Brefeldin A implicates egress from endoplasmic reticulum in class I restricted antigen presentation
Identifieur interne : 002163 ( Main/Exploration ); précédent : 002162; suivant : 002164Brefeldin A implicates egress from endoplasmic reticulum in class I restricted antigen presentation
Auteurs : Jed G. Nuchtern [États-Unis] ; Juan S. Bonifacino [États-Unis] ; William E. Biddison ; Richard D. Klausner [États-Unis]Source :
- Nature [ 0028-0836 ] ; 1989-05-18.
Abstract
MOST antigens must be processed intracellularly before they can be presented, in association with major histocompatibility complex (MHC) molecules at the cell surface, for recognition by the antigen-specific receptor of T cells13. This processing appears to involve cleavage of protein antigens to Smaller peptides47. Only certain fragments of any protein can serve as T-cell epitopes and this is, at least in part, determined by the requirement that peptides be able to bind the MHC molecules810. Class I restricted antigens are derived from proteins, such as viral antigens, that are synthesized within the presenting cell1113. Many of these antigens are cytosolic proteins and recent evidence suggests that it is in the cytosol that these proteins are processed to produce either the antigenic peptides or processed intermediates1316. How and where these processed cytosolic antigens cross the membrane of the vacuolar system and bind to the extracellular domain of the class I molecule is not known but one obvious site for this process is the endoplasmic reticulum (ER), because this organelle is specialized to translocate proteins across the membrane from the cytosol into the secretory system. Based on this model, we reasoned that if we could pharmacologically block the movement of proteins out of the ER, endogenous antigen presentation would cease. An agent which causes such an effect is available17,18 the fungal antibiotic Brefeldin A (BFA). Consistent with the above hypothesis, we report that BFA completely abolishes the ability of a cell to present endogenously synthesized antigens to class I restricted cytotoxic T cells.
Url:
DOI: 10.1038/339223a0
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Nuchtern</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cell Biology and Metabolism Branch,National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea><wicri:noRegion>Maryland 20892</wicri:noRegion></affiliation></author><author><name sortKey="Bonifacino, Juan S" sort="Bonifacino, Juan S" uniqKey="Bonifacino J" first="Juan S." last="Bonifacino">Juan S. 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Klausner</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cell Biology and Metabolism Branch,National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea><wicri:noRegion>Maryland 20892</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nature</title><imprint><publisher>Nature Publishing Group</publisher><date when="1989-05-18">1989-05-18</date><biblScope unit="vol">339</biblScope><biblScope unit="issue">6221</biblScope><biblScope unit="page" from="223">223</biblScope><biblScope unit="page" to="226">226</biblScope><date type="Copyright" when="1989">1989</date></imprint><idno type="ISSN">0028-0836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0028-0836</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">MOST antigens must be processed intracellularly before they can be presented, in association with major histocompatibility complex (MHC) molecules at the cell surface, for recognition by the antigen-specific receptor of T cells13. This processing appears to involve cleavage of protein antigens to Smaller peptides47. Only certain fragments of any protein can serve as T-cell epitopes and this is, at least in part, determined by the requirement that peptides be able to bind the MHC molecules810. Class I restricted antigens are derived from proteins, such as viral antigens, that are synthesized within the presenting cell1113. Many of these antigens are cytosolic proteins and recent evidence suggests that it is in the cytosol that these proteins are processed to produce either the antigenic peptides or processed intermediates1316. How and where these processed cytosolic antigens cross the membrane of the vacuolar system and bind to the extracellular domain of the class I molecule is not known but one obvious site for this process is the endoplasmic reticulum (ER), because this organelle is specialized to translocate proteins across the membrane from the cytosol into the secretory system. Based on this model, we reasoned that if we could pharmacologically block the movement of proteins out of the ER, endogenous antigen presentation would cease. An agent which causes such an effect is available17,18 the fungal antibiotic Brefeldin A (BFA). Consistent with the above hypothesis, we report that BFA completely abolishes the ability of a cell to present endogenously synthesized antigens to class I restricted cytotoxic T cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Biddison, William E" sort="Biddison, William E" uniqKey="Biddison W" first="William E." last="Biddison">William E. Biddison</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Nuchtern, Jed G" sort="Nuchtern, Jed G" uniqKey="Nuchtern J" first="Jed G." last="Nuchtern">Jed G. 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