affiliations:
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Le document en format XML
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<term>Influenza A virus (analysis)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (growth & development)</term>
<term>Mutation</term>
<term>Peptides (analysis)</term>
<term>RNA, Viral (analysis)</term>
<term>Recombination, Genetic</term>
<term>Viral Proteins (analysis)</term>
<term>Viral Proteins (genetics)</term>
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<term>Adaptation biologique</term>
<term>Basse température</term>
<term>Gènes viraux</term>
<term>Mutation</term>
<term>Peptides (analyse)</term>
<term>Protéines virales (analyse)</term>
<term>Protéines virales (génétique)</term>
<term>Recombinaison génétique</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Virus de la grippe A (analyse)</term>
<term>Virus de la grippe A (croissance et développement)</term>
<term>Virus de la grippe A (génétique)</term>
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<term>RNA, Viral</term>
<term>Viral Proteins</term>
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<term>Peptides</term>
<term>Protéines virales</term>
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<term>Genes, Viral</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Mutation</term>
<term>Recombination, Genetic</term>
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<term>Basse température</term>
<term>Gènes viraux</term>
<term>Mutation</term>
<term>Recombinaison génétique</term>
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<front><div type="abstract" xml:lang="en">The matrix (M) protein of the H2N2 virus A/Ann Arbor/6/60 may be distinguished from M protein of several H3N2 viruses and A/New Jersey/76 (HSWINI) by SDS acrylamide gel electrophoresis using a discontinuous buffer system. The smallest RNA (RNA 8) of the A/Ann Arbor/6/60 virus may be distinguished from RNA 8 of several H3N2 viruses by acrylamide gel electrophoresis in 3% or 3-6% gels in the absence of urea, if electrophoresis is done at 30 to 36 degrees C or 20 degrees C respectively. Ten clones of conditionally-lethal temperature-sensitive (ts) mutants were studied, which derived their cold-adaption and ts genes from mutant A/Ann Arbor/6/60, and their haemagglutinin from the H3N2 virus A/Scotland/840/74. Each clone was found to derive its M protein from A/Ann Arbor/6/60 mutant, and its RNA 8 from A/Scotland/840/74. The only assignment of genes 7 and 8 consistent with these findings for the recombinants is that in each parent virus (and in the recombinants) gene 7 codes for M protein, and gene 8 for NS protein. Furthermore, it may be concluded from the results that the biologically important ts lesions in the A/Ann Arbor/6/60 mutant parent are not present in the NS gene. In addition to the recombinants of A/Ann Arbor/6/60 and A/Scotland/840/74, five independent ts/cold-adapted recombinants of A/Ann Arbor/6/60 mutant with H3N2 and HSWINI wild-type viruses were examined, and all were found to contain the M protein of the A/Ann Arbor/6/60 mutant parent. This is suggestive that M protein may be at least partially responsible for the cold-adaptation and/or ts properties of the A/Ann Arbor/6/60 mutant and the recombinants.</div>
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<name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name>
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